JP2017531687A - 養子細胞免疫療法の有効性を増強させるための組成物および方法 - Google Patents
養子細胞免疫療法の有効性を増強させるための組成物および方法 Download PDFInfo
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Abstract
Description
本出願は、米国特許法§119(e)の下、2014年10月27日に出願された米国仮出願第62/069,168号(この出願は、その全体が参考として本明細書に援用される)に対する利益を主張する。
本発明は、National Institutes of Healthによって付与された助成金第CA114536号、同第AI053193号および同第P51−OD010425号の下、政府の支援を受けてなされた。政府は、本発明に一定の権利を有する。
本開示は、一般的に免疫療法の有効性を向上または増強させるための組成物および方法に関する。より具体的には、本開示は、がんなどの、標的化された抗原の発現と関連する疾患または障害を処置するために、抗原結合タンパク質(例えば、キメラ抗原受容体)を発現するT細胞を、養子細胞免疫療法を増強するための改変T細胞の抗原結合タンパク質により特異的に結合される抗原を発現するように改変された細胞(例えば、造血前駆細胞、改変ヒト免疫系細胞またはそれらの組合せ)と共に使用することに関する。
ある特定の態様では、本開示は、改変ヒト造血前駆細胞、改変ヒト免疫系細胞またはそれらの組合せの集団を含む養子細胞免疫療法組成物であって、改変細胞の第1の集団は、抗原結合タンパク質(例えば、CAR)をコードする核酸分子を含むT細胞であり、改変細胞の第2の集団は、抗原(例えば、がん特異的抗原、抗イディオタイプ抗体またはその結合断片)をコードする核酸分子を含む、養子細胞免疫療法組成物を提供する。これらの実施形態では、抗原結合タンパク質は、細胞外結合成分と細胞内エフェクター成分との間に位置する疎水性部分を含み、細胞外結合成分は、改変細胞の第2の集団によってコードされた抗原に特異的である。例えば、抗原結合タンパク質は、T細胞受容体(TCR)またはキメラ抗原受容体であり得る。
本明細書に記載するCARのような抗原結合タンパク質、抗原に特異的な高親和性組換えTCR、または抗原もしくは抗原特異的結合成分に対する抗イディオタイプ抗体をコードする単離もしくは組換え核酸分子は、分子生物学の様々な方法および技術またはポリペプチド精製技術により生成し、調製することができる。CARのような抗原結合タンパク質、抗原に特異的な高親和性組換えTCR、または抗原もしくは目的の抗原特異的結合成分に対する抗イディオタイプ抗体を組換えにより生成するために使用する発現ベクターの構築は、例えば、Sambrookら(1989年および2001年版;Molecular Cloning: A Laboratory Manual、Cold Spring Harbor Laboratory Press、NY)およびAusubelら(Current Protocols in Molecular Biology(2003年))に記載されているような、制限エンドヌクレアーゼ消化、ライゲーション、形質転換、プラスミド精製およびDNA配列決定の使用を含む、当技術分野で公知の任意の適切な分子生物学工学技術を使用することによって遂行することができる。効率的な転写および翻訳を得るために、各組換え発現構築物におけるポリヌクレオチドは、リーダー配列およびとりわけ免疫原をコードするヌクレオチド配列に作動可能に(すなわち、作動可能なように)連結したプロモーターなどの、少なくとも1つの適切な発現制御配列(調節配列とも呼ばれる)を含む。ある特定の実施形態では、ポリヌクレオチドは、標的宿主細胞における効率的な発現のために、コドンが最適化されている。
