CA2775278A1 - Arrestin biosensor - Google Patents

Abstract

Described herein is a biosensor, as well as methods and uses thereof. A
resonance energy transfer (RET) biosensor comprising a beta(.beta.)-arrestin tagged with a first and a second chromophore, wherein said first chromophore is a fluorophore and said second chromophore is a fluorophore or a bioluminophore is described.

Description

ARRESTIN BIOSEN R
TECHNICAL FIELD

[0001] Described herein is a novel biosensor and method suitable for monitoring activation of receptors and signaling molecules. More specifically, the use of a modified arrestin as a biosensor to monitor the activation state of receptors is described, such as G
protein-coupled receptors (GPCR). Advantageously, the biosensor and method allow for a highly sensitive and quantitative assay that can be used in large-scale screening analyses.

BACKGROUND

[0002] The largest class of cell surface receptors in mammalian genomes is the superfamily of G protein-coupled receptors (GPCRs). GPCRs are proteins that span the membrane of a cell and relay the information provided by numerous Iigands, e.g. hormones and neurotransmitters, into intracellular signalling pathways. GPCRs are thus the targets of many clinically important drugs, with approximately half of all current prescription drugs acting through GPCRs (Drews J (1996) Genomic sciences and the medicine of tomorrow.
Nat Biotechnol 14: 1516-1518). Examples of GPCRs are many and include beta-2 adrenergic receptor (a2-AR), Frizzled 4 (Fz4), V2-vasopressin receptor (V2R), V1a vasopressin receptor (VIaR), 6-opioid receptor (6-OR), platelet-activating factor receptor (PAFR), CC chemokine receptor type 5 (CCR5), and angiotensin receptor type 1a (AT1aR).

[0003] GRCRs relay the information encoded by the ligand (e.g. hormones and neurotransmitters) through the activation of G proteins and intracellular effector molecules. G
proteins are heterotrimeric proteins, consisting of an alpha, a beta, and a gamma subunit.
The three G-subunits are non-covalently bound together and the G protein as a whole binds to the inside surface of the cell membrane and associates with the GPCR.
Starting in such conformation, the G-alpha subunit is complexed to GDP (guanosine diphosphate).
When a ligand binds to a domain of the GPCR accessible from the outside of the cell membrane, a conformational change in the GPCR occurs, which in turn prompts the exchange of the GDP
for a molecule of guanosine triphosphoate (GTP) on the G-alpha subunit, and activates the G-protein. The G-protein's a subunit, together with the bound GTP, can then dissociate from the R and y subunits to further affect intracellular signaling proteins or target functional proteins directly, depending on the a subunit type (e.g. Gas, Gal/o, Gaq/1 1, Gal 2/13).

[0004] In order to turn off this response by GPCRs to stimulus, or adapt to a persistent stimulus, the activated GPCRs are inactivated. This inactivation may be achieved, in part, by the binding of a soluble protein, R-arrestin (Q-arr), which uncouples the receptor from the downstream G protein after the receptor is phosphorylated by a G protein-coupled receptor kinase (GRK). More specifically, through their binding to agonist-occupied, GRK-phosphorylated receptors, 13-arrs prevent further coupling to G proteins and promote GPCR
endocytosis, thus leading to decreased signalling efficacy.

[0005] Despite our growing understanding of the diversity in GPCR signaling mechanisms, drug efficacy is often defined only in terms of the regulation of the classical G protein signaling. Within this framework, agonists are defined as drugs that stabilize an active receptor conformation that induces G protein activation, whereas inverse agonists favor an inactive receptor state that reduces spontaneous G protein signaling. The question arises as to whether this paradigm may be transferred to drug effects generated through the formation of metastable complexes involving scaffolding proteins such as 3-arr. Because all studies describing R-arr-mediated MAPK signalling have concentrated on agonist drugs, little is known of how ligands that are commonly classified as inverse agonists may regulate the scaffold assembly that is crucial for such signalling.

[0006] In one study (Azzi et al, 2003), this question was addressed by assessing whether {32-adrenergic receptor ((32AR) and V2 vasopressin receptor (V2R) ligands with proven inverse efficacy on adenylyl cyclase (AC) activity could also regulate MAPK
activation via receptor-mediated scaffold formation. It was found that, despite being inverse agonists in the AC pathway, the R2AR (IC1118551 and propranolol) and V2R (SR121463A) induced the recruitment of [3-arr leading to the activation of the ERK cascade. Such observations indicate that the same drug acting on a unique receptor can have opposite efficacies depending on the signaling pathway considered.

[0007] The above study relied on the use of a bimolecular bioluminescence resonance energy transfer (BRET) assay. It was used to assess f -arrestin recruitment to (32AR or V2R.
Fusion proteins consisting of GFP10 variant (GFP) covalently attached to the carboxyl tail of the receptor of interest ([32AR-GFP; V2R-GFP) were co-expressed with [3-arrestin 2 fused at its carboxyl terminus to Rluc (R-arrestin-Rluc). After incubation of the transfected cells with different ligands, coelenterazine 400a (Perkin-Elmer, Wellesley, MA, USA) was added and readings were collected using a modified top-count apparatus (BRETCount, Packard) that allows the sequential integration of the signals detected at 370-450 nm and 500-530 nm. The BRET signal was determined by calculating the ratio of the light emitted by the Receptor-GFP (500-530 nm) over the light emitted by the 0-arrestin2-Rluc (370-450 nm).
The values were corrected by subtracting the background signal detected when the 0-arrestin2-Rluc construct was expressed alone.

[0008] While the results elicited from the above study were instructive, a necessary feature involved the construction of fusion proteins that included the receptors of interest. Ideally, a method could be devised in which receptor activation might be observed without first having to modify the receptors that are to be studied. Other features of such a method that would make it highly desirable for research and development endeavors include the following: (1) a high level of sensitivity; (2) an ability to provide quantitative results; (3) adaptability for use in large scale screening analyses; (4) an assay that requires the expression of a single recombinant construct; and (5) a biosensor based on an intramolecular RET
signal.

[0009] Resonance energy transfer (abbreviated RET, and also referred to as Forster resonance energy transfer), is a mechanism describing energy transfer between two chromophores, having overlapping emission/absoprtion spectra. When the two chromophores (the "donor" and the "acceptor"), are within 10-100 A of one another and their transition dipoles are appropriately oriented, the donor chromophore is able to transfer its excited-state energy to the acceptor chromophore through nonradiative dipole-dipole coupling. When both chromophores are fluorescent, the term typically used is "fluorescence resonance energy transfer" (abbreviated FRET). In bioluminescence resonance energy transfer (BRET), the donor chromophore of the RET pair, rather than being a fluorophore, is a bioluminescent molecule, typically luciferase. In the presence of a substrate, bioluminescence from the donor excites the acceptor fluorophore through the same Forster resonance energy transfer mechanism described above (Xu, Y. et al., PNAS, 96:151-156 (1999)).

[0010] There is a need for a simpler method to measure receptor activity in living cells. The present invention seeks to meet this and related needs.

SUMMARY

[0011] A resonance energy transfer (RET) biosensor comprising an arrestin tagged with a first and a second chromophore, wherein said first chromophore is a fluorophore and said second chromophore is a fluorophore or a bioluminophore.

[0012] Further, there is described herein a method of identifying candidate molecules that bind to a receptor comprising screening candidate molecules for activation of the biosensor.
Uses of the biosensor for assaying receptor activity are described, as well as kits for evaluating receptor binding.

[0013] Other objects, advantages and features of the present invention will become more apparent upon reading of the following non-restrictive description of preferred embodiments thereof, given by way of example only with reference to the accompanying figures.

BRIEF DESCRIPTION OF THE FIGURES

[0014] FIGURE 1: Double-brilliance A-arr. Schematic diagram illustrating how agonist-promoted conformational rearrangement of R-arr can be measured as changes in BRET
using double-brilliance [3-arr. Luc and YFP are represented by cylinders proportional to their sizes, but their real orientation is unknown.

[0015] FIGURE 2: Functionality of double-brilliance R-arr. HEK293 (A-C) or COS
(D) cells were transiently transfected with the indicated plasmids. (A) Cells incubated or not In the presence of saturating concentrations of specific agonists (02-AR, 10 mM
isoproterenol (ISO); V2R, 1 mM arginine vasopressin (AVP)). Localization of Luc-P-arr-YFP
and Myc-tagged receptors was analysed by confocal fluorescence microscopy. (B) Agonist-induced recruitment of R-arr measured using intermolecular BRET2. tl/2=half-time of maximal R-arr recruitment. (C) Dose-dependent recruitment of R-arr to the receptors measured in intermolecular BRET2 following 2 min stimulation with the agonist.
EC50=concentration of agonist producing half-maximal 13-arr recruitment. (D) Cells treated or not for 15 min with the specific agonists at 37 C and cell-surface receptor levels measured by enzyme-linked immunosorbent assay (ELISA). Receptor endocytosis is defined as the loss of cell-surface immunoreactivity and is expressed as a percentage of total immunoreactivity measured under basal conditions. Expression levels of (3-arr were controlled using western blot (data not shown). Data are the mean t s.e.m. of at least three independent experiments. *P<0.05 between treatment and each individual control condition. Mock, non-transfected cells.

[0016] FIGURE 3: AVP-induced conformational change of R-arr monitored by intramolecular BRET1. HEK293 cells were transfected with the indicated plasmids and BRET was measured at 25 C in the presence of coelenterazine h. (A) Specificity of agonist-induced G-arr intramolecular BRET1. (B) Real-time BRET measurements of the agonist-induced R-arr conformational change. tl/2=half-time of maximal conformational change of R-arr. (C) Dose-dependent agonist-promoted increase of [3-arr intramolecular BRET1. Cells were stimulated with increasing concentrations of AVP for 4 min. EC50=concentration of AVP
producing half-maximal conformational change of R-arr. Data are the ' mean t s.e.m. of at least three independent experiments. *P<0.01 between treated and control condition.

[0017] FIGURE 4: Agonist-promoted conformational change of a phosphate insensitive Rarrestin mutant. HEK293 cells were transfected with V2R and either Luc-Barr-YFP or Luc-(3arr(R169E)-YFP. Cells were stimulated or not for 10 min with 1 pM AVP prior the addition of 5 pM coelenterazine h (Molecular Probe) and performing the intramolecular measurements using a Multilabel Reader Mithras LB 940 (Berthold Technologies).
The BRET signal was determined by calculating the ratio of the light emitted by YFP over the light emitted by Luc following the addition of coelenterazine h. The values were corrected by subtracting the background BRET signals detected when Luc-Parr was expressed alone.
Inset, AVP-induced BRET increase. Data represent the mean t SEM of three independent experiments. * indicates p<0.02 between treatment and each individual control condition.

[0018] FIGURE 5: Double-brilliance Q-arr monitors the activation of many GPCRs. HEK293 cells were transfected with Luc-f3-ar -YFP and either pcDNA3.1 or plasmids encoding the indicated receptors. (A) Agonist-Induced translocation of Luc-(3-arr-YFP
measured following treatment with 1 mM of the specific agonists (f32-AR, ISO; V1aR, AVP; 5-OR, SNC80; PAFR, PAF; CCR5, hRANTES; AT1aR, angiotensin II). (B) Agonist-induced conformational change of Luc-R-arr-YFP measured following 10 min stimulation with the specific agonists mentioned in (A). BRET1 was measured using a Multilabel Reader Mithras LB 940 (Berthold Technologies). The BRET signal was determined by calculating the ratio of the light emitted by YFP over the light emitted by Luc following the addition of coelenterazine h. Data are the mean s.e.m. of three independent experiments. *P<0.05 between treatment and each individual control condition.

