JP2016534731A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2016534731A5 JP2016534731A5 JP2016527338A JP2016527338A JP2016534731A5 JP 2016534731 A5 JP2016534731 A5 JP 2016534731A5 JP 2016527338 A JP2016527338 A JP 2016527338A JP 2016527338 A JP2016527338 A JP 2016527338A JP 2016534731 A5 JP2016534731 A5 JP 2016534731A5
- Authority
- JP
- Japan
- Prior art keywords
- pluripotent stem
- stem cells
- cell population
- population
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims description 42
- 210000001778 pluripotent stem cell Anatomy 0.000 claims description 31
- 210000004027 cell Anatomy 0.000 claims description 25
- 210000000130 stem cell Anatomy 0.000 claims description 13
- 238000004114 suspension culture Methods 0.000 claims description 12
- 239000000725 suspension Substances 0.000 claims description 9
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 8
- 229940098773 bovine serum albumin Drugs 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 8
- 230000004069 differentiation Effects 0.000 claims description 7
- 102000040945 Transcription factor Human genes 0.000 claims description 4
- 108091023040 Transcription factor Proteins 0.000 claims description 4
- 210000005155 neural progenitor cell Anatomy 0.000 claims description 4
- 102100039939 Growth/differentiation factor 8 Human genes 0.000 claims description 3
- 206010021143 Hypoxia Diseases 0.000 claims description 3
- 210000004381 amniotic fluid Anatomy 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 3
- 150000002632 lipids Chemical class 0.000 claims description 3
- 230000002107 myocardial effect Effects 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 210000003954 umbilical cord Anatomy 0.000 claims description 3
- 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 claims description 2
- 101000886562 Homo sapiens Growth/differentiation factor 8 Proteins 0.000 claims description 2
- 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 claims description 2
- 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 claims description 2
- 102100041030 Pancreas/duodenum homeobox protein 1 Human genes 0.000 claims description 2
- 101710183548 Pyridoxal 5'-phosphate synthase subunit PdxS Proteins 0.000 claims description 2
- 102000044880 Wnt3A Human genes 0.000 claims description 2
- 108700013515 Wnt3A Proteins 0.000 claims description 2
- 108010023082 activin A Proteins 0.000 claims description 2
- 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 claims description 2
- 210000001900 endoderm Anatomy 0.000 claims description 2
- 230000001146 hypoxic effect Effects 0.000 claims description 2
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims description 2
- 239000003112 inhibitor Substances 0.000 claims description 2
- 101150068520 wnt3a gene Proteins 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims 6
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 2
- RHUJMHOIQBDFQR-UHFFFAOYSA-N 2-[[3-(2-methoxyphenyl)-4-oxo-6,7-dihydrothieno[3,2-d]pyrimidin-2-yl]sulfanyl]-n-(6-methyl-1,3-benzothiazol-2-yl)acetamide Chemical compound COC1=CC=CC=C1N1C(=O)C(SCC2)=C2N=C1SCC(=O)NC1=NC2=CC=C(C)C=C2S1 RHUJMHOIQBDFQR-UHFFFAOYSA-N 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- 102000004877 Insulin Human genes 0.000 claims 1
- 108090001061 Insulin Proteins 0.000 claims 1
- 239000000853 adhesive Substances 0.000 claims 1
- 230000001070 adhesive effect Effects 0.000 claims 1
- 210000004413 cardiac myocyte Anatomy 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 239000008103 glucose Substances 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 230000007954 hypoxia Effects 0.000 claims 1
- 229940125396 insulin Drugs 0.000 claims 1
- 230000008186 parthenogenesis Effects 0.000 claims 1
- 239000003590 rho kinase inhibitor Substances 0.000 description 4
