JP6932636B2 - Ｇタンパク質活性化を監視するためのＧβγ相互作用タンパク質に基づいたバイオセンサー - Google Patents

Ｇタンパク質活性化を監視するためのＧβγ相互作用タンパク質に基づいたバイオセンサー Download PDF

Info

Publication number
JP6932636B2
JP6932636B2 JP2017520506A JP2017520506A JP6932636B2 JP 6932636 B2 JP6932636 B2 JP 6932636B2 JP 2017520506 A JP2017520506 A JP 2017520506A JP 2017520506 A JP2017520506 A JP 2017520506A JP 6932636 B2 JP6932636 B2 JP 6932636B2
Authority
JP
Japan
Prior art keywords
protein
fused
ret
βγip
gpcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017520506A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017536104A (ja
JP2017536104A5 (enExample
Inventor
ブビエ ミシェル
ブビエ ミシェル
ルグイユ クリスチャン
ルグイユ クリスチャン
オーゲ ミレイユ
オーゲ ミレイユ
ルカシェバ ビクトリヤ
ルカシェバ ビクトリヤ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universite de Montreal
Original Assignee
Universite de Montreal
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universite de Montreal filed Critical Universite de Montreal
Publication of JP2017536104A publication Critical patent/JP2017536104A/ja
Publication of JP2017536104A5 publication Critical patent/JP2017536104A5/ja
Application granted granted Critical
Publication of JP6932636B2 publication Critical patent/JP6932636B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4722—G-proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4719—G-proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2017520506A 2014-10-14 2015-10-14 Ｇタンパク質活性化を監視するためのＧβγ相互作用タンパク質に基づいたバイオセンサー Active JP6932636B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462063622P 2014-10-14 2014-10-14
US62/063,622 2014-10-14
PCT/CA2015/051032 WO2016058094A1 (en) 2014-10-14 2015-10-14 BIOSENSOR BASED ON GβΥ-INTERACTING PROTEINS TO MONITOR G-PROTEIN ACTIVATION

Publications (3)

Publication Number Publication Date
JP2017536104A JP2017536104A (ja) 2017-12-07
JP2017536104A5 JP2017536104A5 (enExample) 2018-10-18
JP6932636B2 true JP6932636B2 (ja) 2021-09-08

Family

ID=55745919

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017520506A Active JP6932636B2 (ja) 2014-10-14 2015-10-14 Ｇタンパク質活性化を監視するためのＧβγ相互作用タンパク質に基づいたバイオセンサー

Country Status (13)

Country Link
US (1) US10877036B2 (enExample)
EP (1) EP3207052B1 (enExample)
JP (1) JP6932636B2 (enExample)
CN (1) CN107074926B (enExample)
AU (1) AU2015333543B2 (enExample)
CA (1) CA2963579C (enExample)
DK (1) DK3207052T3 (enExample)
ES (1) ES2832331T3 (enExample)
IL (1) IL250949B (enExample)
NZ (1) NZ729735A (enExample)
PL (1) PL3207052T3 (enExample)
SG (1) SG11201701895VA (enExample)
WO (1) WO2016058094A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200400567A1 (en) * 2017-09-27 2020-12-24 Peking University Fusion polypeptide
WO2019076262A1 (zh) * 2017-10-18 2019-04-25 深圳华大生命科学研究院 检测目标分子化合物的生物感应器及包含该生物感应器的系统
KR20200071724A (ko) 2017-10-18 2020-06-19 유니버시떼 드 몬트리얼 G-단백질 활성화의 평가를 위한 시스템 및 방법
US12360111B2 (en) * 2018-12-04 2025-07-15 The University Of North Carolina At Chapel Hill G protein-coupled receptor screening systems
FR3092172B1 (fr) * 2019-01-30 2021-02-12 Cisbio Bioassays Méthode pour mesurer la modulation de l’activation d’un récepteur couplé à une protéine G avec des analogues du GTP
CN110981943B (zh) * 2019-12-02 2021-08-03 清华大学 多肽及其在制备药物中的用途和药物
KR102778534B1 (ko) 2022-10-07 2025-03-07 성균관대학교산학협력단 G 단백질 알파 서브유닛의 알파-헬리컬 도메인의 실시간 구조 변화 확인 방법 및 시스템

