CN105555952A - 用于乙酰辅酶a-衍生代谢物、异戊二烯、类异戊二烯前体和类异戊二烯的改善生产的磷酸解酮酶 - Google Patents

用于乙酰辅酶a-衍生代谢物、异戊二烯、类异戊二烯前体和类异戊二烯的改善生产的磷酸解酮酶 Download PDF

Info

Publication number
CN105555952A
CN105555952A CN201480032280.2A CN201480032280A CN105555952A CN 105555952 A CN105555952 A CN 105555952A CN 201480032280 A CN201480032280 A CN 201480032280A CN 105555952 A CN105555952 A CN 105555952A
Authority
CN
China
Prior art keywords
recombinant cell
polypeptide
phosphoketolase
seq
sequence identity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201480032280.2A
Other languages
English (en)
Chinese (zh)
Inventor
Z·Q·贝克
J·W·穆诺斯
D·H·韦尔斯
J·姚
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Goodyear Tire and Rubber Co
Danisco USA Inc
Original Assignee
Goodyear Tire and Rubber Co
Danisco USA Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Goodyear Tire and Rubber Co, Danisco USA Inc filed Critical Goodyear Tire and Rubber Co
Publication of CN105555952A publication Critical patent/CN105555952A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/02—Aldehyde-lyases (4.1.2)
    • C12Y401/02009—Phosphoketolase (4.1.2.9)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201480032280.2A 2013-04-10 2014-04-10 用于乙酰辅酶a-衍生代谢物、异戊二烯、类异戊二烯前体和类异戊二烯的改善生产的磷酸解酮酶 Pending CN105555952A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361810696P 2013-04-10 2013-04-10
US61/810,696 2013-04-10
US201361834359P 2013-06-12 2013-06-12
US61/834,359 2013-06-12
PCT/US2014/033688 WO2014169144A2 (en) 2013-04-10 2014-04-10 Phosphoketolases foe improved production of acetyl coenzyme a-derived metabolites, isoprene, isoprenoid precurosors, and isoprenoids

Publications (1)

Publication Number Publication Date
CN105555952A true CN105555952A (zh) 2016-05-04

Family

ID=50877659

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480032280.2A Pending CN105555952A (zh) 2013-04-10 2014-04-10 用于乙酰辅酶a-衍生代谢物、异戊二烯、类异戊二烯前体和类异戊二烯的改善生产的磷酸解酮酶

Country Status (8)

Country Link
US (3) US10246694B2 (https=)
EP (2) EP3425049A1 (https=)
JP (2) JP6530375B2 (https=)
CN (1) CN105555952A (https=)
BR (1) BR112015025489A2 (https=)
ES (1) ES2735331T3 (https=)
SG (1) SG11201508186XA (https=)
WO (1) WO2014169144A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107841523A (zh) * 2017-10-19 2018-03-27 浙江大学 利用群体感应分子诱导从桦褐孔菌中提取三萜类物质方法

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8647642B2 (en) 2008-09-18 2014-02-11 Aviex Technologies, Llc Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment
SG11201508186XA (en) 2013-04-10 2015-11-27 Danisco Us Inc Phosphoketolases for improved production of acetyl coenzyme a-derived metabolites, isoprene, isoprenoid precursors, and isoprenoids
EP3741865B1 (en) * 2014-09-18 2024-03-13 Genomatica, Inc. Non-natural microbial organisms with improved energetic efficiency
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
BR112019019701A2 (pt) * 2017-03-24 2020-07-14 Danisco Us Inc. redução de acetato e glicerol em levedura modificada que tem um caminho de produção de etanol exógeno
WO2019113387A1 (en) 2017-12-07 2019-06-13 Zymergen Inc. Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation
JP2021506316A (ja) 2017-12-21 2021-02-22 ザイマージェン インコーポレイテッド ネペタラクトールオキシドレダクターゼ、ネペタラクトールシンターゼ、及びネペタラクトンを産生する能力がある微生物
CA3090055A1 (en) 2018-03-01 2019-09-06 Total Raffinage Chimie Metabolic pathways with increased carbon yield
CN109929883A (zh) * 2019-04-02 2019-06-25 烟台华康荣赞生物科技有限公司 重组酵母、构建方法和其在制备酪醇及衍生物中的应用
WO2021063958A1 (en) * 2019-09-30 2021-04-08 Global Bioenergies Improved means and methods for increasing the yield of acetyl-coa from glucose

