JP2017506877A5 - - Google Patents

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JP2017506877A5
JP2017506877A5 JP2016544526A JP2016544526A JP2017506877A5 JP 2017506877 A5 JP2017506877 A5 JP 2017506877A5 JP 2016544526 A JP2016544526 A JP 2016544526A JP 2016544526 A JP2016544526 A JP 2016544526A JP 2017506877 A5 JP2017506877 A5 JP 2017506877A5
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sample
rna
adapter molecule
molecule
nucleic acid
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JP2016544526A 2013-12-30 2014-12-30 核酸バーコードを用いた単一細胞と関連づけられた核酸の分析方法 Active JP6608368B2 (ja)

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US201361922012P 2013-12-30 2013-12-30
US61/922,012 2013-12-30
PCT/US2014/072898 WO2015103339A1 (en) 2013-12-30 2014-12-30 Analysis of nucleic acids associated with single cells using nucleic acid barcodes

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JP2017506877A JP2017506877A (ja) 2017-03-16
JP2017506877A5 true JP2017506877A5 (enExample) 2018-02-15
JP6608368B2 JP6608368B2 (ja) 2019-11-20

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US (7) US9580736B2 (enExample)
EP (2) EP4094834A1 (enExample)
JP (1) JP6608368B2 (enExample)
KR (2) KR20220119751A (enExample)
CN (2) CN114717291A (enExample)
AU (1) AU2014373757B2 (enExample)
CA (1) CA2935122C (enExample)
DK (1) DK3089822T3 (enExample)
ES (1) ES2912183T3 (enExample)
PL (1) PL3089822T3 (enExample)
PT (1) PT3089822T (enExample)
SG (2) SG11201605344YA (enExample)
WO (1) WO2015103339A1 (enExample)

Families Citing this family (190)

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Publication number Priority date Publication date Assignee Title
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
EP2854058A3 (en) 2010-05-18 2015-10-28 Natera, Inc. Methods for non-invasive pre-natal ploidy calling
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2670894B1 (en) 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
RU2671980C2 (ru) 2011-02-09 2018-11-08 Натера, Инк. Способы неинвазивного пренатального установления плоидности
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US11435358B2 (en) 2011-06-23 2022-09-06 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
ES2663234T3 (es) 2012-02-27 2018-04-11 Cellular Research, Inc Composiciones y kits para recuento molecular
WO2013134261A1 (en) 2012-03-05 2013-09-12 President And Fellows Of Harvard College Systems and methods for epigenetic sequencing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
CN104769127A (zh) 2012-08-14 2015-07-08 10X基因组学有限公司 微胶囊组合物及方法
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN108753766A (zh) 2013-02-08 2018-11-06 10X基因组学有限公司 多核苷酸条形码生成
AU2013382098B2 (en) 2013-03-13 2019-02-07 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US10119134B2 (en) * 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
EP3480321B8 (en) 2013-08-28 2021-03-10 Becton, Dickinson and Company Massively parallel single cell analysis
CN105745528A (zh) 2013-10-07 2016-07-06 赛卢拉研究公司 用于以数字方式对阵列上的特征进行计数的方法和系统
ES2890078T3 (es) 2013-12-20 2022-01-17 Illumina Inc Conservación de la información de conectividad genómica en muestras de ADN genómico fragmentado
CN114717291A (zh) * 2013-12-30 2022-07-08 阿特雷卡公司 使用核酸条形码分析与单细胞缔合的核酸
CN106413896B (zh) 2014-04-10 2019-07-05 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
DK3134536T3 (da) * 2014-04-21 2020-02-03 Harvard College Systemer og fremgangsmåder til at stregkodemarkere nukleinsyrer
CN106460070B (zh) 2014-04-21 2021-10-08 纳特拉公司 检测染色体片段中的突变和倍性
EP3137601B1 (en) * 2014-04-29 2020-04-08 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
