AU2015206094B2 - Improved enzyme variants of lactase from Kluyveromyces lactis - Google Patents

Improved enzyme variants of lactase from Kluyveromyces lactis Download PDF

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Publication number
AU2015206094B2
AU2015206094B2 AU2015206094A AU2015206094A AU2015206094B2 AU 2015206094 B2 AU2015206094 B2 AU 2015206094B2 AU 2015206094 A AU2015206094 A AU 2015206094A AU 2015206094 A AU2015206094 A AU 2015206094A AU 2015206094 B2 AU2015206094 B2 AU 2015206094B2
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Australia
Prior art keywords
variant
lactase
seq
polypeptide
amino acid
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AU2015206094A
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AU2015206094A1 (en
Inventor
Rene Marcel De Jong
Petrus Jacobus Theodorus Dekker
Ulrike Maria Muller
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DSM IP Assets BV
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DSM IP Assets BV
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/1526—Amino acids; Peptides; Protein hydrolysates; Nucleic acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01107—Protein-glucosylgalactosylhydroxylysine glucosidase (3.2.1.107)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01108—Lactase (3.2.1.108)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Dairy Products (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2015206094A 2014-01-14 2015-01-13 Improved enzyme variants of lactase from Kluyveromyces lactis Active AU2015206094B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP14151124 2014-01-14
EP14151124.6 2014-01-14
EP14169816 2014-05-26
EP14169816.7 2014-05-26
PCT/EP2015/050503 WO2015107050A1 (en) 2014-01-14 2015-01-13 Improved enzyme variants of lactase from kluyveromyces lactis

Publications (2)

Publication Number Publication Date
AU2015206094A1 AU2015206094A1 (en) 2016-06-09
AU2015206094B2 true AU2015206094B2 (en) 2020-09-10

Family

ID=52350110

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2015206094A Active AU2015206094B2 (en) 2014-01-14 2015-01-13 Improved enzyme variants of lactase from Kluyveromyces lactis

Country Status (10)

Country Link
US (2) US9994837B2 (enExample)
EP (2) EP3339433A1 (enExample)
JP (1) JP6632526B2 (enExample)
CN (1) CN105899659B (enExample)
AU (1) AU2015206094B2 (enExample)
BR (1) BR112016016342B1 (enExample)
DK (1) DK3094723T3 (enExample)
ES (1) ES2670422T3 (enExample)
MX (1) MX375778B (enExample)
WO (1) WO2015107050A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102533803B1 (ko) * 2017-04-07 2023-05-18 듀폰 뉴트리션 바이오사이언시즈 에이피에스 p-니트로벤질에스터라제 부활성의 부재 하에 β-갈락토시다제 및 락타제를 생산하는 바실러스 숙주 세포
AU2018251957C9 (en) 2017-04-11 2025-03-20 Kerry Group Services International Ltd Lactase enzymes with improved properties
AU2018250886C1 (en) * 2017-04-11 2023-10-19 Kerry Group Services International Ltd Lactase enzymes with improved activity at low temperatures
US12435326B2 (en) 2017-04-11 2025-10-07 Kerry Group Services International Ltd Lactase enzymes with improved properties
EP3609908B1 (en) * 2017-04-11 2025-10-15 Kerry Group Services International Ltd Lactase enzymes with improved properties
CN107523595A (zh) * 2017-08-21 2017-12-29 天津大学 一种高纯度低聚半乳糖的绿色制备方法
CN108102936B (zh) * 2018-02-26 2020-12-22 董颖军 乳酸克鲁维酵母突变株及其糖苷酶和应用
JP7502296B2 (ja) * 2018-12-03 2024-06-18 デュポン ニュートリション バイオサイエンシス エーピーエス 高ｇｏｓ繊維レベル及び低ラクトースレベルを提供するためのラクターゼの使用
CN110616228B (zh) * 2019-10-22 2023-07-04 怀化学院 一种高活力乳糖酶基因及其重组蛋白的制备方法与应用
CN110951630B (zh) * 2019-12-10 2021-06-18 刘建蔚 乳酸克鲁维酵母突变株xt82及其应用
EP4110909A1 (en) * 2020-02-26 2023-01-04 Novozymes A/S Polypeptide variants
CN114806906B (zh) * 2022-05-31 2023-02-21 江南大学 一株产中性乳糖酶的乳酸克鲁维酵母
CN118931882A (zh) * 2023-05-11 2024-11-12 山东恒鲁生物科技有限公司 一种改良的成熟多肽

