CN105793413A - 用于分化成胰腺内分泌细胞的人多能干细胞的悬浮和群集 - Google Patents

用于分化成胰腺内分泌细胞的人多能干细胞的悬浮和群集 Download PDF

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Publication number
CN105793413A
CN105793413A CN201480059910.5A CN201480059910A CN105793413A CN 105793413 A CN105793413 A CN 105793413A CN 201480059910 A CN201480059910 A CN 201480059910A CN 105793413 A CN105793413 A CN 105793413A
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cells
cell
differentiation
pluripotent
expression
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CN201480059910.5A
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Chinese (zh)
Inventor
B.弗赖尔
D.拉尼奥斯卡斯
M.布拉克莫雷
H.王
K.里洛瓦
S.内尔森
E.罗索查
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Janssen Biotech Inc
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Centocor Ortho Biotech Inc
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Priority claimed from US13/998,974 external-priority patent/US10377989B2/en
Application filed by Centocor Ortho Biotech Inc filed Critical Centocor Ortho Biotech Inc
Publication of CN105793413A publication Critical patent/CN105793413A/zh
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CN201480059910.5A 2013-11-01 2014-05-21 用于分化成胰腺内分泌细胞的人多能干细胞的悬浮和群集 Pending CN105793413A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361962158P 2013-11-01 2013-11-01
US61/962158 2013-11-01
US13/998,974 US10377989B2 (en) 2012-12-31 2013-12-30 Methods for suspension cultures of human pluripotent stem cells
US13/998974 2013-12-30
PCT/US2014/038993 WO2015065524A2 (en) 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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Publication Number Publication Date
CN105793413A true CN105793413A (zh) 2016-07-20

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CN201480059910.5A Pending CN105793413A (zh) 2013-11-01 2014-05-21 用于分化成胰腺内分泌细胞的人多能干细胞的悬浮和群集
CN201480059902.0A Pending CN105683362A (zh) 2013-11-01 2014-06-17 用于分化成胰腺内分泌细胞的人多能干细胞的悬浮和群集

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CN201480059902.0A Pending CN105683362A (zh) 2013-11-01 2014-06-17 用于分化成胰腺内分泌细胞的人多能干细胞的悬浮和群集

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EP (2) EP3063268A4 (enExample)
JP (2) JP2016534731A (enExample)
KR (4) KR20160079072A (enExample)
CN (2) CN105793413A (enExample)
AU (4) AU2014342995C1 (enExample)
BR (1) BR112016009393A8 (enExample)
CA (2) CA2928639A1 (enExample)
MX (2) MX2016005657A (enExample)
PH (2) PH12016500782A1 (enExample)
RU (2) RU2689710C2 (enExample)
SG (2) SG11201603045VA (enExample)
WO (2) WO2015065524A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116218766A (zh) * 2023-04-21 2023-06-06 北京意胜生物科技有限公司 一种制备胰腺前体细胞的方法、培养基及其应用
TWI810202B (zh) * 2017-08-23 2023-08-01 日商王子控股股份有限公司 細胞層片形成構件、細胞層片形成構件的製造方法、及細胞層片的製造方法

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2689710C2 (ru) * 2013-11-01 2019-05-29 Янссен Байотек, Инк. Суспендирование и кластеризация плюрипотентных стволовых клеток человека с целью их дифференцировки в панкреатические эндокринные клетки
RU2771328C2 (ru) * 2016-06-23 2022-04-29 Титон Биотек, Инк. Клетки, экспрессирующие рецептор паратиреоидного гормона 1, и их применение
CN118924958A (zh) 2016-11-10 2024-11-12 韦尔赛特公司 含有pdx1胰腺内胚层细胞的细胞递送装置及其方法
AU2017361117B9 (en) 2016-11-16 2022-03-24 Cynata Therapeutics Limited Pluripotent stem cell assay
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
EP4488363A3 (en) * 2017-07-21 2025-03-26 Vertex Pharmaceuticals Incorporated Re-aggregation of stem cell-derived pancreatic beta cells
AU2019205942B2 (en) * 2018-01-05 2025-05-15 Platelet Biogenesis, Inc. Compositions and methods for producing megakaryocytes
JP2021510527A (ja) * 2018-01-18 2021-04-30 エージェンシー フォー サイエンス， テクノロジー アンド リサーチ 懸濁培養におけるヒト多能性幹細胞系の分化のための方法
KR102115360B1 (ko) * 2018-05-30 2020-05-26 주식회사 바이블리오테카 성체 줄기세포 배양액 및 그 제조방법
US12378572B2 (en) 2018-09-07 2025-08-05 Crispr Therapeutics Ag Universal donor cells
CA3139292C (en) 2019-05-31 2025-06-10 Viacyte, Inc. CELL ENCAPSULATION DEVICES WITH REGULATED OXYGEN DIFFUSION DISTANCES
WO2020243666A1 (en) 2019-05-31 2020-12-03 W. L. Gore & Associates, Inc. A biocompatible membrane composite
AU2020282355B2 (en) 2019-05-31 2023-11-02 Viacyte, Inc. A biocompatible membrane composite
AU2020283056B2 (en) 2019-05-31 2023-06-08 Viacyte, Inc. A biocompatible membrane composite
KR20220090496A (ko) * 2019-08-27 2022-06-29 유나이티드 쎄러퓨틱스 코포레이션 폐포 세포를 배양하기 위한 방법 및 조성물
US11118196B2 (en) 2019-09-05 2021-09-14 Crispr Therapeutics Ag Universal donor cells
KR20220058579A (ko) 2019-09-05 2022-05-09 크리스퍼 테라퓨틱스 아게 보편적 공여자 세포
WO2021099532A1 (en) * 2019-11-22 2021-05-27 Novo Nordisk A/S Spin-aggregated neural microspheres and the application thereof
WO2022144856A1 (en) 2020-12-31 2022-07-07 Crispr Therapeutics Ag Universal donor cells
US20240191185A1 (en) * 2021-03-25 2024-06-13 Kaneka Corporation Method for producing pluripotent stem cell population
JP2022151855A (ja) * 2021-03-25 2022-10-07 株式会社カネカ 多能性幹細胞集団の製造方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130189777A1 (en) * 2011-12-22 2013-07-25 Janssen Biotech, Inc. Differentiation of Human Embryonic Stem Cells into Single Hormonal Insulin Positive Cells

