JP2016529904A - 効率的な非減数分裂対立遺伝子移入 - Google Patents

効率的な非減数分裂対立遺伝子移入 Download PDF

Info

Publication number
JP2016529904A
JP2016529904A JP2016538910A JP2016538910A JP2016529904A JP 2016529904 A JP2016529904 A JP 2016529904A JP 2016538910 A JP2016538910 A JP 2016538910A JP 2016538910 A JP2016538910 A JP 2016538910A JP 2016529904 A JP2016529904 A JP 2016529904A
Authority
JP
Japan
Prior art keywords
sequence
allele
dna
talen
hdr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2016538910A
Other languages
English (en)
Japanese (ja)
Other versions
JP2016529904A5 (enExample
Inventor
ダニエル・エフ・カールソン
スコット・シー・ファーレンクラッグ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Recombinetics Inc
Original Assignee
Recombinetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Recombinetics Inc filed Critical Recombinetics Inc
Publication of JP2016529904A publication Critical patent/JP2016529904A/ja
Publication of JP2016529904A5 publication Critical patent/JP2016529904A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8778—Swine embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2016538910A 2013-08-27 2014-04-29 効率的な非減数分裂対立遺伝子移入 Pending JP2016529904A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361870401P 2013-08-27 2013-08-27
US61/870,401 2013-08-27
PCT/US2014/035854 WO2015030881A1 (en) 2013-08-27 2014-04-29 Efficient non-meiotic allele introgression

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020023018A Division JP2020089386A (ja) 2013-08-27 2020-02-14 効率的な非減数分裂対立遺伝子移入

Publications (2)

Publication Number Publication Date
JP2016529904A true JP2016529904A (ja) 2016-09-29
JP2016529904A5 JP2016529904A5 (enExample) 2017-06-15

Family

ID=52585270

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2016538910A Pending JP2016529904A (ja) 2013-08-27 2014-04-29 効率的な非減数分裂対立遺伝子移入
JP2020023018A Pending JP2020089386A (ja) 2013-08-27 2020-02-14 効率的な非減数分裂対立遺伝子移入

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2020023018A Pending JP2020089386A (ja) 2013-08-27 2020-02-14 効率的な非減数分裂対立遺伝子移入

Country Status (14)

Country Link
US (6) US9528124B2 (enExample)
EP (1) EP3039145B1 (enExample)
JP (2) JP2016529904A (enExample)
KR (1) KR20160048179A (enExample)
CN (2) CN111471719B (enExample)
AP (1) AP2016009113A0 (enExample)
AR (1) AR096143A1 (enExample)
AU (2) AU2014311855A1 (enExample)
BR (1) BR112016004020B1 (enExample)
CA (1) CA2922084C (enExample)
MX (1) MX2016002564A (enExample)
PH (1) PH12016500378A1 (enExample)
RU (1) RU2016110827A (enExample)
WO (1) WO2015030881A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023525513A (ja) * 2020-05-06 2023-06-16 セレクティス ソシエテ アノニム 細胞ゲノムにおける外因性配列の標的化挿入のための方法
WO2026034472A1 (ja) * 2024-08-06 2026-02-12 武田薬品工業株式会社 外因性核酸が組み込まれた細胞の製造方法