- 230000035519 G0 Phase Effects 0.000 description 2
- 230000010190 G1 phase Effects 0.000 description 2
- 102000043168 TGF-beta family Human genes 0.000 description 2
- 108091085018 TGF-beta family Proteins 0.000 description 2
- 230000022131 cell cycle Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000001776 parthenogenetic effect Effects 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 102100031650 C-X-C chemokine receptor type 4 Human genes 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 101000922348 Homo sapiens C-X-C chemokine receptor type 4 Proteins 0.000 description 1
- 108010056852 Myostatin Proteins 0.000 description 1
- 238000004115 adherent culture Methods 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361962158P | 2013-11-01 | 2013-11-01 | |
| US61/962,158 | 2013-11-01 | ||
| US13/998,974 US10377989B2 (en) | 2012-12-31 | 2013-12-30 | Methods for suspension cultures of human pluripotent stem cells |
| US13/998,974 | 2013-12-30 | ||
| PCT/US2014/038993 WO2015065524A2 (en) | 2013-11-01 | 2014-05-21 | Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016534731A JP2016534731A (ja) | 2016-11-10 |
| JP2016534731A5 true JP2016534731A5 (enExample) | 2017-07-06 |
Family
ID=53004904
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016527338A Pending JP2016534731A (ja) | 2013-11-01 | 2014-05-21 | 膵内分泌細胞への分化のためのヒト多能性幹細胞の懸濁及び集団化 |
| JP2016527450A Pending JP2017500013A (ja) | 2013-11-01 | 2014-06-17 | 膵内分泌細胞への分化のためのヒト多能性幹細胞の懸濁及び集団化 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016527450A Pending JP2017500013A (ja) | 2013-11-01 | 2014-06-17 | 膵内分泌細胞への分化のためのヒト多能性幹細胞の懸濁及び集団化 |
Country Status (12)
| Country | Link |
|---|---|
| EP (2) | EP3063268A4 (enExample) |
| JP (2) | JP2016534731A (enExample) |
| KR (4) | KR20160079072A (enExample) |
| CN (2) | CN105793413A (enExample) |
| AU (4) | AU2014342995C1 (enExample) |
| BR (1) | BR112016009393A8 (enExample) |
| CA (2) | CA2928639A1 (enExample) |
| MX (2) | MX2016005657A (enExample) |
| PH (2) | PH12016500783A1 (enExample) |
| RU (2) | RU2689710C2 (enExample) |
| SG (2) | SG11201603045VA (enExample) |
| WO (2) | WO2015065524A2 (enExample) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2016005657A (es) * | 2013-11-01 | 2017-01-05 | Janssen Biotech Inc | Suspensión y agrupamiento de células madre humanas pluripotentes para la diferenciación a células endocrinas pancreáticas. |
| WO2017223233A1 (en) * | 2016-06-23 | 2017-12-28 | Tithon Biotech, Inc. | Cells expressing parathyroid hormone 1 receptor and uses thereof |
| JP7088564B2 (ja) | 2016-11-10 | 2022-06-21 | ヴィアサイト，インコーポレイテッド | 細胞送達装置におけるｐｄｘ１膵臓内胚葉細胞及びその方法 |
| CA3042562C (en) * | 2016-11-16 | 2023-02-28 | Cynata Therapeutics Limited | Pluripotent stem cell assay |
| US10391156B2 (en) | 2017-07-12 | 2019-08-27 | Viacyte, Inc. | University donor cells and related methods |
| EP4488363A3 (en) * | 2017-07-21 | 2025-03-26 | Vertex Pharmaceuticals Incorporated | Re-aggregation of stem cell-derived pancreatic beta cells |
| JP7000896B2 (ja) * | 2017-08-23 | 2022-01-19 | 王子ホールディングス株式会社 | 細胞シート形成部材、細胞シート形成部材の製造方法、および、細胞シートの製造方法 |
| MX2020007071A (es) * | 2018-01-05 | 2020-11-11 | Platelet Biogenesis Inc | Composiciones y metodos para producir megacariocitos. |
| US12104173B2 (en) | 2018-01-18 | 2024-10-01 | Agency For Science, Technology And Research | Method for differentiation of human pluripotent stem cell lines in suspension culture |
| KR102115360B1 (ko) * | 2018-05-30 | 2020-05-26 | 주식회사 바이블리오테카 | 성체 줄기세포 배양액 및 그 제조방법 |
| US10724052B2 (en) | 2018-09-07 | 2020-07-28 | Crispr Therapeutics Ag | Universal donor cells |
| EP3975926A1 (en) | 2019-05-31 | 2022-04-06 | W.L. Gore & Associates, Inc. | A biocompatible membrane composite |
| EP3976237A1 (en) | 2019-05-31 | 2022-04-06 | W.L. Gore & Associates Inc. | Cell encapsulation devices with controlled oxygen diffusion distances |
