JP2018075021A5 - - Google Patents
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- Publication number
- JP2018075021A5 JP2018075021A5 JP2017236383A JP2017236383A JP2018075021A5 JP 2018075021 A5 JP2018075021 A5 JP 2018075021A5 JP 2017236383 A JP2017236383 A JP 2017236383A JP 2017236383 A JP2017236383 A JP 2017236383A JP 2018075021 A5 JP2018075021 A5 JP 2018075021A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- nanopore
- translocase
- electrical signal
- translocated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261599754P | 2012-02-16 | 2012-02-16 | |
| US61/599,754 | 2012-02-16 | ||
| US201261713163P | 2012-10-12 | 2012-10-12 | |
| US61/713,163 | 2012-10-12 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014557827A Division JP6312607B2 (ja) | 2012-02-16 | 2013-02-15 | 酵素仲介タンパク質トランスロケーションのためのナノポアセンサー |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018075021A JP2018075021A (ja) | 2018-05-17 |
| JP2018075021A5 true JP2018075021A5 (https=) | 2018-06-28 |
| JP6594944B2 JP6594944B2 (ja) | 2019-10-23 |
Family
ID=48984893
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014557827A Active JP6312607B2 (ja) | 2012-02-16 | 2013-02-15 | 酵素仲介タンパク質トランスロケーションのためのナノポアセンサー |
| JP2017236383A Active JP6594944B2 (ja) | 2012-02-16 | 2017-12-08 | 酵素仲介タンパク質トランスロケーションのためのナノポアセンサー |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014557827A Active JP6312607B2 (ja) | 2012-02-16 | 2013-02-15 | 酵素仲介タンパク質トランスロケーションのためのナノポアセンサー |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US11339365B2 (https=) |
| EP (1) | EP2814939B1 (https=) |
| JP (2) | JP6312607B2 (https=) |
| KR (1) | KR102066758B1 (https=) |
| CN (1) | CN104619854B (https=) |
| AU (1) | AU2013221356B2 (https=) |
| BR (1) | BR112014020255B1 (https=) |
| CA (1) | CA2864824C (https=) |
| DK (1) | DK2814939T3 (https=) |
| WO (1) | WO2013123379A2 (https=) |
Families Citing this family (70)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| CN103842519B (zh) | 2011-04-04 | 2018-02-06 | 哈佛大学校长及研究员协会 | 通过局部电位测量进行的纳米孔感测 |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| BR112014020211A2 (pt) | 2012-02-16 | 2017-07-04 | Oxford Nanopore Tech Ltd | métodos para analisar uma série ordenada por tempo de medições de um polímero, para estimar a presença, ausência ou quantidade de um polímero alvo, e para determinar uma alteração em um polímero, programa de computador, e, dispositivos de análise e de diagnóstico |
| EP2906720A4 (en) | 2012-10-10 | 2016-06-01 | Univ Arizona | MOLECULAR BASED SYSTEMS AND DEVICES AND METHOD FOR PRODUCING THEM |
| GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
| EP2965073B1 (en) | 2013-03-05 | 2018-10-31 | Arizona Board Of Regents Acting For And On Behalf Of State Arizona University | Translocation of a polymer through a nanopore |
| GB201316849D0 (en) | 2013-09-23 | 2013-11-06 | Isis Innovation | Method |
| WO2015127387A1 (en) * | 2014-02-21 | 2015-08-27 | Northeastern University | Fluorescence-based analysis of biopolymers using nanopores |
| WO2015131073A1 (en) | 2014-02-27 | 2015-09-03 | Arizona Board Of Regents Acting For And On Behalf Of Arizona State University | Triazole-based reader molecules and methods for synthesizing and use thereof |
| US10519499B2 (en) * | 2014-07-31 | 2019-12-31 | Illumina, Inc. | Hybrid nanopore sensors |
| EP3971300B1 (en) | 2014-10-16 | 2024-06-05 | Oxford Nanopore Technologies plc | Sorting of polymers |
| US20160194698A1 (en) * | 2014-12-16 | 2016-07-07 | Arizona Board Of Regents On Behalf Of Arizona State University | Systems, apparatuses and methods for reading polymer sequence |
| US10794895B2 (en) | 2015-02-05 | 2020-10-06 | President And Fellows Of Harvard College | Nanopore sensor including fluidic passage |
