IL250949B - Biosensors based on g-beta-gamma reactive proteins for monitoring g-protein activation - Google Patents
Biosensors based on g-beta-gamma reactive proteins for monitoring g-protein activationInfo
- Publication number
- IL250949B IL250949B IL250949A IL25094917A IL250949B IL 250949 B IL250949 B IL 250949B IL 250949 A IL250949 A IL 250949A IL 25094917 A IL25094917 A IL 25094917A IL 250949 B IL250949 B IL 250949B
- Authority
- IL
- Israel
- Prior art keywords
- gamma
- beta
- monitor
- interacting proteins
- protein activation
- Prior art date
Links
- 230000004913 activation Effects 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4722—G-proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
- C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4719—G-proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
- G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462063622P | 2014-10-14 | 2014-10-14 | |
| PCT/CA2015/051032 WO2016058094A1 (en) | 2014-10-14 | 2015-10-14 | BIOSENSOR BASED ON GβΥ-INTERACTING PROTEINS TO MONITOR G-PROTEIN ACTIVATION |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL250949A0 IL250949A0 (en) | 2017-04-30 |
| IL250949B true IL250949B (en) | 2021-07-29 |
Family
ID=55745919
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL250949A IL250949B (en) | 2014-10-14 | 2017-03-05 | Biosensors based on g-beta-gamma reactive proteins for monitoring g-protein activation |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US10877036B2 (enExample) |
| EP (1) | EP3207052B1 (enExample) |
| JP (1) | JP6932636B2 (enExample) |
| CN (1) | CN107074926B (enExample) |
| AU (1) | AU2015333543B2 (enExample) |
| CA (1) | CA2963579C (enExample) |
| DK (1) | DK3207052T3 (enExample) |
| ES (1) | ES2832331T3 (enExample) |
| IL (1) | IL250949B (enExample) |
| NZ (1) | NZ729735A (enExample) |
| PL (1) | PL3207052T3 (enExample) |
| SG (1) | SG11201701895VA (enExample) |
| WO (1) | WO2016058094A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20200400567A1 (en) * | 2017-09-27 | 2020-12-24 | Peking University | Fusion polypeptide |
| WO2019076262A1 (zh) * | 2017-10-18 | 2019-04-25 | 深圳华大生命科学研究院 | 检测目标分子化合物的生物感应器及包含该生物感应器的系统 |
| KR20200071724A (ko) | 2017-10-18 | 2020-06-19 | 유니버시떼 드 몬트리얼 | G-단백질 활성화의 평가를 위한 시스템 및 방법 |
| US12360111B2 (en) * | 2018-12-04 | 2025-07-15 | The University Of North Carolina At Chapel Hill | G protein-coupled receptor screening systems |
| FR3092172B1 (fr) * | 2019-01-30 | 2021-02-12 | Cisbio Bioassays | Méthode pour mesurer la modulation de l’activation d’un récepteur couplé à une protéine G avec des analogues du GTP |
| CN110981943B (zh) * | 2019-12-02 | 2021-08-03 | 清华大学 | 多肽及其在制备药物中的用途和药物 |
| KR102778534B1 (ko) | 2022-10-07 | 2025-03-07 | 성균관대학교산학협력단 | G 단백질 알파 서브유닛의 알파-헬리컬 도메인의 실시간 구조 변화 확인 방법 및 시스템 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1532446B1 (en) * | 2002-04-12 | 2012-02-15 | Schering Corporation | G-protein coupled receptor ligands and methods |
| US20050042720A1 (en) | 2003-04-24 | 2005-02-24 | 7Tm Pharma A/S | Novel promiscuous G alpha protein mutants and their use |
| US7488583B2 (en) | 2003-09-25 | 2009-02-10 | Odyssey Thera, Inc. | Fragment complementation assays for G-protein-coupled receptors and their signaling pathways |
| WO2005121755A1 (en) | 2004-06-14 | 2005-12-22 | Commonwealth Scientific And Industrial Research Organisation | Cell free g-protein coupled receptor and ligand assay |
| GB0822259D0 (en) * | 2008-12-05 | 2009-01-14 | Ge Healthcare Uk Ltd | Methods for testing binding of a ligand to a G protein- coupled receptor |
| EP2382470B1 (en) * | 2009-01-29 | 2014-12-10 | Commonwealth Scientific and Industrial Research Organisation | Measuring g protein coupled receptor activation |
| GB0905419D0 (en) | 2009-03-30 | 2009-05-13 | Ge Healthcare Uk Ltd | Methods for testing ligand binding to G protein-coupled receptors |
| EP2639859A4 (en) | 2010-11-11 | 2015-02-18 | Ube Industries | CONTAINER FOR A NON-AQUEOUS ELECTROLYTE SOLUTION, NON-ACID ELECTROLYTE SOLUTION FOR FILLING INTO THIS CONTAINER, AND METHOD FOR STORING NON-WATER ELECTROLYTE SOLUTION |
| US20140031525A1 (en) * | 2011-01-21 | 2014-01-30 | Heptares Therapeutics Limited | Mutant g-protein coupled receptor proteins and methods for producing them |
| WO2015095973A1 (en) | 2013-12-23 | 2015-07-02 | Université de Montréal | Systems and methods for the monitoring of protein complex formation in cells |
-
2015
- 2015-10-14 US US15/518,888 patent/US10877036B2/en active Active
- 2015-10-14 CA CA2963579A patent/CA2963579C/en active Active
- 2015-10-14 JP JP2017520506A patent/JP6932636B2/ja active Active
- 2015-10-14 ES ES15851395T patent/ES2832331T3/es active Active
- 2015-10-14 EP EP15851395.2A patent/EP3207052B1/en active Active
- 2015-10-14 PL PL15851395T patent/PL3207052T3/pl unknown
- 2015-10-14 WO PCT/CA2015/051032 patent/WO2016058094A1/en not_active Ceased
- 2015-10-14 DK DK15851395.2T patent/DK3207052T3/da active
- 2015-10-14 AU AU2015333543A patent/AU2015333543B2/en active Active
- 2015-10-14 CN CN201580055471.5A patent/CN107074926B/zh active Active
- 2015-10-14 SG SG11201701895VA patent/SG11201701895VA/en unknown
- 2015-10-14 NZ NZ729735A patent/NZ729735A/en unknown
-
2017
- 2017-03-05 IL IL250949A patent/IL250949B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP3207052A1 (en) | 2017-08-23 |
| WO2016058094A1 (en) | 2016-04-21 |
| ES2832331T3 (es) | 2021-06-10 |
| AU2015333543A1 (en) | 2017-03-23 |
| CN107074926A (zh) | 2017-08-18 |
| EP3207052B1 (en) | 2020-09-30 |
| US10877036B2 (en) | 2020-12-29 |
| SG11201701895VA (en) | 2017-04-27 |
| JP2017536104A (ja) | 2017-12-07 |
| JP6932636B2 (ja) | 2021-09-08 |
| EP3207052A4 (en) | 2018-04-25 |
| CA2963579A1 (en) | 2016-04-21 |
| NZ729735A (en) | 2023-05-26 |
| CN107074926B (zh) | 2021-09-21 |
| PL3207052T3 (pl) | 2021-02-08 |
| IL250949A0 (en) | 2017-04-30 |
| AU2015333543B2 (en) | 2021-03-04 |
| CA2963579C (en) | 2023-10-31 |
| US20170234870A1 (en) | 2017-08-17 |
| DK3207052T3 (da) | 2020-11-16 |
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