JP2017106933A5 - - Google Patents

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JP2017106933A5
JP2017106933A5 JP2017049533A JP2017049533A JP2017106933A5 JP 2017106933 A5 JP2017106933 A5 JP 2017106933A5 JP 2017049533 A JP2017049533 A JP 2017049533A JP 2017049533 A JP2017049533 A JP 2017049533A JP 2017106933 A5 JP2017106933 A5 JP 2017106933A5
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nucleic acid
acid molecule
molecule
nanopore
progression
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JP2017049533A 2012-02-16 2017-03-15 標的分子を検出する方法 Active JP6431108B2 (ja)

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US201261599871P 2012-02-16 2012-02-16
US61/599,871 2012-02-16
US201261600398P 2012-02-17 2012-02-17
US61/600,398 2012-02-17

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JP2017106933A JP2017106933A (ja) 2017-06-15
JP2017106933A5 true JP2017106933A5 (https=) 2018-04-12
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JP2014557852A Active JP6178805B2 (ja) 2012-02-16 2013-02-15 ナノ細孔センサーとともに使用するための二重層を作製するための方法
JP2017049533A Active JP6431108B2 (ja) 2012-02-16 2017-03-15 標的分子を検出する方法
JP2018034689A Active JP6617167B2 (ja) 2012-02-16 2018-02-28 ナノ細孔センサーとともに使用するための二重層を作製するための方法
JP2019094447A Withdrawn JP2019164155A (ja) 2012-02-16 2019-05-20 ナノ細孔センサーとともに使用するための二重層を作製するための方法

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JP2019094447A Withdrawn JP2019164155A (ja) 2012-02-16 2019-05-20 ナノ細孔センサーとともに使用するための二重層を作製するための方法

