JP2017532043A5 - - Google Patents
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- Publication number
- JP2017532043A5 JP2017532043A5 JP2017519813A JP2017519813A JP2017532043A5 JP 2017532043 A5 JP2017532043 A5 JP 2017532043A5 JP 2017519813 A JP2017519813 A JP 2017519813A JP 2017519813 A JP2017519813 A JP 2017519813A JP 2017532043 A5 JP2017532043 A5 JP 2017532043A5
- Authority
- JP
- Japan
- Prior art keywords
- erf1
- combination
- mutant erf1
- mutant
- unnatural amino
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102100030667 Eukaryotic peptide chain release factor subunit 1 Human genes 0.000 claims 36
- 101710175705 Eukaryotic peptide chain release factor subunit 1 Proteins 0.000 claims 36
- 108020004705 Codon Proteins 0.000 claims 19
- 150000001413 amino acids Chemical class 0.000 claims 17
- 230000035772 mutation Effects 0.000 claims 16
- 210000004027 cell Anatomy 0.000 claims 14
- 108090000765 processed proteins & peptides Proteins 0.000 claims 14
- 150000007523 nucleic acids Chemical group 0.000 claims 13
- 229920001184 polypeptide Polymers 0.000 claims 12
- 102000004196 processed proteins & peptides Human genes 0.000 claims 12
- 238000010348 incorporation Methods 0.000 claims 11
- 238000000034 method Methods 0.000 claims 9
- 108020004707 nucleic acids Proteins 0.000 claims 8
- 102000039446 nucleic acids Human genes 0.000 claims 8
- 102000004169 proteins and genes Human genes 0.000 claims 7
- 108090000623 proteins and genes Proteins 0.000 claims 7
- 210000003527 eukaryotic cell Anatomy 0.000 claims 6
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 5
- 125000003275 alpha amino acid group Chemical group 0.000 claims 5
- 102220498965 Phosphatidylinositol 4-phosphate 5-kinase type-1 beta_Y125F_mutation Human genes 0.000 claims 4
- 102000052866 Amino Acyl-tRNA Synthetases Human genes 0.000 claims 2
- 108700028939 Amino Acyl-tRNA Synthetases Proteins 0.000 claims 2
- 102220473604 Cytochrome b5_M51A_mutation Human genes 0.000 claims 2
- 102220590746 Mediator of RNA polymerase II transcription subunit 13_T58K_mutation Human genes 0.000 claims 2
- 102220558658 Nuclear protein 1_E55A_mutation Human genes 0.000 claims 2
- 102220558654 Nuclear protein 1_E55Q_mutation Human genes 0.000 claims 2
- 102220643725 Prolactin-inducible protein_S60T_mutation Human genes 0.000 claims 2
- 102220530815 Transcription factor SOX-9_S64D_mutation Human genes 0.000 claims 2
- 102220247983 rs761483326 Human genes 0.000 claims 2
- YMBQDEOCKOLCJG-AXDSSHIGSA-N (2S)-2-amino-6-[(2-methylcycloprop-2-en-1-yl)methoxycarbonylamino]hexanoic acid Chemical compound CC1=CC1COC(=O)NCCCC[C@H](N)C(O)=O YMBQDEOCKOLCJG-AXDSSHIGSA-N 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 claims 1
- 102200027218 c.217G>A Human genes 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 239000011022 opal Substances 0.000 claims 1
- 102200033501 rs387907005 Human genes 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1419109.2A GB201419109D0 (en) | 2014-10-27 | 2014-10-27 | Incorporation of unnatural amino acids into proteins |
| GB1419109.2 | 2014-10-27 | ||
| PCT/GB2015/053141 WO2016066995A1 (en) | 2014-10-27 | 2015-10-21 | Incorporation of unnatural amino acids into proteins |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020177605A Division JP2021035374A (ja) | 2014-10-27 | 2020-10-22 | 非天然アミノ酸のタンパク質への組み込み |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017532043A JP2017532043A (ja) | 2017-11-02 |
| JP2017532043A5 true JP2017532043A5 (https=) | 2018-10-25 |
| JP6843044B2 JP6843044B2 (ja) | 2021-03-17 |
Family
ID=52103474
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017519813A Active JP6843044B2 (ja) | 2014-10-27 | 2015-10-21 | 非天然アミノ酸のタンパク質への組み込み |
| JP2020177605A Withdrawn JP2021035374A (ja) | 2014-10-27 | 2020-10-22 | 非天然アミノ酸のタンパク質への組み込み |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020177605A Withdrawn JP2021035374A (ja) | 2014-10-27 | 2020-10-22 | 非天然アミノ酸のタンパク質への組み込み |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10738339B2 (https=) |
| EP (1) | EP3212799B1 (https=) |
| JP (2) | JP6843044B2 (https=) |
| KR (1) | KR102516695B1 (https=) |
| CN (1) | CN107002088B (https=) |
| BR (1) | BR112017008108A2 (https=) |
| CA (1) | CA2965085A1 (https=) |
| GB (1) | GB201419109D0 (https=) |