[0019] FIGURE 6: Agonist-promoted conformational change of constitutively activated parrestin mutants. HEK293 cells were transfected with V2R and either Luc-parr-YFP or Luc-(3arr (3A)-YFP or Luc-Parr (IV)-YFP. Cells were stimulated or not for 10 min with 1 pM AVP
prior to the addition of 5 pM coelenterazine h and performing the BRET
measurements as described in the previous figure. Inset, AVP-induced BRET increase. The BRET
signal was determined by calculating the ratio of the light emitted by YFP over the light emitted by Luc following the addition of coelenterazine h. The values were corrected by subtracting the background BRET signals detected when Luc-Parr was expressed alone. Data represent the mean SEM of two independent experiments. * Indicates p<0.05 between treatment and each individual control condition.

[0020] FIGURE 7: Conformational change of parrestin induced by ligands of different efficacies. HEK293 cells transiently co-expressing the V2R and Luc-Parr-YFP
were subjected to real-time BRET measurements in the presence or absence of two different V2R
ligands.
The basal BRET signals were subtracted from each condition to express the data as ligand-induced BRET increase. The figure shows the detection of conformational changes of Luc-parr-YFP in time, reflected by the increase in BRET signal, as Induced by the V2R agonist AVP or the inverse agonist SR121463. No BRET increase was observed when cells were incubated in the presence of the carrier alone (non-stimulated). The fact that the observed increase in BRET signal induced by SR121463 is significantly lower than that induced by AVP treatment can be correlated with the smaller SR121463-mediated recruitment of parrestin to the V2R when compared to AVP, as reported previously (Azzi et at, 2003).

[0021] FIGURE 8: parrestin-dependant endocytosis beyond GPCRs. (A) Endocytosis of the receptor Frizzled 4 (Fz4) stimulated by Wnt5a is orchestrated by parrestin 2, in a manner that is dependent upon the phosphorylation of the adaptor protein Dishevelled 2 (Dvl2) by protein kinase C (PKC). (B) Endocytosis of the RII and Rill receptor subtypes of TGF-01 is orchestrated by parrestin 2, and facilitated by the phosphorylation of Rill by Ril. (C) Endocytosis of the IGF1 receptor is orchestrated by parrestin (Modified from Lefkowitz &
Whalen, 2004.).

[00221 FIGURE 9: Characterization of BRET2-RArrestin double-brilliance sensors. (A) Structure and activation: BRET1 and BRET2-[3Arrestin double brilliance (db) sensors are unimolecular with BRET tags in N-and C-terminus of a central RArrestin core.
The linkers separating the BRET1 and BRET2 tags from RArrestin differ in both length and composition.
For the BRET1 sensor the structure is: BRET donor (Rluc)-Linkerl- [3Arrestin-Linker2-BRETI
acceptor (YFP) and for the BRET2 sensors: Structure: BRET2 acceptor (sCFP3A, mAmetrine or GFP10)-Linker3- RArrestin- Linker4- BRET2 donor (Rlucll). For BRETI, the Rluc substrate is coelenterazine H, whereas for BRET2, the Rluc substrate is deep-blue coelentrazine. All versions of the (iArrestinl and 2 db are conformational sensors. However, following GPCR activation by an agonist (illustrated as a triangle), changes in RArrestin conformation lead to a decreased BRET signal for the BRET2 sensors while it leads to an increased BRET signal with the BRET1 sensor configuration (see Figures 1-7).
(B) Kinetics and dose-responses measured in BRET2 with the BRET2-(3ARR1 and 2 db sensors, in response to V2R activation by its agonist AVP: at 100nM for the kinetics or at increasing concentrations of AVP for dose-response experiments. (C) PARR db sensor to characterize ligands of different efficacies. Hek293 cells transiently expressing both AT1aR and GFP10-{3arrl-Rlucil db sensor, were stimulated with a full (Angll) or partial agonists and responses were evaluated as a BRET2 signal modulation. a) Dose-dependent ligand-promoted decrease of (3arrestin intramolecular BRET2 signal after a 25 min stimulation.
Data are the mean +/- S.E.M. of 3 independent experiments. b) Agonist-promoted BRET
changes. Cells were treated for 25 min with 1 pM Angil or 10pM of the partial agonists. Data represent mean +/- S.E.M. of 4 independent experiments. One-way ANOVA followed by Tukey's multiple comparison post-hoc test (Angll as reference) was used to assess statistical significance. *, p < 0.05, """*, p < 0.001. Angll= Angiotensin 2 octapeptide, SVdF: Angll analog with Sar,,Val5,D-Phe8 substitutions at the indicated amino acid positions in the octapeptide, SII:
Angll analog with Sar,,lle4ille8 substitutions at the indicated amino acid positions in the octapeptide, SBpA: Angll analog with Sar,,Bpa8 substitutions at the indicated amino acid positions in the octapeptide, SIVI: Angli analog with Sar,,lle8 substitutions at the indicated amino acid positions in the octapeptide, DVG: Angli analog with Asp,,Val5,Gly8 substitutions at the indicated amino acid positions in the octapeptide.

[0023] FIGURE 10: Z'-factor evaluation for both BRETI- and BRET2-[3Arrestin sensors.
HEK293 cells transiently expressing both V2R and the double-brilliance sensor, were exposed to either 100nM AVP or a control vehicle, for 25-35 min. BRET ratios are represented for each individual well of a 96-well plate. Z'-factors were calculated as described in (Ji-Hu Zhang et al. 1999 J Biomol Screen, 4; 67). A Z'-factor between 0.4 and 1 is considered a robust assay.

DETAILED DESCRIPTION

[0024] A resonance energy transfer (RET) biosensor is described herein which comprises an arrestin tagged with a first and a second chromophore. The first chromophore is a fluorophore and said second chromophore is a fluorophore or a bioluminophore.
For example, the arrestin may be 0-arrestin-1 (arrestin-2), 0-arrestin-2 (arrestin-3), arrestin-1 or arrestin-4.

[0025] The second chromophore may be a bioluminophore and the RET is bioluminescence resonance energy transfer (BRET), in certain embodiments. An exemplary bioluminophore may be Renilla luciferase or a mutant form of Renilla luciferase. A specific bioluminophore could be Riucil (A55T/C124A/M185V).

[0026] The fluorophore may be a fluorescent protein, for example green fluorescent protein or a variant thereof. Specifically, the fluorophore could be YFP, mAmetrine, cyan fluorescent protein (CFP), or GFP10.

[0027] Specific exemplary biosensors may comprise Luc-R-arr--YFP, YFP-Barr-Luc, Luc-0-arr(3A)-YFP, Luc-R-arr(IV)-YFP, Luc-J3arr(R169E)-YFP, Ametrine-hpar1-Rlucil, Ametrine-hRarr2-RluciI, CFP-hlarrl-Rlucil, CFP-h[3arr2-Rlucil, GFPI0-h[3arrl-Riucli, or h(3arr2-Rlucil.

[0028] In certain embodiments, the fluorophore may be positioned at the N-terminus of the arrestin and the bioluminophore at the C-terminus of the arrestin. Further, in such an embodiment, the fluorophore can be green fluorescent protein or a variant thereof.
Specifically, the fluorophore might be YFP, mAmetrine, cyan fluorescent protein (CFP), or GFP10. The bioluminophore, in certain embodiments, can be Renilla luciferase or a mutant form thereof, for example Rlucl I (A55T/C124A/M 185V).

[0029] The biosensor may have a fluorophore at the C-terminus of the arrestin and the bioluminophore at the N-terminus of the arrestin. In this case, the fluorophore may be green fluorescent protein or a variant thereof, such as YFP, mAmetrine, cyan fluorescent protein (CFP), or GFP10. The bioluminophore of such an embodiment may be Renilla luciferase or a mutant form thereof.

[0030] In certain embodiments of the biosensor, one of the fluorophore or bloluminophore may be linked to a position between the C and N-termini. For example, the bioluminophore may be linked to the C-terminus of the arrestin while the fluorophore may be linked internally to the arrestin. In such exemplary embodiments, the fluorophore may be YFP, mAmetrine, cyan fluorescent protein (CFP), or GFP10, and the bioluminophore could be Renilla luciferase or a mutant form thereof.

[0031] In certain embodiments of the biosensor, both said first and said second chromophores may be fluorophores and the RET may be fluorescence resonance energy transfer (FRET). For example, the fluorophore donor can be CFP or a variant thereof, while the the fluorophore acceptor could be a YFP or a variant thereof. YFP may be a non-circularly permuted sYFP2 or a circularly permuted sYFP2.

[0032] A method of identifying candidate molecules is described herein, which that bind to a receptor. The method may comprise (a) screening candidate molecules for activation of a biosensor in which the second chromophore Is a bioluminophore and the RET is bioluminescence resonance energy transfer (BRET), and in which the fluorophore is at the C-terminus of the arrestin and the bioluminophore Is at the N-terminus of the arrestin, so as to determine a candidate population; and (b) screening this candidate population for activation using a further biosensor to identify candidate molecules that bind to receptors. In this case, the further biosensor used to screen the candidate population determined in (a) is one in which the second chromophore is a bioluminophore and the RET is bioluminescence resonance energy transfer (BRET), and in which the fluorophore is at the N-terminus of the arrestin and the bioluminophore is at the C-terminus of the arrestin.

[0033] In the above method, the receptor may be a Frizzled protein receptor or a G protein-coupled receptor (GPCR). An exemplary receptor may be Frizzled 4(Fz4), R2AR, VI
vasopressin receptor (V1aR), V2 vasopressin repressor (V2R), delta opioid receptor, platelet-activating factor receptor, CC chemokine receptor type 5, or angiotensin receptor type 1 a.

[0034] In the method described above, the activation of the biosensor in (a) may be observed by an increase in BRET signal. The activation of the biosensor in (b) may result in a decrease in BRET signal. The candidate molecule that binds the receptor may be an agonist, inverse agonist, partial agonist, antagonist or an allosteric regulator.

[0035] The above-described biosensor may used for assaying receptor activity.
Further, the biosensors as described in the above method, in parts (a) and (b), may be used together for identifying agonists, inverse agonists, partial agonists, antagonists or allosteric regulators.
[0036] A kit is described herein for evaluating receptor binding. The kit may comprise the biosensor described in part (a) of the method described above, together with the biosensor described in part (b) of the method above. Such a kit may be used for evaluating receptor binding to identify candidate molecules that bind to the receptor, and thus may include instructions for use in the above.

[0037] In the method described above, the way in which molecules can be screened may comprise identifying an agonist or inverse agonist for the receptor by incubating (i) cells co-expressing the receptor and the biosensor with a potential agonist or inverse agonist, or (ii) an isolated receptor and the biosensor with a potential agonist or inverse agonist. Following this, a suitable substrate may be added to detect bioluminescence resonance energy transfer (BRET) in said biosensor. The BRET signal can then be detected and compared with a BRET signal obtained under similar conditions in the absence of the potential agonist or inverse agonist. The potential agonist or inverse agonist may thus be identified as an agonist or inverse agonist if a change in BRET signal level is observed.

