JP2016537422A - ボリジ抽出物を含む代謝疾患の改善、予防または治療用組成物 - Google Patents
ボリジ抽出物を含む代謝疾患の改善、予防または治療用組成物 Download PDFInfo
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- JP2016537422A JP2016537422A JP2016553186A JP2016553186A JP2016537422A JP 2016537422 A JP2016537422 A JP 2016537422A JP 2016553186 A JP2016553186 A JP 2016553186A JP 2016553186 A JP2016553186 A JP 2016553186A JP 2016537422 A JP2016537422 A JP 2016537422A
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Abstract
【選択図】図5
Description
[実施例1:ボリジ抽出物の調製]
ボリジの葉、花、種を混合して粉砕した後、10倍体積量の精製水を混合し、これらの混合溶媒から80℃の抽出温度で6時間5回繰り返して熱水抽出した。本抽出物をろ過して真空濃縮した後、1:2でボリジとデキストリンを混合した後に噴霧乾燥してボリジ抽出物を得た。
本発明の組成物の脂肪細胞分化の抑制および細胞内の肥満関連脂質、リポタンパク質およびホルモンの測定による内臓脂肪の減少効果を確認した。
前脂肪細胞(preadipocyte)である3T3−L1細胞は、脂肪細胞の代謝過程を研究するのに広く用いられており、本発明においても、細胞の培養過程で組成物を処理した後、細胞の分化が少ないほど抗肥満効果が大きいことを示した。3T3−L1細胞を、培養液を用いて5%で二酸化炭素が供給される培養器にて37℃で培養した。細胞培養液としては、10% FBS(fetal bovine serum)と抗生物質(antibiotics)が含まれたDulbecco’s Modified Eagle’s Media(DMEM)を用いた。2〜3日の間隔で培養された細胞表面をリン酸緩衝生理食塩水(phosphate buffered saline、PBS)で洗浄した後、0.5%トリプシン(trypsin)を入れて処理し、細胞を脱着させて継代培養した。細胞は、分化誘導物質のインスリン(5μg/m)、デキサメタゾン(dexamethasone;DEX、0.25μΜ)およびMTX(l-methyl-3-methylxanthine、0.5mΜ)が含まれた分化誘導培養液に交換して1〜3日間培養し、脂肪細胞に分化を誘導した。培養3日後、インスリンのみ含有する培地で2〜3日間培養した後、インスリンを除去した培養液に取り替えた。中性脂肪に流入される葡萄糖測定実験や葡萄糖酸化実験時には10〜12日目に低濃度(5mM)葡萄糖を含有した培養液で培養し、分化誘導後にボリジ抽出物を処理した。処理された細胞は、24hr、48hrおよび72hr後に96ウェルについている細胞を、MTT分析方法を利用して細胞数を測定した。次に、ボリジ抽出物の処理後に10日間分化させた3T3−L1細胞から中性脂肪(triacylglycerol)、アディポネクチン(adiponectin)およびレプチン(leptin)の量を各々測定した。
C57BL/6Jマウスを対象に本発明の組成物を摂取するようにし、摂取前後の内臓脂肪の減少効果を確認して内臓脂肪の減少効果を確認した。
遺伝性肥満を示し、2型糖尿病が進行される動物モデルであるKK−Ayマウスを利用してボリジ抽出物の糖尿病予防効果と脂質代謝の改善効能を確認した。
老化により高血圧が誘発される高血圧動物モデルである自発性高血圧ラット(SpontaneouslyHypertensive Rat;SHR)を使い、ボリジ抽出物の抗高血圧効能を確認した。
男女に本発明の組成物を摂取するようにし、摂取前後の体重変化および体脂肪測定を通じて体重調節および肥満改善効果を確認した。
本検査に同意した満19才以上60才未満の男女50名(男性22名、女性28名)を、偽薬を摂取した対照群(placebo)と本抽出物の混合物(8:2混合比)を摂取した実験群に各25名ずつ任意に分類し、4週間偽薬または実験薬を1日400mgずつ摂取するようにした。被験者としては、体格指数(Body-Mass Index;BMI)が23以上の人またはウエスト径が男性90cm、女性80cm以上の人を選定した。摂取前と後に身長、体重、体格指数、ウエスト径、血圧、脈拍、身体組成(体脂肪率および除脂肪量−インピーダンス法)および血液検査によってHDL−コレステロール、中性脂肪および総コレステロール等を測定し、ボリジ組成物の摂取による代謝性疾患危険因子症候群の予防および改善効能評価を実施した。
