EP3162812A4 - Mutant hemagglutinin complex protein, and method for culturing pluripotent stem cells using same - Google Patents
Mutant hemagglutinin complex protein, and method for culturing pluripotent stem cells using same Download PDFInfo
- Publication number
- EP3162812A4 EP3162812A4 EP15811956.0A EP15811956A EP3162812A4 EP 3162812 A4 EP3162812 A4 EP 3162812A4 EP 15811956 A EP15811956 A EP 15811956A EP 3162812 A4 EP3162812 A4 EP 3162812A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- stem cells
- same
- pluripotent stem
- complex protein
- culturing pluripotent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 101710154606 Hemagglutinin Proteins 0.000 title 1
- 101710093908 Outer capsid protein VP4 Proteins 0.000 title 1
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 title 1
- 101710176177 Protein A56 Proteins 0.000 title 1
- 238000012258 culturing Methods 0.000 title 1
- 239000000185 hemagglutinin Substances 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 210000001778 pluripotent stem cell Anatomy 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0081—Purging biological preparations of unwanted cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/72—Undefined extracts from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases (EC 2.)
- C12N2501/727—Kinases (EC 2.7.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/998—Proteins not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014133364 | 2014-06-27 | ||
| PCT/JP2015/068715 WO2015199243A1 (en) | 2014-06-27 | 2015-06-29 | Mutant hemagglutinin complex protein, and method for culturing pluripotent stem cells using same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP3162812A1 EP3162812A1 (en) | 2017-05-03 |
| EP3162812A4 true EP3162812A4 (en) | 2018-02-28 |
Family
ID=54938318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP15811956.0A Pending EP3162812A4 (en) | 2014-06-27 | 2015-06-29 | Mutant hemagglutinin complex protein, and method for culturing pluripotent stem cells using same |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US11230701B2 (en) |
| EP (1) | EP3162812A4 (en) |
| JP (1) | JP6355736B2 (en) |
| KR (1) | KR20170020431A (en) |
| CN (1) | CN106459223A (en) |
| AU (1) | AU2015281030A1 (en) |
| BR (1) | BR112016030510A2 (en) |
| CA (1) | CA2953670A1 (en) |
| SG (1) | SG11201610878QA (en) |
| WO (1) | WO2015199243A1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016121840A1 (en) * | 2015-01-29 | 2016-08-04 | 国立大学法人 東京大学 | Cell culture method, cell aggregates, cell aggregation control agent, and medium |
| JP6949349B2 (en) * | 2016-12-20 | 2021-10-13 | 国立大学法人大阪大学 | A composition for promoting the proliferation of pluripotent stem cells, and a method for promoting the proliferation of pluripotent stem cells. |
| JP7269605B2 (en) * | 2017-11-24 | 2023-05-09 | 国立大学法人金沢大学 | Hemagglutinin complex protein and use thereof |
| WO2023149565A1 (en) * | 2022-02-07 | 2023-08-10 | 国立大学法人大阪大学 | Method for inducing differentiation of pluripotent stem cells into retinal pigment epithelial cells |
| WO2023149566A1 (en) * | 2022-02-07 | 2023-08-10 | 国立大学法人大阪大学 | Differentiation induction control agent and differentiation induction stabilizing agent for pluripotent stem cells |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110053244A1 (en) * | 2009-08-27 | 2011-03-03 | Oyler George A | NOVEL PROTEIN DELIVERY SYSTEM TO GENERATE INDUCED PLURIPOTENT STEM (iPS) CELLS OR TISSUE-SPECIFIC CELLS |
| WO2013013105A2 (en) * | 2011-07-19 | 2013-01-24 | Vivoscript,Inc. | Compositions and methods for re-programming cells without genetic modification for repairing cartilage damage |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7172764B2 (en) | 2003-11-17 | 2007-02-06 | Allergan, Inc. | Rescue agents for treating botulinum toxin intoxications |
| JP2008169166A (en) * | 2007-01-14 | 2008-07-24 | Tokyo Univ Of Agriculture & Technology | Sugar-binding polypeptide, composite material and drug delivery system |
| JP2012143229A (en) | 2010-12-24 | 2012-08-02 | Chiba Univ | Composition for culturing pluripotent stem cell and use thereof |
| EP2929893B1 (en) * | 2012-12-04 | 2020-10-14 | Osaka University | Adjuvant for mucous membrane vaccine |