US10975371B2 (en) * 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN118910216A (zh) * 2014-06-26 2024-11-08 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
US20150376700A1 (en) 2014-06-26 2015-12-31 10X Genomics, Inc. Analysis of nucleic acid sequences
ES2727656T3 (es) * 2014-09-15 2019-10-17 Abvitro Llc Secuenciación de alto rendimiento de banco de nucleótidos
EP3207134B1 (en) * 2014-10-17 2019-07-03 Illumina Cambridge Limited Contiguity preserving transposition
US11873480B2 (en) 2014-10-17 2024-01-16 Illumina Cambridge Limited Contiguity preserving transposition
CA2964472A1 (en) 2014-10-29 2016-05-06 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
US10697010B2 (en) 2015-02-19 2020-06-30 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
CN107532202A (zh) 2015-02-24 2018-01-02 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
US20160257993A1 (en) * 2015-02-27 2016-09-08 Cellular Research, Inc. Methods and compositions for labeling targets
ES2836802T3 (es) 2015-02-27 2021-06-28 Becton Dickinson Co Códigos de barras moleculares espacialmente direccionables
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
CA2983122A1 (en) 2015-04-17 2016-10-20 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
CN107580632B (zh) 2015-04-23 2021-12-28 贝克顿迪金森公司 用于全转录组扩增的方法和组合物
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
EP3341494B1 (en) * 2015-08-28 2024-08-07 Illumina, Inc. Nucleic acid sequence analysis from single cells
WO2017044574A1 (en) 2015-09-11 2017-03-16 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
KR20180085717A (ko) 2015-09-24 2018-07-27 에이비비트로, 엘엘씨 친화성-올리고뉴클레오타이드 콘쥬게이트 및 그것의 사용
WO2017079593A1 (en) * 2015-11-04 2017-05-11 Atreca, Inc. Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
US20170141793A1 (en) * 2015-11-13 2017-05-18 Microsoft Technology Licensing, Llc Error correction for nucleotide data stores
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
US11965891B2 (en) * 2015-12-30 2024-04-23 Bio-Rad Laboratories, Inc. Digital protein quantification
WO2017121832A1 (en) * 2016-01-14 2017-07-20 European Molecular Biology Laboratory Microfluidic analysis of ligand induced cell expression
ES3002715T3 (en) 2016-04-14 2025-03-07 Guardant Health Inc Methods for early detection of cancer
ES2913468T3 (es) 2016-04-15 2022-06-02 Natera Inc Métodos para la detección del cáncer de pulmón.
WO2017181161A1 (en) * 2016-04-15 2017-10-19 Predicine, Inc. Systems and methods for detecting genetic alterations
WO2017192387A1 (en) 2016-05-02 2017-11-09 Cellular Research, Inc. Accurate molecular barcoding
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US20170342463A1 (en) * 2016-05-25 2017-11-30 Bio-Rad Laboratories, Inc. Digital proximity assay
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US11397882B2 (en) 2016-05-26 2022-07-26 Becton, Dickinson And Company Molecular label counting adjustment methods
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
CN105950612B (zh) * 2016-07-08 2019-06-21 北京全式金生物技术有限公司 一种高效的dna接头连接方法
WO2018027048A1 (en) * 2016-08-05 2018-02-08 Bio-Rad Laboratories, Inc. Second strand direct
EP3497220A4 (en) * 2016-08-10 2020-04-01 Grail, Inc. METHODS OF PREPARING DOUBLE-INDEXING DNA LIBRARIES FOR BISULPHITE CONVERSION SEQUENCING
EP3516400B1 (en) 2016-09-26 2023-08-16 Becton, Dickinson and Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
CN109983126A (zh) * 2016-10-19 2019-07-05 10X基因组学有限公司 用于条形码化单个细胞或细胞群的核酸分子的方法和系统
ES2870639T3 (es) 2016-10-24 2021-10-27 Geneinfosec Inc Ocultación de información presente en los ácidos nucleicos