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030124721A1 (en) * 2001-11-09 2003-07-03 Bentley Cheatham Endocrine pancreas differentiation of adipose tissue-derived stromal cells and uses thereof
PT2733203T (pt) * 2006-08-02 2019-01-23 Technion Res & Dev Foundation Métodos de expansão de células estminais embrionárias numa cultura de suspensão
JP5067949B2 (ja) * 2006-11-09 2012-11-07 独立行政法人国立国際医療研究センター 霊長類動物胚性幹細胞の培養及び継代方法、並びにその分化誘導方法
US20090053182A1 (en) * 2007-05-25 2009-02-26 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
CN104250632B (zh) * 2007-07-01 2018-09-11 生命扫描有限公司 单个多能干细胞培养
WO2009116951A2 (en) * 2008-03-17 2009-09-24 Agency For Science, Technology And Research Microcarriers for stem cell culture
US20100015711A1 (en) * 2008-06-30 2010-01-21 Janet Davis Differentiation of Pluripotent Stem Cells
US8008075B2 (en) * 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
AU2010283229B2 (en) * 2009-08-12 2015-04-02 Kyoto University Method for inducing differentiation of pluripotent stem cells into neural precursor cells
WO2011158960A1 (en) * 2010-06-15 2011-12-22 Kyoto University Method for selecting human induced pluripotent stem cells
CN108517310B (zh) * 2010-08-31 2022-02-15 詹森生物科技公司 人胚胎干细胞的分化
WO2012117333A1 (en) * 2011-02-28 2012-09-07 Stempeutics Research Malaysia Sdn Bhd Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof
WO2013056072A1 (en) * 2011-10-13 2013-04-18 Wisconsin Alumni Research Foundation Generation of cardiomyocytes from human pluripotent stem cells
US10519422B2 (en) * 2012-02-29 2019-12-31 Riken Method of producing human retinal pigment epithelial cells
WO2014106141A1 (en) * 2012-12-31 2014-07-03 Janssen Biotech, Inc. Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells
RU2689710C2 (ru) * 2013-11-01 2019-05-29 Янссен Байотек, Инк. Суспендирование и кластеризация плюрипотентных стволовых клеток человека с целью их дифференцировки в панкреатические эндокринные клетки

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130189777A1 (en) * 2011-12-22 2013-07-25 Janssen Biotech, Inc. Differentiation of Human Embryonic Stem Cells into Single Hormonal Insulin Positive Cells

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ROWLEY等: "Meeting Lot-Size Challenges of Manufacturing Adherent Cells for Therapy", 《BIOPROCESS》 *
WANT等: "Large-scale expansion and exploitation of pluripotent stem cells for regenerative medicine purposes: beyond the T flask", 《REGEN MED》 *
YANG等: "Evaluation of human MSCs cell cycle,viability and differentiation in micromass culture", 《BIORHEOLOGY》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI810202B (zh) * 2017-08-23 2023-08-01 日商王子控股股份有限公司 細胞層片形成構件、細胞層片形成構件的製造方法、及細胞層片的製造方法
CN116218766A (zh) * 2023-04-21 2023-06-06 北京意胜生物科技有限公司 一种制备胰腺前体细胞的方法、培养基及其应用

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