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101090964A (zh) * 2004-08-10 2007-12-19 味之素株式会社 磷酸转酮酶用于生产有用的代谢物的用途
WO2011159853A1 (en) * 2010-06-18 2011-12-22 Butamax(Tm) Advanced Biofuels Llc Recombinant host cells comprising phosphoketolases
WO2012149491A2 (en) * 2011-04-29 2012-11-01 Danisco Us Inc. Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids
WO2012149469A1 (en) * 2011-04-29 2012-11-01 Danisco Us Inc. Production of mevalonate, isoprene, and isoprenoids using genes encoding polypeptides having thiolase, hmg-coa synthase and hmg-coa reductase enzymatic activities
US8415136B1 (en) * 2011-11-09 2013-04-09 Amyris, Inc. Production of acetyl-coenzyme a derived isoprenoids

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR669447A (fr) 1929-02-09 1929-11-15 Dispositif pour arrêter automatiquement un moteur d'automobile après une période de temps prédéterminée
DE19629568C1 (de) 1996-07-15 1998-01-08 Fraunhofer Ges Forschung Verfahren zur Herstellung von Isopren
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
JPWO2003095651A1 (ja) 2002-05-10 2005-09-15 協和醗酵工業株式会社 メバロン酸の製造方法
KR101148255B1 (ko) 2002-10-04 2012-08-08 다니스코 유에스 인크. 고수율을 갖는 1,3-프로판디올의 생물학적 제조 방법
DE602005020469D1 (de) * 2004-08-10 2010-05-20 Ajinomoto Kk Verwendung von phosphoketolase zur herstellung geeigneter metabolite
WO2007140339A2 (en) 2006-05-26 2007-12-06 Amyris Biotechnologies, Inc. Production of isoprenoids
JP4986547B2 (ja) 2006-09-04 2012-07-25 株式会社Ａｄｅｋａ 新規なアセトアセチルＣｏＡ合成酵素、それをコードするＤＮＡ配列、当該酵素の製造方法および当該酵素を利用したメバロン酸の製造方法
WO2009005704A1 (en) * 2007-07-03 2009-01-08 The Regents Of The University Of California Methods of increasing isoprenoid or isoprenoid precursor production
CN105368764B (zh) 2007-12-13 2019-03-12 丹尼斯科美国公司 用于生产异戊二烯的组合物和方法
US20110045563A1 (en) 2008-02-06 2011-02-24 The Regents Of The University Of California Short chain volatile isoprene hydrocarbon production using the mevalonic acid pathway in genetically engineered yeast and fungi
WO2009132220A2 (en) 2008-04-23 2009-10-29 Danisco Us Inc. Isoprene synthase variants for improved microbial production of isoprene
US7632995B2 (en) 2008-05-09 2009-12-15 Monsanto Technology Llc Plants and seeds of hybrid corn variety CH852179
MX318543B (es) 2008-07-02 2014-03-18 Danisco Us Inc Composiciones y metodos para producir isopreno libre de hidrocarburos de c5 bajo condicion de desacoplamiento y/o intervalos de operacion seguros.
HU3516U (en) 2008-07-31 2008-11-28 Zoltan Kuthi Anti-theft protection of draining for fuel tanks
WO2010031062A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using the archaeal lower mevalonate pathway
CA2737082A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using mevalonate kinase and isoprene synthase
EP2340312A2 (en) 2008-09-15 2011-07-06 Danisco US Inc. Conversion of prenyl derivatives to isoprene
CA2737223A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Reduction of carbon dioxide emission during isoprene production by fermentation
EP2344629B1 (en) 2008-09-15 2018-01-24 Danisco US Inc. Systems using cell culture for production of isoprene
MY161071A (en) 2008-12-30 2017-04-14 Danisco Us Inc Methods of producing isoprene and a co-product
US8324442B2 (en) 2009-03-03 2012-12-04 Amyris, Inc. Microbial derived isoprene and methods for making the same
US20100297749A1 (en) 2009-04-21 2010-11-25 Sapphire Energy, Inc. Methods and systems for biofuel production
AU2010238770A1 (en) 2009-04-23 2011-11-03 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
JP4760951B2 (ja) 2009-05-08 2011-08-31 トヨタ自動車株式会社 ブタノール生産能を有する組換え微生物及びブタノールの製造方法
TW201412988A (zh) 2009-06-17 2014-04-01 Danisco Us Inc 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造
TWI434921B (zh) 2009-06-17 2014-04-21 Danisco Us Inc 從生物異戊二烯組合物製造燃料成分之方法及系統
US20120252054A1 (en) 2009-09-15 2012-10-04 Sapphire Energy Inc System for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.
US8569562B2 (en) 2009-12-18 2013-10-29 Danisco Us Inc. Purification of isoprene from renewable resources
SG196782A1 (en) 2009-12-23 2014-02-13 Danisco Us Inc Compositions and methods for the increased production of isoprene and other products with 6 - phosphogluconolactonase (pgl)
CA2816306A1 (en) 2010-10-27 2012-05-03 Danisco Us Inc. Isoprene synthase variants for improved production of isoprene
PE20141519A1 (es) 2011-07-12 2014-10-17 Scientist Of Fortune Sa Microorganismo recombinante para la produccion de metabolitos utiles
BR112014008278A2 (pt) 2011-10-07 2017-04-18 Danisco Us Inc utilização de fosfocetolase na produção de mevalonato, precursores isoprenoides e isopreno
US8741613B2 (en) 2012-05-16 2014-06-03 Glycos Biotechnologies, Inc. Microorganisms and processes for the production of isoprene
SG11201508186XA (en) 2013-04-10 2015-11-27 Danisco Us Inc Phosphoketolases for improved production of acetyl coenzyme a-derived metabolites, isoprene, isoprenoid precursors, and isoprenoids