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
CN109952612B (zh) 2016-11-08 2023-12-01 贝克顿迪金森公司 用于表达谱分类的方法
EP4628595A3 (en) 2016-11-08 2025-11-05 Becton, Dickinson and Company Methods for cell label classification
US11479806B2 (en) 2016-11-10 2022-10-25 Takara Bio Usa, Inc. Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein
US10011870B2 (en) * 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3559262B1 (en) * 2016-12-22 2025-04-09 Illumina, Inc. Arrays with quality control tracers
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
ES2961580T3 (es) 2017-01-13 2024-03-12 Cellular Res Inc Revestimiento hidrófilo de canales de fluidos
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
EP3375889B1 (en) * 2017-03-17 2019-12-11 HiFiBiO SAS Single cell analysis
EP3885445B1 (en) * 2017-04-14 2023-08-23 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
CA3062287A1 (en) * 2017-04-21 2018-10-25 Mesa Biotech, Inc. Fluidic test cassette
WO2018195878A1 (zh) * 2017-04-27 2018-11-01 深圳华大基因股份有限公司 Dna标签及其应用
EP3620535A4 (en) 2017-05-02 2021-01-13 The University of Tokyo METHOD FOR THE INTEGRAL DETECTION OF NON-DESTRUCTIVE MEASUREMENT INFORMATION AND GENOMIC INFORMATION OF A CELL
AU2018262331A1 (en) * 2017-05-05 2019-11-21 Scipio Bioscience Methods for trapping and barcoding discrete biological units in hydrogel
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
CN110719959B (zh) * 2017-06-05 2021-08-06 贝克顿迪金森公司 针对单细胞的样品索引
CN107502607A (zh) * 2017-06-20 2017-12-22 浙江大学 一种大量组织、细胞样本mRNA的分子条形码标记、文库构建、测序的方法
WO2019060716A1 (en) 2017-09-25 2019-03-28 Freenome Holdings, Inc. SAMPLE EXTRACTION METHODS AND SYSTEMS
EP3700672B1 (en) * 2017-10-27 2022-12-28 10X Genomics, Inc. Methods for sample preparation and analysis
US20190153438A1 (en) 2017-11-15 2019-05-23 Viome, Inc. Methods and compositions for preparing polynucleotide libraries
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019104337A1 (en) 2017-11-27 2019-05-31 The Trustees Of Columbia University In The City Of New York Rna printing and sequencing devices, methods, and systems
WO2019113563A1 (en) * 2017-12-09 2019-06-13 Viome, Inc. Methods for nucleic acid library creation
US12084720B2 (en) 2017-12-14 2024-09-10 Natera, Inc. Assessing graft suitability for transplantation
CN111492068B (zh) * 2017-12-19 2025-03-21 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
KR102014470B1 (ko) * 2017-12-29 2019-08-26 한국과학기술원 핵산의 절단 및 중합연쇄반응 시스템 기반 등온 핵산증폭기술을 이용한 표적 rna 검출 방법
WO2019133874A1 (en) * 2017-12-31 2019-07-04 Berkeley Lights, Inc. General functional assay
WO2019147663A1 (en) * 2018-01-24 2019-08-01 Freenome Holdings, Inc. Methods and systems for abnormality detection in the patterns of nucleic acids
SG11202007686VA (en) * 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
EP3755812A1 (en) * 2018-02-22 2020-12-30 10X Genomics, Inc. Ligation mediated analysis of nucleic acids
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
EP3755799A4 (en) * 2018-02-23 2021-12-08 Yale University LYSIS BY FREEZE-DEFROST OF A SINGLE CELL
EP3762494B1 (en) * 2018-03-07 2022-11-23 Wisconsin Alumni Research Foundation High throughput nucleic acid profiling of single cells
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
JP7573443B2 (ja) 2018-04-14 2024-10-25 ナテラ, インコーポレイテッド 循環腫瘍dnaの個別化された検出を用いる癌検出およびモニタリングの方法
AU2019262048B2 (en) 2018-05-03 2025-09-04 Becton, Dickinson And Company High throughput multiomics sample analysis
WO2019213237A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
IL271454B2 (en) * 2018-05-17 2025-04-01 Illumina Inc Rapid single-cell genetic sequencing with low Aggregation bias