[0038] In this method, the BRET signal may be evaluated by detecting light emissions at about 440-510 nm and at about 510-570 nm. As a further option, the BRET signal may be evaluated by detecting light emissions at about 320-490 nm and at about 490-550 nm. The receptor may be a Frizzled protein receptor or a G protein-coupled receptor.
Further, receptor may be chosen from the exemplary group of: Frizzled 4 (Fz4), 02AR, V1 vasopressin receptor (V1 aR), V2 vasopressin receptor (V2R), delta-opioid receptor (SOR), platelet-activating factor receptor (PAFR), CC chemokine receptor type 5 (CCR5), and angiotensin receptor type Ia (AT1aR).

DEFINITIONS:
[0039] Unless otherwise defined, the terms used in the present description have the meanings that would be understood by a person of skill in the art.

[0040] Ligand: A molecule which may be but is not restricted to a hormone, neurotransmitor, chemical compound, drug, or diagnostic agent that binds to a receptor and has an agonistic, inverse agonistic, antagonistic or allosteric effect on the receptor. Ligands may be further classified as follows (for a more detailed summary, see Wilson, Keith et al.
(Eds.), Principles and Techniques of Biochemistry and Molecular Biology, 7th Edition (2010), Chapter 17, incorporated by reference herein):

a) Agonist: a ligand that has the same or similar effect as a hormone, neurotransmitter or signaling molecule or a group of hormones, neurotransmitters or signaling molecules activating a receptor, by binding to the same natural receptor. A
partial agonist is a type of agonist that with lower intrinsic activity than a full agonist and that produces a lower maximum effect. Examples of agonists include:

i. Angiotensin II: The active form of angiotensin. An octapeptide found in blood, it is synthesised from angiotensin I and quickly destroyed. Angiotensin II causes profound vasoconstriction with resulting increase in blood pressure. It is an agonist of the angiotensin receptor.

ii. AVP: arginine vasopressin, vasopressin containing arginine, as that from most mammals, including man. This hormone controls water reabsorbtion by the kidney and is also known as the antidiuretic hormone.

iii. ISO: isoproterenol, a synthetic beta-adrenergic receptor agonist which controls peripheral vasoconstriction, bronchodilation and increased cardiac rate, contractility and output.

iv. SNC80: 4-[( R)-a-((2S,5R)-4-allyl-2,5-dimethyi-1-piperazinyl)-3-methoxybenzyl]-N,N-diethylbenzamide. An agonist of the delta-opioid receptor that possesses anti-nociceptive action.

v. PAF: platelet-activating factor; a hormone that regulates platelet aggregation. It is an agonist of the PAF receptor.

vi. hRANTES: human RANTES (regulated upon activation, normal T cell expressed and secreted) is a chemoattractant for monocytes and T cells. It is an agonist of the chemokine receptors: CCR1, CCR3, CCR5 and GPR75.

vii. Wnt5a: Ligand for members of the frizzled family of seven transmembrane receptors.

viii.IGF1: insulin-like growth factor 1 (also known as somatomedin C), a hormone homologous to proinsulin.

ix. TGF-01: Transforming Growth Factor-beta1, a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta 1 and essentially all of them have specific receptors for this peptide. TGF-beta 1 regulates the actions of many other peptidic growth factors and determines a positive or negative direction of their effects.

b) Inverse agonist: a ligand that produces an effect opposite to that of an agonist by occupying the same receptor. Examples include:

i. SR121463: SR121463 is a selective, orally active, non-peptide antagonist of vasopressin (AVP) V2 receptors, with powerful aquaretic, properties in various animal species and humans. SR121463 also behaves as an inverse agonist in cells expressing constitutively active human V2 receptor.

c) Antagonist: a ligand that counteracts the effect of another ligand (agonist or inverse agonist) acting on a receptor by binding to the same receptor, thus blocking or dampening the ability of the agonist to bind (also called competitive antagonist).
Neutral antagonists have affinity but no efficacy for their cognate receptors.

d) Allosteric regulator: a ligand that modulates receptor activity through binding at a site that is different from that bound by orthosteric ligands (i.e. endogenous ligands).
Allosteric regulators may have an antagonistic or agonistic effect.

[0041] Chromophore: A small molecule, or a part of a larger molecule, that is responsible for the spectral band of the molecule.

[0042] Blosensor: A type of biomolecular probe that measures the presence or concentration of biological molecules, biological structures, activity state etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal such as light or electric pulse.

[0043] Receptor: A popular and generally accepted hypothesis that appears to explain many pharmacodynamic phenomena holds that specialized protein molecules on the surfaces of cells provide a "fit" for an intrinsic molecule (such as a hormone or neurotransmitter) or a drug such that when that molecule occupies (binds to) that area, it leads to a biochemical or physiologic response. This Idea is often compared to the operation of a lock (receptor) by a key (Iigand). Examples of GPCR receptors include:

a) 12-AR: beta-2 adrenergic receptor b) Frizzled 4 (Fz4): a seven transmembrane receptor that selectively recognizes hormones of the Wnt family.

C) V2R: Vasopressin V2 receptor d) VIaR: Vasopressin V1a receptor e) 5-OR: b-opioid receptor f) PAFR: platelet-activating factor receptor g) CCR5: CC chemokine receptor type 5 h) AT1aR: angiotensin receptor type 1a (0044] Signalling molecule: a membrane or soluble protein involved in the transaction of signals in cells initiated by hormones, neurotransmitters or synthetic ligands.

[0045] Identity as known in the art, is a relationship between two or more polypeptide sequences, as determined by comparing the sequences. In the art, "identity"
also means the degree of sequence relatedness between polypeptides as determined by the match between strings of such sequences. "Identity" and "similarity" can be readily calculated by known methods, including, but not limited to, those described in (Computational Molecular Biology, Lesk, A. M., Ed., Oxford University Press, New York, 1988; Biocomputing:
Informatics and Genome Projects, Smith, D. W., Ed., Academic Press, New York, 1993; Computer Analysis of Sequence Data, Part I, Griffin, A. M., and Griffin, H. G., Eds., Humana Press, New Jersey, 1994; Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987; and Sequence Analysis Primer, Gribskov, M. and Devereux, J., Eds., M Stockton Press, New York, 1991; and Carillo, H., and Lipman, D., SIAM J Applied Math., 48: 1073 (1988).

[0046] By way of example, a polypeptide sequence may be identical to the reference sequence, that is be 100% identical, or it may Include up to a certain integer number of amino acid residue alterations as compared to the reference sequence such that the %
identity is less than 100%. Such alterations are selected from: at least one conservative or non-conservative amino acid residue substitution, deletion, or insertion, and wherein said alterations may occur at the amino- or carboxy-terminal positions of the reference polypeptide sequence or anywhere between those terminal positions, interspersed either individually among the amino acid residues in the reference sequence or in one or more contiguous groups within the reference sequence. The number of amino acid residue alterations for a given % identity is determined by multiplying the total number of amino acids in the reference polypeptide by the numerical percent of the respective percent identity (divided by 100) and then subtracting that product from said total number of amino acids in the reference polypeptide.

[0047] Conservative amino acid variants can also comprise non-naturally occurring amino acid residues. Non-naturally occurring amino acids include, without limitation, trans-3-methylproline, 2,4-methanoproline, cis-4-hydroxyproline, trans-4-hydroxyproline, N-methyl-glycine, allothreonine, methylthreonine, hydroxy-ethylcysteine, hydroxyethylhomocysteine, nitro-glutamine, homoglutamine, pipecolic acid, thiazolidine carboxylic acid, dehydroproline, 3- and 4-methyiproline, 3,3-dimethylproline, tert-leucine, norvaiine, 2-azaphenyl-alanine, 3-azaphenylalanine, 4-azaphenylalanine, and 4-fluorophenylalanine. Several methods are known in the art for incorporating non-naturally occurring amino acid residues into proteins.
For example, an in vitro system can be employed wherein nonsense mutations are suppressed using chemically aminoacylated suppressor tRNAs. Methods for synthesizing amino acids and aminoacylating tRNA are known in the art. Transcription and translation of plasmids containing nonsense mutations is carried out in a cell-free system comprising an E.
coli S30 extract and commercially available enzymes and other reagents.
Proteins are purified by chromatography. (Robertson, etal., J. Am. Chem. Soc, 113:
2722,1991; Ellman, et a!., Methods Enzymoi, 202: 301, 1991; Chung, etal., Science, 259: 806-9, 1993;
and Chung, et a!, Proc. Nati. Acad. Sci. USA, 90: 10145-9, 1993). In a second method, translation is carried out in Xenopus oocytes by microinjection of mutated mRNA and chemically aminoacylated suppressor tRNAs (Turcatti, at a!, J. Biol. Chem., 271: 19991-8, 1996). Within a third method, E. coli cells are cultured in the absence of a natural amino acid that is to be replaced (e.g., phenylalanine) and in the presence of the desired non-naturally occurring amino acid(s) (e.g., 2-azaphenylalanine, 3-azaphenylalanine, 4-azaphenylalanine, or 4-fluorophenylalanine). The non-naturally occurring amino acid is incorporated into the protein in place of its natural counterpart. (Koide, et al, Biochem., 33: 7470-6, 1994). Naturally occurring amino acid residues can be converted to non-naturally occurring species by in vitro chemical modification. Chemical modification can be combined with site-directed mutagenesis to further expand the range of substitutions (Wynn, et a!. Protein Sci., 2: 395-403, 1993).

[0048] Variant: refers to a polypeptide or polynucleotide that differs from a reference polypeptide or polynucleotide, but retains essential properties. A typical variant of a polypeptide differs in amino acid sequence from another, reference polypeptide. Generally, differences are limited so that the sequences of the reference polypeptide and the variant are closely similar overall and, in many regions, identical. A variant and reference polypeptide may differ in amino acid sequence by one or more modifications (e.g., substitutions, additions, and/or deletions). A variant of a polypeptide includes conservatively modified variants. A substituted or inserted amino acid residue may or may not be one encoded by the genetic code. A variant of a polypeptide may be naturally occurring, such as an allelic variant, or it may be a variant that is not known to occur naturally.

[0049] Modifications and changes can be made in the structure of the polypeptides of this disclosure and still obtain a molecule having similar characteristics as the polypeptide (e.g., a conservative amino acid substitution). For example, certain amino acids can be substituted for other amino acids in a sequence without appreciable loss of activity. Because it is the interactive capacity and nature of a polypeptide that defines that polypeptide's biological functional activity, certain amino acid residue substitutions can be made in a polypeptide sequence and nevertheless obtain a polypeptide with like properties.

[0050] In one aspect, such variants have at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 91, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity with the reference polypeptide or polynucleotide.

ARRESTIN:
[0051] A receptor could either be constitutively active or inactive and, ligands such agonists, inverse agonists and allosteric modulators are known to modulate this activity. The interaction of arrestins, including a-arrestin (Garr), with receptors, such as but not limited to GPCRs, is a reflection of the receptor activity. The beta-arrestins belong to the family of arrestins. It is generally accepted that there are 4 arrestins in mammals:
arrestins 1-4.
Arrestin-1 and arrestin-4 are visual arrestins whereas arrestin-2 and arrestin-3 are widely distributed in all tissues and correspond to beta-arrestin-1 and beta-arrestin-2 respectively.
The interaction of R-arrestins with receptors or other proteins, such as a beta2-adaptin, has an impact on the conformation of the (3-arrestins. This change in conformation is linked to its property of interacting with effectors of signaling pathways and receptor endocytosis. This characteristic of arrestin is conserved throughout evolution of eukaryotic organisms and, is the basis for the unimolecular RET-based conformational sensors: a-arrestinl (also known as Arrestin-2 or R-arrestin) and R-arrestin 2 (also known as Arrestin-3) double brilliance sensors to monitor receptor activity. A conformational change in 13-arrestin is monitored through a modulation of the RET signal.