ある特定の態様では、本開示は、抗原結合タンパク質をコードする核酸分子を含む改変ヒトT細胞の集団を含む有効量の組成物をヒト被験体に投与することにより過剰増殖性障害または状態(例えば、抗原過剰発現によって特徴付けられる)を処置する方法を対象とするものであって、抗原結合タンパク質は、細胞外結合成分と細胞内エフェクター成分との間に位置する疎水性部分を含み、改変ヒト造血前駆細胞、改変ヒト免疫系細胞またはそれらの組合せの集団は、抗原結合タンパク質の細胞外結合成分によって特異的に認識される抗原をコードする核酸分子を含む。ある特定の実施形態では、投与ステップは、複数回で、数週間、数ヵ月または最長で2年もしくはそれ超の期間にわたり反復することができる。
養子移入免疫療法用のROR1 CAR−T細胞の特徴付け
ヒトおよびマカクの4−1BBおよびCD3ζシグナル伝達分子は、高度に保存されており(95%)、CD3ζの免疫受容体チロシンベースの活性化モチーフの100%の同一性が認められる。ROR1 CARがマカクT細胞において機能的であることを保証するために、T細胞にヒトまたはマカクの4−1BBおよびCD3ζシグナル伝達ドメインをコードするROR1 CARを形質導入し、ROR1+標的細胞の認識を評価した。形質導入T細胞を精製するための選択マーカーを提供するために、アカゲザルtCD19をコードする配列をT2Aリボソームスキップエレメントの下流のベクターに組み込んだ(Bergerら、J. Med. Primatol.、40巻、88頁、2011年)。ヒト4−1BB/CD3ζを含むROR1 CARまたはマカク4−1BB/CD3ζを含むROR1 CARを形質導入したマカクT細胞によるK562/ROR1腫瘍細胞の認識は、同等であった(図1A)。したがって、ヒトシグナル伝達ドメインを含む構築物を使用したin vivo安全性試験を使用して、このベクターの臨床への移行の可能性を評価した。
ROR1 CAR−T細胞はin vivoで機能する
自己ROR1 CAR−T細胞を安全に移入することができるかどうかを決定し、in vivoでのそれらの持続性および移動を解析するために、1×108個のROR1 CAR−T細胞/kg(1:1のCD4:CD8比)の用量を1匹のマカクに投与した。この細胞の用量は、マカクに安全に投与されたCMV特異的T細胞の用量より低いが、CD19+B細胞悪性疾患に対するCAR−T細胞療法の臨床試験(Mausら、Blood、123巻、2625頁、2014年)に使用された用量に等しいかまたは超えている。並行して、同じ用量のEGFRt+T細胞を、細胞の持続性および移動についての対照に投与した。動物を、T細胞の注入の後に発熱、呼吸困難、食欲、下痢および体重減少についてモニターし、注入前後の血液試料をCBC、血清化学およびサイトカインレベルについて検査した。即時または遅延型の臨床的異常は、この細胞用量では観察されなかった。T細胞の注入の前の動物の鎮静のための筋肉内(i.m.)ケタミン注射後にマカクで観察された筋肉由来のクレアチンホスホキナーゼ(CPK)の一過性の増加は別として、体重、CBCおよび血清化学は、正常限界内に留まっていた(図2A)。IFN−γおよびIL−6の血漿レベルがROR1 CAR−T細胞の後の1日目に増加したが、3日目までに正常に戻った(図2B)。IFN−γおよびIL−6の増加は、別の動物へのマーカー遺伝子のみを形質導入した自己T細胞の5倍高い細胞用量の移入後には観察されず(データは示さず)、これは、ROR1 CAR−T細胞を注入した後のサイトカインレベルの上昇がin vivoでのCAR−T細胞の一時的な活性化を反映していたことを示すものである。
ROR1 CAR−T細胞はin vivoで抗原に応答する