| GB201508669D0 (en) | 2015-05-20 | 2015-07-01 | Oxford Nanopore Tech Ltd | Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown |
| US10126262B2 (en) * | 2015-09-24 | 2018-11-13 | Genia Technologies, Inc. | Differential output of analog memories storing nanopore measurement samples |
| GB201611770D0 (en) | 2016-07-06 | 2016-08-17 | Oxford Nanopore Tech | Microfluidic device |
| CN106443008A (zh) * | 2016-08-31 | 2017-02-22 | 中国科学院重庆绿色智能技术研究院 | 一种基于固态纳米孔的hiv‑1蛋白酶检测方法 |
| GB201619930D0 (en) | 2016-11-24 | 2017-01-11 | Oxford Nanopore Tech | Apparatus and methods for controlling insertion of a membrane channel into a membrane |
| WO2019006214A1 (en) * | 2017-06-29 | 2019-01-03 | President And Fellows Of Harvard College | DETERMINISTIC PROGRESSION OF POLYMERS THROUGH A NANOPORE |
| CN110914291B (zh) * | 2017-07-17 | 2024-10-29 | 哈佛大学校董委员会 | 用于分子表征的纳米孔匹配的蛋白质穿梭物 |
| JP7012841B2 (ja) * | 2017-10-23 | 2022-01-28 | エフ．ホフマン－ラ ロシュ アーゲー | 浸透性の不均衡を利用した膜内のタンパク質ナノポアの除去および再挿入 |
| GB2568895B (en) | 2017-11-29 | 2021-10-27 | Oxford Nanopore Tech Ltd | Microfluidic device |
| CN108226249B (zh) * | 2018-01-09 | 2021-02-26 | 深圳市梅丽纳米孔科技有限公司 | 一次性纳米孔生物传感器及其制作方法 |
| EP3768159B1 (en) * | 2018-03-20 | 2025-01-01 | Graphwear Technologies Inc. | Replaceable sensor systems |
| CN121472373A (zh) | 2018-05-22 | 2026-02-06 | 安序源有限公司 | 用于核酸测序的方法、系统和组合物 |
| GB2574048B (en) | 2018-05-24 | 2021-06-16 | Oxford Nanopore Tech Ltd | Nanopore sensor component with electrostatic discharge protection |
| MY204234A (en) | 2018-08-27 | 2024-08-16 | Regeneron Pharma | Use of raman spectroscopy in downstream purification |
| WO2020055246A1 (en) | 2018-09-11 | 2020-03-19 | Rijksuniversiteit Groningen | Biological nanopores having tunable pore diameters and uses thereof as analytical tools |
| WO2020061385A1 (en) * | 2018-09-19 | 2020-03-26 | Conagen Inc. | Controllable protein degradation via engineering degradation tag variants in corynebacterium host cells |
| US20210340192A1 (en) * | 2018-10-05 | 2021-11-04 | University Of Washington | Reporter constructs for nanopore-based detection of biological activity |
| EP3894056B1 (en) * | 2018-12-11 | 2025-01-29 | F. Hoffmann-La Roche AG | Systems and methods for self-limiting protein pore insertion in a membrane |
| US11789006B2 (en) | 2019-03-12 | 2023-10-17 | Oxford Nanopore Technologies Plc | Nanopore sensing device, components and method of operation |
| NL2023062B1 (en) * | 2019-05-03 | 2020-11-30 | Univ Delft Tech | Protein current trace signal acquisition using a nanopore |
| GB201907243D0 (en) * | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Sensing interactions between molecular entities and nanapores |
| CN112147185B (zh) * | 2019-06-29 | 2022-07-01 | 清华大学 | 一种控制多肽穿过纳米孔速度的方法及其应用 |
| CN110554079B (zh) * | 2019-09-26 | 2024-10-22 | 中国科学院重庆绿色智能技术研究院 | 一种基于纳米通道的抗体单分子检测系统及方法 |
| GB201915480D0 (en) | 2019-10-25 | 2019-12-11 | Oxford Nanopore Tech Ltd | Improved nanopore sensing device, components and method of manufacture |
| CN115279879A (zh) * | 2019-11-15 | 2022-11-01 | 机器生物公司 | 生成生物分子的方法和系统 |
| EP4061965A1 (en) * | 2019-11-19 | 2022-09-28 | Rijksuniversiteit Groningen | Artificial nanopores and uses and methods relating thereto |
| KR20220110213A (ko) * | 2019-12-02 | 2022-08-05 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 나노포어를 사용하여 표적 폴리펩티드를 특징분석하는 방법 |
| NL2024579B1 (en) | 2019-12-24 | 2021-09-06 | Univ Delft Tech | Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes |
| CN115398008B (zh) * | 2020-04-14 | 2025-05-09 | 成都今是科技有限公司 | 纳米孔制备和检测方法及其检测装置 |
| GB202016874D0 (en) | 2020-10-23 | 2020-12-09 | Oxford Nanopore Tech Ltd | Nanopore support structure and manufacture thereof |