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10920242B2 (en) * 2011-02-25 2021-02-16 Recombinetics, Inc. Non-meiotic allele introgression
US9528124B2 (en) * 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
ES2962492T3 (es) 2013-10-25 2024-03-19 Livestock Improvement Corporation Ltd Marcadores genéticos y usos de los mismos
CN111218447B (zh) 2013-11-07 2024-10-11 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
MX388127B (es) 2013-12-11 2025-03-19 Regeneron Pharma Metodos y composiciones para la modificacion dirigida de un genoma.
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
AU2015236033B2 (en) * 2014-03-26 2018-06-07 University Of Maryland, College Park Targeted genome editing in zygotes of domestic large animals
BR122023023211A2 (pt) 2014-04-28 2024-01-23 Recombinetics, Inc. Método de fazer edições de genes multiplex em uma célula de vertebrado ou de embrião primário não-humano
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
AU2015320738B2 (en) 2014-09-23 2021-12-02 Acceligen, Inc. Materials and methods for producing animals with short hair
EP3204496A1 (en) 2014-10-10 2017-08-16 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
WO2016073990A2 (en) 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing
CA2969619A1 (en) 2014-12-03 2016-06-09 Agilent Technologies, Inc. Guide rna with chemical modifications
CN107787367B (zh) 2015-04-06 2021-10-26 里兰斯坦福初级大学理事会 用于crispr/cas介导的基因调控的化学修饰的引导rna
CN108368502B (zh) 2015-06-03 2022-03-18 内布拉斯加大学董事委员会 使用单链dna的dna编辑
EP4339287A3 (en) 2015-07-31 2024-05-22 Regents Of The University Of Minnesota Modified cells and methods of therapy
WO2017024343A1 (en) * 2015-08-07 2017-02-16 Commonwealth Scientific And Industrial Research Organisation Method for producing an animal comprising a germline genetic modification
AU2016315942A1 (en) * 2015-09-01 2018-04-19 Recombinetics, Inc. Method of identifying the presence of foreign alleles in a desired haplotype
EP3352567A4 (en) * 2015-09-21 2019-02-27 Recombinetics, Inc. GENETICALLY MODIFIED ANIMALS WITH INCREASED HEAT TOLERANCE
CA2999500A1 (en) 2015-09-24 2017-03-30 Editas Medicine, Inc. Use of exonucleases to improve crispr/cas-mediated genome editing
GB201517227D0 (en) * 2015-09-29 2015-11-11 Univ Edinburgh Genetically-edited swine
WO2017070632A2 (en) 2015-10-23 2017-04-27 President And Fellows Of Harvard College Nucleobase editors and uses thereof
EP3383409A4 (en) * 2015-12-02 2019-10-02 The Regents of The University of California COMPOSITIONS AND METHOD FOR MODIFYING A TARGET NUCLEIC ACID
CA3303511A1 (en) * 2016-01-26 2026-05-13 Cedars-Sinai Medical Center SYSTEMS AND METHODS FOR IN VIVO DUAL RECOMBINASE-MEDIATED CASSETTE EXCHANGE (dRMCE) AND DISEASE MODELS THEREOF
US11274288B2 (en) 2016-02-16 2022-03-15 Emendobio Inc. Compositions and methods for promoting homology directed repair mediated gene editing
WO2017165826A1 (en) 2016-03-25 2017-09-28 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
US11236313B2 (en) 2016-04-13 2022-02-01 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
WO2018027078A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof
AU2017308889B2 (en) 2016-08-09 2023-11-09 President And Fellows Of Harvard College Programmable Cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR20240007715A (ko) 2016-10-14 2024-01-16 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
EP4338799A3 (en) 2016-10-18 2024-06-05 Regents of the University of Minnesota Tumor infiltrating lymphocytes and methods of therapy
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
KR20190123328A (ko) 2017-03-09 2019-10-31 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 암 백신
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
CN110914310A (zh) 2017-03-10 2020-03-24 哈佛大学的校长及成员们 胞嘧啶至鸟嘌呤碱基编辑器
IL306092A (en) 2017-03-23 2023-11-01 Harvard College Nucleic base editors that include nucleic acid programmable DNA binding proteins
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
CN111511375A (zh) 2017-06-30 2020-08-07 因提玛生物科学公司 用于基因治疗的腺相关病毒载体
EP3652312A1 (en) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
EP3658573A1 (en) 2017-07-28 2020-06-03 President and Fellows of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
CA3082251A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
CN112654710A (zh) 2018-05-16 2021-04-13 辛瑟高公司 用于指导rna设计和使用的方法和系统
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
EP3820495A4 (en) 2018-07-09 2022-07-20 The Broad Institute Inc. RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
WO2020191241A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
CN110211635B (zh) * 2019-06-12 2020-07-21 北京康普森农业科技有限公司 用于畜禽基因组选择分析的方法及畜禽育种方法
CA3140297A1 (en) * 2019-06-19 2020-12-24 Simon Auslaender Method for the generation of a protein expressing cell by targeted integration using cre mrna
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
CN112889744B (zh) * 2019-12-04 2022-11-11 广西贵港秀博基因科技股份有限公司 育种方法和育种系统
CA3177481A1 (en) 2020-05-08 2021-11-11 David R. Liu Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
CN112725468B (zh) * 2021-03-04 2022-06-07 华南农业大学 肉鸡腹脂率分子标记APOB c.246及检测方法
CN112941108B (zh) * 2021-05-13 2021-10-15 中国农业科学院北京畜牧兽医研究所 一种具有无角Pc位点纯合基因型的荷斯坦牛的制备方法
CN113416780A (zh) * 2021-07-30 2021-09-21 丽水市中心医院 一种诊治缺血性脑卒中的分子标志物
WO2023039586A1 (en) 2021-09-10 2023-03-16 Agilent Technologies, Inc. Guide rnas with chemical modification for prime editing
CN114262708B (zh) * 2021-12-21 2024-07-02 西北农林科技大学 一种生产FecB基因g.A746G定点突变绵羊的试剂盒及其方法
CN114752686B (zh) * 2022-04-25 2023-05-16 四川省畜牧科学研究院 一种与兔肌肉品质性状相关的snp分子标记及其应用
KR102697859B1 (ko) 2023-09-14 2024-08-23 (주)오토노머스에이투지 카메라에 오염이 발생했는지 여부를 실시간으로 판단하는 방법 및 이를 이용한 컴퓨팅 장치