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US (5) US9850534B2 (https=)
EP (2) EP3540077B1 (https=)
JP (4) JP6178805B2 (https=)
CN (2) CN104254619B (https=)
CA (1) CA2864125C (https=)
ES (1) ES2732256T3 (https=)
WO (1) WO2013123450A1 (https=)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012083249A2 (en) 2010-12-17 2012-06-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
CN107828877A (zh) * 2012-01-20 2018-03-23 吉尼亚科技公司 基于纳米孔的分子检测与测序
CN104254619B (zh) 2012-02-16 2018-08-24 吉尼亚科技公司 产生用于纳米孔传感器的双层的方法
WO2013154999A2 (en) 2012-04-09 2013-10-17 The Trustees Of Columbia University In The City Of New York Method of preparation of nanopore and uses thereof
WO2013188841A1 (en) * 2012-06-15 2013-12-19 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
EP3674412A1 (en) * 2012-06-20 2020-07-01 The Trustees of Columbia University in the City of New York Nucleic acid sequencing by nanopore detection of tag molecules
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US10732183B2 (en) 2013-03-15 2020-08-04 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
KR102245192B1 (ko) 2013-05-06 2021-04-29 온테라 인크. 나노포어를 이용한 표적 검출
ES2704902T3 (es) 2013-05-06 2019-03-20 Two Pore Guys Inc Un método de detección de objetivos biológicos usando un nanoporo y un agente de unión a proteínas de fusión
WO2014190299A2 (en) * 2013-05-23 2014-11-27 Arizona Board Of Regents Acting For And On Behalf Of Arizona State University Chemistry, systems and methods of translocation of a polymer through a nanopore
US9322062B2 (en) * 2013-10-23 2016-04-26 Genia Technologies, Inc. Process for biosensor well formation
JP6461943B2 (ja) * 2013-10-23 2019-01-30 ジェニア・テクノロジーズ・インコーポレイテッド ナノポアを備えた高速分子検知
MA39774A (fr) 2014-03-24 2021-05-12 Roche Sequencing Solutions Inc Procédés chimiques pour produire des nucléotides étiquetés
ES2846746T3 (es) * 2014-06-03 2021-07-29 Illumina Inc Composiciones, sistemas y métodos para detectar acontecimientos usando fijaciones ancladas o adyacentes a nanoporos
CA3163156C (en) * 2014-07-31 2025-07-08 Illumina, Inc. HYBRID NANOPORE SENSORS
WO2016069806A2 (en) 2014-10-31 2016-05-06 Genia Technologies, Inc. Alpha-hemolysin variants with altered characteristics
US10036739B2 (en) 2015-01-27 2018-07-31 Genia Technologies, Inc. Adjustable bilayer capacitance structure for biomedical devices
CN104651500B (zh) * 2015-01-30 2017-06-30 华东理工大学 气单胞菌溶素纳米孔通道的制备方法及其应用
WO2016124543A1 (en) 2015-02-02 2016-08-11 F. Hoffmann-La Roche Ag Polymerase variants
US9791432B2 (en) * 2015-03-20 2017-10-17 Genia Technologies, Inc. Serpentine flow channels for flowing fluids over chip sensors
TWI571626B (zh) * 2015-07-15 2017-02-21 力晶科技股份有限公司 具有奈米腔的集成生物感測器及其製作方法
US10174371B2 (en) 2015-08-05 2019-01-08 Genia Technologies, Inc. Use of titanium nitride as an electrode in non-faradaic electrochemical cell
US10809243B2 (en) 2015-08-31 2020-10-20 Roche Sequencing Solutions, Inc. Small aperture large electrode cell
WO2017050723A1 (en) 2015-09-22 2017-03-30 Genia Technologies, Inc. Pol7 polymerase variants
US10935512B2 (en) * 2015-09-24 2021-03-02 Roche Sequencing Solutions, Inc. Encoding state change of nanopore to reduce data size
US10102338B2 (en) 2015-09-24 2018-10-16 Genia Technologies, Inc. Adaptive compression and modification of nanopore measurement data
JP6889701B2 (ja) 2015-09-24 2021-06-18 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft アルファ溶血素バリアント
JP2018533010A (ja) * 2015-10-21 2018-11-08 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft ナノポアベースのdna配列決定のための脂質二重層形成を支援するための撥水性層としてのフルオロポリマーの使用
CN105368938B (zh) * 2015-11-06 2018-09-21 中国科学院重庆绿色智能技术研究院 一种基于电击穿在氮化硅薄膜上精确制备纳米孔的方法
US10718737B2 (en) 2016-01-21 2020-07-21 Roche Sequencing Solutions, Inc. Molded flow channel
CN108699539B (zh) 2016-02-29 2022-11-18 吉尼亚科技公司 外切核酸酶缺陷的聚合酶
US10465240B2 (en) * 2016-03-30 2019-11-05 Roche Sequencing Solutions, Inc. Electrical enhancement of bilayer formation
WO2017167811A1 (en) 2016-03-31 2017-10-05 Genia Technologies, Inc. Nanopore protein conjugates and uses thereof
JP7027334B2 (ja) 2016-04-21 2022-03-01 エフ.ホフマン-ラ ロシュ アーゲー アルファ溶血素バリアントおよびその使用
EP3475696B1 (en) 2016-06-27 2020-04-08 H. Hoffnabb-La Roche Ag Osmotic imbalance methods for bilayer formation
CN109415419B (zh) 2016-06-30 2022-07-29 豪夫迈·罗氏有限公司 长寿α-溶血素纳米孔
GB201612458D0 (en) * 2016-07-14 2016-08-31 Howorka Stefan And Pugh Genevieve Membrane spanning DNA nanopores for molecular transport
CN106443008A (zh) * 2016-08-31 2017-02-22 中国科学院重庆绿色智能技术研究院 一种基于固态纳米孔的hiv‑1蛋白酶检测方法
JP7157048B2 (ja) 2016-09-22 2022-10-19 エフ.ホフマン-ラ ロシュ アーゲー Pol6ポリメラーゼバリアント
WO2018136487A1 (en) 2017-01-20 2018-07-26 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