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1532446B1 (en) * 2002-04-12 2012-02-15 Schering Corporation G-protein coupled receptor ligands and methods
US20050042720A1 (en) 2003-04-24 2005-02-24 7Tm Pharma A/S Novel promiscuous G alpha protein mutants and their use
US7488583B2 (en) 2003-09-25 2009-02-10 Odyssey Thera, Inc. Fragment complementation assays for G-protein-coupled receptors and their signaling pathways
WO2005121755A1 (en) 2004-06-14 2005-12-22 Commonwealth Scientific And Industrial Research Organisation Cell free g-protein coupled receptor and ligand assay
GB0822259D0 (en) * 2008-12-05 2009-01-14 Ge Healthcare Uk Ltd Methods for testing binding of a ligand to a G protein- coupled receptor
EP2382470B1 (en) * 2009-01-29 2014-12-10 Commonwealth Scientific and Industrial Research Organisation Measuring g protein coupled receptor activation
GB0905419D0 (en) 2009-03-30 2009-05-13 Ge Healthcare Uk Ltd Methods for testing ligand binding to G protein-coupled receptors
EP2639859A4 (en) 2010-11-11 2015-02-18 Ube Industries CONTAINER FOR A NON-AQUEOUS ELECTROLYTE SOLUTION, NON-ACID ELECTROLYTE SOLUTION FOR FILLING INTO THIS CONTAINER, AND METHOD FOR STORING NON-WATER ELECTROLYTE SOLUTION
US20140031525A1 (en) * 2011-01-21 2014-01-30 Heptares Therapeutics Limited Mutant g-protein coupled receptor proteins and methods for producing them
WO2015095973A1 (en) 2013-12-23 2015-07-02 Université de Montréal Systems and methods for the monitoring of protein complex formation in cells

Also Published As

Publication number Publication date
EP3207052A1 (en) 2017-08-23
WO2016058094A1 (en) 2016-04-21
ES2832331T3 (es) 2021-06-10
IL250949B (en) 2021-07-29
AU2015333543A1 (en) 2017-03-23
CN107074926A (zh) 2017-08-18
EP3207052B1 (en) 2020-09-30
US10877036B2 (en) 2020-12-29
SG11201701895VA (en) 2017-04-27
JP2017536104A (ja) 2017-12-07
EP3207052A4 (en) 2018-04-25
CA2963579A1 (en) 2016-04-21
NZ729735A (en) 2023-05-26
CN107074926B (zh) 2021-09-21
PL3207052T3 (pl) 2021-02-08
IL250949A0 (en) 2017-04-30
AU2015333543B2 (en) 2021-03-04
CA2963579C (en) 2023-10-31
US20170234870A1 (en) 2017-08-17
DK3207052T3 (da) 2020-11-16

Similar Documents

Publication Publication Date Title
JP6932636B2 (ja) Ｇタンパク質活性化を監視するためのＧβγ相互作用タンパク質に基づいたバイオセンサー
Donthamsetti et al. Using Bioluminescence Resonance Energy Transfer (BRET) to characterize agonist‐induced arrestin recruitment to modified and unmodified G protein‐coupled receptors
JP4955391B2 (ja) Ｇタンパク質共役受容体およびそのシグナル伝達経路の断片相補アッセイ
JP6708633B2 (ja) 細胞における生体分子の局在化及び輸送をモニタリングするためのバイオセンサー
US20090298162A1 (en) Biosensors for monitoring receptor-mediated g-protein activation
US10392649B2 (en) Biosensors that detect NAD+
JP5553326B2 (ja) 一分子型プローブ及びその利用
CA2866097C (en) Membrane span-kinase fusion protein and the uses thereof
US20110244487A1 (en) Methods for testing binding of a ligand to a g protein-coupled receptor
US8211655B2 (en) Wild-type receptor assays
Wintgens et al. Characterizing dynamic protein–protein interactions using the genetically encoded split biosensor assay technique split TEV
EP2329268B1 (en) Methods and compounds for testing binding of a ligand to a g protein-coupled receptor
US20220326229A1 (en) Assay for real-time simultaneous recruitment of arrestin isoforms to g protein-coupled receptors
Alvarez-Curto et al. Defining the functional equivalence of wild-type and chemically engineered G protein-coupled receptors
US20120021440A1 (en) Methods for testing ligand binding to g protein-coupled receptors
HK1241023A1 (en) Biosensors for monitoring biomolecule localization and trafficking in cells
HK1241023B (en) Biosensors for monitoring biomolecule localization and trafficking in cells
CA2775278A1 (en) Arrestin biosensor

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180904

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20180904

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191029

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200129

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200714

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200924

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210302

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210407

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20210803

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20210818

R150 Certificate of patent or registration of utility model

Ref document number: 6932636

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250