JP2016519577A5 - - Google Patents
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- JP2016519577A5 JP2016519577A5 JP2016507665A JP2016507665A JP2016519577A5 JP 2016519577 A5 JP2016519577 A5 JP 2016519577A5 JP 2016507665 A JP2016507665 A JP 2016507665A JP 2016507665 A JP2016507665 A JP 2016507665A JP 2016519577 A5 JP2016519577 A5 JP 2016519577A5
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- JP
- Japan
- Prior art keywords
- recombinant cell
- seq
- polypeptide
- recombinant
- phosphate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- WASNIKZYIWZQIP-AWEZNQCLSA-N nerolidol Natural products CC(=CCCC(=CCC[C@@H](O)C=C)C)C WASNIKZYIWZQIP-AWEZNQCLSA-N 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- 239000010452 phosphate Substances 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 229930001119 polyketide Natural products 0.000 claims 1
- 125000000830 polyketide group Chemical group 0.000 claims 1
- 229920001550 polyprenyl Polymers 0.000 claims 1
- 125000001185 polyprenyl group Polymers 0.000 claims 1
- 150000003097 polyterpenes Chemical class 0.000 claims 1
- 238000005215 recombination Methods 0.000 claims 1
- 230000006798 recombination Effects 0.000 claims 1
- 229930006696 sabinene Natural products 0.000 claims 1
- -1 sequterpenes Chemical class 0.000 claims 1
- 230000002194 synthesizing effect Effects 0.000 claims 1
- 150000003535 tetraterpenes Chemical class 0.000 claims 1
- 235000009657 tetraterpenes Nutrition 0.000 claims 1
- CRDAMVZIKSXKFV-UHFFFAOYSA-N trans-Farnesol Natural products CC(C)=CCCC(C)=CCCC(C)=CCO CRDAMVZIKSXKFV-UHFFFAOYSA-N 0.000 claims 1
- 150000003648 triterpenes Chemical class 0.000 claims 1
- WCTNXGFHEZQHDR-UHFFFAOYSA-N valencene Natural products C1CC(C)(C)C2(C)CC(C(=C)C)CCC2=C1 WCTNXGFHEZQHDR-UHFFFAOYSA-N 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
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Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8647642B2 (en) | 2008-09-18 | 2014-02-11 | Aviex Technologies, Llc | Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment |
| SG11201508186XA (en) | 2013-04-10 | 2015-11-27 | Danisco Us Inc | Phosphoketolases for improved production of acetyl coenzyme a-derived metabolites, isoprene, isoprenoid precursors, and isoprenoids |
| EP3741865B1 (en) * | 2014-09-18 | 2024-03-13 | Genomatica, Inc. | Non-natural microbial organisms with improved energetic efficiency |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
| BR112019019701A2 (pt) * | 2017-03-24 | 2020-07-14 | Danisco Us Inc. | redução de acetato e glicerol em levedura modificada que tem um caminho de produção de etanol exógeno |
| CN107841523A (zh) * | 2017-10-19 | 2018-03-27 | 浙江大学 | 利用群体感应分子诱导从桦褐孔菌中提取三萜类物质方法 |
| WO2019113387A1 (en) | 2017-12-07 | 2019-06-13 | Zymergen Inc. | Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation |
| JP2021506316A (ja) | 2017-12-21 | 2021-02-22 | ザイマージェン インコーポレイテッド | ネペタラクトールオキシドレダクターゼ、ネペタラクトールシンターゼ、及びネペタラクトンを産生する能力がある微生物 |
| CA3090055A1 (en) | 2018-03-01 | 2019-09-06 | Total Raffinage Chimie | Metabolic pathways with increased carbon yield |
| CN109929883A (zh) * | 2019-04-02 | 2019-06-25 | 烟台华康荣赞生物科技有限公司 | 重组酵母、构建方法和其在制备酪醇及衍生物中的应用 |