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CN112513269A (zh) 2018-07-12 2021-03-16 德克萨斯大学系统董事会 通过寡核苷酸进行分子邻域检测
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
EP3837545A1 (en) 2018-08-17 2021-06-23 F. Hoffmann-La Roche AG In vitro transcytosis assay
CN112912513A (zh) * 2018-08-28 2021-06-04 贝克顿迪金森公司 使用糖类结合试剂和膜透过性试剂进行样品多重化
WO2020072380A1 (en) * 2018-10-01 2020-04-09 Cellular Research, Inc. Determining 5' transcript sequences
CA3117476A1 (en) 2018-10-05 2020-04-09 Board Of Regents, The University Of Texas System Solid-phase n-terminal peptide capture and release
WO2020096015A1 (ja) 2018-11-07 2020-05-14 国立大学法人 東京大学 1種以上の被検物質と共存した細胞のゲノム関連情報を検出する方法
EP3877520B1 (en) 2018-11-08 2025-03-19 Becton, Dickson And Company Whole transcriptome analysis of single cells using random priming
WO2020118200A1 (en) * 2018-12-07 2020-06-11 Qiagen Sciences, Llc Methods for preparing cdna samples for rna sequencing, and cdna samples and uses thereof
US11459607B1 (en) * 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
CN111378557B (zh) 2018-12-26 2023-06-06 财团法人工业技术研究院 用于产生液珠的管状结构及液珠产生方法
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
US11661631B2 (en) 2019-01-23 2023-05-30 Becton, Dickinson And Company Oligonucleotides associated with antibodies
EP3924506A1 (en) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybrid targeted and whole transcriptome amplification
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
EP3959330A4 (en) * 2019-04-24 2023-07-05 Molecular Assemblies, Inc. HOMOPOLYMER ENCODED NUCLEIC ACID MEMORY
CN110241460A (zh) * 2019-05-31 2019-09-17 南方医科大学南方医院 一种甄别独立样品自身交叉反应的免疫组库方法
WO2020247263A1 (en) 2019-06-06 2020-12-10 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
CN114051534B (zh) 2019-07-22 2025-02-21 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
KR20220076453A (ko) * 2019-08-14 2022-06-08 아이리퍼트와, 인크. 올리고-dT 역전사된 RNA로부터 TCR 알파 및 베타 쇄 VDJ를 회수하는 프로브 포획 방법
US11919000B2 (en) 2019-10-10 2024-03-05 1859, Inc. Methods and systems for microfluidic screening
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
JP7107535B2 (ja) * 2020-01-10 2022-07-27 シンクサイト株式会社 新規細胞表現型スクリーニング方法
AU2021207581A1 (en) 2020-01-13 2022-08-11 Fluent Biosciences Inc. Methods and systems for single cell gene profiling
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
EP4090739A4 (en) 2020-01-13 2024-02-14 Fluent Biosciences Inc. Single cell sequencing
WO2021146187A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Emulsion based drug screening
CN115175999A (zh) 2020-01-22 2022-10-11 阿特雷卡公司 多个转录物的高通量连接
EP4093884A4 (en) * 2020-01-24 2024-05-15 Vanderbilt University Methods for identification of ligand-blocking antibodies and for determining antibody potency
US12188010B2 (en) 2020-01-29 2025-01-07 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
US12428685B2 (en) 2020-03-24 2025-09-30 Illumina, Inc. Viral detection using template emulsification
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
EP4158055B1 (en) 2020-06-02 2024-03-27 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12241059B2 (en) 2020-07-15 2025-03-04 Illumina, Inc. Tiered ligation oligos
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
WO2022133278A2 (en) * 2020-12-18 2022-06-23 Ionis Pharmaceuticals, Inc. Compounds and methods for modulating factor xii
US20240042442A1 (en) * 2020-12-22 2024-02-08 Hifibio Sas Microfluidic methods and systems
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
EP4305205A4 (en) * 2021-03-10 2025-08-06 Factorial Diagnostics Inc CELL BARCODE COMPOSITIONS AND RELATED METHODS