より高いCAR−T細胞用量の注入は、ROR1+悪性疾患を処置するために必要である可能性があり、とりわけ、CAR−T細胞が、高レベルのROR1を発現する腫瘍細胞の認識によりin vivoで活性化された場合に、正常組織に対する毒性を示す可能性がある。マカクにおけるB細胞上のROR1発現の解析中に、ヒトと異なり、一部の動物がROR1を発現するLNにおける高頻度の成熟B細胞を有することが観察された(データは示さず)。LNに高レベルのROR1+B細胞を有する2匹の動物をより高用量(5×108/kg)のROR1 CAR−T細胞の安全性の解析に選択した。なぜなら、最初の動物における経験で、ROR1+B細胞の減少がCAR−T細胞のin vivo機能の代用となることが示唆されたからである。CD34の濃縮がEGFRt選択より効率的であったため、tCD34をこれらの動物における対照T細胞におけるマーカー遺伝子として使用した。これにより、この実験に必要なより高いT細胞用量を得ることが促進された。in vivoでのROR1 CAR−T細胞の活性化が毒性を示す可能性を最大限にするために、毒性が以前に観察されなかった場合、CAR−T細胞の投与の5日後に細胞表面tROR1を発現するようにトランスフェクトした自己T細胞の注入を使用した(図4A)。
Claims (75)
- 養子細胞免疫療法を改善するための方法であって、前記方法は:
(a)抗原結合タンパク質をコードする核酸分子を含む改変ヒトT細胞の集団の有効量を被験体に投与するステップであって、前記抗原結合タンパク質は、細胞外結合成分と細胞内エフェクター成分との間に位置する疎水性部分を含む、ステップと、
(b)抗原をコードする核酸分子を含む改変ヒト造血前駆細胞、改変ヒト免疫系細胞またはそれらの組合せの集団の有効量を前記被験体に投与するステップであって、ステップ(a)の前記改変ヒトT細胞からの前記抗原結合タンパク質の前記細胞外結合成分が、このステップ(b)の改変細胞の前記集団によりコードされる前記抗原に特異的である、ステップと
を含み、
それにより、前記養子細胞免疫療法の有効性を増強、強化もしくは向上させる、方法。 - 被験体における疾患を処置するための方法であって、前記方法は:
(a)抗原結合タンパク質をコードする核酸分子を含む改変ヒトT細胞の集団の有効量を被験体に投与するステップであって、前記抗原結合タンパク質は、細胞外結合成分と細胞内エフェクター成分との間に位置する疎水性部分を含む、ステップと、
(b)抗原をコードする核酸分子を含む改変ヒト造血前駆細胞、改変ヒト免疫系細胞またはそれらの組合せの集団の有効量を前記被験体に投与するステップであって、ステップ(a)の前記改変ヒトT細胞からの前記抗原結合タンパク質の前記細胞外結合成分が、このステップ(b)の改変細胞の前記集団によりコードされる前記抗原に特異的である、ステップと
(c)任意選択でステップ(a)、ステップ(b)またはステップ(a)と(b)の両方を反復するステップと
を含み、
それにより、養子細胞免疫療法により疾患を処置する、方法。 - 前記疾患がウイルス性疾患、細菌性疾患、がん、炎症性疾患、免疫疾患または加齢性疾患である、請求項2に記載の方法。
- 過剰増殖性疾患を処置するための方法であって、前記方法は:
(a)抗原結合タンパク質をコードする核酸分子を含む改変ヒトT細胞の集団の有効量を被験体に投与するステップであって、前記抗原結合タンパク質は、細胞外結合成分と細胞内エフェクター成分との間に位置する疎水性部分を含む、ステップと、
(b)抗原をコードする核酸分子を含む改変ヒト造血前駆細胞、改変ヒト免疫系細胞またはそれらの組合せの集団の有効量を前記被験体に投与するステップであって、ステップ(a)の前記改変ヒトT細胞からの前記抗原結合タンパク質の前記細胞外結合成分が、このステップ(b)の改変細胞の前記集団によりコードされる前記抗原に特異的である、ステップと
(c)任意選択でステップ(a)、ステップ(b)またはステップ(a)と(b)の両方を反復するステップと
を含み、
それにより、前記過剰増殖性疾患を処置する、方法。 - 前記過剰増殖性疾患が血液学的悪性疾患または固形がんである、請求項4に記載の方法。
- 前記血液学的悪性疾患が、急性リンパ芽球性白血病(ALL)、急性骨髄性白血病(AML)、慢性骨髄性白血病(CML)、慢性好酸球性白血病(CEL)、骨髄異形成症候群(MDS)、非ホジキンリンパ腫(NHL)または多発性骨髄腫(MM)から選択される、請求項5に記載の方法。
