JP2017039729A - Slit−Roboシステムを用いた骨折又は骨粗鬆症の予防或いは治療用組成物 - Google Patents
Slit−Roboシステムを用いた骨折又は骨粗鬆症の予防或いは治療用組成物 Download PDFInfo
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Abstract
Description
Claims (16)
- 下記群から選択された遺伝子又はその発現タンパク質を有効成分として含有する骨折又は骨粗鬆症の予防又は治療用薬学的組成物:
配列番号2のアミノ酸配列をコードする塩基配列からなるslit2と、
配列番号3のアミノ酸配列をコードする塩基配列からなるslit3と、
配列番号4のアミノ酸配列をコードする塩基配列からなるrobo1と、
配列番号5のアミノ酸配列をコードする塩基配列からなるrobo2。 - 前記slit2遺伝子は、配列番号10の塩基配列からなることを特徴とする請求項1に記載の薬学的組成物。
- 前記slit3遺伝子は、配列番号11の塩基配列からなることを特徴とする請求項1に記載の薬学的組成物。
- 前記robo1遺伝子は、配列番号12の塩基配列からなることを特徴とする請求項1に記載の薬学的組成物。
- 前記robo2遺伝子は、配列番号13の塩基配列からなることを特徴とする請求項1に記載の薬学的組成物。
- 前記遺伝子は、発現ベクターに挿入されていることを特徴とする請求項1に記載の薬学的組成物。
- slit2、slit3、robo1 及びrobo2で構成された群から選択されたタンパク質を有効成分として含有する骨折又は骨粗鬆症の予防あるいは改善用食品組成物。
- (a)slit2、slit3、robo1 及びrobo2で構成された群から選択された遺伝子を発現する細胞または動物を培養する段階と、
(b)被検物質を前記(a)段階の細胞または動物と接触させる段階と、
(c)前記(b)段階の細胞または動物でslit2、slit3、robo1またはrobo2タンパク質の発現の水準を、被検物質を処理しない対照群と比較する段階とを含む骨折又は骨粗鬆症治療剤のスクリーニング方法。 - 配列番号17の塩基配列によりコードされるアミノ酸配列で構成されるslit3タンパク質のLRR2ドメインの組換えペプチド。
- 請求項9による組換えペプチドを含む骨折又は骨粗鬆症の予防或いは治療用薬学的組成物。
- 下記群から選択された遺伝子の発現またはその発現タンパク質の活性を増加させる活性因子を有効成分として含む骨折又は骨粗鬆症の予防或いは治療用薬学的組成物:
配列番号2のアミノ酸配列をコードする塩基配列からなるslit2と、
配列番号3のアミノ酸配列をコードする塩基配列からなるslit3と、
配列番号4のアミノ酸配列をコードする塩基配列からなるrobo1と、
配列番号5のアミノ酸配列をコードする塩基配列からなるrobo2。 - 前記遺伝子の発現またはその発現タンパク質の活性を増加させる活性因子は、前記遺伝子またはその発現タンパク質に直接もしくは間接的に作用し、生物学的活性を改善、誘導、刺激、増加させる物質であることを特徴とする請求項11に記載の骨折又は骨粗鬆症の予防或いは治療用薬学的組成物。
- 前記物質は、有機または無機化合物と同一の単一化合物と、ペプチド、タンパク質、核酸、炭水化物及び脂質を含む生体高分子化合物と、複数の化合物の複合体とで構成された群から選択されたことを特徴とする請求項12に記載の骨折又は骨粗鬆症の予防或いは治療用薬学的組成物。
- 前記物質は、転写因子または翻訳因子であって、前記遺伝子の発現を増大させることを特徴とする請求項12に記載の骨折又は骨粗鬆症の予防或いは治療用薬学的組成物。
- 前記物質は、前記遺伝子のプロモーターに作用し、前記遺伝子の発現を誘導することを特徴とする請求項14に記載の骨折又は骨粗鬆症の予防或いは治療用薬学的組成物。
- 前記物質は、請求項15による方法でスクリーニングされたことを特徴とする請求項12乃至15のいずれか1項に記載の骨折又は骨粗鬆症の予防或いは治療用薬学的組成物。
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US10265437B2 (en) | 2014-03-14 | 2019-04-23 | The Research Foundation For The State University Of New York | Neurogenic regulation of bone growth and bone degradation |
KR101897322B1 (ko) * | 2015-01-21 | 2018-09-10 | 재단법인 아산사회복지재단 | 원발성 갑상선암 전이의 진단방법 및 이를 이용한 진단키트 |
KR102011957B1 (ko) | 2016-06-08 | 2019-08-19 | 재단법인 아산사회복지재단 | Slit-robo 시스템을 이용한 근감소증 예방 또는 치료용 조성물 |
WO2017213435A1 (ko) * | 2016-06-08 | 2017-12-14 | 재단법인 아산사회복지재단 | Slit-robo 시스템을 이용한 근감소증 예방 또는 치료용 조성물 |
CN110709415A (zh) | 2017-06-02 | 2020-01-17 | 辉瑞大药厂 | 重组robo2蛋白、组合物、方法及其用途 |
US20200397860A1 (en) * | 2018-02-14 | 2020-12-24 | Cornell University | Slit and bone growth modulation |
JP7340027B2 (ja) * | 2019-02-27 | 2023-09-06 | デウン ファーマシューティカル カンパニー リミテッド | アルブミンと結合したSlit3タンパク質のLRRD2を含む骨関連疾患の予防または治療用組成物 |
WO2024061158A1 (en) * | 2022-09-20 | 2024-03-28 | Everest Medicines (China) Co., Ltd. | Slit2 related compositions and methods |
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