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130117870A1 (en) * 2011-02-25 2013-05-09 Scott C. Fahrenkrug Genetically modified animals and methods for making the same
JP2013518586A (ja) * 2010-02-08 2013-05-23 サンガモ バイオサイエンシーズ， インコーポレイテッド 遺伝子操作された切断ハーフドメイン
JP2013529083A (ja) * 2010-05-17 2013-07-18 サンガモ バイオサイエンシーズ， インコーポレイテッド 新規のｄｎａ結合タンパク質及びその使用

Family Cites Families (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
US5185444A (en) 1985-03-15 1993-02-09 Anti-Gene Deveopment Group Uncharged morpolino-based polymers having phosphorous containing chiral intersubunit linkages
JP2528107B2 (ja) 1985-03-15 1996-08-28 サマ−トン，ジエ−ムス ポリヌクレオチド測定試薬と方法
US5731178A (en) 1990-03-21 1998-03-24 Behringwerke Aktiengesellschaft Attachment-elements for stimulation of eukaryotic expression systems
US5763240A (en) 1992-04-24 1998-06-09 Sri International In vivo homologous sequence targeting in eukaryotic cells
FR2737497B1 (fr) 1995-08-02 1997-10-03 Agronomique Inst Nat Rech Utilisation du domaine intra-cytoplasmique du recepteur de la prolactine ou du recepteur de l'hormone de croissance pour obtenir la secretion de proteines
EP0821070A1 (en) 1996-07-22 1998-01-28 Carelli, Claude Marcel Henri Pit-1 gene polymorphism and trait selection in animals
US5830698A (en) 1997-03-14 1998-11-03 Idec Pharmaceuticals Corporation Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
WO1999002667A1 (en) 1997-07-14 1999-01-21 University Of Liege Mutations in the myostation gene cause double-muscling in mammals
WO2000011155A1 (en) 1998-08-19 2000-03-02 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for genomic modification
AU6218899A (en) 1998-10-12 2000-05-01 Geron Bio-Med Limited Porcine oocytes with improved developmental competence
WO2001030965A2 (en) 1999-10-28 2001-05-03 The Board Of Trustees Of The Leland Stanford Junior University Methods of in vivo gene transfer using a sleeping beauty transposon system
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
WO2001061049A1 (en) 2000-02-18 2001-08-23 The Board Of Trustees Of The Leland Stanford Junior University Altered recombinases for genome modification
CN101024829A (zh) 2000-02-24 2007-08-29 马萨诸塞大学 只生育单一性别后代的哺乳动物的生产
US6534299B2 (en) 2000-05-17 2003-03-18 Applera Corporation Isolated human kinase proteins, nucleic acid molecules encoding human kinase proteins, and uses thereof
IL154751A0 (en) 2000-08-03 2003-10-31 Humanized antibodies, recombination vectors, transgenic vectors and methods for the preparation of humanized antibodies from transgenic animals
ES2394877T3 (es) 2000-08-14 2013-02-06 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Recombinación homóloga mejorada mediada por proteínas de recombinación de lambda
US7645441B2 (en) 2000-12-22 2010-01-12 Sagres Discovery Inc. Compositions and methods in cancer associated with altered expression of PRLR
US7199281B2 (en) 2001-09-07 2007-04-03 The Regents Of The University Of California Method of generating a transgenic livestock animal
EP1325930A1 (en) 2002-01-08 2003-07-09 Institut National De La Sante Et De La Recherche Medicale (Inserm) Mammal prolactin variants
ATE347593T1 (de) 2002-01-23 2006-12-15 Univ Utah Res Found Zielgerichtete chromosomale mutagenese mit zinkfingernukleasen
EP1504092B2 (en) 2002-03-21 2014-06-25 Sangamo BioSciences, Inc. Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
EP1563084A4 (en) 2002-11-08 2006-03-01 Hematech Llc TRANSGENE UNGULATES WITH REDUCED PRION PROTEIN ACTIVITY AND USES THEREOF