- 前記固形がんが、胆管がん、膀胱がん、骨および軟部組織癌腫、脳腫瘍、乳がん、子宮頸がん、結腸がん、結腸直腸腺癌、結腸直腸がん、類腱腫、胚性がん、子宮内膜がん、食道がん、胃がん、胃腺癌、多形膠芽腫、婦人科腫瘍、頭頸部扁平上皮癌、肝臓がん、肺がん、悪性黒色腫、骨肉腫、卵巣がん、膵臓がん、膵管腺癌、原発性星状細胞腫瘍、原発性甲状腺がん、前立腺がん、腎臓がん、腎細胞癌、横紋筋肉腫、皮膚がん、軟部組織肉腫、精巣胚細胞腫瘍、尿路上皮がん、子宮肉腫または子宮がんから選択される、請求項5に記載の方法。
- 前記結合成分が、抗体可変断片(Fv)、TCR可変ドメイン、受容体エクトドメインまたはリガンドである、請求項1から7のいずれか一項に記載の方法。
- 前記結合成分が、可変領域リンカーを含むscFvまたはscTCRである、請求項8に記載の方法。
- 前記可変領域リンカーが(GlyxSery)nを含み、式中、xおよびyは、独立に1〜5の整数であり、nは、1〜10の整数である、請求項9に記載の方法。
- 前記疎水性部分が膜貫通ドメインである、請求項1から10のいずれか一項に記載の方法。
- 前記膜貫通ドメインがCD4、CD8、CD28またはCD27膜貫通ドメインである、請求項11に記載の方法。
- 前記細胞内エフェクター成分が、CD3ε、CD3δ、CD3ζ、CD25、CD27、CD28、CD79A、CD79B、CD134、CD137、CARD11、DAP10、FcRα、FcRβ、FcRγ、Fyn、HVEM、ICOS、Lck、LAG3、LAT、LRP、NKG2D、NOTCH1、NOTCH2、NOTCH3、NOTCH4、ROR2、Ryk、SLAMF1、Slp76、pTα、TCRα、TCRβ、TRIM、Zap70、PTCH2またはそれらの任意の組合せの細胞内領域を含む、請求項1から12のいずれか一項に記載の方法。
- 前記細胞内エフェクター成分が、CD3ζならびにCD27、CD28、CD134およびCD137のうちの1つまたは複数を含む、請求項1から13のいずれか一項に記載の方法。
- 前記細胞内エフェクター成分が、LRP、NOTCH1、NOTCH2、NOTCH3、NOTCH4、ROR2またはRykを含む、請求項1から13のいずれか一項に記載の方法。
- 前記結合成分が、α−フェトプロテイン(AFP)、B7H4、BTLA、CD3、CD19、CD20、CD25、CD22、CD28、CD30、CD40、CD44v6、CD52、CD56、CD79b、CD80、CD81、CD86、CD134(OX40)、CD137(4−1BB)、CD151、CD276、CA125、CEA、CEACAM6、c−Met、CT−7、CTLA−4、EGFR、EGFRvIII、ErbB2、ErbB3、ErbB4、EphA2、FLT1、FLT4、Frizzled、O−アセチル−GD2、GD2、GHRHR、GHR、GITR、gp130、HVEM、IGF1R、IL6R、KDR、L1CAM、Lewis A、Lewis Y、LTβR、LIFRβ、LRP5、MAGE、メソセリン、MUC1、NY−ESO−1、がん特異的新抗原、OSMRβ、PD1、PD−L1、PD−L2、PSMA、PTCH1、RANK、Robo1、ROR1、TERT、TGFBR2、TGFBR1、TLR7、TLR9、TNFRSF4、TNFR1、TNFR2、チロシナーゼ、TWEAK−RまたはWT−1に特異的である、請求項1から15のいずれか一項に記載の方法。
- 前記抗原結合タンパク質がT細胞受容体(TCR)またはキメラ抗原受容体である、請求項1から16のいずれか一項に記載の方法。
- 前記免疫系細胞が、CD4+T細胞、CD8+T細胞、CD4−CD8−二重陰性T細胞、γδT細胞、ナチュラルキラー細胞、樹状細胞またはそれらの任意の組合せである、請求項1から17のいずれか一項に記載の方法。
- 前記改変T細胞がナイーブT細胞、セントラルメモリーT細胞、エフェクターメモリーT細胞またはそれらの任意の組合せである、請求項1から18のいずれか一項に記載の方法。
- 改変T細胞の第1の集団が、CD4+T細胞、CD8+T細胞またはCD4+とCD8+T細胞の両方から本質的になる、請求項1から19のいずれか一項に記載の方法。