[0052] The arrestins exemplified herein are human f3-arrestins (hparr) and mutants and variants thereof; however, other arrestins are contemplated, including proteins having at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 92%, at least 94%, at least 96%, at least 98%, or at least 99% sequence identity with h(3arr1, h3arr2, or with another arrestin, wherein such proteins interact with GPCRs.

RESONANCE ENERGY TRANSFER ASSAYS:

[0053] Resonance energy transfer (abbreviated RET, and also referred to as Forster resonance energy transfer) is a mechanism describing energy transfer between two chromophores, having overlapping emission/absoprtion spectra. When the two chromophores (the "donor" and the "acceptor"), are within 10-100 A (Angstroms) of one another and their transition dipoles are appropriately oriented, the donor chromophore is able to transfer its excited-state energy to the acceptor chromophore through non-radiative dipole-dipole coupling.

[0054] Bioluminescence Resonance Energy Transfer (BRET) Assay is a proximity assay based on the non-radiative transfer of energy between a donor bioluminophore (bioluminescent enzyme (ex: luciferase)) and an acceptor fluorophore (ex: GFP
or YFP).

[0055] As used herein, BRET1 uses coelenterazine h as the luciferase substrate (i.e.
bioluminescent initiator molecule) and YFP and its variants as the energy acceptor. BRET2 uses coelenterazine 400a (Perkin-Elmer, Wellesley, MA, USA and, Biotium Inc, Hayward, CA, USA) as the luciferase substrate and CFP, GFP2, GFP10, Tsapphire or mAmetrine as the energy acceptor. BRET1 and BRET2 represent different variants of BRET that are based on the use of different, luminescent enzymes, luciferase substrates and different fluorescent proteins. The difference between the BRET1 and BRET2 biosensors as used herein also incorporates differences in both the linkers used to join the chromophores to the beta arrestin molecules and the orientation of the chromophores relative to each other (i.e.
to which terminal of beta-arrestin are they linked.

[0056] Each version of BRET typically uses a different coelenterazine to be able to excite the acceptor at different wavelengths. Typically, the acceptor for BRET1 is a YFP
and for BRET3 is an OFP (Abhijit De, Pritha Ray , Andreas Markus Loening and Sanjiv Sam Gambhir, BRET3: a red-shifted bioluminescence resonance energy transfer (BRET)-based integrated platform for imaging protein-protein interactions from single live cells and living animals The FASEB Journal. 23(8): 2702-2709, incorporated by reference herein) For BRET2 the acceptor is typically any fluorophore that can be excited close to 400nM such as BFP, Cyan, GFP or mKeima (RFP).

[0057] (i) bioluminophore: The bioluminophore in the BRET assay is a protein, that catalyzes the reaction of a substrate (i.e. a bioluminescent initiator molecule) producing bioluminescence.

[0058] Luciferase is an example of a protein that catalyzes the oxidation of its substrate (ex:
coelenterazine) producing light, and can be used as a bioluminophore. As used herein, luciferases refer to an enzyme that catalyzes a bioluminescent reaction (a reaction that produces bioluminescence). In representative embodiments, the subject luciferase polypeptides are polypeptide sequences of the Renilla reniformis wild-type and mutant luciferases, which are known and reported in Lorenz et al., Proc. Natl. Acad.
Sci. USA (1991) 88:4438-4442, Loening et al., Protein Eng Des Sel. (2006) 19(9):391-400, and also reported in U.S. Pat. No. 6,451,549 as SEQ ID NOS: 1 and 2, and in U.S. Pat. No.
7,842,469, the disclosure of which is herein incorporated by reference.

[0059] In representative embodiments, the subject luciferase polypeptides may also be mutants (also referred to as variants herein) of wild-type luciferases found in Renilla species (e.g., Renilla koellikeri; Renilla muelleri and Renilla reniformis, where in representative embodiments, the mutant luciferase is a mutant of the Renilla reniformis wild-type luciferase).
The term "mutant" is employed broadly to refer to a protein that differs in some way from a reference wild-type protein, where the subject protein retains at least one biological property of the reference wild-type (e.g., naturally occurring) protein. The term "biological property" of the subject proteins includes, but is not limited to, spectral properties, such as emission maximum, quantum yield, and brightness (e.g., as compared to the wild-type protein or another reference protein such as firefly luciferase from P. pyralis), and the like; in vivo and/or in vitro stability (e.g., half-life); and the like. In particular, the mutants (or variants) retain luciferase activity (e.g., catalyze the conversion of a coelenterazine substrate into a luminescent product in the presence of molecular oxygen). Mutants of the disclosure include single amino acid changes (point mutations), deletions of one or more amino acids (point-deletions), N-terminal truncations, C-terminal truncations, insertions, and the like.

[0060] For purposes of the disclosure, a naturally occurring luciferase is a reference wild type luciferase for a given mutant if the amino acid sequences of the wild-type and the mutant have high identity over at least the length of the mutant (e.g., at least about 90%, at least about 95%, at least about 97%, at least about 98%, at least about 99% or higher) but will not have complete sequence identity in representative embodiments.

[0061] In representative embodiments, the mutants encoded by the subject polynucleotides exhibit increased light output as compared to their corresponding reference wild-type protein.
Specifically, the subject mutants have at least enhanced light output with a given coelenterazine substrate as compared to their corresponding reference wild type. For purposes of the present disclosure, increased light output is determined by evaluating at least one of the kinetics and quantum yield of a given mutant using a convenient assay known to those of skill in the art. In representative embodiments in which the subject polynucleotides encode a mutant of Renilla luciferase that exhibits enhanced light output, the encoded mutant may include a substitution at at least one of the following positions: C124;
K136; M185, and S287. In one aspect the Renilla luciferase mutant has the following substitutions: C124A and M185V. In another aspect the Renilla luciferase mutant has the following substitutions: A55T, C1 24A and M1 85V, and is referred to herein as Rlucll. These mutations and variations thereof are known (see Loening et al., Protein Eng Des Sel. (2006) 19(9):391-400 and US 7,842,469, both of which are incorporated herein), and are contemplated for use herein. Examples of Renilla luciferase proteins contemplated herein include proteins that have an amino acid-sequence selected from:

Rluc WT
MTSKVYDPEQRKRM ITGPQW WARCKQMNVLDS FI NYYDSEKHAENAVI FLHGNAASSYLW
RHWPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWFELLNLPKKI IFVGHDWGA
CLAFHYSYEHQDKIKAIVHAESWDVIESWDEWPDIEEDIALIKSEEGEKMVLENNFFVETML
PSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREIPLVKGGKPDWQIVRNYNAYLRASD
DLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNE
Q

Rlucll (A55T/C124A/M185V) MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENAVIFLHGNATSSYLW
RHWPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWFELLNLPKKIIFVGHDWGA

ALAFHYSYEHQDKIKAIVHAESWDVIESWDEWPDIEEDIALIKSEEGEKMVLENNFFVETVLP
SKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREIPLVKGGKPDWQIVRNYNAYLRASDD
LPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ*
RIuc8 (A55T/C124A/S130A1K136R/A143M/M 185V/M253L/S287L) MASKVYDPEQRKRM ITGPQWWARCKQMNVLDSFINYYDSEKHAENAVI FLHGNATSSYLW
RHWPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWFELLNI,PKKIIFVGHDWGA
ALAFHYAYEHQDRIKAIVHMESWDVIESWDEWPDIEEDIALIKSEEGEKMVLENNFFVETVL
PSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREIPLVKGGKPDWQIVRNYNAYLRASD
DLPKLFIESDPGFFSNAIVEGAKKFPNTEFVKVKGLHFLQEDAPDEMGKYIKSFVERVLKNEQ
portions thereof, mutants thereof, variants thereof, or conservative variants thereof. Other luciferase variants known in the art include those disclosed in US
2009/0136998, incorporated by reference herein.

[0062] In representative embodiments, the mutant luciferase polynucleotides encoded by the nucleic acids are mutants of luciferase polynucleotides that employ a coelenterazine as a substrate, where the term coelenterazine refers collectively to native coelenterazine, as well as analogues thereof, where representative coelenterazine analogues of interest include, but are not limited to: benzyl-coelenterazine; coelenterazine-cp; coelenterazine-n; bis-deoxy-coelenterazine (also known as coelenterazine 400a and DeepBlue-coelenterazine); and the like.

[0063] In addition to the above-described specific subject polynucleotide compositions, also of interest are homologues of the above-sequences. With respect to homologues of the subject polynucleotide, the source of homologous genes may be any species of plant or animal, or the sequence may be wholly or partially synthetic. In certain embodiments, sequence similarity between homologues is at least about 20%, at least about 25%, and may be 30%, 35%, 40%, 50%, 60%, 70% or higher, including 75%, 80%, 85%, 90% and 95% or higher. Sequence similarity is calculated based on a reference sequence, which may be a subset of a larger sequence, such as a conserved motif, coding region, flanking region, and the like. A reference sequence will usually be at least about 18 nt long, more usually at least about 30 nt long, and may extend to the complete sequence that is being compared.
Algorithms for sequence analysis are known in the art, such as BLAST, described in Altschul et al. (1990), J. Mol. Biol. 215:403-10 (using default settings, e.g.
parameters w=4 and T=17). The sequences provided herein are used for recognizing related and homologous nucleic acids in database searches.

[0064] (ii) fluorophore: The fluorophore in the BRET assay is a fluorescent protein.

[0065] Green fluorescent protein ("GFP") is a 238 amino acid residues polypeptide with amino acid residues 65 to 67 involved in the formation of the chromophore, which does not require additional substrates or cofactors to fluoresce (see, e.g, Prasher at al, 1992, Gene 111:229-233; Yang at a/, 1996, Nature Biotechnol. 14:1252-1256; and Cody et al, 1993, Biochemistry 32:1212-1218). Thus, in one embodiment, such a fluorophore is a green fluorescent protein (GFP) (referring to native Aequorea green fluorescent protein), and variants thereof.

[0066] A broad range of fluorescent protein genetic variants have been developed over the past several years that feature fluorescence emission spectral profiles spanning almost the entire visible light spectrum. Such variants of the GFP gene have been found useful to enhance expression and to modify excitation and fluorescence. Extensive mutagenesis efforts in the original jellyfish protein have resulted in fluorescent probes that range in color from blue to yellow. For example, substitution of a serine at position 65 to either alanine, glycine, isoleucine, or threonine results in mutant GFPs with a shift in excitation maxima and greater fluorescence than wild type protein when excited at 488 nm (see, e.g, Heim et al, 1995, Nature 373:663-664; U.S. Pat. No. 5,625,048; Delagrave et al, 1995, Biotechnology 13:151-154; Cormacketal, 1996, Gene 173:33-38; and Cramer at al, 1996, Nature Biotechnol. 14:315-319). Longer wavelength fluorescent proteins, emitting in the orange and red spectral regions, have been developed from the marine anemone Discosoma striata and reef corals belonging to the class Anthozoa. Still other species produce similar proteins having cyan, green, yellow, orange, red, and far-red fluorescence emission.
Thus, in another embodiment, GFPs are isolated from organisms other than the jellyfish, such as, but not limited to, the sea pansy, Renilla reriformis, or are variants thereof.

