JP2017528142A5 - - Google Patents

Info

Publication number

JP2017528142A5

JP2017528142A5 JP2017514421A JP2017514421A JP2017528142A5 JP 2017528142 A5 JP2017528142 A5 JP 2017528142A5 JP 2017514421 A JP2017514421 A JP 2017514421A JP 2017514421 A JP2017514421 A JP 2017514421A JP 2017528142 A5 JP2017528142 A5 JP 2017528142A5

Authority

JP

Japan

Prior art keywords

mammalian

scnt

embryo

oocyte

cell

Prior art date

2015-09-15

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 238000010374 somatic cell nuclear transfer Methods 0.000 claims 43
• 210000001161 mammalian embryo Anatomy 0.000 claims 25
• 210000000287 oocyte Anatomy 0.000 claims 24
• 238000000034 method Methods 0.000 claims 23
• 239000003795 chemical substances by application Substances 0.000 claims 18
• 210000002257 embryonic structure Anatomy 0.000 claims 10
• 210000004962 mammalian cell Anatomy 0.000 claims 10
• 210000002459 blastocyst Anatomy 0.000 claims 6
• 210000004027 cell Anatomy 0.000 claims 6
• 230000000694 effects Effects 0.000 claims 5
• 102000008157 Histone Demethylases Human genes 0.000 claims 4
• 108010074870 Histone Demethylases Proteins 0.000 claims 4
• 102000016397 Methyltransferase Human genes 0.000 claims 4
• 108060004795 Methyltransferase Proteins 0.000 claims 4
• 230000004913 activation Effects 0.000 claims 3
• 239000012634 fragment Substances 0.000 claims 3
• 239000003112 inhibitor Substances 0.000 claims 3
• 150000007523 nucleic acids Chemical group 0.000 claims 3
• 108091033409 CRISPR Proteins 0.000 claims 2
• 101100287812 Caenorhabditis elegans jmjd-2 gene Proteins 0.000 claims 2
• 101150032905 Kdm4d gene Proteins 0.000 claims 2
• 241000124008 Mammalia Species 0.000 claims 2
• 108091030071 RNAI Proteins 0.000 claims 2
• 238000012258 culturing Methods 0.000 claims 2
• 230000009368 gene silencing by RNA Effects 0.000 claims 2
• 230000011987 methylation Effects 0.000 claims 2
• 238000007069 methylation reaction Methods 0.000 claims 2
• 108020004707 nucleic acids Proteins 0.000 claims 2
• 102000039446 nucleic acids Human genes 0.000 claims 2
• 108091023037 Aptamer Proteins 0.000 claims 1
• 238000010354 CRISPR gene editing Methods 0.000 claims 1
• 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
• 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
• 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
• 108091034117 Oligonucleotide Proteins 0.000 claims 1
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
• 230000003213 activating effect Effects 0.000 claims 1
• 238000002425 crystallisation Methods 0.000 claims 1
• 230000008025 crystallization Effects 0.000 claims 1
• 210000000805 cytoplasm Anatomy 0.000 claims 1
• 210000001671 embryonic stem cell Anatomy 0.000 claims 1
• 238000000338 in vitro Methods 0.000 claims 1
• 230000002401 inhibitory effect Effects 0.000 claims 1
• 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 1
• 210000000472 morula Anatomy 0.000 claims 1
• 230000003472 neutralizing effect Effects 0.000 claims 1
• 210000003101 oviduct Anatomy 0.000 claims 1
• 108090000765 processed proteins & peptides Proteins 0.000 claims 1
• 229940121649 protein inhibitor Drugs 0.000 claims 1
• 239000012268 protein inhibitor Substances 0.000 claims 1
• 150000003384 small molecules Chemical class 0.000 claims 1
• 210000000130 stem cell Anatomy 0.000 claims 1