JP2016512437A5 - - Google Patents

Download PDF

Info

Publication number
JP2016512437A5
JP2016512437A5 JP2016501984A JP2016501984A JP2016512437A5 JP 2016512437 A5 JP2016512437 A5 JP 2016512437A5 JP 2016501984 A JP2016501984 A JP 2016501984A JP 2016501984 A JP2016501984 A JP 2016501984A JP 2016512437 A5 JP2016512437 A5 JP 2016512437A5
Authority
JP
Japan
Prior art keywords
nucleic acid
amplicons
target nucleic
methylation specific
detection reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2016501984A
Other languages
English (en)
Japanese (ja)
Other versions
JP2016512437A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2014/025854 external-priority patent/WO2014160117A1/en
Publication of JP2016512437A publication Critical patent/JP2016512437A/ja
Publication of JP2016512437A5 publication Critical patent/JP2016512437A5/ja
Pending legal-status Critical Current

Links

JP2016501984A 2013-03-14 2014-03-13 多重メチル化特異的増幅システムおよび方法 Pending JP2016512437A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361782370P 2013-03-14 2013-03-14
US61/782,370 2013-03-14
PCT/US2014/025854 WO2014160117A1 (en) 2013-03-14 2014-03-13 Multiplex methylation-specific amplification systems and methods

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2018244615A Division JP2019076100A (ja) 2013-03-14 2018-12-27 多重メチル化特異的増幅システムおよび方法

Publications (2)

Publication Number Publication Date
JP2016512437A JP2016512437A (ja) 2016-04-28
JP2016512437A5 true JP2016512437A5 (de) 2017-03-30

Family

ID=51529804

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2016501984A Pending JP2016512437A (ja) 2013-03-14 2014-03-13 多重メチル化特異的増幅システムおよび方法
JP2018244615A Withdrawn JP2019076100A (ja) 2013-03-14 2018-12-27 多重メチル化特異的増幅システムおよび方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2018244615A Withdrawn JP2019076100A (ja) 2013-03-14 2018-12-27 多重メチル化特異的増幅システムおよび方法

Country Status (5)

Country Link
US (2) US9701999B2 (de)
EP (1) EP2971171A4 (de)
JP (2) JP2016512437A (de)
CN (1) CN105378107A (de)
WO (1) WO2014160117A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107922941A (zh) * 2015-06-15 2018-04-17 塞弗德公司 在自动化反应盒中DNA甲基化的整合的纯化和测量以及突变和/或mRNA表达水平的共同测量
CN108350485A (zh) 2015-10-30 2018-07-31 精密科学发展有限责任公司 血浆dna的多重扩增检测测定以及分离和检测
KR102436270B1 (ko) 2016-05-05 2022-08-25 이그젝트 싸이언스 디블롭먼트 컴패니, 엘엘씨 메틸화된 dna 분석에 의한 폐 종양의 검출
EP3551332B1 (de) 2016-12-12 2024-01-10 Cepheid Integrierte reinigung und messung der dna-methylierung und ko-messung von mutationen und/oder mrna-expressionsniveaus in einer automatisierten reaktionskartusche
WO2018140781A1 (en) 2017-01-27 2018-08-02 Exact Sciences Development Company, Llc Detection of colon neoplasia by analysis of methylated dna
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
US20230046033A1 (en) * 2019-09-16 2023-02-16 Exact Sciences Corporation Structure and temperature-dependent flap endonuclease substrates