| WO (1) | WO2016066995A1 (https=) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IN2014DN09922A (https=) | 2012-05-18 | 2015-08-14 | Medical Res Council | |
| GB2528227A (en) | 2014-03-14 | 2016-01-20 | Medical Res Council | Cyclopropene amino acids and methods |
| WO2018044143A1 (ko) * | 2016-09-05 | 2018-03-08 | 한국과학기술원 | 목적 단백질의 위치 특이적 변형이 시공간적으로 조절되는 마우스, 이의 제조방법 및 그 용도 |
| CN106834349B (zh) * | 2017-02-28 | 2019-12-13 | 中国人民解放军军事医学科学院 | 一种安全性提高的病毒基因递送系统 |
| EP3630977B1 (en) * | 2017-06-02 | 2024-02-21 | Ambrx, Inc. | Methods and compositions for promoting non-natural amino acid-containing protein production |
| KR20210005172A (ko) * | 2018-05-01 | 2021-01-13 | 암브룩스, 인코포레이티드 | 항체 발현을 최적화하는 방법 |
| CN112673109A (zh) * | 2018-09-07 | 2021-04-16 | 免疫医疗有限责任公司 | 细胞选择方法 |
| GB201817444D0 (en) | 2018-10-26 | 2018-12-12 | Res & Innovation Uk | Methods and compositions |
| EP3696189A1 (en) * | 2019-02-14 | 2020-08-19 | European Molecular Biology Laboratory | Means and methods for preparing engineered target proteins by genetic code expansion in a target protein selective manner |
| CN111718949B (zh) * | 2019-03-19 | 2021-10-01 | 宁波鲲鹏生物科技有限公司 | 利用双质粒系统在蛋白中引入非天然氨基酸 |
| CN111850020B (zh) * | 2019-04-25 | 2021-05-07 | 苏州鲲鹏生物技术有限公司 | 利用质粒系统在蛋白中引入非天然氨基酸 |
| CN111849929B (zh) * | 2019-04-30 | 2021-05-11 | 苏州鲲鹏生物技术有限公司 | 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 |
| GB201906775D0 (en) | 2019-05-14 | 2019-06-26 | Res & Innovation Uk | Synthetic genome |
| TW202128996A (zh) * | 2019-10-10 | 2021-08-01 | 美商史基普研究協會 | 用於活體內合成非天然多肽的組合物及方法 |
| CN117881779A (zh) | 2021-05-28 | 2024-04-12 | 英国研究与创新署 | 微生物及其用途 |
| CN113481239A (zh) * | 2021-07-01 | 2021-10-08 | 四川大学华西医院 | 一种通过Rosa26位点向细胞系中引入非天然氨基酸编码体系的方法及其细胞系 |
| WO2023282315A1 (ja) * | 2021-07-07 | 2023-01-12 | 味の素株式会社 | 非天然アミノ酸含有タンパク質の分泌生産法 |
| GB202110137D0 (en) | 2021-07-14 | 2021-08-25 | Res & Innovation Uk | Methods for optimising protein production |
| CN113699124B (zh) * | 2021-09-08 | 2022-04-12 | 北京大学 | 一种含非天然氨基酸蛋白的制备方法 |
| WO2023196596A2 (en) * | 2022-04-08 | 2023-10-12 | Absci Corporation | Trna synthetases and methods of use thereof |
| EP4689125A1 (en) * | 2023-03-30 | 2026-02-11 | The Chinese University of Hong Kong | Unnatural amino acid in cellulo synthesis for site-specific protein modification |
| CN120752342A (zh) * | 2023-07-13 | 2025-10-03 | 深圳华大生命科学研究院 | 工程生物遏制系统及方法 |
| CN120210144A (zh) * | 2023-12-25 | 2025-06-27 | 康码（上海）生物科技有限公司 | 一种改造的氨酰-tRNA合成酶、核酸构建物以及基因工程菌株 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2004321117B2 (en) * | 2003-12-18 | 2010-09-02 | The Scripps Research Institute | Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells |
| EP1807515A4 (en) * | 2004-10-27 | 2008-06-18 | Scripps Research Inst | Orthogonal translation components for the in vivo incorporation of unnatural amino acids |
| JP5590649B2 (ja) | 2007-09-20 | 2014-09-17 | 独立行政法人理化学研究所 | 変異体ピロリジル−ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法 |
| GB2470770A (en) | 2009-06-04 | 2010-12-08 | Medical Res Council | Incorporation of unnatural amino acids having an orthogonal functional group into polypeptides using orthogonal tRNA/tRNA synthetase pairs |
| US20120077186A1 (en) * | 2010-05-07 | 2012-03-29 | Oregon Health & Science University | Use of cysteine-derived suppressor trnas for non-native amino acid incorporation |
| GB201201100D0 (en) | 2012-01-20 | 2012-03-07 | Medical Res Council | Polypeptides and methods |
-
2014
- 2014-10-27 GB GBGB1419109.2A patent/GB201419109D0/en not_active Ceased
-
2015
- 2015-10-21 KR KR1020177014048A patent/KR102516695B1/ko active Active
- 2015-10-21 WO PCT/GB2015/053141 patent/WO2016066995A1/en not_active Ceased
- 2015-10-21 CA CA2965085A patent/CA2965085A1/en not_active Abandoned
- 2015-10-21 BR BR112017008108A patent/BR112017008108A2/pt not_active Application Discontinuation
- 2015-10-21 JP JP2017519813A patent/JP6843044B2/ja active Active
- 2015-10-21 CN CN201580058784.6A patent/CN107002088B/zh active Active
- 2015-10-21 US US15/521,103 patent/US10738339B2/en active Active
- 2015-10-21 EP EP15794622.9A patent/EP3212799B1/en active Active
-
2020
- 2020-10-22 JP JP2020177605A patent/JP2021035374A/ja not_active Withdrawn
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