CA2928639A1 - Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells - Google Patents

Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells Download PDF

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Publication number
CA2928639A1
CA2928639A1 CA2928639A CA2928639A CA2928639A1 CA 2928639 A1 CA2928639 A1 CA 2928639A1 CA 2928639 A CA2928639 A CA 2928639A CA 2928639 A CA2928639 A CA 2928639A CA 2928639 A1 CA2928639 A1 CA 2928639A1
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Prior art keywords
cells
cell
differentiation
stage
pluripotent
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Abandoned
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CA2928639A
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English (en)
French (fr)
Inventor
Benjamin Fryer
Daina LANIAUSKAS
Marcia BLACKMOORE
Haiyun Wang
Kostadinka LILOVA
Shelley Nelson
Elizabeth ROSOCHA
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Janssen Biotech Inc
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Janssen Biotech Inc
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Priority claimed from US13/998,974 external-priority patent/US10377989B2/en
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of CA2928639A1 publication Critical patent/CA2928639A1/en
Abandoned legal-status Critical Current

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    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0676Pancreatic cells
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    • A61P1/00Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
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CA2928639A 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells Abandoned CA2928639A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361962158P 2013-11-01 2013-11-01
US61/962,158 2013-11-01
US13/998,974 2013-12-30
US13/998,974 US10377989B2 (en) 2012-12-31 2013-12-30 Methods for suspension cultures of human pluripotent stem cells
PCT/US2014/038993 WO2015065524A2 (en) 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

Publications (1)

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CA2928639A1 true CA2928639A1 (en) 2015-05-07