- 改変細胞の第2の集団が改変ヒト造血前駆細胞を含む、請求項1から20のいずれか一項に記載の方法。
- 改変細胞の第2の集団が改変ヒト免疫系細胞を含む、請求項1から20のいずれか一項に記載の方法。
- 前記改変免疫系細胞が、CD4+T細胞、CD8+T細胞またはCD4+とCD8+T細胞の両方から本質的になる、請求項22に記載の方法。
- 前記細胞がex vivoで組換えにより改変される、請求項1から23のいずれか一項に記載の方法。
- 前記ex vivo改変細胞がウイルスベクターを使用して改変される、請求項24に記載の方法。
- 前記ウイルスベクターがレンチウイルスベクターまたはγ−レトロウイルスベクターである、請求項25に記載の方法。
- 改変細胞の前記集団が同種異系、同系または自己のものである、請求項1から26のいずれか一項に記載の方法。
- 前記改変ヒトT細胞からの前記抗原結合タンパク質の前記細胞外結合成分が、前記抗原を過剰発現する細胞に対して向けられている、請求項1から27のいずれか一項に記載の方法。
- 前記改変細胞が静脈内投与される、請求項1から28のいずれか一項に記載の方法。
- ステップ(a)の前記改変T細胞の複数回用量、ステップ(b)の改変細胞の複数回用量、またはそれらの組合せを前記被験体に投与するステップを含む、請求項1から29のいずれか一項に記載の方法。
- ステップ(a)の改変T細胞の前記複数回用量が、約1週間から約4週間までの投与の間の間隔で投与される、請求項30に記載の方法。
- ステップ(a)の前記改変T細胞が、ステップ(b)の前記改変細胞と同時にまたは逐次的に投与される、請求項1から31のいずれか一項に記載の方法。
- ステップ(b)の改変細胞の初回用量が、ステップ(a)の前記改変T細胞を投与して約1日から約28日後までに投与される、請求項32に記載の方法。
- ステップ(b)の前記改変細胞が、ステップ(a)の前記改変T細胞を投与してから約24時間以内に逐次的に投与されるか、またはステップ(a)の前記改変T細胞が、ステップ(b)の前記改変細胞を投与してから約24時間以内に逐次的に投与される、請求項32に記載の方法。
- 初回の投与がステップ(a)の前記改変T細胞とステップ(b)の前記改変細胞との混合物を含む組成物を投与することを含む、請求項32に記載の方法。
- ステップ(b)の前記改変細胞が、ステップ(a)の前記改変T細胞を投与した後約365日間まで、1つまたは複数の間隔で1回または複数回用量でさらに投与される、請求項32から35のいずれか一項に記載の方法。
- ステップ(b)の前記改変細胞が改変造血前駆細胞である、請求項32から36のいずれか一項に記載の方法。
- ステップ(b)の前記改変細胞が改変T細胞である、請求項32から36のいずれか一項に記載の方法。
- ステップ(a)の前記改変T細胞が、前記被験体に約106細胞/m2〜約1011細胞/m2の用量で投与され、ステップ(b)の前記改変T細胞が、前記被験体に約106細胞/m2〜約1011細胞/m2の用量で投与される、請求項1から38のいずれか一項に記載の方法。
- サイトカインを投与するステップをさらに含む、請求項1から39のいずれか一項に記載の方法。
- 前記サイトカインが、IL−2、IL−15、IL−21またはそれらの任意の組合せである、請求項40に記載の方法。
- 前記サイトカインがIL−2であり、ステップ(a)の前記改変T細胞と同時にまたは逐次的に投与される、請求項41に記載の方法。
- 前記被験体にサイトカインの投与前にステップ(a)の前記改変T細胞が少なくとも3または4回投与された場合、前記サイトカインが逐次的に投与される、請求項42に記載の方法。
- 前記サイトカインがIL−2であり、IL−2を皮下投与する、請求項40から43のいずれか一項に記載の方法。
- 前記被験体が免疫抑制療法をさらに受けている、請求項1から44のいずれか一項に記載の方法。