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012175760A1 (es) * 2011-06-24 2012-12-27 Queizuar, S.L. Cepa de levadura kluyveromyces lactis y procedimientos de obtención de azúcares, etanol, beta-galactosidasa y biomasa

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US5492813A (en) * 1993-11-01 1996-02-20 Boehringer Mannheim Corporation Muteins of β-galactosidase fragments having increased activity
DK1261697T3 (da) * 1999-07-09 2004-11-22 Univ Liege Kuldeaktiv beta-galactosidase, fremgangsmåde til fremstilling heraf og anvendelsen deraf
JP5042431B2 (ja) * 2000-05-26 2012-10-03 アルラ・フーズ・エイ・エム・ビィ・エイ Ｂｉｆｉｄｏｂａｃｔｅｒｉｕｍから単離された新たな酵素
WO2004074468A1 (en) 2002-05-30 2004-09-02 Dsm Ip Assets B.V. Novel pectinases and uses thereof
NZ568554A (en) * 2005-11-28 2011-12-22 Dsm Ip Assets Bv Lactase enzyme preparations yielding a clean taste
WO2009009142A2 (en) * 2007-07-10 2009-01-15 Monsanto Technology, Llc Transgenic plants with enhanced agronomic traits
CN102250856B (zh) * 2011-06-23 2013-09-18 江南大学 一种耐热β-半乳糖苷酶突变体的构建

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012175760A1 (es) * 2011-06-24 2012-12-27 Queizuar, S.L. Cepa de levadura kluyveromyces lactis y procedimientos de obtención de azúcares, etanol, beta-galactosidasa y biomasa

Non-Patent Citations (3)

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Title
"SubName: Full=Beta-galactosidase;", UniProt, (2012-07-11), Database accession no. I2JQJ6, URL: EBI *
MAHONEY R R ET AL, "THERMOSTABILITY OF YEAST LACTASE KLUYVEROMYCES-MARXIANUS IN MILK", JOURNAL OF DAIRY RESEARCH, 1988, vol. 55, no. 3, pages 423 - 433 *
SUNIL BANSAL ET AL, "Production of β-galactosidase by Kluyveromyces marxianus MTCC 1388 using whey and effect of four different methods of enzyme extraction on β-galactosidase activity", Ind J Microbiol, 2008. vol. 48, no. 3, pp 337-341 *

Also Published As

Publication number Publication date
BR112016016342A2 (pt) 2017-10-03
CN105899659A (zh) 2016-08-24
US10612013B2 (en) 2020-04-07
ES2670422T3 (es) 2018-05-30
WO2015107050A1 (en) 2015-07-23
US9994837B2 (en) 2018-06-12
BR112016016342B1 (pt) 2023-05-09
CN105899659B (zh) 2025-06-17
EP3094723B1 (en) 2018-02-28
EP3094723A1 (en) 2016-11-23
EP3339433A1 (en) 2018-06-27
JP2017507644A (ja) 2017-03-23
MX375778B (es) 2025-03-07
DK3094723T3 (en) 2018-06-06
US20160333331A1 (en) 2016-11-17
AU2015206094A1 (en) 2016-06-09
JP6632526B2 (ja) 2020-01-22
US20180320156A1 (en) 2018-11-08
NZ720283A (en) 2022-03-25
MX2016009149A (es) 2016-09-09

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