ES2670422T3 - Variantes enzimáticas mejoradas de lactasa de Kluyveromyces lactis - Google Patents

Variantes enzimáticas mejoradas de lactasa de Kluyveromyces lactis Download PDF

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Publication number
ES2670422T3
ES2670422T3 ES15700373.2T ES15700373T ES2670422T3 ES 2670422 T3 ES2670422 T3 ES 2670422T3 ES 15700373 T ES15700373 T ES 15700373T ES 2670422 T3 ES2670422 T3 ES 2670422T3
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ES
Spain
Prior art keywords
variant
lactase
seq
amino acid
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES15700373.2T
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English (en)
Spanish (es)
Inventor
DE René Marcel JONG
Ulrike Maria MÜLLER
Petrus Jacobus Theodorus Dekker
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DSM IP Assets BV
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DSM IP Assets BV
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Publication of ES2670422T3 publication Critical patent/ES2670422T3/es
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/1526—Amino acids; Peptides; Protein hydrolysates; Nucleic acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01107—Protein-glucosylgalactosylhydroxylysine glucosidase (3.2.1.107)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01108—Lactase (3.2.1.108)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Dairy Products (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES15700373.2T 2014-01-14 2015-01-13 Variantes enzimáticas mejoradas de lactasa de Kluyveromyces lactis Active ES2670422T3 (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP14151124 2014-01-14
EP14151124 2014-01-14
EP14169816 2014-05-26
EP14169816 2014-05-26
PCT/EP2015/050503 WO2015107050A1 (en) 2014-01-14 2015-01-13 Improved enzyme variants of lactase from kluyveromyces lactis

Publications (1)

Publication Number Publication Date
ES2670422T3 true ES2670422T3 (es) 2018-05-30

Family

ID=52350110

Family Applications (1)

Application Number Title Priority Date Filing Date
ES15700373.2T Active ES2670422T3 (es) 2014-01-14 2015-01-13 Variantes enzimáticas mejoradas de lactasa de Kluyveromyces lactis

Country Status (10)

Country Link
US (2) US9994837B2 (enExample)
EP (2) EP3339433A1 (enExample)
JP (1) JP6632526B2 (enExample)
CN (1) CN105899659B (enExample)
AU (1) AU2015206094B2 (enExample)
BR (1) BR112016016342B1 (enExample)
DK (1) DK3094723T3 (enExample)
ES (1) ES2670422T3 (enExample)
MX (1) MX375778B (enExample)
WO (1) WO2015107050A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI3607097T3 (fi) * 2017-04-07 2023-09-11 Dupont Nutrition Biosci Aps BEETA-GALAKTOSIDAASEJA JA LAKTAASEJA ILMAN p-NITROBENTSYYLIESTERASISIVUAKTIIVISUUTTA TUOTTAVIA BACILLUS-ISÄNTÄSOLUJA
DK3609909T3 (da) 2017-04-11 2025-09-01 Kerry Group Services Int Ltd Laktaseenzymer med forbedret aktivitet ved lave temperaturer
WO2018189238A1 (en) * 2017-04-11 2018-10-18 Chr. Hansen A/S Lactase enzymes with improved properties
EP4011906A3 (en) 2017-04-11 2022-09-07 Chr. Hansen A/S Lactase enzymes with improved properties
US12435326B2 (en) 2017-04-11 2025-10-07 Kerry Group Services International Ltd Lactase enzymes with improved properties
CN107523595A (zh) * 2017-08-21 2017-12-29 天津大学 一种高纯度低聚半乳糖的绿色制备方法
CN108102936B (zh) * 2018-02-26 2020-12-22 董颖军 乳酸克鲁维酵母突变株及其糖苷酶和应用
JP7502296B2 (ja) * 2018-12-03 2024-06-18 デュポン ニュートリション バイオサイエンシス エーピーエス 高ｇｏｓ繊維レベル及び低ラクトースレベルを提供するためのラクターゼの使用
CN110616228B (zh) * 2019-10-22 2023-07-04 怀化学院 一种高活力乳糖酶基因及其重组蛋白的制备方法与应用
CN110951630B (zh) * 2019-12-10 2021-06-18 刘建蔚 乳酸克鲁维酵母突变株xt82及其应用
EP4110909A1 (en) * 2020-02-26 2023-01-04 Novozymes A/S Polypeptide variants
CN114806906B (zh) * 2022-05-31 2023-02-21 江南大学 一株产中性乳糖酶的乳酸克鲁维酵母
CN118931882A (zh) * 2023-05-11 2024-11-12 山东恒鲁生物科技有限公司 一种改良的成熟多肽

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5492813A (en) * 1993-11-01 1996-02-20 Boehringer Mannheim Corporation Muteins of β-galactosidase fragments having increased activity
AU1368300A (en) * 1999-07-09 2001-01-30 Universite De Liege Cold-active beta galactosidase, the process for its preparation and the use thereof
AU2001262068B2 (en) * 2000-05-26 2006-07-06 Arla Foods Amba A new enzyme isolated from a bifidobacterium
WO2004074468A1 (en) 2002-05-30 2004-09-02 Dsm Ip Assets B.V. Novel pectinases and uses thereof
EP3563688A1 (en) * 2005-11-28 2019-11-06 DSM IP Assets B.V. Enzyme preparations yielding a clean taste
US20110265221A1 (en) * 2007-07-10 2011-10-27 Monsanto Technology Llc Transgenic plants with enhanced agronomic traits
CN102250856B (zh) * 2011-06-23 2013-09-18 江南大学 一种耐热β-半乳糖苷酶突变体的构建
ES2397334B1 (es) * 2011-06-24 2014-06-06 Queizúar, S.L. Cepa de levadura kluyveromyces lactis y procedimiento de obtención de azúcares, etanol, beta-galactosidasa y biomasa.

Also Published As

Publication number Publication date
JP6632526B2 (ja) 2020-01-22
BR112016016342A2 (pt) 2017-10-03
CN105899659B (zh) 2025-06-17
EP3094723A1 (en) 2016-11-23
DK3094723T3 (en) 2018-06-06
BR112016016342B1 (pt) 2023-05-09
MX2016009149A (es) 2016-09-09
US9994837B2 (en) 2018-06-12
EP3094723B1 (en) 2018-02-28
US10612013B2 (en) 2020-04-07
AU2015206094A1 (en) 2016-06-09
JP2017507644A (ja) 2017-03-23
US20160333331A1 (en) 2016-11-17
CN105899659A (zh) 2016-08-24
AU2015206094B2 (en) 2020-09-10
US20180320156A1 (en) 2018-11-08
NZ720283A (en) 2022-03-25
MX375778B (es) 2025-03-07
EP3339433A1 (en) 2018-06-27
WO2015107050A1 (en) 2015-07-23

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