本発明のボリジ抽出物摂取群と偽薬群(Placebo)のBMI(体格指数)結果は表4に示し、結果値は平均と標準偏差とで示し、統計処理はStudent’s t-testを利用し、p<0.05の時に有意であると見なして表示した。
本発明のボリジ抽出物摂取群と偽薬群(Placebo)のウエスト径を図5に示し、結果値は平均と標準偏差とで示し、統計処理はStudent’s t-testを利用し、p<0.05時に有意であると見なして表示した。
Claims (9)
- ボリジ(Borage officinalis)抽出物を有効成分として含む代謝疾患(metabolic diseases)の改善、予防または治療用組成物。
- 前記代謝疾患は、肥満、糖尿、脂質異常症(dyslipidemia)、脂肪肝およびインスリン抵抗症候群(insulin resistance syndrome)からなる群より選択される代謝疾患(metabolic diseases)であることを特徴とする、請求項1に記載の組成物。
- 前記脂質異常症は高脂血症であることを特徴とする、請求項2に記載の組成物。
- 前記インスリン抵抗症候群は、インスリン抵抗性による肥満、高血圧、動脈硬化、高脂血症、高インスリン血症および非アルコール性脂肪肝からなる群より選択される1つ以上のインスリン抵抗症候群を含むことを特徴とする、請求項2に記載の組成物。
- 前記組成物は、食品組成物、機能性食品組成物または薬剤学的組成物であることを特徴とする、請求項1に記載の組成物。
- ボリジ(Borage officinalis)抽出物を有効成分として含む組成物を対象(subject)に投与するステップを含む代謝疾患(metabolic diseases)の改善、予防または治療方法。
- 前記代謝疾患は、肥満、糖尿、脂質異常症(dyslipidemia)、脂肪肝およびインスリン抵抗症候群(insulin resistance syndrome)からなる群より選択される代謝疾患(metabolic diseases)であることを特徴とする、請求項6に記載の方法。
- 前記脂質異常症は高脂血症であることを特徴とする、請求項7に記載の方法。
- 前記インスリン抵抗症候群は、インスリン抵抗性による肥満、高血圧、動脈硬化、高脂血症、高インスリン血症および非アルコール性脂肪肝からなる群より選択される1つ以上のインスリン抵抗症候群を含むことを特徴とする、請求項7に記載の方法。
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PCT/KR2014/010132 WO2015072674A1 (ko) | 2013-11-12 | 2014-10-27 | 보리지 추출물을 포함하는 대사질환 개선, 예방 또는 치료용 조성물 |
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CN108432924A (zh) * | 2018-06-08 | 2018-08-24 | 贵州玉屏展鸿茶叶有限公司 | 一种防治糖尿病的保健茶 |
US10426806B1 (en) * | 2018-06-13 | 2019-10-01 | Muhammad Iqbal Choudhary | Anti-obesity effect of Borago officinalis linn. extracts to prevent and treat metabolic disorders |
KR102193317B1 (ko) * | 2019-03-12 | 2020-12-22 | 주식회사 엔에스웰니스 | 비만 치료 및 개선용 조성물 |
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US5776463A (en) * | 1997-02-19 | 1998-07-07 | Arginteanu; Ronit | Method of reducing stress and circulatory heart disease with freeze-dried borage petal extracts |
CN101978949A (zh) * | 2010-11-16 | 2011-02-23 | 王京南 | γ-亚麻酸酯脂肪乳静脉注射液及其制造方法 |
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US5776463A (en) * | 1997-02-19 | 1998-07-07 | Arginteanu; Ronit | Method of reducing stress and circulatory heart disease with freeze-dried borage petal extracts |
CN101978949A (zh) * | 2010-11-16 | 2011-02-23 | 王京南 | γ-亚麻酸酯脂肪乳静脉注射液及其制造方法 |
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