| WO2021063958A1 (en) * | 2019-09-30 | 2021-04-08 | Global Bioenergies | Improved means and methods for increasing the yield of acetyl-coa from glucose |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR669447A (fr) | 1929-02-09 | 1929-11-15 | Dispositif pour arrêter automatiquement un moteur d'automobile après une période de temps prédéterminée | |
| DE19629568C1 (de) | 1996-07-15 | 1998-01-08 | Fraunhofer Ges Forschung | Verfahren zur Herstellung von Isopren |
| US7172886B2 (en) | 2001-12-06 | 2007-02-06 | The Regents Of The University Of California | Biosynthesis of isopentenyl pyrophosphate |
| JPWO2003095651A1 (ja) | 2002-05-10 | 2005-09-15 | 協和醗酵工業株式会社 | メバロン酸の製造方法 |
| KR101148255B1 (ko) | 2002-10-04 | 2012-08-08 | 다니스코 유에스 인크. | 고수율을 갖는 1,3-프로판디올의 생물학적 제조 방법 |
| DE602005020469D1 (de) * | 2004-08-10 | 2010-05-20 | Ajinomoto Kk | Verwendung von phosphoketolase zur herstellung geeigneter metabolite |
| RU2004124226A (ru) | 2004-08-10 | 2006-01-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | Использование фосфокетолазы для продукции полезных метаболитов |
| WO2007140339A2 (en) | 2006-05-26 | 2007-12-06 | Amyris Biotechnologies, Inc. | Production of isoprenoids |
| JP4986547B2 (ja) | 2006-09-04 | 2012-07-25 | 株式会社Adeka | 新規なアセトアセチルCoA合成酵素、それをコードするDNA配列、当該酵素の製造方法および当該酵素を利用したメバロン酸の製造方法 |
| WO2009005704A1 (en) * | 2007-07-03 | 2009-01-08 | The Regents Of The University Of California | Methods of increasing isoprenoid or isoprenoid precursor production |
| CN105368764B (zh) | 2007-12-13 | 2019-03-12 | 丹尼斯科美国公司 | 用于生产异戊二烯的组合物和方法 |
| US20110045563A1 (en) | 2008-02-06 | 2011-02-24 | The Regents Of The University Of California | Short chain volatile isoprene hydrocarbon production using the mevalonic acid pathway in genetically engineered yeast and fungi |
| WO2009132220A2 (en) | 2008-04-23 | 2009-10-29 | Danisco Us Inc. | Isoprene synthase variants for improved microbial production of isoprene |
| US7632995B2 (en) | 2008-05-09 | 2009-12-15 | Monsanto Technology Llc | Plants and seeds of hybrid corn variety CH852179 |
| MX318543B (es) | 2008-07-02 | 2014-03-18 | Danisco Us Inc | Composiciones y metodos para producir isopreno libre de hidrocarburos de c5 bajo condicion de desacoplamiento y/o intervalos de operacion seguros. |
| HU3516U (en) | 2008-07-31 | 2008-11-28 | Zoltan Kuthi | Anti-theft protection of draining for fuel tanks |
| WO2010031062A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Increased isoprene production using the archaeal lower mevalonate pathway |
| CA2737082A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Increased isoprene production using mevalonate kinase and isoprene synthase |
| EP2340312A2 (en) | 2008-09-15 | 2011-07-06 | Danisco US Inc. | Conversion of prenyl derivatives to isoprene |
| CA2737223A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Reduction of carbon dioxide emission during isoprene production by fermentation |
| EP2344629B1 (en) | 2008-09-15 | 2018-01-24 | Danisco US Inc. | Systems using cell culture for production of isoprene |
| MY161071A (en) | 2008-12-30 | 2017-04-14 | Danisco Us Inc | Methods of producing isoprene and a co-product |
| US8324442B2 (en) | 2009-03-03 | 2012-12-04 | Amyris, Inc. | Microbial derived isoprene and methods for making the same |
| US20100297749A1 (en) | 2009-04-21 | 2010-11-25 | Sapphire Energy, Inc. | Methods and systems for biofuel production |