CA2506798C (en) 2002-11-22 2013-01-22 Institut Clayton De La Recherche Compositions and systems for the regulation of genes
US7176007B2 (en) 2002-12-12 2007-02-13 Wisconsin Alumni Research Foundation RecA mutants
CA2512110A1 (en) 2002-12-31 2004-07-22 Mmi Genomics, Inc. Compositions, methods, and systems for inferring bovine breed
WO2004065581A2 (en) 2003-01-15 2004-08-05 Discovery Genomics, Inc. Transposon-insulator element delivery systems
US7985739B2 (en) 2003-06-04 2011-07-26 The Board Of Trustees Of The Leland Stanford Junior University Enhanced sleeping beauty transposon system and methods for using the same
US20120196370A1 (en) 2010-12-03 2012-08-02 Fyodor Urnov Methods and compositions for targeted genomic deletion
BRPI0415468A (pt) 2003-10-24 2007-03-27 Mmi Genomics Inc métodos e sistemas para inferir caracterìsticas para controlar criação não de gado
US7972783B2 (en) 2003-11-24 2011-07-05 Branhaven LLC Method and markers for determining the genotype of horned/polled cattle
TW200724548A (en) 2005-04-25 2007-07-01 Pfizer Antibodies to myostatin
AU2006262020B8 (en) 2005-06-24 2011-02-17 Recombinetics, Inc. Using cytosine deaminases to diminish retroelement transfer from pigs to humans
WO2008074101A2 (en) 2006-12-21 2008-06-26 Agriculture Victoria Services Pty Limited Artificial selection method and reagents
CA2716990A1 (en) 2007-03-07 2008-09-12 Aarhus Universitet Pig model for atherosclerosis
US20110262909A1 (en) 2007-10-03 2011-10-27 Pfizer Genetic Markers for Horned and Polled Cattle and Related Methods
WO2010008562A2 (en) 2008-07-16 2010-01-21 Recombinetics Methods and materials for producing transgenic animals
WO2010008564A2 (en) 2008-07-16 2010-01-21 Recombinetics Plaice dna transposon system
US20110023140A1 (en) 2008-12-04 2011-01-27 Sigma-Aldrich Co. Rabbit genome editing with zinc finger nucleases
US20110023159A1 (en) 2008-12-04 2011-01-27 Sigma-Aldrich Co. Ovine genome editing with zinc finger nucleases
US20120030778A1 (en) 2008-12-04 2012-02-02 Sigma-Aldrich Co., Llc. Genomic editing of genes involved with parkinsons disease
US20110023158A1 (en) 2008-12-04 2011-01-27 Sigma-Aldrich Co. Bovine genome editing with zinc finger nucleases
KR20100080068A (ko) 2008-12-31 2010-07-08 주식회사 툴젠 신규한 징크 핑거 뉴클레아제 및 이의 용도
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
KR20100133319A (ko) 2009-06-11 2010-12-21 주식회사 툴젠 표적 특이적인 게놈의 재배열을 위한 표적 특이적 뉴클레아제 및 이의 용도
EP2449112A1 (en) 2009-07-01 2012-05-09 Transposagen Biopharmaceuticals, Inc. Genetically modified rat models for severe combined immunodeficiency (scid)
KR20120097483A (ko) 2009-07-24 2012-09-04 시그마-알드리치 컴퍼니., 엘엘씨 게놈 편집을 위한 방법
US20120192298A1 (en) 2009-07-24 2012-07-26 Sigma Aldrich Co. Llc Method for genome editing
NZ598457A (en) 2009-08-03 2014-06-27 Recombinetics Inc Methods and compositions for targeted gene modification
CN102612565B (zh) 2009-08-11 2016-04-06 桑格摩生物科学股份有限公司 靶向修饰的纯合生物体
PT2816112T (pt) 2009-12-10 2018-11-20 Univ Iowa State Res Found Inc Modificação do adn modificada pelo efector tal
US20130023051A1 (en) 2009-12-21 2013-01-24 Keygene N.V. Techniques for transfecting protoplasts
US9255259B2 (en) 2010-02-09 2016-02-09 Sangamo Biosciences, Inc. Targeted genomic modification with partially single-stranded donor molecules
US20110197290A1 (en) 2010-02-11 2011-08-11 Fahrenkrug Scott C Methods and materials for producing transgenic artiodactyls