JP2024544571A (ja) * 2021-11-16 2024-12-03 パイオニア ハイ-ブレッド インターナショナル, インコーポレイテッド トウモロコシイベントdas-01131-3及びその検出方法
EP4353825A1 (en) * 2022-10-10 2024-04-17 bisy GmbH Modified promoter sequences
WO2025019326A2 (en) * 2023-07-14 2025-01-23 President And Fellows Of Harvard College In situ amplification of rna
WO2025085431A1 (en) * 2023-10-16 2025-04-24 Bio-Rad Laboratories, Inc. Enzymatic selection of nucleic acid

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5624711A (en) * 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
EP1510251B1 (en) 2001-02-23 2006-09-06 Japan Science and Technology Agency Process and apparatus for producing microcapsules
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
US7976779B2 (en) * 2002-06-26 2011-07-12 California Institute Of Technology Integrated LC-ESI on a chip
JP2007525963A (ja) * 2003-06-20 2007-09-13 イルミナ インコーポレイテッド 全ゲノム増幅および遺伝型決定のための方法および組成物
TWI333977B (en) 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
EP1757357B1 (en) 2004-03-23 2013-04-24 Japan Science and Technology Agency Method and device for producing micro-droplets
JOP20080381B1 (ar) 2007-08-23 2023-03-28 Amgen Inc بروتينات مرتبطة بمولدات مضادات تتفاعل مع بروبروتين كونفيرتاز سيتيليزين ككسين من النوع 9 (pcsk9)
ES2391906T3 (es) 2007-09-12 2012-11-30 Institut Pasteur Polinucleótido apto para un ensayo indicador basado en una sola célula para controlar los patrones de expresión génica con una resolución espacio-temporal elevada
US20100179075A1 (en) * 2008-07-30 2010-07-15 Life Technologies Corporation Particles for use in supported nucleic acid ligation and detection sequencing
US9625454B2 (en) 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
EP2420579A1 (en) * 2010-08-17 2012-02-22 QIAGEN GmbH Helicase dependent isothermal amplification using nicking enzymes
US8883421B2 (en) * 2010-09-10 2014-11-11 New England Biolabs, Inc. Method for reducing adapter-dimer formation
ES2966040T3 (es) * 2010-10-08 2024-04-18 Harvard College Alto rendimiento de células individuales con código de barra
DK2625295T3 (da) 2010-10-08 2019-06-11 Harvard College High-throughput-immunsekvensering
DK2652155T3 (en) * 2010-12-16 2017-02-13 Gigagen Inc Methods for Massive Parallel Analysis of Nucleic Acids in Single Cells
EP3736281A1 (en) * 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
SI3418380T1 (sl) 2011-04-28 2022-10-28 The Board Of Trustees Of The Leland Stanford Junior University Identifikacija polinukleotidov, povezanih z vzorcem
EP2705156B1 (en) * 2011-05-05 2015-08-26 Institut National de la Santé et de la Recherche Médicale (INSERM) Linear dna amplification
US20150125865A1 (en) 2011-12-23 2015-05-07 Gigagen, Inc. Methods And Apparatuses For Droplet Mixing
US9176031B2 (en) * 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
WO2013134261A1 (en) 2012-03-05 2013-09-12 President And Fellows Of Harvard College Systems and methods for epigenetic sequencing
NO2694769T3 (enExample) * 2012-03-06 2018-03-03
CN104471077B (zh) * 2012-05-21 2017-05-24 富鲁达公司 颗粒群的单颗粒分析
CN104619862B (zh) 2012-06-15 2017-10-31 得克萨斯系统大学董事会 单细胞的多转录物的高通量测序
WO2014108850A2 (en) * 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
CN115558661A (zh) * 2013-08-23 2023-01-03 路德维格癌症研究院 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物
GB201317301D0 (en) * 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
CN114717291A (zh) 2013-12-30 2022-07-08 阿特雷卡公司 使用核酸条形码分析与单细胞缔合的核酸
US20150298091A1 (en) * 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids

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