CN105793413A - 用于分化成胰腺内分泌细胞的人多能干细胞的悬浮和群集 - Google Patents

用于分化成胰腺内分泌细胞的人多能干细胞的悬浮和群集 Download PDF

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CN105793413A
CN105793413A CN201480059910.5A CN201480059910A CN105793413A CN 105793413 A CN105793413 A CN 105793413A CN 201480059910 A CN201480059910 A CN 201480059910A CN 105793413 A CN105793413 A CN 105793413A
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cells
cell
differentiation
pluripotent
expression
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CN201480059910.5A
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Chinese (zh)
Inventor
B.弗赖尔
D.拉尼奥斯卡斯
M.布拉克莫雷
H.王
K.里洛瓦
S.内尔森
E.罗索查
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Janssen Biotech Inc
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Centocor Ortho Biotech Inc
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Priority claimed from US13/998,974 external-priority patent/US10377989B2/en
Application filed by Centocor Ortho Biotech Inc filed Critical Centocor Ortho Biotech Inc
Publication of CN105793413A publication Critical patent/CN105793413A/zh
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CN201480059910.5A 2013-11-01 2014-05-21 用于分化成胰腺内分泌细胞的人多能干细胞的悬浮和群集 Pending CN105793413A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361962158P 2013-11-01 2013-11-01
US61/962158 2013-11-01
US13/998974 2013-12-30
US13/998,974 US10377989B2 (en) 2012-12-31 2013-12-30 Methods for suspension cultures of human pluripotent stem cells
PCT/US2014/038993 WO2015065524A2 (en) 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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Publication Number Publication Date
CN105793413A true CN105793413A (zh) 2016-07-20

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CN201480059910.5A Pending CN105793413A (zh) 2013-11-01 2014-05-21 用于分化成胰腺内分泌细胞的人多能干细胞的悬浮和群集
CN201480059902.0A Pending CN105683362A (zh) 2013-11-01 2014-06-17 用于分化成胰腺内分泌细胞的人多能干细胞的悬浮和群集

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EP (2) EP3063268A4 (enExample)
JP (2) JP2016534731A (enExample)
KR (4) KR20180128529A (enExample)
CN (2) CN105793413A (enExample)
AU (4) AU2014342995C1 (enExample)
BR (1) BR112016009393A8 (enExample)
CA (2) CA2928639A1 (enExample)
MX (2) MX2016005657A (enExample)
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116218766A (zh) * 2023-04-21 2023-06-06 北京意胜生物科技有限公司 一种制备胰腺前体细胞的方法、培养基及其应用
TWI810202B (zh) * 2017-08-23 2023-08-01 日商王子控股股份有限公司 細胞層片形成構件、細胞層片形成構件的製造方法、及細胞層片的製造方法

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2014342995C1 (en) * 2013-11-01 2018-08-09 Janssen Biotech, Inc. Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells
KR20230067696A (ko) * 2016-06-23 2023-05-16 티톤 바이오테크, 인크. 부갑상선 호르몬 1 수용체를 발현하는 세포 및 그의 용도
EP4595951A3 (en) 2016-11-10 2025-08-27 ViaCyte, Inc. Pdx1 pancreatic endoderm cells in cell delivery devices
US11591571B2 (en) 2016-11-16 2023-02-28 Cynata Therapeutics Limited Pluripotent stem cell assay
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
AU2018304703A1 (en) * 2017-07-21 2020-03-12 Vertex Pharmaceuticals Incorporated Re-aggregation of stem cell-derived pancreatic beta cells
JP2021509812A (ja) * 2018-01-05 2021-04-08 プレートレット バイオジェネシス, インコーポレイテッド 巨核球を産生するための組成物および方法
SG11202006731VA (en) 2018-01-18 2020-08-28 Agency Science Tech & Res Method for differentiation of human pluripotent stem cell lines in suspension culture
KR102115360B1 (ko) * 2018-05-30 2020-05-26 주식회사 바이블리오테카 성체 줄기세포 배양액 및 그 제조방법
US12378572B2 (en) 2018-09-07 2025-08-05 Crispr Therapeutics Ag Universal donor cells
CA3257300A1 (en) 2019-05-31 2025-06-12 W. L. Gore & Associates, Inc. Cell encapsulation devices with controlled oxygen diffusion distances
WO2020243663A1 (en) 2019-05-31 2020-12-03 W. L. Gore & Associates, Inc. A biocompatible membrane composite
US20220233298A1 (en) 2019-05-31 2022-07-28 W. L. Gore & Associates, Inc. A biocompatible membrane composite
AU2020283056B2 (en) 2019-05-31 2023-06-08 Viacyte, Inc. A biocompatible membrane composite
AU2020337921A1 (en) * 2019-08-27 2022-03-17 United Therapeutics Corporation Methods and compositions for culturing alveolar cells
EP4025224A1 (en) 2019-09-05 2022-07-13 CRISPR Therapeutics AG Universal donor cells
JP7753197B2 (ja) 2019-09-05 2025-10-14 クリスパー セラピューティクス アクチェンゲゼルシャフト ユニバーサルドナー細胞
WO2021099532A1 (en) * 2019-11-22 2021-05-27 Novo Nordisk A/S Spin-aggregated neural microspheres and the application thereof
KR20230146007A (ko) 2020-12-31 2023-10-18 크리스퍼 테라퓨틱스 아게 범용 공여자 세포
WO2022203051A1 (ja) * 2021-03-25 2022-09-29 株式会社カネカ 多能性幹細胞集団の製造方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130189777A1 (en) * 2011-12-22 2013-07-25 Janssen Biotech, Inc. Differentiation of Human Embryonic Stem Cells into Single Hormonal Insulin Positive Cells