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101090964A (zh) * 2004-08-10 2007-12-19 味之素株式会社 磷酸转酮酶用于生产有用的代谢物的用途
WO2011159853A1 (en) * 2010-06-18 2011-12-22 Butamax(Tm) Advanced Biofuels Llc Recombinant host cells comprising phosphoketolases
WO2012149491A2 (en) * 2011-04-29 2012-11-01 Danisco Us Inc. Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids
WO2012149469A1 (en) * 2011-04-29 2012-11-01 Danisco Us Inc. Production of mevalonate, isoprene, and isoprenoids using genes encoding polypeptides having thiolase, hmg-coa synthase and hmg-coa reductase enzymatic activities
US8415136B1 (en) * 2011-11-09 2013-04-09 Amyris, Inc. Production of acetyl-coenzyme a derived isoprenoids

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MARTA PAPINI ET AL.: "Physiological characterization of recombinant Saccharomyces cerevisiae expressing the Aspergillus nidulans phosphoketolase pathway: validation of activity through 13C-based metabolic flux analysis", 《APPL MICROBIOL BIOTECHNOL》 *
XIANHUA YIN ET AL.: "The gene encoding xylulose-5-phosphate/fructose-6-phosphate phosphoketolase (xfp) is conserved among Bifidobacterium species within a more variable region of the genome and both are useful for strain identification", 《FEMS MICROBIOLOGY LETTERS》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107841523A (zh) * 2017-10-19 2018-03-27 浙江大学 利用群体感应分子诱导从桦褐孔菌中提取三萜类物质方法

Also Published As

Publication number Publication date
US20210139878A1 (en) 2021-05-13
JP2016519577A (ja) 2016-07-07
JP6530375B2 (ja) 2019-06-12
US10246694B2 (en) 2019-04-02
EP2984105A2 (en) 2016-02-17
WO2014169144A8 (en) 2015-10-15
WO2014169144A3 (en) 2014-12-04
BR112015025489A2 (pt) 2017-10-10
US20190367896A1 (en) 2019-12-05
SG11201508186XA (en) 2015-11-27
JP2019162130A (ja) 2019-09-26
US20160068831A1 (en) 2016-03-10
ES2735331T3 (es) 2019-12-18
WO2014169144A2 (en) 2014-10-16
US10988750B2 (en) 2021-04-27
US11371035B2 (en) 2022-06-28
EP2984105B1 (en) 2019-05-22
EP3425049A1 (en) 2019-01-09

Similar Documents

Publication Publication Date Title
US11371035B2 (en) Phosphoketolases for improved production of acetyl coenzyme A-derived metabolites, isoprene, isoprenoid precursors, and isoprenoid
JP6639606B2 (ja) メバロン酸、イソプレノイド前駆体、及びイソプレンの産生におけるホスホケトラーゼの使用
US10364443B2 (en) Production of mevalonate, isoprene, and isoprenoids using genes encoding polypeptides having thiolase, HMG-CoA synthase and HMG-CoA reductase enzymatic activities
US20160002672A1 (en) Production of isoprene, isoprenoid, and isoprenoid precursors using an alternative lower mevalonate pathway
CN103930541A (zh) 用于增强甲羟戊酸、异戊二烯和类异戊二烯的产量的重组微生物
CN104245927A (zh) 使用具有降低的ispa活性的宿主细胞提高异戊二烯的产量
BR122023000309B1 (pt) Célula recombinante e método de produção de isopreno, de precursores de isoprenoides, de isoprenoides e/ou de metabólitos derivados de acetil coenzima a

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20160504

WD01 Invention patent application deemed withdrawn after publication