| SG11201505155WA (en) * | 2012-12-28 | 2015-08-28 | Univ Osaka | Method for culturing pluripotent stem cells |
-
2015
- 2015-06-29 CA CA2953670A patent/CA2953670A1/en not_active Abandoned
- 2015-06-29 KR KR1020177000761A patent/KR20170020431A/en unknown
- 2015-06-29 US US15/321,835 patent/US11230701B2/en active Active
- 2015-06-29 EP EP15811956.0A patent/EP3162812A4/en active Pending
- 2015-06-29 AU AU2015281030A patent/AU2015281030A1/en not_active Abandoned
- 2015-06-29 JP JP2016529687A patent/JP6355736B2/en active Active
- 2015-06-29 CN CN201580034073.5A patent/CN106459223A/en active Pending
- 2015-06-29 BR BR112016030510A patent/BR112016030510A2/en not_active Application Discontinuation
- 2015-06-29 WO PCT/JP2015/068715 patent/WO2015199243A1/en active Application Filing
- 2015-06-29 SG SG11201610878QA patent/SG11201610878QA/en unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110053244A1 (en) * | 2009-08-27 | 2011-03-03 | Oyler George A | NOVEL PROTEIN DELIVERY SYSTEM TO GENERATE INDUCED PLURIPOTENT STEM (iPS) CELLS OR TISSUE-SPECIFIC CELLS |
| WO2013013105A2 (en) * | 2011-07-19 | 2013-01-24 | Vivoscript,Inc. | Compositions and methods for re-programming cells without genetic modification for repairing cartilage damage |
Non-Patent Citations (3)
| Title |
|---|
| K. LEE ET AL: "Molecular basis for disruption of E-cadherin adhesion by botulinum neurotoxin A complex", SCIENCE, vol. 344, no. 6190, 19 June 2014 (2014-06-19), pages 1405 - 1410, XP055415705, ISSN: 0036-8075, DOI: 10.1126/science.1253823 * |
| KWANGKOOK LEE ET AL: "Structure of a Bimodular Botulinum Neurotoxin Complex Provides Insights into Its Oral Toxicity", PLOS PATHOGENS, vol. 9, no. 10, 10 October 2013 (2013-10-10), pages e1003690, XP055415963, DOI: 10.1371/journal.ppat.1003690 * |
| See also references of WO2015199243A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| US20170130206A1 (en) | 2017-05-11 |
| EP3162812A1 (en) | 2017-05-03 |
| CN106459223A (en) | 2017-02-22 |
| WO2015199243A1 (en) | 2015-12-30 |
| US11230701B2 (en) | 2022-01-25 |
| BR112016030510A2 (en) | 2017-10-31 |
| KR20170020431A (en) | 2017-02-22 |
| CA2953670A1 (en) | 2015-12-30 |
| SG11201610878QA (en) | 2017-02-27 |
| AU2015281030A1 (en) | 2017-02-16 |
| JP6355736B2 (en) | 2018-07-11 |
| JPWO2015199243A1 (en) | 2017-04-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
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| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
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| STAA | Information on the status of an ep patent application or granted ep patent |
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| 17P | Request for examination filed |
Effective date: 20170119 |
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| AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
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| AX | Request for extension of the european patent |
Extension state: BA ME |
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| DAV | Request for validation of the european patent (deleted) | ||
| DAX | Request for extension of the european patent (deleted) | ||
| RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12N 5/10 20060101ALI20171018BHEP Ipc: C12N 1/00 20060101ALI20171018BHEP Ipc: C12N 5/0735 20100101ALI20171018BHEP Ipc: C07K 19/00 20060101AFI20171018BHEP Ipc: C07K 14/33 20060101ALI20171018BHEP Ipc: C12N 15/09 20060101ALI20171018BHEP |
|
| A4 | Supplementary search report drawn up and despatched |
Effective date: 20180125 |
|
| RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12N 5/0735 20100101ALI20180119BHEP Ipc: C12N 15/09 20060101ALI20180119BHEP Ipc: C07K 19/00 20060101AFI20180119BHEP Ipc: C12N 5/10 20060101ALI20180119BHEP Ipc: C07K 14/33 20060101ALI20180119BHEP Ipc: C12N 1/00 20060101ALI20180119BHEP |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: EXAMINATION IS IN PROGRESS |
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| 17Q | First examination report despatched |
Effective date: 20200421 |
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| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: EXAMINATION IS IN PROGRESS |