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0213805A (pt) * 2001-11-09 2005-08-16 Artecel Sciences Inc Células estromais derivadas de tecido adiposo para diferenciação endócrina do pâncreas e usos das mesmas
EP2733203B1 (en) * 2006-08-02 2018-10-10 Technion Research & Development Foundation Ltd. Methods of expanding embryonic stem cells in a suspension culture
US20110151554A1 (en) * 2006-11-09 2011-06-23 Akira Yuo Method for culturing and subculturing primate embryonic stem cell, as well as method for inducing differentiation thereof
WO2008148105A1 (en) * 2007-05-25 2008-12-04 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
RU2473687C2 (ru) * 2007-07-01 2013-01-27 Лайфскен, Инк. Культивирование отдельных эмбриональных стволовых клеток
WO2009116951A2 (en) * 2008-03-17 2009-09-24 Agency For Science, Technology And Research Microcarriers for stem cell culture
ES2697798T3 (es) * 2008-06-30 2019-01-28 Janssen Biotech Inc Diferenciación de células madre pluripotentes
US8008075B2 (en) * 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
WO2011019092A1 (en) * 2009-08-12 2011-02-17 Kyoto University Method for inducing differentiation of pluripotent stem cells into neural precursor cells
US8932857B2 (en) * 2010-06-15 2015-01-13 Kyoto University Method for selecting reduced differentiation resistance human induced pluripotent stem cells
ES2659393T3 (es) * 2010-08-31 2018-03-15 Janssen Biotech, Inc. Diferenciación de células madre embrionarias humanas
MY177150A (en) * 2011-02-28 2020-09-08 Stempeutics Res Malaysia Sdn Bhd Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof
WO2013056072A1 (en) * 2011-10-13 2013-04-18 Wisconsin Alumni Research Foundation Generation of cardiomyocytes from human pluripotent stem cells
US10519422B2 (en) * 2012-02-29 2019-12-31 Riken Method of producing human retinal pigment epithelial cells
CN105705634A (zh) * 2012-12-31 2016-06-22 詹森生物科技公司 用于分化成胰腺内分泌细胞的人多能细胞的悬浮和群集
AU2014342995C1 (en) * 2013-11-01 2018-08-09 Janssen Biotech, Inc. Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130189777A1 (en) * 2011-12-22 2013-07-25 Janssen Biotech, Inc. Differentiation of Human Embryonic Stem Cells into Single Hormonal Insulin Positive Cells

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ROWLEY等: "Meeting Lot-Size Challenges of Manufacturing Adherent Cells for Therapy", 《BIOPROCESS》 *
WANT等: "Large-scale expansion and exploitation of pluripotent stem cells for regenerative medicine purposes: beyond the T flask", 《REGEN MED》 *
YANG等: "Evaluation of human MSCs cell cycle,viability and differentiation in micromass culture", 《BIORHEOLOGY》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI810202B (zh) * 2017-08-23 2023-08-01 日商王子控股股份有限公司 細胞層片形成構件、細胞層片形成構件的製造方法、及細胞層片的製造方法
CN116218766A (zh) * 2023-04-21 2023-06-06 北京意胜生物科技有限公司 一种制备胰腺前体细胞的方法、培养基及其应用

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