WO2018152050A1 (en) * 2017-02-14 2018-08-23 Axbio Inc. Apparatus and methods for continuous diagnostics of macromolecules
EP3655423B1 (en) * 2017-07-17 2023-12-20 President and Fellows of Harvard College Nanopore-matched protein shuttle for molecular characterization
EP3697932A1 (en) * 2017-10-19 2020-08-26 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
JP7012841B2 (ja) * 2017-10-23 2022-01-28 エフ.ホフマン-ラ ロシュ アーゲー 浸透性の不均衡を利用した膜内のタンパク質ナノポアの除去および再挿入
CN109709185B (zh) * 2017-10-25 2024-09-24 深圳宣泽生物医药有限公司 一种修饰生物探针的纳米孔检测装置及制作方法
KR20200119291A (ko) 2018-02-15 2020-10-19 에프. 호프만-라 로슈 아게 분석물의 검출 및 분석을 위한 나노포어 단백질 접합체
WO2019166458A1 (en) 2018-02-28 2019-09-06 F. Hoffmann-La Roche Ag Alpha-hemolysin variants and uses thereof
EP3775893A1 (en) * 2018-04-13 2021-02-17 F. Hoffmann-La Roche AG Methods and compositions for detection and analysis of analytes
CN112204154B (zh) 2018-05-28 2024-06-25 豪夫迈·罗氏有限公司 Dna-孔隙-聚合酶复合物的酶促富集
EP3815092A2 (en) 2018-06-29 2021-05-05 F. Hoffmann-La Roche AG Detection of microsatellite instability
CN113260449B (zh) * 2018-12-11 2023-09-29 豪夫迈·罗氏有限公司 用于膜中自限性蛋白质孔插入的系统和方法
JP7162283B2 (ja) * 2019-05-27 2022-10-28 国立大学法人山形大学 脂質二分子膜チャネル評価チップ及びその製造方法並びに評価装置
US20220259667A1 (en) 2019-07-22 2022-08-18 Roche Sequencing Solutions, Inc. Systems and methods for cell of origin determination from variant calling data
EP4003382A4 (en) * 2019-07-31 2023-10-11 Axbio Inc. SYSTEMS AND METHODS FOR EVALUATION OF A TARGET MOLECULE
WO2021053008A1 (en) 2019-09-20 2021-03-25 F. Hoffmann-La Roche Ag Immune repertoire profiling by primer extension target enrichment
CN114630891B (zh) * 2019-10-30 2025-06-10 豪夫迈·罗氏有限公司 用于生物纳米孔的组装的方法和组合物
WO2021099521A1 (en) 2019-11-21 2021-05-27 F. Hoffmann-La Roche Ag Systems and methods for contamination detection in next generation sequencing samples
CN120442767A (zh) 2020-05-28 2025-08-08 豪夫迈·罗氏有限公司 识别高错误单分子读段中的短基序的序列比对系统和方法
JP7831788B2 (ja) * 2020-09-08 2026-03-17 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 一体化された細孔ベースの検出によるラテラルフロー核酸アッセイ
CN114249294B (zh) * 2020-09-22 2025-07-04 上海新微技术研发中心有限公司 一种多层mems结构及其制作方法与应用
US12378596B2 (en) 2020-12-03 2025-08-05 Roche Sequencing Solutions, Inc. Whole transcriptome analysis in single cells
JP7646850B2 (ja) 2021-02-09 2025-03-17 エフ. ホフマン-ラ ロシュ アーゲー 核酸中のメチル化の塩基レベル検出のための方法
US20240301394A1 (en) 2021-03-03 2024-09-12 Roche Sequencing Solutions, Inc. Devices and methods for electrophoretic extraction of nucleic acids from biological samples
CN115125098B (zh) * 2021-03-29 2025-05-27 上海近观科技有限责任公司 基于分子封层及加热密封结构的纳米孔检测装置及方法
WO2022248237A1 (en) 2021-05-24 2022-12-01 F. Hoffmann-La Roche Ag Enhancer oligonucleotides for nucleic acid hybridization
WO2023107899A2 (en) 2021-12-07 2023-06-15 Caribou Biosciences, Inc. A method of capturing crispr endonuclease cleavage products
CN114410459A (zh) * 2022-01-11 2022-04-29 深圳清华大学研究院 基因测序装置和测序方法
US20250361549A1 (en) 2022-06-14 2025-11-27 Roche Sequencing Solutions, Inc. Detection of epigenetic cytosine modification
WO2024038069A1 (en) 2022-08-18 2024-02-22 F. Hoffmann-La Roche Ag Detection of epigenetic modifications
TWI862997B (zh) * 2022-09-30 2024-11-21 國立中興大學 微流體感測裝置、微流體感測系統及微流體感測方法
CN115651821B (zh) * 2022-12-07 2023-04-07 北京齐碳科技有限公司 一种分子检测单元、芯片以及制备方法
CN116381250A (zh) * 2023-03-03 2023-07-04 南京大学 基于纳米孔道蛋白的高通量检测装置
AU2024246785A1 (en) 2023-03-30 2025-09-11 F. Hoffmann-La Roche Ag Modulation of target molecule-lipid bilayer interactions
WO2025024676A1 (en) 2023-07-26 2025-01-30 Caribou Biosciences, Inc. In vitro validation methods for cd19-targeting cell therapies
WO2025106652A1 (en) * 2023-11-17 2025-05-22 Illumina, Inc. Fluidic devices, nanopore instruments, and methods
WO2025193609A1 (en) 2024-03-11 2025-09-18 Caribou Biosciences, Inc. Methods and compositions for cost-effective assessment of nucleic acid transcripts and isoforms in cells
WO2025221998A1 (en) 2024-04-17 2025-10-23 Roche Sequencing Solutions, Inc. Systems and methods for variant calling
WO2025221988A1 (en) 2024-04-17 2025-10-23 Roche Sequencing Solutions, Inc. Systems and methods for somatic small variant calling
WO2025231432A1 (en) 2024-05-03 2025-11-06 Caribou Biosciences, Inc. In vivo gene editing with crispr systems
WO2026039623A1 (en) 2024-08-14 2026-02-19 Roche Sequencing Solutions, Inc. Systems and methods for germline snv and indel variant calling
WO2026050617A1 (en) 2024-08-30 2026-03-05 Roche Sequencing Solutions, Inc. Systems and methods for clustering sequencing reads to identify pcr duplicates
WO2026055639A1 (en) 2024-09-06 2026-03-12 Cepheid Sample in sequencing-ready library out automated library preparation solution