[0067] Thus, a fluorophore, as used herein, includes wild type green fluorescent protein and its variants, as well as fluorescent proteins and variants from other species.
Such

22 fluorophores are many, and are known to those of skill in the art. They include, but are not limited to:

= Green Fluorescent Proteins include GFP (wt), EGFP, Emerald, Superfolder GFP, Azami Green, mWasabi, TagGFP, TurboGFP, AcGFP, ZsGreen, T-Sapphire.

= Blue Fluorescent Proteins include Blue Fluorescent Protein (BFP), EBFP, EBFP2, Azurite, GFP2, GFP10, and mTagBFP;

= Cyan Fluorescent Proteins include Cyan Fluorescent Protein (CFP), ECFP, mECFP, Cerulean, CyPet, AmCyanl, Midori-Ishi Cyan, TagCFP, mCFPmm, and mTFP1 (Teal);

= Yellow Fluorescent Proteins include Yellow Fluorescent Protein (CFP), EYFP, Topaz, Venus, mCitrine, YPet, TagYFP, PhiYFP, ZsYellowl, and mBanana;

= Orange Fluorescent Proteins include Orange Fluorescent Protein (OFP), Kusabira Orange, Kusabira Orange2, mOrange, mOrange2, dTomato, dTomato-Tandem, TagRFP, TagRFP-T, DsRed, DsRed2, DsRed-Express (T1), DsRed-Monomer, and mTangerine; and = Red Fluorescent Proteins include Red Fluorescent Protein (RFP), mRuby, mApple, mStrawberry, AsRed2, mRFP1, JRed, mCherry, HcRedl, mRaspberry, dKeima-Tandem, HcRed-Tandem, mPlum, tdTomato, and AQ143.

[0068] Both green and yellow fluorescent proteins have been genetically engineered to create circular permutations of the original sequences that enable fusions to amino acids far removed from the normal amino and carboxy termini (abbreviated cpGFP and cpYFP).

[0069] The choice of a suitable fluorophore for use in a BRET assay will be known to one of skill in the art. In one embodiment, fluorophores include green fluorescent protein - wild type (GFP-wt), yellow fluorescent protein (YFP), Venus, Topaz, ZsYellow1, mOrange2, mKeima, blue fluorescent protein (BFP), cyan fluorescent protein (CFP), Tsapphire, mAmetrine, green fluorescent protein-2 (GFP2) and green fluorescent protein-10 (GFP10), or variants thereof.
Fluorescent proteins having an excitation peak close to 400 nm may be particularly suitable.

23 More particular examples of fluorophores include mAmetrine, cyan fluorescent protein (CFP), and GFP10.

[0070] Fluorescence Resonance Energy Transfer (FRET) Assay. Similar to BRET, FRET
involves the transfer of energy from an excited donor fluorophore to an adjacent acceptor fluorophore. For example, CFP and YFP, two color variants of GFP, can be used as donor and acceptor, respectively.

[0071] (i) fluorophore: The fluorophores in the FRET assay are fluorescent proteins, having the same properties as the fluorophore as defined above for the BRET assay.

[0072] Two fluorophores are employed in FRET, one as donor and one as acceptor. The term "donor fluorophore-acceptor pair," as used herein, means a donor fluorophore and an acceptor that has an absorbance spectrum overlapping the emission spectrum of the donor fluorophore. Where the first member of the pair is a donor fluorophore, the second member of the pair will be an acceptor. Where the first member of the pair is an acceptor, the second member of the pair will be a donor fluorophore.

[0073] Any of a number of fluorophore combinations can be selected for use in the FRET
embodiment described herein (see for example, Pesce et al., eds, Fluorescence Spectroscopy, Marcel Dekker, New York, 1971; White et al., Fluorescence Analysis: A
practical Approach, Marcel Dekker, New York, 1970; Handbook of Fluorescent Probes and Research Chemicals, 6th Ed, Molecular Probes, Inc., Eugene, Oreg., 1996; which are incorporated herein by reference). In general, a preferred donor fluorophore is selected that has a substantial spectrum of the acceptor fluorophore. Furthermore, it may also be desirable in certain applications that the donor have an excitation maximum near a laser frequency such as Helium-Cadmium 442 nM, Argon 488 nM, Nd:YAG 532 nm, He-Ne nm, etc. In such applications the use of intense laser light can serve as an effective means to excite the donor fluorophore. In certain preferred embodiments, the acceptor fluorophore has a substantial overlap of its excitation spectrum with the emission spectrum of the donor fluorophore. In some cases, the wavelength maximum of the emission spectrum of the acceptor moiety is preferably at least 10 nm greater than the wavelength maximum of the excitation spectrum of the donor moiety. Additional examples of useful FRET
labels include, e.g., those described in U.S. Pat. Nos. 5,654,419, 5,688,648, 5,853,992, 5,863,727,

24 5,945,526, 6,008,373, 6,150,107, 6,177,249, 6,335,440, 6,348,596, 6,479,303, 6,545,164, 6,849,745, 6,696,255, and 6,908,769 and Published U.S. Patent Application Nos.
2002/0168641, 2003/0143594, and 2004/0076979, the disclosures of which are incorporated herein by reference.

[0074] As indicated above, the donor and acceptor fluorophores should be capable of forming a FRET pair. Many suitable fluorophore pairs are familiar to those of skilled in the art.
In one embodiment, the FRET pair comprises one of CFP-YFP, GFP-mRFP1, YFP-mRFP1, GFP-RFP, sCFP3A-sYFP2, as well as sCFP3A in combination with circular permutations of sYFP2 (such as cp145, cp173, and cp229).

[0075] Circular permutations can be made by PCR. Such permutations have been described (see US 6,699,687 and Takeharu Nagai, Shuichi Yamada, Takashi Tominaga, Michinori Ichikawa and Atsushi Miyawaki (2004) PNAS 101(29): 10554-10559, incorporated by reference herein). They create variants of a FRET sensor with different orientations of the donor vs acceptor's chromophore.

LINKERS
[0076] The chromophores are each attached to the beta-arrestin molecule through independent linkers. Linkers may be employed to provide the desired conformation of the BRET/FRET label chromophores within the labeled compound, e.g., including the separation between chromophores in a BRET/FRET pair. The linkers may be bound to the C-terminal, the N-terminal, or at an intermediate position.

[0077] In one embodiment, the linkers are peptide linkers, typically ranging from 2 to 30 amino acids in length. The composition and length of each of the linkers may be chosen depending on various properties desired such as flexibility and aqueous solubility. For instance, the peptide linker may comprise relatively small amino acid residues, including, but not limited to, glycine; small amino acid residues may reduce the steric bulk and increase the flexibility of the peptide linker. The peptide linker may also comprise polar amino acids, including, but not limited to, serine. Polar amino acid residues may increase the aqueous solubility of the peptide linker. Furthermore, programs such as Globplot 2.3 (http://globplot.embl.de/cgiDict.py), may be used to help determine the degree of disorder and globularity, thus also their degree of flexibility.

[0078] By way of example, contemplated linkers include GDLRRALENSHASAGYQACGTGS and CLEDPRVPVAT. Short and relatively flexible linkers include GSAGT and KLPAT. Longer 15-residues long linkers, such as GSAGTGSAGTGSAGT (=3xGSAGT linker) and KLPATKLPATKLPAT (=3xKLPAT linker) are also contemplated; these latter 2 linkers are predicted (Globplot 2.3:
http://globplot.embl.de/cgiDict.py) to be disordered and non-globular sequences, and thus flexible. Alpha-helix structured rigid linker, REAAAREAAAREAAAR (16-residues long), is also contemplated.

[0079] The linkers may be attached to the beta-arrestin at the N-terminus, the C-terminus, or between the two termini of the beta-arrestin. When attaching in between the two termini, the linker may be attached, for instance to the first or second loop of the beta-arrestin.

METHODS
[0080] Expression vectors. Plasmids encoding Flag-AT1 aR, CCR5 (Pleskoff et al, 1997) and Myc-PAFR (Marrache et a!, 2002) were provided by S. Meloche, N. Heveker and S.
Chemtob, respectively (Universit6 de Montreal, Quebec, Canada) and WT R-arr2 was a generous gift from S. Marullo (Institut Cochin, Paris). Myc-V2R and HA-V1aR
(Terrillon eta!, 2003), Myc-02-AR (Hebert et al, 1996), Myc-b-OR (Petaja-Repo et a!, 2002), V2R-GFP
(Charest & Bouvier, 2003), 02-AR-GFP (Mercier et al, 2002).

[0081] BRET1 biosensors: 0-arr2-YFP (Angers et al, 2000) and Luc- 0-arr2 (Perroy et a!, 2003) have been described previously. Luc- 13-arr-YFP was generated by subcloning the coding sequence of enhanced YFP in-frame at the C terminus of (3-arr2 in pcDNA3.1-Luc-R-arr2, yielding Luc- R-arr-YFP with flexible spacers of 23 as between Luc and R-arr, and 10 as between R-arr and YFP. Mutation of arginine 169 into glutamate in Luc-R-arr (R169E)-YFP was generated by PCR site-directed mutagenesis using Luc-(3-arr-YFP. It should be noted that while the construct described here is specific for Luc- 3-arr-YFP, a construct leading to the production of a YFP-R-arr-Luc biosensor is feasible. Moreover, the resulting biosensor, YFP-R-arr-Luc, would be expected to function in the same manner as Luc-(3-arr-YFP. Similarly, DNA constructs may be devised for the specific expression of Luc-3-arr-GFP, GFP-[i-arr-Luc biosensors, and variants thereof.

[0082] BRET2 biosensors: Acceptor-beta-arrl/2-Rlucll, with Acceptor being either mAmetrine, sCFP3A or GFP10, were derived from previously published GFP10-EPAC-RlucIl fusion protein (Leduc at al. JPET 2009) by excising the EPAC coding sequence with Acc651-Hindill restriction enzymes and replacing it with a PCR-amplified coding sequence of human beta-arrestinl or beta-arrestin2. Sequence integrity was confirmed by DNA
sequencing.

[0083] Cell culture. Human embryonic kidney 293 (HEK293) cells and simian kidney fibroblast (COS) cells were maintained as described previously (Charest &
Bouvier, 2003).
Cells were transfected with the indicated plasmids using the calcium phosphate precipitation method (Sambrook et al, 1989) or the FuGENE 6 transfection reagent (Roche Applied Science, Laval, Canada) according to the manufacturer's protocol. The experiments were performed 48 h after transfection.

[0084] Fluorescence microscopy. To detect Myc-02-AR and Myc-V2R, cells were incubated with anti-Myc 9E10 monoclonal antibody (ascite fluid from our core facility) for 1 h at 4 C and then treated with the appropriate agonist (Sigma, Oakville, Canada) for 2 or 30 min at 37 C. Cells were then fixed and permeabilized before adding Texas-red-conjugated secondary antibody (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA). The samples were analysed by confocal laser-scanning microscopy using a Leica TCS SP1.
Measurements were as follows: YFP (green), Aex= 488 nm, Aem= 540/25nm; Texas red (red), Aex= 568 nm, Aem= 610/30nm.