| WO2022013551A1 (en) | 2020-07-17 | 2022-01-20 | Oxford Nanopore Technologies Limited | Nanopore sensing device |
| EP4185865B1 (en) | 2020-07-22 | 2026-04-29 | Oxford Nanopore Technologies PLC | Solid state nanopore formation |
| KR102309424B1 (ko) * | 2020-09-28 | 2021-10-07 | 한국생명공학연구원 | 나노포어를 이용한 리보스위치-표적 물질의 스크리닝 방법 |
| US11994508B2 (en) * | 2020-12-23 | 2024-05-28 | Northeastern University | Method and system for linearization and translocation of single protein molecules through nanopores |
| US11994484B2 (en) * | 2021-03-25 | 2024-05-28 | The Regents Of The University Of California | Apparatus and method for single cell discrimination |
| GB202112235D0 (en) | 2021-08-26 | 2021-10-13 | Oxford Nanopore Tech Ltd | Nanopore |
| WO2023055752A2 (en) | 2021-09-28 | 2023-04-06 | Axbio Inc. | Methods for processing a nucleic acid sample and compositions thereof |
| GB202118908D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| EP4565882A1 (en) * | 2022-08-02 | 2025-06-11 | Glyphic Biotechnologies, Inc. | Protein sequencing via coupling of polymerizable molecules |
| IL320502A (en) | 2022-10-28 | 2025-06-01 | Univ Groningen | Nanopore-based protein efflux |
| GB202216162D0 (en) | 2022-10-31 | 2022-12-14 | Oxford Nanopore Tech Plc | Method |
| GB202216905D0 (en) | 2022-11-11 | 2022-12-28 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| CN120265979A (zh) * | 2022-12-29 | 2025-07-04 | 深圳华大生命科学研究院 | 生物纳米孔传感器、其制备方法及应用 |
| EP4662489A1 (en) | 2023-02-07 | 2025-12-17 | Oxford University Innovation Limited | Method of characterising a peptide, polypeptide or protein using a nanopore |
| CN120917151A (zh) * | 2023-04-28 | 2025-11-07 | 清华大学 | 一种被修饰的多肽、多肽复合物、多肽混合物及控制多肽移位速度或表征多肽的系统和方法 |
| AU2024283017A1 (en) * | 2023-06-01 | 2025-12-11 | Oxford Nanopore Technologies Plc | Method and kit for rereading single protein molecules using a nanopore and an unfoldase |
| GB202313202D0 (en) * | 2023-08-30 | 2023-10-11 | Portal Biotech Ltd | Systems and methods for nanopore-based analyte detection |
| CN119667132A (zh) * | 2023-09-20 | 2025-03-21 | 西安电子科技大学 | 一种半合成纳米孔及其制备方法和蛋白质测序方法 |
| GB202317028D0 (en) | 2023-11-06 | 2023-12-20 | Oxford Nanopore Tech Ltd | Method |
| WO2025120010A1 (en) * | 2023-12-04 | 2025-06-12 | Adolphe Merkle Institute, University Of Fribourg | Ply nanopores |
| WO2025120150A1 (en) | 2023-12-08 | 2025-06-12 | Oxford Nanopore Technologies Plc | Methods and apparatus for forming apertures, methods and apparatus for unblocking apertures, methods of sensing molecular entities in apertures, and measurement systems |
| WO2026052817A1 (en) | 2024-09-06 | 2026-03-12 | Oxford Nanopore Technologies Plc | Method |
| WO2026052811A2 (en) | 2024-09-06 | 2026-03-12 | Oxford Nanopore Technologies Plc | Method |
| GB2644084A (en) | 2024-09-16 | 2026-03-18 | Oxford Nanopore Tech Plc | Augmented consensus and variant calling |
| GB202413747D0 (en) | 2024-09-18 | 2024-10-30 | Oxford Nanopore Tech Plc | Preprocessing nanopore signals |
| CN119470870B (zh) * | 2024-11-19 | 2025-10-17 | 广东工业大学 | 一种基于组合式纳米孔结构的蛋白质检测方法 |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| SE513280C2 (sv) | 1998-11-05 | 2000-08-14 | Forbo Int Sa | Beläggningsmaterial för golv och förfarande för dess framställning |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| JP2003502166A (ja) | 1999-06-22 | 2003-01-21 | プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ | 固体状態の次元的特徴の制御 |
| US6464842B1 (en) | 1999-06-22 | 2002-10-15 | President And Fellows Of Harvard College | Control of solid state dimensional features |