| AU2020283056B2 (en) | 2019-05-31 | 2023-06-08 | Viacyte, Inc. | A biocompatible membrane composite |
| CA3139591C (en) | 2019-05-31 | 2024-01-16 | Timothy M. BRUHN | A biocompatible membrane composite |
| JP2022547417A (ja) * | 2019-08-27 | 2022-11-14 | ユナイテッド セラピューティクス コーポレイション | 肺胞細胞を培養するための方法および組成物 |
| US11118195B2 (en) | 2019-09-05 | 2021-09-14 | Crispr Therapeutics Ag | Universal donor cells |
| AU2020340622A1 (en) | 2019-09-05 | 2022-03-03 | Crispr Therapeutics Ag | Universal donor cells |
| EP4061929A1 (en) * | 2019-11-22 | 2022-09-28 | Novo Nordisk A/S | Spin-aggregated neural microspheres and the application thereof |
| AU2021414405A1 (en) | 2020-12-31 | 2023-08-10 | Crispr Therapeutics Ag | Universal donor cells |
| WO2022203051A1 (ja) * | 2021-03-25 | 2022-09-29 | 株式会社カネカ | 多能性幹細胞集団の製造方法 |
| CN116218766A (zh) * | 2023-04-21 | 2023-06-06 | 北京意胜生物科技有限公司 | 一种制备胰腺前体细胞的方法、培养基及其应用 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005533480A (ja) * | 2001-11-09 | 2005-11-10 | アーテセル・サイエンシズ・インコーポレーテツド | 脂肪組織由来間質細胞の膵内分泌分化およびその使用 |
| DK3441459T3 (da) * | 2006-08-02 | 2021-06-07 | Technion Res & Dev Foundation | Fremgangsmåder til ekspansion af embryonale stamceller i en suspensionskultur |
| EP2088190A4 (en) * | 2006-11-09 | 2011-01-05 | Japan Government | METHOD FOR THE CULTURE AND PASSING OF AN EMBRYONAL PRIMATIVE STEM CELL AND METHOD FOR INDUCING THE DIFFERENTIATION OF THE EMBRYONAL STEM CELL |
| US20090053182A1 (en) * | 2007-05-25 | 2009-02-26 | Medistem Laboratories, Inc. | Endometrial stem cells and methods of making and using same |
| WO2009006399A1 (en) * | 2007-07-01 | 2009-01-08 | Lifescan | Single pluripotent stem cell culture |
| JP2011514169A (ja) * | 2008-03-17 | 2011-05-06 | エージェンシー フォー サイエンス，テクノロジー アンド リサーチ | 幹細胞培養のためのマイクロキャリア |
| AU2009267137A1 (en) * | 2008-06-30 | 2010-01-07 | Centocor Ortho Biotech Inc. | Differentiation of pluripotent stem cells |
| US8008075B2 (en) * | 2008-11-04 | 2011-08-30 | Viacyte, Inc. | Stem cell aggregate suspension compositions and methods of differentiation thereof |
| CA2770753C (en) * | 2009-08-12 | 2019-01-15 | Kyoto University | Method for inducing differentiation of pluripotent stem cells into neural precursor cells |
| JP5936134B2 (ja) * | 2010-06-15 | 2016-06-15 | 国立大学法人京都大学 | ヒト人工多能性幹細胞の選択方法 |
| KR101836850B1 (ko) * | 2010-08-31 | 2018-03-09 | 얀센 바이오테크 인코포레이티드 | 인간 배아 줄기 세포의 분화 |
| MY177150A (en) * | 2011-02-28 | 2020-09-08 | Stempeutics Res Malaysia Sdn Bhd | Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof |
| WO2013056072A1 (en) * | 2011-10-13 | 2013-04-18 | Wisconsin Alumni Research Foundation | Generation of cardiomyocytes from human pluripotent stem cells |
| AU2012355698B2 (en) * | 2011-12-22 | 2018-11-29 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into single hormonal insulin positive cells |
| US10519422B2 (en) * | 2012-02-29 | 2019-12-31 | Riken | Method of producing human retinal pigment epithelial cells |
| EP4039798A1 (en) * | 2012-12-31 | 2022-08-10 | Janssen Biotech, Inc. | Suspension and clustering of human pluripotent cells |
| MX2016005657A (es) * | 2013-11-01 | 2017-01-05 | Janssen Biotech Inc | Suspensión y agrupamiento de células madre humanas pluripotentes para la diferenciación a células endocrinas pancreáticas. |
-
2014
- 2014-05-21 MX MX2016005657A patent/MX2016005657A/es unknown
- 2014-05-21 KR KR1020167014409A patent/KR20160079072A/ko not_active Ceased
- 2014-05-21 WO PCT/US2014/038993 patent/WO2015065524A2/en not_active Ceased
- 2014-05-21 CN CN201480059910.5A patent/CN105793413A/zh active Pending
- 2014-05-21 KR KR1020187034291A patent/KR20180128529A/ko not_active Abandoned
- 2014-05-21 JP JP2016527338A patent/JP2016534731A/ja active Pending
- 2014-05-21 AU AU2014342995A patent/AU2014342995C1/en not_active Ceased