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993021528A1 (en) * 1992-04-22 1993-10-28 Ecole Polytechnique Federale De Lausanne (Epfl) Lipid membrane sensors
US6413792B1 (en) * 2000-04-24 2002-07-02 Eagle Research Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US6952651B2 (en) * 2002-06-17 2005-10-04 Intel Corporation Methods and apparatus for nucleic acid sequencing by signal stretching and data integration
WO2004085609A2 (en) * 2003-02-28 2004-10-07 Brown University Nanopores, methods for using same, methods for making same and methods for characterizing biomolecules using same
US20050023156A1 (en) * 2003-07-30 2005-02-03 Ramsey J. Michael Nanostructured material transport devices and their fabrication by application of molecular coatings to nanoscale channels
US20050136408A1 (en) * 2003-12-19 2005-06-23 May Tom-Moy Methods and systems for characterizing a polymer
JP4953044B2 (ja) * 2005-05-09 2012-06-13 財団法人生産技術研究奨励会 脂質二重膜の形成方法およびその装置
WO2006128138A2 (en) * 2005-05-26 2006-11-30 Ensemble Discovery Corportion Biodetection by nucleic acid-templated chemistry
WO2007047498A2 (en) * 2005-10-14 2007-04-26 The Regents Of The University Of California Formation and encapsulation of molecular bilayer and monolayer membranes
US7777505B2 (en) * 2006-05-05 2010-08-17 University Of Utah Research Foundation Nanopore platforms for ion channel recordings and single molecule detection and analysis
US20110121840A1 (en) 2007-02-20 2011-05-26 Gurdial Singh Sanghera Lipid Bilayer Sensor System
EP2156179B1 (en) * 2007-04-04 2021-08-18 The Regents of The University of California Methods for using a nanopore
GB0713402D0 (en) 2007-07-11 2007-08-22 Cardiff & Vale Nhs Trust A method of diagnosing a condition using a neural network
BRPI0813718A2 (pt) * 2007-07-13 2014-12-30 Univ Leland Stanford Junior Método e aparelho que utilizam um campo elétrico para ensaios biológicos aperfeiçoados
JP5441142B2 (ja) 2007-11-26 2014-03-12 国立大学法人 東京大学 マイクロ流体による平面脂質二重膜アレイ及びその平面脂質二重膜を用いた分析方法
DE112008003078B4 (de) * 2007-11-30 2018-11-29 Electronic Bio Sciences, Llc Verfahren und Vorrichtung zur einseitigen Doppelschichtbildung
GB0724736D0 (en) * 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8628649B2 (en) * 2008-03-18 2014-01-14 Arizona Board Of Regents Acting For And On Behalf Of Arizona State University Nanopore and carbon nanotube based DNA sequencer and a serial recognition sequencer
WO2010082860A1 (en) * 2009-01-19 2010-07-22 Instituto De Biologia Experimental E Tecnologia (Ibet) Method and device for nanopore based single-molecule protein/ protein interaction detection
JP5372570B2 (ja) * 2009-03-30 2013-12-18 株式会社日立ハイテクノロジーズ ナノポアを用いたバイオポリマー決定方法、システム、及びキット
EP2483680A4 (en) * 2009-09-30 2014-01-01 Quantapore Inc ULTRASOUND SEQUENCING OF BIOLOGICAL POLYMERS WITH THE HELP OF A MARKED NANOPORE
US9605307B2 (en) * 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
WO2011097028A1 (en) * 2010-02-08 2011-08-11 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
WO2012009578A2 (en) * 2010-07-14 2012-01-19 The Curators Of The University Of Missouri Nanopore-facilitated single molecule detection of nucleic acids
GB2500360B (en) 2010-12-22 2019-10-23 Genia Tech Inc Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps
US8968539B2 (en) * 2011-03-08 2015-03-03 Electronic Biosciences, Inc. Methods for voltage-induced protein incorporation into planar lipid bilayers
JP6298404B2 (ja) * 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
CN104254619B (zh) 2012-02-16 2018-08-24 吉尼亚科技公司 产生用于纳米孔传感器的双层的方法
EP3532843B1 (en) * 2016-10-26 2020-11-18 F. Hoffmann-La Roche AG Multi-chip packaging of integrated circuits and flow cells for nanopore sequencing

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