| US6428959B1 (en) | 1999-09-07 | 2002-08-06 | The Regents Of The University Of California | Methods of determining the presence of double stranded nucleic acids in a sample |
| AU2002239284A1 (en) | 2000-11-27 | 2002-06-03 | The Regents Of The University Of California | Methods and devices for characterizing duplex nucleic acid molecules |
| US20030104428A1 (en) | 2001-06-21 | 2003-06-05 | President And Fellows Of Harvard College | Method for characterization of nucleic acid molecules |
| US8278055B2 (en) * | 2002-05-01 | 2012-10-02 | Intel Corporation | Methods and device for analyte characterization |
| US6870361B2 (en) | 2002-12-21 | 2005-03-22 | Agilent Technologies, Inc. | System with nano-scale conductor and nano-opening |
| US7846738B2 (en) * | 2003-08-15 | 2010-12-07 | President And Fellows Of Harvard College | Study of polymer molecules and conformations with a nanopore |
| US7238485B2 (en) | 2004-03-23 | 2007-07-03 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| WO2005124888A1 (en) | 2004-06-08 | 2005-12-29 | President And Fellows Of Harvard College | Suspended carbon nanotube field effect transistor |
| DE102005013608A1 (de) * | 2005-03-24 | 2006-09-28 | Westfälische Wilhelms-Universität Münster | Vorrichtung und Verfahren für die zellfreie analytische und präparative Proteinsynthese |
| AU2008236694B2 (en) | 2007-04-04 | 2014-01-23 | The Regents Of The University Of California | Compositions, devices, systems, and methods for using a nanopore |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| US8034910B2 (en) | 2008-05-06 | 2011-10-11 | Academia Sinica, Taiwan | SUMO fusion protein expression system for producing native proteins |
| GB0820927D0 (en) * | 2008-11-14 | 2008-12-24 | Isis Innovation | Method |
| WO2010082860A1 (en) | 2009-01-19 | 2010-07-22 | Instituto De Biologia Experimental E Tecnologia (Ibet) | Method and device for nanopore based single-molecule protein/ protein interaction detection |
| GB0905140D0 (en) * | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| BRPI1012752B1 (pt) | 2009-04-20 | 2019-06-25 | Oxford Nanopore Technologies Limited | Método e aparelho para detectar uma interação de uma entidade molecular com uma proteína de membrana em uma camada de moléculas anfifílicas |
| WO2010141468A1 (en) * | 2009-06-01 | 2010-12-09 | Way Jeffrey C | Methods and molecules for yield improvement involving metabolic engineering |
| CN102741430B (zh) | 2009-12-01 | 2016-07-13 | 牛津楠路珀尔科技有限公司 | 生化分析仪器、用于进行生化分析的第一模块以及相关方法 |
| US9605307B2 (en) * | 2010-02-08 | 2017-03-28 | Genia Technologies, Inc. | Systems and methods for forming a nanopore in a lipid bilayer |
| CA3116307C (en) * | 2010-02-23 | 2023-10-17 | University Of Washington | Artificial mycolic acid membranes |
| EP2588613A4 (en) * | 2010-06-30 | 2013-12-04 | Univ Syracuse | GENERALIZED PROTEIN PORES |
-
2013
- 2013-02-15 CA CA2864824A patent/CA2864824C/en active Active
- 2013-02-15 JP JP2014557827A patent/JP6312607B2/ja active Active
- 2013-02-15 WO PCT/US2013/026414 patent/WO2013123379A2/en not_active Ceased
- 2013-02-15 EP EP13749423.3A patent/EP2814939B1/en active Active
- 2013-02-15 BR BR112014020255-9A patent/BR112014020255B1/pt active IP Right Grant
- 2013-02-15 KR KR1020147025764A patent/KR102066758B1/ko active Active
- 2013-02-15 AU AU2013221356A patent/AU2013221356B2/en active Active
- 2013-02-15 CN CN201380019821.3A patent/CN104619854B/zh active Active
- 2013-02-15 US US14/378,448 patent/US11339365B2/en active Active
- 2013-02-15 DK DK13749423.3T patent/DK2814939T3/en active
-
2017
- 2017-12-08 JP JP2017236383A patent/JP6594944B2/ja active Active
-
2022
- 2022-05-16 US US17/745,399 patent/US20220396758A1/en active Pending
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