- 2014-05-21 EP EP14857034.4A patent/EP3063268A4/en not_active Withdrawn
- 2014-05-21 SG SG11201603045VA patent/SG11201603045VA/en unknown
- 2014-05-21 RU RU2016121409A patent/RU2689710C2/ru active
- 2014-05-21 CA CA2928639A patent/CA2928639A1/en not_active Abandoned
- 2014-06-17 JP JP2016527450A patent/JP2017500013A/ja active Pending
- 2014-06-17 BR BR112016009393A patent/BR112016009393A8/pt not_active Application Discontinuation
- 2014-06-17 KR KR1020187034483A patent/KR20180130001A/ko not_active Withdrawn
- 2014-06-17 CA CA2928741A patent/CA2928741A1/en not_active Abandoned
- 2014-06-17 EP EP14859059.9A patent/EP3063269A4/en not_active Withdrawn
- 2014-06-17 RU RU2016121404A patent/RU2016121404A/ru not_active Application Discontinuation
- 2014-06-17 WO PCT/US2014/042796 patent/WO2015065537A1/en not_active Ceased
- 2014-06-17 SG SG11201603047PA patent/SG11201603047PA/en unknown
- 2014-06-17 KR KR1020167014406A patent/KR20160079071A/ko not_active Abandoned
- 2014-06-17 MX MX2016005658A patent/MX2016005658A/es unknown
- 2014-06-17 AU AU2014343007A patent/AU2014343007C1/en not_active Ceased
- 2014-06-17 CN CN201480059902.0A patent/CN105683362A/zh active Pending
-
2016
- 2016-04-27 PH PH12016500783A patent/PH12016500783A1/en unknown
- 2016-04-27 PH PH12016500782A patent/PH12016500782A1/en unknown
-
2018
- 2018-07-26 AU AU2018208717A patent/AU2018208717A1/en not_active Abandoned
- 2018-07-26 AU AU2018208707A patent/AU2018208707A1/en not_active Abandoned
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2016534731A5 (enExample) | ||
| RU2016121409A (ru) | Суспендирование и кластеризация плюрипотентных стволовых клеток человека с целью их дифференцировки в панкреатические эндокринные клетки | |
| ES2610812T3 (es) | Cultivo de células madre pluripotentes | |
| JP2017500013A5 (enExample) | ||
| Nie et al. | Scalable culture and cryopreservation of human embryonic stem cells on microcarriers | |
| JP2015516161A5 (enExample) | ||
| Lindenmair et al. | Mesenchymal stem or stromal cells from amnion and umbilical cord tissue and their potential for clinical applications | |
| RU2015131839A (ru) | Суспендирование и кластеризация плюрипотентных клеток человека с целью их дифференцировки в панкреатические эндокринные клетки | |
| Shafa et al. | Human-induced pluripotent stem cells manufactured using a current good manufacturing practice-compliant process differentiate into clinically relevant cells from three germ layers | |
| JP2010532172A (ja) | 単細胞の分化万能性幹細胞の培養 | |
| EP2823037A1 (en) | Defined media for expansion and maintenance of pluripotent stem cells | |
| JP2015509381A5 (enExample) | ||
| Tran et al. | Efficient differentiation of human pluripotent stem cells into mesenchymal stem cells by modulating intracellular signaling pathways in a feeder/serum-free system | |
| Chin et al. | Defined and serum-free media support undifferentiated human embryonic stem cell growth | |
| WO2018138281A8 (en) | Pluripotent stem cells | |
| JPWO2015147047A1 (ja) | 多能性幹細胞培養用培地 | |
| Burrell et al. | Stirred suspension bioreactor culture of porcine induced pluripotent stem cells | |
| US20150275179A1 (en) | Single Pluripotent Stem Cell Culture | |
| Simpson et al. | Engineering patient-specific valves using stem cells generated from skin biopsy specimens | |
| WO2013141731A8 (en) | Process for ex-vivo expansion of hematopoietic stem cells in a bioreactor | |
| JP2025511790A (ja) | ｉＰＳＣ由来ＨＳＣ及び子孫の大規模製造 | |
| Aalders et al. | Robust protocol for feeder-free adaptation of cryopreserved human pluripotent stem cells | |
| Klimanskaya | Embryonic stem cells: derivation, properties, and challenges | |
| CN104988115A (zh) | 喹啉或异喹啉类小分子化合物在人多能干细胞培养及冷冻保存中的应用 | |
| Baek et al. | Clean-up human embryonic stem cell lines using humanized culture condition |