| AU2010238770A1 (en) | 2009-04-23 | 2011-11-03 | Danisco Us Inc. | Three-dimensional structure of isoprene synthase and its use thereof for generating variants |
| JP4760951B2 (ja) | 2009-05-08 | 2011-08-31 | トヨタ自動車株式会社 | ブタノール生産能を有する組換え微生物及びブタノールの製造方法 |
| TW201412988A (zh) | 2009-06-17 | 2014-04-01 | Danisco Us Inc | 使用dxp及mva途徑之改良之異戊二烯製造 |
| TWI434921B (zh) | 2009-06-17 | 2014-04-21 | Danisco Us Inc | 從生物異戊二烯組合物製造燃料成分之方法及系統 |
| US20120252054A1 (en) | 2009-09-15 | 2012-10-04 | Sapphire Energy Inc | System for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp. |
| US8569562B2 (en) | 2009-12-18 | 2013-10-29 | Danisco Us Inc. | Purification of isoprene from renewable resources |
| SG196782A1 (en) | 2009-12-23 | 2014-02-13 | Danisco Us Inc | Compositions and methods for the increased production of isoprene and other products with 6 - phosphogluconolactonase (pgl) |
| US8871488B2 (en) | 2010-06-18 | 2014-10-28 | Butamax Advanced Biofuels Llc | Recombinant host cells comprising phosphoketolases |
| CA2816306A1 (en) | 2010-10-27 | 2012-05-03 | Danisco Us Inc. | Isoprene synthase variants for improved production of isoprene |
| BR112013027555A2 (pt) * | 2011-04-29 | 2021-08-10 | Danisco Us Inc. | células recombinantes capazes de maior produção de isopreno, mevalonato e isoprenoides e método para produzir isopreno, mevalonato e isoprenoides |
| SG194691A1 (en) * | 2011-04-29 | 2013-12-30 | Danisco Us Inc | Production of mevalonate, isoprene, and isoprenoids using genes encodingpolypeptides having thiolase, hmg-coa synthase and hmg-coa reductase enzymatic activities |
| PE20141519A1 (es) | 2011-07-12 | 2014-10-17 | Scientist Of Fortune Sa | Microorganismo recombinante para la produccion de metabolitos utiles |
| BR112014008278A2 (pt) | 2011-10-07 | 2017-04-18 | Danisco Us Inc | utilização de fosfocetolase na produção de mevalonato, precursores isoprenoides e isopreno |
| CN104039974B (zh) * | 2011-11-09 | 2016-10-12 | 阿迈瑞斯公司 | 乙酰辅酶a衍生的类异戊二烯的生产 |
| US8741613B2 (en) | 2012-05-16 | 2014-06-03 | Glycos Biotechnologies, Inc. | Microorganisms and processes for the production of isoprene |
| SG11201508186XA (en) | 2013-04-10 | 2015-11-27 | Danisco Us Inc | Phosphoketolases for improved production of acetyl coenzyme a-derived metabolites, isoprene, isoprenoid precursors, and isoprenoids |
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2014
- 2014-04-10 SG SG11201508186XA patent/SG11201508186XA/en unknown
- 2014-04-10 EP EP18175724.6A patent/EP3425049A1/en not_active Withdrawn
- 2014-04-10 BR BR112015025489A patent/BR112015025489A2/pt not_active Application Discontinuation
- 2014-04-10 WO PCT/US2014/033688 patent/WO2014169144A2/en not_active Ceased
- 2014-04-10 CN CN201480032280.2A patent/CN105555952A/zh active Pending
- 2014-04-10 EP EP14727659.6A patent/EP2984105B1/en active Active
- 2014-04-10 ES ES14727659T patent/ES2735331T3/es active Active
- 2014-04-10 US US14/783,391 patent/US10246694B2/en active Active
- 2014-04-10 JP JP2016507665A patent/JP6530375B2/ja active Active
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2019
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- 2019-05-16 JP JP2019093057A patent/JP2019162130A/ja active Pending
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2020
- 2020-09-24 US US16/948,592 patent/US11371035B2/en active Active
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