[0085] BRET assays. Assessment of R-arr recruitment in BRET was performed as described previously (Charest & Bouvier, 2003). Briefly, cells were distributed in 96-well microplates (Corning, Corning, USA) and incubated with or without agonist for the indicated time at 25 C. The appropriate Luc substrate was added to a final concentration of 5 mM, either simultaneously with the agonist (time course) or following agonist treatment (single measurement or dose dependency), and readings were collected using a Multilabel Reader Mithras LB 940 (Berthold Technologies, Bad Wildbad, Germany). To detect BRET1 between Luc and YFP, coelenterazine h (Molecular Probes, Burlington, Canada) was used as substrate and light emission was detected at approximately 460-500nm (Luc) and approximately 510-550nm (YFP), whereas for BRET2 detection (Luc and GFP), coelenterazine 400a (Perkin-Elmer, Wellesley, MA, USA or Biotium Inc, Hayward, CA, USA) and filters at approximately 330-470nm (Luc) and approximately 495-535nm (GFP2/GFP10) were used. (Broadly speaking, ranges for the detection of light emission for BRET1 are approximately 440-510nm (Luc) and 510-570nm (YFP), while those for BRET2 are approximately 320-490nm (Luc) and 490-550nm (GFP)). Those for BRET3 are ????.
The BRET signal was determined by calculating the ratio of the light emitted by the fluorescent acceptor and the light emitted by Luc. The values were corrected by subtracting the background BRET signals detected when Luc-R-arr was expressed alone.
Expression levels of the different receptors transfected were verified by enzyme-linked immunosorbent assay (ELISA) (Charest & Bouvier, 2003).

[0086] Receptor endocytosis assay. Receptor endocytosis was measured by ELISA
as described previously (Charest & Bouvier, 2003).

100871 Z'-factor determination. HEK293T cells were cultured in DMEM
supplemented with 10% fetal bovine serum, 100 units/ml penicillin and streptomycin (Wisent Inc).
3.0x106 cells were seeded in 10cm dishes. Transient transfection was performed using polyethylenelmine (PEI; Polysciences) at a DNA:PEI ratio. 24h post-transfection, cells were detached, seeded in pretreated poly-L-ornithine hydrobromide (Sigma-Aldrich) 96-well white plates at 50,000 cells per well, and re-incubated at 37 C for an additional 24h before being processed. Cells were washed once with Tyrode's buffer directly in the 96-well plates and incubated in buffer with or without 100nM of AVP for 25 to 35 min. Coelenterazine 400A was added to a final concentration of 5pM in Tyrode's buffer 5min before reading. Readings were collected as a sequential integration of the signals detected in the 480 i 20 and 530 20nm window for the Rlucll Renilla luciferase and GFP10 light emissions, respectively. The BRET
signal was determined by calculating the ratio of the light intensity emitted by the GFP10 over the light intensity emitted by the RLucll.

RESULTS
[0088] Double-brilliance Q-arr sensor (BRET1): Inspired by previous reports of intramolecular fluorescence resonance energy transfer (FRET)-based biosensors (Zhang et al, 2002) showing that resonance energy transfer (RET) is sensitive to changes in the relative positions of the donor and acceptor molecules, the feasibility of monitoring whether conformational changes of R-arr using an intramolecular BRET approach was assessed. A
double-brilliance R-arr was engineered in which Luc was fused to the N
terminus of R-arr2 and YFP to its C terminus, yielding Luc- P-arr-YFP (Fig 1). To test the functionality of Luc-P-arr-YFP, the ability of this molecule to be recruited to agonist-stimulated class A (receptors interacting transiently with Parr) 02-adrenergic receptor (P2-AR) and class B
(receptors interacting stably with Parr) V2 vasopressin receptor (V2R) by fluorescence microscopy was determined. As shown in Fig 2A, agonist stimulation led to rapid translocation of Luc- -arr-YFP to the plasma membrane, colocalizing with Myc-tagged 02-AR and V2R (Myc-P2-AR;
Myo-V2R). The patterns of Luc- P-arr-YFP interaction were consistent with those observed for class A (transient P-arr interaction) and B (stable P-arr association) receptors in similar experiments using a P-arr-green fluorescent protein (GFP) conjugate (Oakley at a/, 2000).
Indeed, whereas Luc-f3-arr-YFP was recruited to both P2-AR and V2R after 2 min of stimulation, it returned to the cytoplasm after 30 min in Myc-P2-AR-expressing cells but remained colocalized with Myc-V2R in endocytic vesicles.

[0089] To quantitatively assess the recruitment of Luc-P-arr-YFP to agonist-activated GPCRs, an intermolecular BRET2 assay that takes advantage of the different spectral properties of Luc substrates that allow energy transfer to different fluorescent acceptors (Milligan, 2004) was used. Luc-P-arr-YFP was transiently coexpressed with the receptors, and the agonist-induced BRET2 between Luc- P-arr-YFP and either 02-AR-GFP or GFP was measured in the presence of DeepBlueCTM coelenterazine, allowing transfer of energy to GFP. As shown in Fig 2, agonist stimulation promoted a time-dependent (Fig 2B) and dose-dependent (Fig 2C) increase in BRET2, reflecting the recruitment of Luc-13-arr-YFP to the receptors. Similar kinetics and EC50 were obtained for the recruitment of both Luce-arr-YFP and Luc-f3-arr, indicating that double-brilliance P-arr is as efficiently recruited to the receptors as the singly conjugated construct. It should be noted that, although the maximum agonist-promoted BRET increase observed with the class A 02-AR is less than that observed with the class B V2R, the stability of the signals was similar, indicating that the signal observed with 02-AR reflects a steady state corresponding to constant association and dissociation of P-arr from the activated receptors.

[0090] To assess the biological activity of Luc- P-arr-YFP, its capacity to promote receptor endocytosis in COS cells, which express low endogenous levels of P-arr, was tested. As shown in Fig 2D, agonist-promoted P2-AR and V2R endocytosis was considerably increased when overexpressing Luc-P-arr-YFP. Even though this increase in receptor endocytosis was not as pronounced as that obtained by the overexpression of wild-type (WT) i3-arr, it suggests that Luc-R-arr-YFP retains significant biological activity.

[0091] Agonist-induced conformational changes of R-arr: To assess whether Luc-R-arr-YFP could be used to monitor the conformational rearrangement of parr upon receptor activation, the construct was expressed with and without V2R, and BRET was measured in the presence of coelenterazine h, allowing transfer of energy to YFP. As shown In Fig 3A, an important basal BRET signal could be measured in cells transfected with Luc-(3-arr-YFP, reflecting the proximity of the energy donor and acceptor in the construct.
Arginine vasopressin (AVP) stimulation of cells coexpressing V2R led to a significant increase in BRET, suggesting movement of Luc and YFP relative to each other. To rule out the possibility that this increased signal results from intermolecular BRET
between individual Luc-R-arr-YFP molecules brought together through oligomerization (Hirsch et al, 1999) or clustering at the plasma membrane, the occurrence of BRET in cells transiently expressing Luc-p-arr and (3-arr-YFP was determined. In transfection conditions leading to equivalent fluorescence and luminescence levels as those obtained in Luc-P-arr-YFP-expressing cells, coexpression of Luc-f3-arr and R-arr-YFP led to the detection of only a marginal basal BRET
that could not be modulated by V2R stimulation (Fig 3A). This observation demonstrates that the AVP-induced increase in BRET signal observed in cells transfected with Luc-(3-arr-YFP
results from a change in intramolecular BRET. As variations in RET can reflect changes in both the distance and orientation between the energy donor and acceptor molecules (Andrews & Demidov, 1999), the observed agonist-promoted increase in the Luc -f3-arr-YFP
intramolecular BRET could indicate that the N terminus and C terminus are either brought closer or are in a more permissive BRET orientation following activation.

[0092] To further characterize the agonist-induced change in the conformation of (3-arr, the kinetics and dose dependency of AVP-mediated BRET increase were assessed. Real-time BRET measurements show a time-dependent AVP-induced conformational change of R-arr, with half-time of maximal BRET increase (t1/2) of 5.1 1.5 min (Fig 3B). The kinetics are significantly slower (P < 0.02) than that of the AVP-induced recruitment of 13-arr (tl/2=0.8 0.2 min; Fig 2B, right panel), suggesting that the conformational change observed in Luc-13-arr-YFP occurs after its initial recruitment to the activated V2R. The difference in kinetics cannot result from inter-experimental variations because similar results were obtained when the two events were measured in the same cell population expressing V2R-GFP
and Luc-[3-arr-YFP (data not shown). Despite the difference in kinetics, the efficacy of AVP to induce a conformational change in Luc-P-arr-YFP (Fig 3C) was similar to that observed for R-arr recruitment (Fig 2C, right panel), indicating that these two events are directly linked and reflect the binding affinity of V2R for AVP (KD -1 x 10-9 M).

[0093] The observed kinetic lag between P-arr recruitment and its conformational change could be consistent with the proposal that inactive P-arr is first recruited to the activated GPCR where its interaction with the GRK-phosphorylated residues subsequently induces the release of its C-tail (Gurevich & Gurevich, 2003). Alternatively, such a lag could indicate that the intramolecular BRET changes observed with Luc-[i-arr-YFP result from the subsequent recruitment of P-arr-interacting proteins (e.g. clathrin and AP2 or signalling proteins such as c-Src, Raft, ERK1/2, ASK1 and JNK3) to the receptor-bound P-arr (Lefkowitz &
Whalen, 2004). Interestingly, a P-arr (R169E) mutant shown to bind to GPCRs in a phosphorylation-independent manner, probably as a result of a constitutively open conformation (Kovoor et al, 1999) resulted, when inserted between Luc and YFP (Luc- P-arr(R169E)-YFP), in basal and AVP-stimulated BRET signals similar to those observed with WT Luc-P-arr-YFP
(Fig 4). This indicates that the engagement of Parr by the activated receptor can be detected by the double brilliance Parr independently of the phosphorylation state of the receptor.

[0094] A general biosensor to monitor GPCR activity: To assess whether Luc-P-arr-YFP
could be used as a general GPCR activity sensor, a determination of whether its agonist-induced conformational change could be promoted by other receptors was made, particularly those of class A, which are believed to interact only transiently with P-arr.
Recruitment of Luc-p-arr-YFP and agonist promoted intramolecular BRET were assessed in cells coexpressing different receptors of class A (p2-AR, V1 vasopressin receptor (V1 aR), d-opioid receptor (6-OR)) and class B (platelet-activating factor receptor (PAFR), CC
chemokine receptor type 5 (CCR5), angiotensin receptor type 1a (AT1aR)). As shown in Fig 5A, agonist stimulation efficiently induced the recruitment of Luc-p-arr YFP to the plasma membrane, with the expected interaction patterns for all class A (transient) and class B
(stable) receptors. In all cases, activation of Luc-P-arr-YFP mediated by class A and B
receptors was accompanied by a significant increase in BRET (Fig 5B). Interestingly, although the kinetics and stability of the BRET increase were found to be similar for receptors of class A
and B (data not shown), a tendency of class A receptors to induce smaller BRET
increases was observed. As previously noted when comparing the BRET-detected recruitment of P-arr to class A P2-AR and class B V2R (Fig 2B), this probably indicates that the BRET assays provide a steady-state signal reflecting continuous rounds of association-dissociation cycles.
In any case, these results suggest that Luc-P-arr-YFP can be used as a general biosensor to monitor GPCR activity and that the interaction can be monitored for extended periods of time making it compatible with its use in high through put screening assays that request long lived signals. When compared with the intermolecular BRET-based P-arr recruitment assays (Angers et al, 2000; Bertrand at al, 2002), double-brilliance P-arr avoids the difficulty of expressing the appropriate ratio of energy donor and acceptor constructs and allows the study of unmodified GPCRs.

