BR112016009393A8 - método para produzir um agrupamento (cluster) de células tridimensional, e método não enzimático para iniciar a cultura de suspensão de células pluripotentes a partir de uma cultura aderente ou planar - Google Patents

método para produzir um agrupamento (cluster) de células tridimensional, e método não enzimático para iniciar a cultura de suspensão de células pluripotentes a partir de uma cultura aderente ou planar Download PDF

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BR112016009393A8
BR112016009393A8 BR112016009393A BR112016009393A BR112016009393A8 BR 112016009393 A8 BR112016009393 A8 BR 112016009393A8 BR 112016009393 A BR112016009393 A BR 112016009393A BR 112016009393 A BR112016009393 A BR 112016009393A BR 112016009393 A8 BR112016009393 A8 BR 112016009393A8
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culture
adherent
initiating
producing
cells
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BR112016009393A
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Fryer Benjamin
Laniauskas Daina
Rosocha Elizabeth
Wang Haiyun
Lilova Kostadinka
Blackmoore Marcia
Nelson Shelley
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Janssen Biotech Inc
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Priority claimed from US13/998,974 external-priority patent/US10377989B2/en
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of BR112016009393A8 publication Critical patent/BR112016009393A8/pt

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Abstract

a presente invenção fornece métodos para preparar agrupamentos de células-tronco pluripotentes agregadas para diferenciação. especificamente, a invenção revela métodos de diferenciação de células pluripotentes em linhagens de células beta, células cardíacas e células neuronais usando agrupamento de suspensão. os métodos envolvem preparar os agrupamentos de células agregadas e a subsequente diferenciação desses agrupamentos.
BR112016009393A 2013-11-01 2014-06-17 método para produzir um agrupamento (cluster) de células tridimensional, e método não enzimático para iniciar a cultura de suspensão de células pluripotentes a partir de uma cultura aderente ou planar BR112016009393A8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361962158P 2013-11-01 2013-11-01
US13/998,974 US10377989B2 (en) 2012-12-31 2013-12-30 Methods for suspension cultures of human pluripotent stem cells
PCT/US2014/042796 WO2015065537A1 (en) 2013-11-01 2014-06-17 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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Publication Number Publication Date
BR112016009393A8 true BR112016009393A8 (pt) 2020-03-24

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BR112016009393A BR112016009393A8 (pt) 2013-11-01 2014-06-17 método para produzir um agrupamento (cluster) de células tridimensional, e método não enzimático para iniciar a cultura de suspensão de células pluripotentes a partir de uma cultura aderente ou planar

Country Status (12)

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EP (2) EP3063268A4 (pt)
JP (2) JP2016534731A (pt)
KR (4) KR20180128529A (pt)
CN (2) CN105793413A (pt)
AU (4) AU2014342995C1 (pt)
BR (1) BR112016009393A8 (pt)
CA (2) CA2928639A1 (pt)
MX (2) MX2016005657A (pt)
PH (2) PH12016500783A1 (pt)
RU (2) RU2689710C2 (pt)
SG (2) SG11201603045VA (pt)
WO (2) WO2015065524A2 (pt)

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CN105793413A (zh) 2016-07-20
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