EP2392208B1 (en) 2010-06-07 2016-05-04 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Fusion proteins comprising a DNA-binding domain of a Tal effector protein and a non-specific cleavage domain of a restriction nuclease and their use
WO2011159369A1 (en) 2010-06-14 2011-12-22 Iowa State University Research Foundation, Inc. Nuclease activity of tal effector and foki fusion protein
WO2012012738A1 (en) 2010-07-23 2012-01-26 Sigma-Aldrich Co., Llc Genome editing using targeting endonucleases and single-stranded nucleic acids
GB201014169D0 (en) 2010-08-25 2010-10-06 Cambridge Entpr Ltd In vitro hepatic differentiation
WO2012093833A2 (en) 2011-01-03 2012-07-12 Toolgen Incorporation Genome engineering via designed tal effector nucleases
WO2012094132A1 (en) 2011-01-05 2012-07-12 Sangamo Biosciences, Inc. Methods and compositions for gene correction
US20140201857A1 (en) * 2013-01-14 2014-07-17 Recombinetics, Inc. Hornless livestock
US10920242B2 (en) 2011-02-25 2021-02-16 Recombinetics, Inc. Non-meiotic allele introgression
US9528124B2 (en) * 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
WO2012152912A1 (en) 2011-05-12 2012-11-15 Newvectys Genetically modified pig as a cancer prone model
PT3434775T (pt) 2011-06-07 2020-04-08 Helmholtz Zentrum Muenchen Deutsches Forschungszentrum Gesundheit & Umwelt Gmbh Proteína que tem atividade de nuclease, proteínas de fusão e utilizações das mesmas
WO2012168307A2 (en) 2011-06-07 2012-12-13 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Improved recombination efficiency by inhibition of nhej dna repair
CN104080462B (zh) 2011-12-16 2019-08-09 塔尔盖特基因生物技术有限公司 用于修饰预定的靶核酸序列的组合物和方法
US20130212722A1 (en) 2012-02-15 2013-08-15 Mice With Horns, Llc Transgenic animals with customizable traits
CA2871524C (en) 2012-05-07 2021-07-27 Sangamo Biosciences, Inc. Methods and compositions for nuclease-mediated targeted integration of transgenes
AR091482A1 (es) * 2012-06-21 2015-02-04 Recombinetics Inc Celulas modificadas geneticamente y metodos par su obtencion
WO2013191769A1 (en) 2012-06-22 2013-12-27 Mayo Foundation For Medical Education And Research Genome editing
US10058078B2 (en) 2012-07-31 2018-08-28 Recombinetics, Inc. Production of FMDV-resistant livestock by allele substitution
GB201216564D0 (en) 2012-09-17 2012-10-31 Univ Edinburgh Genetically edited animal
KR102955556B1 (ko) 2012-12-12 2026-04-22 더 브로드 인스티튜트, 인코퍼레이티드 서열 조작을 위한 crispr-cas 성분 시스템, 방법 및 조성물
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
AU2014235968B2 (en) * 2013-03-21 2018-05-24 Ospedale San Raffaele Srl Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases
EP4286517A3 (en) * 2013-04-04 2024-03-13 President and Fellows of Harvard College Therapeutic uses of genome editing with crispr/cas systems
US20150013025A1 (en) 2013-05-19 2015-01-08 Mice With Horns, Llc Transgenic animals with customizable traits
ES2962492T3 (es) 2013-10-25 2024-03-19 Livestock Improvement Corporation Ltd Marcadores genéticos y usos de los mismos
BR122023023211A2 (pt) 2014-04-28 2024-01-23 Recombinetics, Inc. Método de fazer edições de genes multiplex em uma célula de vertebrado ou de embrião primário não-humano
AU2015320738B2 (en) 2014-09-23 2021-12-02 Acceligen, Inc. Materials and methods for producing animals with short hair
EP3352567A4 (en) 2015-09-21 2019-02-27 Recombinetics, Inc. GENETICALLY MODIFIED ANIMALS WITH INCREASED HEAT TOLERANCE
US20170099813A1 (en) * 2015-10-07 2017-04-13 Recombinetics, Inc. Method of generating sterile terminal sires in livestock and animals produced thereby