[0095] The interaction of P-arr with the GRK-phosphorylated GPCRs is thought to induce the release of P-arr's C-tail and the opening of its structure (Gurevich and Gurevich 2003), subsequently leading to the recruitment of Parrestin-interacting proteins (Lefkowitz and Whalen 2004). To assess if the conformational change of P-arr detected with the double brilliance P-arr could also be detected using Parr mutants believed to be constitutively in the open state, assessment was made of the agonist-promoted BRET signal of two other P-arr mutants (P-arr(3A): 1387A, V388A, F389A; P-arr(IV): 1387A, V388A),inserted between Luc and YFP (Luc-Parr(3A)-YFP and Luc-Parr(IV)-YFP). These mutant P-arrs are believed to be constitutively active due to the disruption of the polar core keeping Parrestin In a closed and inactive conformation (Gurevich 1998). As shown in Fig 6, while the basal BRET
signal observed with each Luc-Parr-YFP constitutively active mutant (Luc-Parr(3A)-YFP
and Luc-Parr(IV)-YFP) was found to be similar to that of wild-type Luc-Parr-YFP, the agonist-induced BRET increase was significantly reduced by the mutations (Fig 6, inset).

[0096] In addition to agonists, the activity of ligands with inverse agonist efficacy towards specific signalling pathways can be detected by the double brilliance P-arr.
As shown in Fig 7, the V2R inverse agonist SR121463 that inhibits cyclic AMP production can promote an increase in the BRET signal in cells co-expressing wild type V2R and Luc-(3-arr-YFP.

[0097] Double brilliance P-arr may also prove to be an effective tool in the study of the increasingly diverse roles played by P-arr, such as its involvement with receptors other than GPCRs and diverse signaling molecules in different systems (Fig. 8). A list of some of the proteins that have been shown to interact with Parr and which activity could be monitored by double brilliance P-arr is presented in Table 1. The spectrum of receptors capable of utilizing P-arr for endocytosis via clathrin binding sites has significantly increased (Lefkowitz and Whalen 2004a). For exemple, P-arr appears to be required for engulfing Frizzled-4, an atypical seven-transmembrane domain receptor, through interaction with the adaptor protein Dishevelled-2 phosphorylated by PKC (Chen et al. 2003a); for the endocytosis of receptors with serine/threonine kinase activity such as the transforming growth factor 0 receptor (TGF-PR), in a manner dependent on the phosphorylation of Rill by RII (Chen et al.
2003b); as well as for the endocytosis of the IGF1 receptor, in a manner that is independent from its phosphorylation (Dalle et al. 2001). This indicates that the Parr double brillance could be a general biosensor of the activity of many distinct receptors and signalling molecules.

Table 1: List of proteins capable of interacting with P-arrestin Binding Protein -arrestin isoform jyjLe of protein Clathrin -arr 1, 2 trafficking AP2 -arr 1, 2 trafficking NSF -arr I trafficking ARF6 -arr 2 1 Small G/GEFs ARNO -arr 2 Small G/GEFs Rai-GDS -arr 1, 2 Small G/GEFs RhoA -arr 1 Small G/GEFs MAPK cascade components: Signaling ASK1 P-arr 1, 2 c-Raf-1 P-arr 1, 2 JNK3 P-arr 2, 1 ERK2 -arr 1, 2 Nonreceptor tyrosine kinases: signaling c-Src P-arr 1, 2 Yes P-arr I
Hck P-arr 1 Fgr -arr 1 Others: signaling Mdm2 P-arr 1, 2 IKBa P-arr 1, 2 PDE4D family P-arr 1, 2 Dishevelled P-arr 1, 2 PP2A -arr I
(Lefkowitz & Shenoy, 2005) [0098] Double-brilliance 3-arr sensor (BRET2): In addition to the constructs described above, the following BRET2-based beta-arrestin 1 and 2 sensors: Acceptor (mAmetrine;

sCFP3A; GFP10)-GSAGT-RArrestinl/2-KLPAT-Rlucll were also made (Fig 9a), using similar techniques, namely:

Ametrine-h(3arl r-Rlucll, Ametrine-hparr2-Rlucl I, UP-hparrl-Rlucll, CFP-h3arr2-RluclI, GFP10-h3arr1-Rlucll, and GFP10-hF3arr2-Rlucll.

[0099] An enhanced Rluc variant (RIuc Ii) was used with the BRET2 versions as it provides a sustained and a stronger signal with coelenterazine 400a (200-400 times) than with the WT
Rluc. The orientation of the BRET tags and nature of the linkers in these constructs differ from the BRET1 version. In contrast to the BRET1 sensors, these structure leads to a decrease BRET signal in response to an agonist-promoted GPCR activation. As shown in figure 9B and 2B, this inversion of the BRET signal between the BRET1 and BRET2 versions of the beta-arrestin db sensors still lead to similar kinetics and dose-responses to an AVP
stimulation of V2R. Both beta-arrestin1 and 2 sensors are functional and give similar responses for the same receptor activation (Fig 9B).

[00100] The inversion of the signal between BRET1 and BRET2 provides a user with complementary tools that could be useful for high throughput. For example, having sensors with different responses (such as BRET1 and BRET2) could be useful to identify compounds that were mislabeled as active (ie. false positives) due to their colour and not because of their activity or binding.

[00101] In certain embodiments when a change in the orientation of the BRET
tags (fluorophores and chromophores), leads to a difference in BRET response, advantages may be realized. Orientation of the tags and possibly the linkers as well, can be adjusted and result in a change in the nature of the BRET signal (increase or decrease).
Such modulation of the BRET response through the orientation of the tags could provide the user with a complementary tool that can be used in screening assays, especially In high throughput screening assays, for example.

[00102] Both orientations of the BRET tags lead to sensors with similar Z' factors (reflecting the robustness of the assay) that could be adapted to a high throughput screening assay (HTS). The observed responses need not be attributed to the nature of the BRET
tags. The nature of the tags may not necessarily cause the different BRET
responses observed with the BRET1 vs BRET2 sensors.

[00103] The orientation of the donor vs acceptor may be a possible cause of the inversion of causing this inversion of the sensor's response. A BRET2 construct made with the same orientation of the BRET1 construct shows a consistent increase in BRET upon stimulation.

[00104] The selection of a linker may impact the amplitude of the sensor's response.
[00105] Having sensors with responses going in different directions could be useful in the validation process of an HTS screen as they could be used to identify compounds mislabelled as active because of their color and not because of their activity.

[00106] The Z'-factor Is a reflection of the robustness of an assay and should vary depending on the experimental conditions and receptor used. With cells transiently expressing both V2R and sensors a Z'-factor between 0.43 and 0.63 (fig 10) was obtained for both BRET1 and BRET2 versions of the beta-arrestin db sensors, providing a robust assay for monitoring GPCR activation with both full and partial agonists (fig 9C). Using cell lines stably expressing both receptor and sensor, an even better Z'-factor is expected and thus be sufficient to develop a high throughput screening (HTS) assay. Even though the response of the BRET1 vs BRET2 sensors is going in different directions, similar Z' factors are obtained.

[00107]
[00108] Since the fluorescent energy transfer is based on stimulatory principles such as BRET, a biosensor as described herein based on FRET instead of BRET would also be expected to function and is included herein.

[00109] In summary, the above is believed to be the first real-time monitoring of agonist-promoted conformational changes of 13-arr in living cells using a double-brilliance S-arr intramolecular BRET-based biosensor. The conformational rearrangement of the [i-arr molecule and its interaction with other proteins reflects its transition from an inactive state to a biologically active state that follows its initial recruitment to activated GPCRs and involves the relative movement of the C-and N-terminus leading to a change in the BRET
signal Beta-arrestin db sensors offer a robust assay for GPCR activation and characteristics (unimolecular structure, ratiometric signal and recruited to most GPCRs) that could be amenable to large-scale screening campaigns. In conclusion, double brilliance R-arr represents the first intramolecular BRET-based biosensor that allows the monitoring of protein conformational changes. This should lead the way to the development of similar tools to study other proteins believed to undergo significant conformational rearrangement linked to their function.

[00110] Although the present invention has been described by way of specific embodiments and examples thereof, with a particular focus on G protein-coupled receptors, it should be noted that it will be apparent to persons skilled in the art that modifications may be applied to the present particular embodiments described.

LIST OF REFERENCES:

1. Andrews DL, Demidov AA (1999) Resonance Energy Transfer. Chichester, UK:
Wiley 2. Angers S, Salahpour A, Joly E, Hilairet S, Chelsky D, Dennis M, Bouvier M
(2000) Detection of R2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET). Proc Natl Acad Sci USA 97: 3684-3689 3. Azzi M, Charest PG, Angers S, Rousseau G, Kohout T, Bouvier M, Pineyro G
(2003) Arrestin-mediated activation of MAPK by inverse agonists reveals distinct active conformations for G protein-coupled receptors. PNAS 100: 11406-11411 4. Bertrand L, Parent S, Caron M, Legault M, Joly E, Angers S, Bouvier M, Brown M, Houle B, Menard L (2002) The BRET2/arrestin assay in stable recombinant cells: a platform to screen for compounds that interact with G protein-coupled receptors (GPCRs). J
Receptor Signal Transduction Res 22: 533-541 5. Charest PG, Bouvier M (2003) Palmitoylation of the V2 vasopressin receptor carboxyl tail enhances Q-arrestin recruitment leading to efficient receptor endocytosis and activation. J Biol Chem 278: 41541-41551 6. Chen W, Kirkbride KC, How T, Nelson CD, Mo J, Frederick JP, Wang XF, Lefkowitz RJ, Blobe GC (2003) Beta-arrestin 2 mediates endocytosis to type III TFG-beta receptor and down-regulation of its signalling. Science 301 (5638): 1394-1397 7. Dalle S, Ricketts W, Imamura T, Voilenweider P, Olefsky JM (2001) Insulin and insulin-like growth factor I receptors utilize different G protein signalling components. J Biol Chem 276 (19): 15688-15695 8. Gurevich W, Benovic JL (1993) Visual arrestin interaction with rhodopsin.
Sequential multisite binding ensures strict selectivity toward lightactivated phosphorylated rhodopsin.
J Biol Chem 268: 11628-11638 9. Gurevich W, Gurevich EV (2003) The new face of active receptor bound arrestin attracts new partners. Structure (Camb) 11: 1037-1042 10. Han M, Gurevich W, Vishnivetskiy SA, Sigler PB, Schubert C (2001) Crystal structure of R-arrestin at 1.9 A : possible mechanism of receptor binding and membrane translocation. Structure (Camb) 9: 869-880 11. Hebert TE, Moffett S, Morello JP, Loisel TP, Bichet DG, Barret C, Bouvier M (1996) A
peptide derived from a R2-adrenergic receptor transmembrane domain inhibits both receptor dimerization and activation. J Biol Chem 271: 16384-16392 12. Hirsch JA, Schubert C, Gurevich W, Sigler PB (1999) The 2.8A crystal structure of visual arrestin: a model for arrestin's regulation. Cell 97: 257-269 13. Kovoor A, Celver J, Abdryashitov RI, Chavkin C, Gurevich W (1999) Targeted construction of phosphorylation-independent R-arrestin mutants with constitutive activity in cells. J Biol Chem 274: 6831-6834 14. Leduc M, Breton B, Gales C, Le Gouill C, Bouvier M, Chemtob S, Heveker N
(2009) Functional selectivity of natural and synthetic prostaglandin EP4 receptor ligands J
Pharmacol Exp Ther. 331(1):297-307 15. Lefkowitz RJ, Whalen EJ (2004) (3-anrestins: traffic cops of cell signalling. Curr Opin Cell Biol 16: 162-168 16. Lefkowitz RJ, Shenoy SK (2005) Transduction of Receptor Signals by (3-arrestins.
Science 308: 512-517 17. Loening AM, Fenn TD, Wu AM, Gambhir SS. (2006) Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output. Protein Eng Des Sel.
19(9):391-400.