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CA2928639A Abandoned CA2928639A1 (en) 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells
CA2928741A Abandoned CA2928741A1 (en) 2013-11-01 2014-06-17 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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CA2928741A Abandoned CA2928741A1 (en) 2013-11-01 2014-06-17 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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EP (2) EP3063268A4 (enExample)
JP (2) JP2016534731A (enExample)
KR (4) KR20180128529A (enExample)
CN (2) CN105793413A (enExample)
AU (4) AU2014342995C1 (enExample)
BR (1) BR112016009393A8 (enExample)
CA (2) CA2928639A1 (enExample)
MX (2) MX2016005657A (enExample)
PH (2) PH12016500783A1 (enExample)
RU (2) RU2689710C2 (enExample)
SG (2) SG11201603045VA (enExample)
WO (2) WO2015065524A2 (enExample)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015065524A2 (en) * 2013-11-01 2015-05-07 Janssen Biotech, Inc. Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells
AU2017281631B2 (en) * 2016-06-23 2023-06-15 Tithon Biotech, Inc. Cells expressing parathyroid hormone 1 receptor and uses thereof
US11623023B2 (en) 2016-11-10 2023-04-11 Viacyte, Inc. PDX1 pancreatic endoderm cells in cell delivery devices and methods thereof
CA3042562C (en) * 2016-11-16 2023-02-28 Cynata Therapeutics Limited Pluripotent stem cell assay
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
EP4488363A3 (en) * 2017-07-21 2025-03-26 Vertex Pharmaceuticals Incorporated Re-aggregation of stem cell-derived pancreatic beta cells
JP7000896B2 (ja) * 2017-08-23 2022-01-19 王子ホールディングス株式会社 細胞シート形成部材、細胞シート形成部材の製造方法、および、細胞シートの製造方法
CA3087344A1 (en) * 2018-01-05 2019-07-11 Platelet Biogenesis, Inc. Compositions and methods for producing megakaryocytes
CN112041428B (zh) 2018-01-18 2024-05-24 新加坡科技研究局 用于在悬浮培养物中分化人多能干细胞系的方法
KR102115360B1 (ko) * 2018-05-30 2020-05-26 주식회사 바이블리오테카 성체 줄기세포 배양액 및 그 제조방법
US10724052B2 (en) 2018-09-07 2020-07-28 Crispr Therapeutics Ag Universal donor cells
US20220234006A1 (en) 2019-05-31 2022-07-28 W. L. Gore & Associates, Inc. A biocompatible membrane composite
CA3139585C (en) 2019-05-31 2024-01-23 W. L. Gore & Associates, Inc. A biocompatible membrane composite
CN114401752B (zh) 2019-05-31 2023-04-04 W.L.戈尔及同仁股份有限公司 具有受控氧扩散距离的细胞封装装置
JP2022534545A (ja) 2019-05-31 2022-08-01 ダブリュ.エル.ゴア アンド アソシエイツ,インコーポレイティド 生体適合性メンブレン複合体
AU2020337921A1 (en) * 2019-08-27 2022-03-17 United Therapeutics Corporation Methods and compositions for culturing alveolar cells
US11116797B2 (en) 2019-09-05 2021-09-14 Crispr Therapeutics Ag Universal donor cells
US11118196B2 (en) 2019-09-05 2021-09-14 Crispr Therapeutics Ag Universal donor cells
US20220389378A1 (en) * 2019-11-22 2022-12-08 Novo Nordisk A/S Spin-aggregated neural microspheres and the application thereof
CA3203707A1 (en) 2020-12-31 2022-07-07 Alireza Rezania Universal donor cells
JPWO2022203051A1 (enExample) * 2021-03-25 2022-09-29
CN116218766A (zh) * 2023-04-21 2023-06-06 北京意胜生物科技有限公司 一种制备胰腺前体细胞的方法、培养基及其应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ2004696A3 (cs) * 2001-11-09 2005-02-16 Artecel Sciences, Inc. Endokrinní pankreatická diferenciace buněk stromatu odvozených z adipózní tkáně a její použití
EP2059586B1 (en) * 2006-08-02 2016-07-20 Technion Research & Development Foundation Ltd. Methods of expanding embryonic stem cells in a suspension culture
US20110151554A1 (en) * 2006-11-09 2011-06-23 Akira Yuo Method for culturing and subculturing primate embryonic stem cell, as well as method for inducing differentiation thereof
WO2008148105A1 (en) * 2007-05-25 2008-12-04 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
CN101978044B (zh) * 2007-07-01 2017-10-24 生命扫描有限公司 单个多能干细胞培养
EP2479260B1 (en) * 2008-03-17 2016-01-06 Agency For Science, Technology And Research Microcarriers for stem cell culture
BRPI0914116A2 (pt) * 2008-06-30 2019-09-17 Centocor Ortho Biotech Inc diferenciação de células-tronco pluripotentes
US8008075B2 (en) * 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
CA2770753C (en) * 2009-08-12 2019-01-15 Kyoto University Method for inducing differentiation of pluripotent stem cells into neural precursor cells
WO2011158960A1 (en) * 2010-06-15 2011-12-22 Kyoto University Method for selecting human induced pluripotent stem cells
US9528090B2 (en) * 2010-08-31 2016-12-27 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
WO2012117333A1 (en) * 2011-02-28 2012-09-07 Stempeutics Research Malaysia Sdn Bhd Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof
US8951798B2 (en) * 2011-10-13 2015-02-10 Wisconsin Alumni Research Foundation Generation of cardiomyocytes from human pluripotent stem cells
SG10201608914WA (en) * 2011-12-22 2016-12-29 Janssen Biotech Inc Differentiation of human embryonic stem cells into single hormonal insulin positive cells
US10519422B2 (en) * 2012-02-29 2019-12-31 Riken Method of producing human retinal pigment epithelial cells
KR20150103203A (ko) * 2012-12-31 2015-09-09 얀센 바이오테크 인코포레이티드 췌장 내분비 세포 내로의 분화를 위한 인간 만능 세포의 현탁 및 클러스터링
WO2015065524A2 (en) * 2013-11-01 2015-05-07 Janssen Biotech, Inc. Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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