- 前記免疫抑制療法が、カルシニューリン阻害剤、コルチコステロイド、微小管阻害剤、低用量のミコフェノール酸プロドラッグまたはそれらの任意の組合せから選択される、請求項45に記載の方法。
- 前記被験体が非骨髄破壊的または骨髄破壊的造血細胞移植を受けた、請求項1から46のいずれか一項に記載の方法。
- 前記造血細胞移植が自己のものである、請求項47に記載の方法。
- 前記造血細胞移植が同種異系のものである、請求項47に記載の方法。
- 前記非骨髄破壊的造血細胞移植の少なくとも3ヵ月後にまたは前記骨髄破壊的造血細胞移植の少なくとも2ヵ月後に、ステップ(a)の前記改変T細胞および/またはステップ(b)の前記改変細胞を前記被験体に投与する、請求項47から49のいずれか一項に記載の方法。
- 改変ヒト造血前駆細胞、改変ヒト免疫系細胞またはそれらの組合せの集団を含む養子細胞免疫療法組成物であって、改変細胞の第1の集団は、抗原結合タンパク質をコードする核酸分子を含むT細胞であり、改変細胞の第2の集団は、抗原をコードする核酸分子を含み、
前記抗原結合タンパク質は、細胞外結合成分と細胞内エフェクター成分との間に位置する疎水性部分を含み、
前記細胞外結合成分は、改変細胞の前記第2の集団によりコードされる前記抗原に特異的である、養子細胞免疫療法組成物。 - 前記結合成分が、抗体可変断片(Fv)、TCR可変ドメイン、受容体エクトドメインまたはリガンドである、請求項51に記載の組成物。
- 前記結合成分が、可変領域リンカーを含むscFvまたはscTCRである、請求項52に記載の組成物。
- 前記可変領域リンカーが(GlyxSery)nを含み、式中、xおよびyは、独立に1〜5の整数であり、nは、1〜10の整数である、請求項53に記載の組成物。
- 前記疎水性部分が膜貫通ドメインである、請求項51から54のいずれか一項に記載の組成物。
- 前記膜貫通ドメインが、CD4、CD8、CD28またはCD27膜貫通ドメインである、請求項55に記載の組成物。
- 前記細胞内エフェクター成分が、CD3ε、CD3δ、CD3ζ、CD25、CD27、CD28、CD79A、CD79B、CD134、CD137、CARD11、DAP10、FcRα、FcRβ、FcRγ、Fyn、HVEM、ICOS、Lck、LAG3、LAT、LRP、NKG2D、NOTCH1、NOTCH2、NOTCH3、NOTCH4、ROR2、Ryk、SLAMF1、Slp76、pTα、TCRα、TCRβ、TRIM、Zap70、PTCH2またはそれらの任意の組合せの細胞内領域を含む、請求項51から56のいずれか一項に記載の組成物。
- 前記細胞内エフェクター成分が、CD3ζならびにCD27、CD28、CD134およびCD137のうちの1つまたは複数を含む、請求項51から57のいずれか一項に記載の組成物。
- 前記細胞内エフェクター成分が、LRP、NOTCH1、NOTCH2、NOTCH3、NOTCH4、ROR2またはRykを含む、請求項51から57のいずれか一項に記載の組成物。
- 前記結合成分が、α−フェトプロテイン(AFP)、B7H4、BTLA、CD3、CD19、CD20、CD25、CD22、CD28、CD30、CD40、CD44v6、CD52、CD56、CD79b、CD80、CD81、CD86、CD134(OX40)、CD137(4−1BB)、CD151、CD276、CA125、CEA、CEACAM6、c−Met、CT−7、CTLA−4、EGFR、EGFRvIII、ErbB2、ErbB3、ErbB4、EphA2、FLT1、FLT4、Frizzled、O−アセチル−GD2、GD2、GHRHR、GHR、GITR、gp130、HVEM、IGF1R、IL6R、KDR、L1CAM、Lewis A、Lewis Y、LTβR、LIFRβ、LRP5、MAGE、メソセリン、MUC1、NY−ESO−1、がん特異的新抗原、OSMRβ、PD1、PD−L1、PD−L2、PSMA、PTCH1、RANK、Robo1、ROR1、TERT、TGFBR2、TGFBR1、TLR7、TLR9、TNFRSF4、TNFR1、TNFR2、チロシナーゼ、TWEAK−RまたはWT−1に特異的である、請求項51から59のいずれか一項に記載の組成物。