18. Lin FT, Miller WE, Luttrell LM, Lefkowitz RJ (1999) Feedback regulation of R-arrestinl function by extracellular signal-regulated kinases. J Biol Chem 274: 15971-19. Lin FT, Chen W, Shenoy S, Cong M, Exum ST, Lefkowitz RJ (2002) Phosphorylation of 0-arrestin2 regulates its function in internalization of b(2)-adrenergic receptors.
Biochemistry 41: 10692-10699 20. Luttrell LM, Lefkowitz RJ (2002) The role of R-arrestins in the termination and transduction of G-protein-coupled receptor signals. J Cell Sci 115: 455-465 21. Marrache AM et a/ (2002) Proinflammatory gene induction by plateletactivating factor mediated via its cognate nuclear receptor. J Immunol 169: 6474-6481 22. Mercier JF, Salahpour A, Angers S, Breit A, Bouvier M (2002) Quantitative assessment of b1- and 32-adrenergic receptor homo- and heterodimerization by bioluminescence resonance energy transfer. J Biol Chem 277: 44925-44931 23. Milligan G (2004) Applications of bioluminescence- and fluorescence resonance energy transfer to drug discovery at G protein-coupled receptors. Eur J Pharm Sci 21:

24. Oakley RH, Laporte SA, Holt JA, Caron MG, Barak LS (2000) Differential affinities of visual arrestin, R-arrestin1, and R-arrestin2 for G proteincoupled receptors delineate two major classes of receptors. J Biol Chem 275: 17201-17210

25. Oakley RH, Laporte SA, Holt JA, Barak LS, Caron MG (2001) Molecular determinants underlying the formation of stable intracellular G proteincoupled receptor-p-arrestin complexes after receptor endocytosis. J Biol Chem 276: 19452-19460

26. Perroy J, Adam L, Qanbar R, Chenier S, Bouvier M (2003) Phosphorylationindependent desensitization of GABA(B) receptor by GRK4. EMBO J 22: 3816-3824

27. Petaja-Repo UE, Hogue M, Bhalla S, Laperriere A, Morello JP, Bouvier M
(2002) Ligands act as pharmacological chaperones and increase the efficiency of d-opioid receptor maturation. EMBO J 21: 1628-1637

28. Pleskoff 0, Treboute C, Brelot A, Heveker N, Seman M, Alizon M (1997) Identification of a chemokine receptor encoded by human cytomegalovirus as a cofactor for HIV-1 entry.
Science 276: 1874-1878

29. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press

30. Terrillon S, Durroux T, Mouillac B, Breit A, Ayoub MA, Taulan M, Jockers R, Barberis C, Bouvier M (2003) Oxytocin and vasopressin V1 a and V2 receptors form constitutive homo- and heterodimers during biosynthesis. Mol Endocrinol 17: 677-691

31. Vishnivetskiy SA, Hirsch JA, Velez MG, Gurevich YV, Gurevich W (2002) Transition of arrestin into the active receptor-binding state requires an extended interdomain hinge. J
Biol Chem 277: 43961-43967

32. Xiao K, Shenoy SK, Nobles K, Lefkowitz RJ (2004) Activation dependent conformational changes in R-arrestin 2. J Biol Chem 279: 55744-55753.

33. Zhang J, Campbell RE, Ting AY, Tsien RY (2002) Creating new fluorescent probes for cell biology. Nat Rev Mol Cell Biol 3: 906-918Zhang JH, Chung TD, Oldenburg KR.
(1999) A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomol Screen. 4(2):67-73.

Claims (41)

WHAT IS CLAIMED IS:

1. A resonance energy transfer (RET) biosensor comprising an arrestin tagged with a first and a second chromophore, wherein said first chromophore is a fluorophore and said second chromophore is a fluorophore or a bioluminophore.

2. The biosensor according to claim 1, wherein said arrestin is .beta.-arrestin-1 (arrestin-2).

3. The biosensor according to claim 1, wherein said arrestin is .beta.-arrestin-2 (arrestin-3).

4. The biosensor according to claim 1, wherein said arrestin is arrestin-1 or arrestin-4.

5. The biosensor according to claim 1, wherein said second chromophore is a bioluminophore and the RET is bioluminescence resonance energy transfer (BRET).

6. The biosensor according to claim 5, wherein said bioluminophore is Renilla luciferase or a mutant form of Renilla luciferase.

7. The biosensor according to claim 6, wherein said bioluminophore is RIucII
(A55T/C124A/M185V).

8. A biosensor as defined in claim 1, wherein said fluorophore is a fluorescent protein.

9. The biosensor as defined in claim 8, wherein said fluorophore is green fluorescent protein or a variant thereof.

10. The biosensor of claim 9, wherein said fluorophore is YFP, mAmetrine, cyan fluorescent protein (CFP), or GFP10.

11. A biosensor as defined in claim 5, which is selected from the group consisting of:
Luc-.beta.-arr-YFP, YFP-.beta.arr-Luc, Luc-.beta.-arr(3A)-YFP, Luc-.beta.-arr(IV)-YFP, Luc-parr(R169E)-YFP, Ametrine-h.beta.ar1-RIucII, Ametrine-h.beta.arr2-RIucII, CFP-h.beta.arr1-RIucII, CFP-h.beta.arr2-RIucII, GFP10-h.beta.arr1-RIucII, and GFP10-h.beta.arr2-RIucII.

12. The biosensor as defined in claim 5, wherein the fluorophore is at the N-terminus of the arrestin and the bioluminophore is at the C-terminus of the arrestin.

13. The biosensor as defined in claim 12, wherein said fluorophore is green fluorescent protein or a variant thereof,

14. The biosensor of claim 13, wherein said fluorophore is YFP, mAmetrine, cyan fluorescent protein (CFP), or GFP10.

15. The biosensor according to any one of claims 12 to 14, wherein said bioluminophore is Renilla luciferase or a mutant form of Renilla luciferase.

16. The biosensor according to claim 15, wherein said mutant form of Renilla luciferase is RIucII (A55T/C124A/M 185V).

17. The biosensor as defined in claim 5, wherein the fluorophore is at the C-terminus of the arrestin and the bioluminophore is at the N-terminus of the arrestin.

18. The biosensor as defined in claim 17, wherein said fluorophore is green fluorescent protein or a variant thereof.

19. The biosensor of claim 18, wherein said fluorophore is YFP, mAmetrine, cyan fluorescent protein (CFP), or GFP10.

20. The biosensor according to any one of claims 17 to 19, wherein said bioluminophore is Renilla luciferase or a mutant form of Renilla luciferase.

21. The biosensor of claim 5, wherein one of the fluorophore or bioluminophore is linked to a position between the C and N-termini.

22. The biosensor as defined in claim 21, wherein the bioluminophore is linked to the C-terminus of the arrestin and the fluorophore is linked internally to the arrestin.

23. The biosensor of claim 22, wherein the fluorophore is YFP, mAmetrine, cyan fluorescent protein (CFP), or GFP10, and the bioluminophore is Renilla luciferase or a mutant form of Renilla luciferase.

24. The biosensor according to any one of claims 1 to 4, wherein both said first and said second chromophores are fluorophores and the RET is fluorescence resonance energy transfer (FRET).

25. The biosensor of claim 24, wherein the fluorophore donor is CFP or a variant thereof, and the fluorophore acceptor is a YFP or a variant thereof.

26. The biosensor of claim 25, wherein the YFP is a non-circularly permuted sYFP2 or a circularly permuted sYFP2.

27. A method of identifying candidate molecules that bind to a receptor comprising:

a) screening candidate molecules for activation of the biosensor according to any one of claims 17 to 20, the determine a candidate population; and b) screening said candidate population for activation of the biosensor according to any one of claims 12 to 16 to identify candidate molecules that bind to receptors.

28. The method of claim 27 wherein the receptor is a Frizzled protein receptor or a G
protein-coupled receptor (GPCR).

29. The method according to claim 27 or 28 wherein said receptor is chosen from the group consisting of Frizzled 4(Fz4), .beta.2AR, V1 vasopressin receptor (V1aR), V2 vasopressin repressor (V2R), delta opioid receptor, platelet-activating factor receptor, CC chemokine receptor type 5, and angiotensin receptor type 1a.

30. The method according to any one of claims 27 to 29 wherein the activation of the biosensor in a) is an increase in BRET signal.

31. The method according to any one of claims 27 to 30 wherein the activation of the biosensor in b) results in a decrease in BRET signal.

32. The method according to any one of claims 27 to 31 wherein the candidate molecule that binds the receptor is an agonist, inverse agonist, partial agonist, antagonist or allosteric regulator.

33. Use of a biosensor as defined in any one of claims 17 to 20 and a biosensor of any one of claims 12 to 16 for assaying receptor activity.

34. Use of a biosensor as defined in any one of claims 17 to 20 and a biosensor of any one of claims 12 to 16 for identifying agonists, inverse agonists, partial agonists, antagonists or allosteric regulators.

35. A kit for evaluating receptor binding comprising:
a biosensor of any one of claims 17 to 20; and a biosensor of any one of claims 12 to 16.

36. A kit for evaluating receptor binding to identify candidate molecules that bind to the receptor, said kit comprising:

a biosensor of any one of claims 17 to 20;

a biosensor of any one of claims 12 to 16; and instructions for use in the method according to any one of claims 27 to 32.

37. The method of claim 27 wherein screening candidate molecules comprises:
identifying an agonist or inverse agonist for the receptor by incubating (i) cells co-expressing the receptor and the biosensor with a potential agonist or inverse agonist, or (ii) an isolated receptor and the biosensor with a potential agonist or inverse agonist; adding a suitable substrate to detect bioluminescence resonance energy transfer (BRET) in said biosensor; detecting a BRET signal; and comparing the BRET
signal with a BRET signal obtained under similar conditions in the absence of the potential agonist or inverse agonist, wherein the potential agonist or inverse agonist is identified as an agonist or inverse agonist if a change in BRET signal level is observed.

38. The method as defined in claim 37, wherein the BRET signal is evaluated by detecting light emissions at about 440-510 nm and at about 510-570 nm.

39. The method as defined in claim 37, wherein the BRET signal is evaluated by detecting light emissions at about 320-490 nm and at about 490-550 nm.

40. The method as defined in claim 37, wherein said receptor is a Frizzled protein receptor or a G protein-coupled receptor.

41. The method as defined in claim 37, wherein said receptor is selected from the group consisting of Frizzled 4 (Fz4), .beta.2AR, V1 vasopressin receptor (V1aR), V2 vasopressin receptor (V2R), delta-opioid receptor (.delta.OR), platelet-activating factor receptor (PAFR), CC chemokine receptor type 5 (CCR5), and angiotensin receptor type 1a (AT1aR).