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013518586A (ja) * 2010-02-08 2013-05-23 サンガモ バイオサイエンシーズ， インコーポレイテッド 遺伝子操作された切断ハーフドメイン
JP2013529083A (ja) * 2010-05-17 2013-07-18 サンガモ バイオサイエンシーズ， インコーポレイテッド 新規のｄｎａ結合タンパク質及びその使用
US20130117870A1 (en) * 2011-02-25 2013-05-09 Scott C. Fahrenkrug Genetically modified animals and methods for making the same

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ACS SYNTH. BIOL., 2013.08.26, VOL.2, P.604-613, JPN6018011680, ISSN: 0003976474 *
CELL, 2013.05.09, VOL.153, P.910-918, JPN6019004725, ISSN: 0004134353 *
J. GENET. GENOMICS, AVAILABLE ONLINE 2013.04.12, VOL.40, P.281-289, JPN6018011677, ISSN: 0004134354 *
NATURE, 2012, VOL.491, P.114-118, JPN6018011683, ISSN: 0003976475 *
SCIENCE, 2013.02.15, VOL.339, P.819-823, JPN6019004730, ISSN: 0003976476 *
SCIENCE, 2013.02.15, VOL.339, P.823-826, JPN6019004732, ISSN: 0003976477 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023525513A (ja) * 2020-05-06 2023-06-16 セレクティス ソシエテ アノニム 細胞ゲノムにおける外因性配列の標的化挿入のための方法
JP7750865B2 (ja) 2020-05-06 2025-10-07 セレクティス ソシエテ アノニム 細胞ゲノムにおける外因性配列の標的化挿入のための方法
WO2026034472A1 (ja) * 2024-08-06 2026-02-12 武田薬品工業株式会社 外因性核酸が組み込まれた細胞の製造方法

Also Published As

Publication number Publication date
US20190106708A1 (en) 2019-04-11
RU2016110827A (ru) 2017-10-03
AU2014311855A1 (en) 2016-04-14
US20150067898A1 (en) 2015-03-05
PH12016500378A1 (en) 2016-05-02
US9528124B2 (en) 2016-12-27
CN111471719A (zh) 2020-07-31
AP2016009113A0 (en) 2016-03-31
EP3039145B1 (en) 2019-12-18
RU2016110827A3 (enExample) 2018-03-19
US20150156996A1 (en) 2015-06-11
US20180051298A1 (en) 2018-02-22
US10959414B2 (en) 2021-03-30
US11477969B2 (en) 2022-10-25
CN111471719B (zh) 2024-06-18
WO2015030881A1 (en) 2015-03-05
NZ718194A (en) 2021-10-29
CN105960461B (zh) 2020-03-17
CA2922084A1 (en) 2015-03-05
US20190194687A1 (en) 2019-06-27
KR20160048179A (ko) 2016-05-03
MX2016002564A (es) 2016-10-13
EP3039145A1 (en) 2016-07-06
AU2020286315B2 (en) 2022-02-17
BR112016004020A2 (pt) 2017-12-05
CA2922084C (en) 2023-08-29
AR096143A1 (es) 2015-12-09
CN105960461A (zh) 2016-09-21
US20240057570A1 (en) 2024-02-22
BR112016004020B1 (pt) 2022-08-30
JP2020089386A (ja) 2020-06-11
AU2020286315A1 (en) 2021-01-21
EP3039145A4 (en) 2017-06-07

Similar Documents

Publication Publication Date Title
US20240057570A1 (en) Efficient non-meiotic allele introgression in livestock
JP6815986B2 (ja) 大型家畜の接合体における標的化ゲノム編集
US20140201857A1 (en) Hornless livestock
US20190223417A1 (en) Genetically modified animals having increased heat tolerance
US10893667B2 (en) Non-meiotic allele introgression
JP2018531003A6 (ja) 向上した耐暑性を有する遺伝子改変動物
JP2017533716A (ja) 腫瘍抑制因子遺伝子のヘテロ接合性改変および神経線維腫症１型のブタモデル
US20240397918A1 (en) Non-meiotic allele introgression
NZ718194B2 (en) Efficient non-meiotic allele introgression
HK1229365A1 (en) Efficient non-meiotic allele introgression

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170427

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20170427

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20180403

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20180702

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20180831

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180928

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20190219

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190515

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20191015