- 前記抗原結合タンパク質がT細胞受容体(TCR)またはキメラ抗原受容体である、請求項51から60のいずれか一項に記載の組成物。
- 前記免疫系細胞が、CD4+T細胞、CD8+T細胞、CD4−CD8−二重陰性T細胞、γδT細胞、ナチュラルキラー細胞、樹状細胞またはそれらの任意の組合せである、請求項51から61のいずれか一項に記載の組成物。
- 前記T細胞が、ナイーブT細胞、セントラルメモリーT細胞、エフェクターメモリーT細胞またはそれらの任意の組合せである、請求項51から62のいずれか一項に記載の組成物。
- 改変T細胞の前記第1の集団が、CD4+T細胞、CD8+T細胞またはCD4+とCD8+T細胞の両方から本質的になる、請求項51から63のいずれか一項に記載の組成物。
- 改変細胞の前記第2の集団が改変ヒト造血前駆細胞を含む、請求項51から64のいずれか一項に記載の組成物。
- 改変細胞の前記第2の集団が改変ヒト免疫系細胞を含む、請求項51から64のいずれか一項に記載の組成物。
- 前記改変ヒト免疫系細胞が、CD4+T細胞、CD8+T細胞またはCD4+とCD8+T細胞の両方から本質的になる、請求項66に記載の組成物。
- 前記細胞がex vivoで組換えにより改変される、請求項51から67のいずれか一項に記載の組成物。
- 前記ex vivo改変細胞がウイルスベクターを使用して改変される、請求項68に記載の組成物。
- 前記ウイルスベクターがレンチウイルスベクターまたはγ−レトロウイルスベクターである、請求項69に記載の組成物。
- 改変細胞の前記集団が同種異系、同系または自己のものである、請求項51から70のいずれか一項に記載の組成物。
- 薬学的に許容される担体、希釈剤または賦形剤をさらに含む、請求項51から71のいずれか一項に記載の養子細胞免疫療法組成物。
- 請求項51から72のいずれか一項に記載の組成物を含む単位用量形態。
- 請求項1から50のいずれか一項に記載される、ステップ(a)の改変T細胞を含む単位用量形態およびステップ(b)の改変細胞を含む単位用量形態。
- ステップ(b)の前記改変細胞が改変ヒト造血前駆細胞または改変ヒトT細胞である、請求項66に記載の単位用量形態。
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ES2897731T3 (es) | 2022-03-02 |
CA2965347A1 (en) | 2016-05-06 |
KR20170068600A (ko) | 2017-06-19 |
CN107106611B (zh) | 2021-11-23 |
CN107106611A (zh) | 2017-08-29 |
US10828352B2 (en) | 2020-11-10 |
BR122021000068A8 (pt) | 2023-05-02 |
US20170246279A1 (en) | 2017-08-31 |
NZ731279A (en) | 2021-12-24 |
CA2965347C (en) | 2023-03-14 |
RU2017114174A3 (ja) | 2019-05-07 |
BR112017008710A2 (pt) | 2017-12-19 |
AU2015339402B2 (en) | 2021-08-26 |
HK1243631A1 (zh) | 2018-07-20 |
JP7005346B2 (ja) | 2022-02-04 |
WO2016069647A1 (en) | 2016-05-06 |
EP3212223B1 (en) | 2021-08-18 |
RU2716716C2 (ru) | 2020-03-16 |
BR122021000068A2 (ja) | 2017-12-19 |
BR112017008710A8 (pt) | 2023-04-25 |
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