ES2735331T3 - Fosfocetolasas para mejorar la producción de metabolitos derivados de acetil-coenzima A, isopreno, precursores de isoprenoides e isoprenoides - Google Patents
Fosfocetolasas para mejorar la producción de metabolitos derivados de acetil-coenzima A, isopreno, precursores de isoprenoides e isoprenoides Download PDFInfo
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- ES2735331T3 ES2735331T3 ES14727659T ES14727659T ES2735331T3 ES 2735331 T3 ES2735331 T3 ES 2735331T3 ES 14727659 T ES14727659 T ES 14727659T ES 14727659 T ES14727659 T ES 14727659T ES 2735331 T3 ES2735331 T3 ES 2735331T3
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
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| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
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| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
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| WO2009132220A2 (en) | 2008-04-23 | 2009-10-29 | Danisco Us Inc. | Isoprene synthase variants for improved microbial production of isoprene |
| US7632995B2 (en) | 2008-05-09 | 2009-12-15 | Monsanto Technology Llc | Plants and seeds of hybrid corn variety CH852179 |
| MX318543B (es) | 2008-07-02 | 2014-03-18 | Danisco Us Inc | Composiciones y metodos para producir isopreno libre de hidrocarburos de c5 bajo condicion de desacoplamiento y/o intervalos de operacion seguros. |
| HU3516U (en) | 2008-07-31 | 2008-11-28 | Zoltan Kuthi | Anti-theft protection of draining for fuel tanks |
| WO2010031062A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Increased isoprene production using the archaeal lower mevalonate pathway |
| CA2737082A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Increased isoprene production using mevalonate kinase and isoprene synthase |
| EP2340312A2 (en) | 2008-09-15 | 2011-07-06 | Danisco US Inc. | Conversion of prenyl derivatives to isoprene |
| CA2737223A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Reduction of carbon dioxide emission during isoprene production by fermentation |
| EP2344629B1 (en) | 2008-09-15 | 2018-01-24 | Danisco US Inc. | Systems using cell culture for production of isoprene |
| MY161071A (en) | 2008-12-30 | 2017-04-14 | Danisco Us Inc | Methods of producing isoprene and a co-product |
| US8324442B2 (en) | 2009-03-03 | 2012-12-04 | Amyris, Inc. | Microbial derived isoprene and methods for making the same |
| US20100297749A1 (en) | 2009-04-21 | 2010-11-25 | Sapphire Energy, Inc. | Methods and systems for biofuel production |
| AU2010238770A1 (en) | 2009-04-23 | 2011-11-03 | Danisco Us Inc. | Three-dimensional structure of isoprene synthase and its use thereof for generating variants |
| JP4760951B2 (ja) | 2009-05-08 | 2011-08-31 | トヨタ自動車株式会社 | ブタノール生産能を有する組換え微生物及びブタノールの製造方法 |
| TW201412988A (zh) | 2009-06-17 | 2014-04-01 | Danisco Us Inc | 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造 |
| TWI434921B (zh) | 2009-06-17 | 2014-04-21 | Danisco Us Inc | 從生物異戊二烯組合物製造燃料成分之方法及系統 |
| US20120252054A1 (en) | 2009-09-15 | 2012-10-04 | Sapphire Energy Inc | System for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp. |
| US8569562B2 (en) | 2009-12-18 | 2013-10-29 | Danisco Us Inc. | Purification of isoprene from renewable resources |
| SG196782A1 (en) | 2009-12-23 | 2014-02-13 | Danisco Us Inc | Compositions and methods for the increased production of isoprene and other products with 6 - phosphogluconolactonase (pgl) |
| US8871488B2 (en) | 2010-06-18 | 2014-10-28 | Butamax Advanced Biofuels Llc | Recombinant host cells comprising phosphoketolases |
| CA2816306A1 (en) | 2010-10-27 | 2012-05-03 | Danisco Us Inc. | Isoprene synthase variants for improved production of isoprene |
| BR112013027555A2 (pt) * | 2011-04-29 | 2021-08-10 | Danisco Us Inc. | células recombinantes capazes de maior produção de isopreno, mevalonato e isoprenoides e método para produzir isopreno, mevalonato e isoprenoides |
| SG194691A1 (en) * | 2011-04-29 | 2013-12-30 | Danisco Us Inc | Production of mevalonate, isoprene, and isoprenoids using genes encodingpolypeptides having thiolase, hmg-coa synthase and hmg-coa reductase enzymatic activities |
| PE20141519A1 (es) | 2011-07-12 | 2014-10-17 | Scientist Of Fortune Sa | Microorganismo recombinante para la produccion de metabolitos utiles |
| BR112014008278A2 (pt) | 2011-10-07 | 2017-04-18 | Danisco Us Inc | utilização de fosfocetolase na produção de mevalonato, precursores isoprenoides e isopreno |
| CN104039974B (zh) * | 2011-11-09 | 2016-10-12 | 阿迈瑞斯公司 | 乙酰辅酶a衍生的类异戊二烯的生产 |
| US8741613B2 (en) | 2012-05-16 | 2014-06-03 | Glycos Biotechnologies, Inc. | Microorganisms and processes for the production of isoprene |
| SG11201508186XA (en) | 2013-04-10 | 2015-11-27 | Danisco Us Inc | Phosphoketolases for improved production of acetyl coenzyme a-derived metabolites, isoprene, isoprenoid precursors, and isoprenoids |
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2014
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- 2014-04-10 EP EP18175724.6A patent/EP3425049A1/en not_active Withdrawn
- 2014-04-10 BR BR112015025489A patent/BR112015025489A2/pt not_active Application Discontinuation
- 2014-04-10 WO PCT/US2014/033688 patent/WO2014169144A2/en not_active Ceased
- 2014-04-10 CN CN201480032280.2A patent/CN105555952A/zh active Pending
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- 2014-04-10 ES ES14727659T patent/ES2735331T3/es active Active
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|---|---|
| US20210139878A1 (en) | 2021-05-13 |
| JP2016519577A (ja) | 2016-07-07 |
| JP6530375B2 (ja) | 2019-06-12 |
| US10246694B2 (en) | 2019-04-02 |
| EP2984105A2 (en) | 2016-02-17 |
| WO2014169144A8 (en) | 2015-10-15 |
| WO2014169144A3 (en) | 2014-12-04 |
| BR112015025489A2 (pt) | 2017-10-10 |
| US20190367896A1 (en) | 2019-12-05 |
| CN105555952A (zh) | 2016-05-04 |
| SG11201508186XA (en) | 2015-11-27 |
| JP2019162130A (ja) | 2019-09-26 |
| US20160068831A1 (en) | 2016-03-10 |
| WO2014169144A2 (en) | 2014-10-16 |
| US10988750B2 (en) | 2021-04-27 |
| US11371035B2 (en) | 2022-06-28 |
| EP2984105B1 (en) | 2019-05-22 |
| EP3425049A1 (en) | 2019-01-09 |
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