Family Cites Families (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
EP0232967B1 (de) 1986-01-10 1993-04-28 Amoco Corporation Kompetitiver homogener Test
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5283174A (en) 1987-09-21 1994-02-01 Gen-Probe, Incorporated Homogenous protection assay
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5480784A (en) 1989-07-11 1996-01-02 Gen-Probe Incorporated Nucleic acid sequence amplification methods
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
SE501439C2 (sv) 1993-06-22 1995-02-13 Pharmacia Lkb Biotech Sätt och anordning för analys av polynukleotidsekvenser
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
KR100392057B1 (ko) 1993-11-30 2003-10-30 맥길 유니버시티 세포의 CpG 디뉴클레오티드 내의 시토신의 메틸화를 감소하는 방법
US5714330A (en) 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
JP3102800B2 (ja) 1994-08-19 2000-10-23 パーキン−エルマー コーポレイション 増幅及び連結反応の共役法
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
JP3189000B2 (ja) 1994-12-01 2001-07-16 東ソー株式会社 特定核酸配列の検出方法
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
DE69738687D1 (de) 1996-04-12 2008-06-26 Phri Properties Inc Sonden, kits und assays
US5871917A (en) 1996-05-31 1999-02-16 North Shore University Hospital Research Corp. Identification of differentially methylated and mutated nucleic acids
US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6017704A (en) 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6432360B1 (en) 1997-10-10 2002-08-13 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
DE19754482A1 (de) 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
AU762728B2 (en) 1998-07-02 2003-07-03 Gen-Probe Incorporated Molecular torches
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6303305B1 (en) 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
DE60014762T2 (de) 1999-05-24 2005-10-13 Tosoh Corp., Shinnanyo Methode zum Nachweis von Ribonukleinsäuren
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
EP1218543A2 (de) 1999-09-29 2002-07-03 Solexa Ltd. Polynukleotidsequenzierung
DE19959691A1 (de) * 1999-12-06 2001-08-16 Epigenomics Ag Verfahren zur parallelen Detektions des Methylierungszustandes von genomischer DNA
DE10010280B4 (de) 2000-02-25 2006-08-10 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
CA2415897A1 (en) 2000-07-07 2002-01-17 Susan H. Hardin Real-time sequence determination
US6706162B1 (en) 2000-09-25 2004-03-16 Applera Corporation High speed, high resolution compositions, methods, and kits for capillary electrophoresis
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7668697B2 (en) 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US7250098B2 (en) 2001-09-28 2007-07-31 Applera Corporation Multi-capillary array electrophoresis device
DE10215770A1 (de) 2002-04-10 2003-10-30 Michael Giesing Verfahren zum Nachweis methylierter und/oder nichtmethylierter Cytosine in Nukleinsäuren, dessen Verwendung sowie entsprechende Analysekits
AU2003290433A1 (en) * 2002-12-25 2004-07-22 Combimatrix K.K. Nucleic acid methylation detection process using an internal reference sample
EP1590477B1 (de) 2003-01-29 2009-07-29 454 Corporation Verfahren zur amplifikation und sequenzierung von nukleinsäuren
AU2004235747B2 (en) 2003-05-01 2009-05-28 Gen-Probe Incorporated Oligonucleotides comprising a molecular switch
WO2005042713A2 (en) * 2003-10-28 2005-05-12 The Johns Hopkins University Quantitative multiplex methylation-specific pcr
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
AU2005280162B2 (en) 2004-08-27 2012-04-26 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
AU2005308916B2 (en) * 2004-11-29 2011-07-21 Sequenom, Inc. Kits and methods for detecting methylated DNA
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
EP2230316A1 (de) 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Nukleinsäuresequenzierung durch schrittweise Duplexverlängerung
WO2007032748A1 (en) 2005-09-15 2007-03-22 Agency For Science, Technology & Research Method for detecting dna methylation
US7282337B1 (en) 2006-04-14 2007-10-16 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US20080241951A1 (en) 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Method and apparatus for moving stage detection of single molecular events
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
ES2923759T3 (es) 2006-12-14 2022-09-30 Life Technologies Corp Aparato para medir analitos utilizando matrices de FET
WO2008128198A2 (en) * 2007-04-12 2008-10-23 Usc Stevens - University Of Southern California Dna methylation analysis by digital bisulfite genomic sequencing and digital methylight
ES2559313T3 (es) 2007-06-19 2016-02-11 Stratos Genomics Inc. Secuenciación de ácidos nucleicos de alto rendimiento por expansión
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20110091882A1 (en) * 2009-10-02 2011-04-21 Ibis Biosciences, Inc. Determination of methylation status of polynucleotides
CN102424857B (zh) 2011-12-30 2013-11-27 陕西北美基因股份有限公司 一种Taqman水解探针以及定量检测MGMT甲基化程度的方法

Similar Documents

Publication Publication Date Title
JP2016512437A5 (de)
JP2015156872A5 (de)
JP2016195614A5 (de)
ES2610461T3 (es) Método de detección de nucleótidos únicos
SA516371691B1 (ar) Dna طرق وتركيبات لتحديد صورة تحليلية لـ
WO2016154337A3 (en) Method for identification and enumeration of nucleic acid sequences
JP2019528704A5 (de)
RU2017101353A (ru) Набор или устройство для обнаружения рака пищевода и способ обнаружения
ATE549419T1 (de) Verfahren und zusammensetzungen für die universelle grössenspezifische polymerasekettenreaktion
RU2017101174A (ru) Набор или устройство для обнаружения рака печени и способ обнаружения
NZ723570A (en) Compositions and methods for quantifying a nucleic acid sequence in a sample
WO2016059473A3 (en) Sequence conversion and signal amplifier dna having locked nucleic acids and detection methods using same
BR112016029907A2 (pt) enriquecimento geneticamente adaptado e reação em cadeia da polimerase para a rápida detecção de microrganismos".
WO2018089945A8 (en) Polynucleotides for the amplification and detection of neisseria gonorrhoeae
NZ732886A (en) Amplification and detection of nucleic acids in a biological sample
JP2017501733A5 (de)
EA201491821A1 (ru) Модифицированные олигонуклеотиды, включающие тиольные функциональные группы, и их применение для детекции нуклеиновых кислот
WO2015153571A3 (en) Detection of dna methylation using combined nuclease ligation reactions
JP2014083052A5 (de)
WO2015127201A8 (en) Hiv-2 nucleic acids and methods of detection
WO2016073255A8 (en) Detection of hiv-1 nucleic acids by reverse-transcription loop-mediated isothermal amplication
MY182821A (en) Kit for together detecting multiple target nucleic acids differing from each other and detection method using the same
JP2014003946A5 (de)
EP3342876A3 (de) Verfahren zum nachweis von nukleinsäuren gastrointestinaler pathogene
MX2019011503A (es) Composiciones de cebador y métodos multiplex para detectar la presencia de listeria.