JP2017507644A5 - - Google Patents

Info

Publication number

JP2017507644A5

JP2017507644A5 JP2016531634A JP2016531634A JP2017507644A5 JP 2017507644 A5 JP2017507644 A5 JP 2017507644A5 JP 2016531634 A JP2016531634 A JP 2016531634A JP 2016531634 A JP2016531634 A JP 2016531634A JP 2017507644 A5 JP2017507644 A5 JP 2017507644A5

Authority

JP

Japan

Prior art keywords

amino acid

polypeptide

seq

variant

lactase

Prior art date

2015-01-13

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 229920001184 polypeptide Polymers 0.000 claims 29
• 102000004196 processed proteins & peptides Human genes 0.000 claims 29
• 108090000765 processed proteins & peptides Proteins 0.000 claims 29
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 17
• 102100026189 Beta-galactosidase Human genes 0.000 claims 16
• 108010059881 Lactase Proteins 0.000 claims 16
• 108010005774 beta-Galactosidase Proteins 0.000 claims 16
• 229940116108 lactase Drugs 0.000 claims 16
• 238000006467 substitution reaction Methods 0.000 claims 14
• 125000003275 alpha amino acid group Chemical group 0.000 claims 13
• 230000000694 effects Effects 0.000 claims 12
• 150000001413 amino acids Chemical class 0.000 claims 7
• 125000000539 amino acid group Chemical class 0.000 claims 6
• 238000004519 manufacturing process Methods 0.000 claims 5
• 235000013365 dairy product Nutrition 0.000 claims 4
• 150000007523 nucleic acids Chemical group 0.000 claims 4
• GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims 3
• 239000008101 lactose Substances 0.000 claims 3
• 238000000034 method Methods 0.000 claims 3
• 239000000203 mixture Substances 0.000 claims 3
• 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
• 239000013604 expression vector Substances 0.000 claims 2
• 239000008267 milk Substances 0.000 claims 2
• 235000013336 milk Nutrition 0.000 claims 2
• 210000004080 milk Anatomy 0.000 claims 2
• 108020004707 nucleic acids Proteins 0.000 claims 2
• 102000039446 nucleic acids Human genes 0.000 claims 2
• KUWPCJHYPSUOFW-YBXAARCKSA-N 2-nitrophenyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1[N+]([O-])=O KUWPCJHYPSUOFW-YBXAARCKSA-N 0.000 claims 1
• 108090000790 Enzymes Proteins 0.000 claims 1
• 102000004190 Enzymes Human genes 0.000 claims 1
• -1 More preferably Chemical compound 0.000 claims 1
• 238000012258 culturing Methods 0.000 claims 1
• 229940088598 enzyme Drugs 0.000 claims 1
• 229930182830 galactose Natural products 0.000 claims 1
• 230000005764 inhibitory process Effects 0.000 claims 1
• 229910021645 metal ion Inorganic materials 0.000 claims 1
• 229920005862 polyol Polymers 0.000 claims 1
• 150000003077 polyols Chemical class 0.000 claims 1
• 238000002360 preparation method Methods 0.000 claims 1
• 239000003755 preservative agent Substances 0.000 claims 1
• 238000003259 recombinant expression Methods 0.000 claims 1
• 150000003839 salts Chemical class 0.000 claims 1