JP2015537212A - イオン強度媒介のｐｈ勾配イオン交換クロマトグラフィー - Google Patents

イオン強度媒介のｐｈ勾配イオン交換クロマトグラフィー Download PDF

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Publication number
JP2015537212A
JP2015537212A JP2015542849A JP2015542849A JP2015537212A JP 2015537212 A JP2015537212 A JP 2015537212A JP 2015542849 A JP2015542849 A JP 2015542849A JP 2015542849 A JP2015542849 A JP 2015542849A JP 2015537212 A JP2015537212 A JP 2015537212A
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polypeptide
gradient
antibody
ionic strength
contaminants
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JP2015537212A5 (enExample
Inventor
ヤーチュン ワン，
ヤーチュン ワン，
ジョージ ティー． モレノ，
ジョージ ティー． モレノ，
ボヤン チャン，
ボヤン チャン，
リャンイー チャン，
リャンイー チャン，
デル ファーナン，
デル ファーナン，
トム パータポフ，
トム パータポフ，
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Genentech Inc
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Genentech Inc
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Publication of JP2015537212A publication Critical patent/JP2015537212A/ja
Publication of JP2015537212A5 publication Critical patent/JP2015537212A5/ja
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2015542849A 2012-11-15 2013-11-15 イオン強度媒介のｐｈ勾配イオン交換クロマトグラフィー Pending JP2015537212A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261727051P 2012-11-15 2012-11-15
US61/727,051 2012-11-15
US201361780707P 2013-03-13 2013-03-13
US61/780,707 2013-03-13
PCT/US2013/070415 WO2014078729A1 (en) 2012-11-15 2013-11-15 IONIC STRENGTH-MEDIATED pH GRADIENT ION EXCHANGE CHROMATOGRAPHY

Publications (2)

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JP2015537212A true JP2015537212A (ja) 2015-12-24
JP2015537212A5 JP2015537212A5 (enExample) 2016-12-28

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JP2015542849A Pending JP2015537212A (ja) 2012-11-15 2013-11-15 イオン強度媒介のｐｈ勾配イオン交換クロマトグラフィー

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US (2) US9810670B2 (enExample)
EP (1) EP2920586A4 (enExample)
JP (1) JP2015537212A (enExample)
KR (1) KR20150084046A (enExample)
CN (1) CN105051528A (enExample)
BR (1) BR112015011011A2 (enExample)
CA (1) CA2890979A1 (enExample)
HK (1) HK1217232A1 (enExample)
MX (1) MX2015006149A (enExample)
RU (1) RU2015122726A (enExample)
WO (1) WO2014078729A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021210662A1 (ja) * 2020-04-17 2021-10-21 小野薬品工業株式会社 タンパク質製剤原薬の着色除去方法
JP2022547203A (ja) * 2019-09-25 2022-11-10 エフ．ホフマン－ラ ロシュ アーゲー 予測される溶出緩衝液の塩濃度を使用するカチオンクロマトグラフィー

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WO2014078729A1 (en) 2012-11-15 2014-05-22 Genentech, Inc. IONIC STRENGTH-MEDIATED pH GRADIENT ION EXCHANGE CHROMATOGRAPHY
ES2705700T3 (es) * 2013-07-12 2019-03-26 Hoffmann La Roche Elucidación de la optimización de entrada en cromatografía de intercambio iónico
EP3280444B1 (en) * 2015-04-10 2024-05-08 Adimab, LLC Methods for purifying heterodimeric multispecific antibodies from parental homodimeric antibody species
GB2539420B (en) * 2015-06-16 2021-01-13 Cytiva Sweden Ab Determination of chromatography conditions
WO2017084738A1 (en) * 2015-11-18 2017-05-26 Merck Patent Gmbh Opposite ph-salt gradients for improved protein separations
KR20180083409A (ko) * 2015-11-18 2018-07-20 메르크 파텐트 게엠베하 이온 교환 크로마토그래피에서의 개선된 단백질 분리
CN108333264A (zh) * 2017-01-20 2018-07-27 湖北生物医药产业技术研究院有限公司 检测蛋白电荷变异体的方法及确定生物制品生产工艺的方法
WO2019067637A1 (en) * 2017-09-26 2019-04-04 Waters Technologies Corporation HIGH-PURITY CHROMATOGRAPHIC MATERIALS COMPRISING AN IONIZABLE MODIFIER FOR RETENTION OF ACID ANALYTES
EP3791172B1 (en) 2018-05-08 2023-06-28 Waters Technologies Corporation Methods, compositions and kits useful for ph gradient cation exchange chromatography
KR20200080748A (ko) * 2018-12-27 2020-07-07 주식회사 폴루스 음이온 교환 크로마토그래피를 이용한 인슐린 전구체의 정제방법
CN111024883B (zh) * 2019-12-31 2022-12-06 上海博威生物医药有限公司 一种奥马珠单抗的电荷异质体的分离方法
CN111239278B (zh) * 2020-02-05 2022-11-04 康立泰生物医药(青岛)有限公司 一种检测重组人白介素-12蛋白电荷变异体的方法及应用
KR102283871B1 (ko) * 2021-03-02 2021-08-02 한국원자력연구원 크로마토그래피를 이용한 무담체 홀뮴-166의 분리 방법, 분리 장치 및 이에 의하여 분리된 무담체 홀뮴-166
JP2024527758A (ja) * 2021-07-13 2024-07-26 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 陰イオン交換クロマトグラフィー－質量分析（ａｅｘ－ｍｓ）によるタンパク質の特徴付け方法
WO2023107510A1 (en) * 2021-12-06 2023-06-15 Staveness Daryl Exciplex-based methods for contaminant and disease detection and methods for use of extraction buffers
CN114544839B (zh) * 2022-01-20 2024-08-20 未名生物医药有限公司 一种抗人神经生长因子抗体的电荷变异体检测方法
WO2024134512A1 (en) * 2022-12-20 2024-06-27 Seqirus Inc. Lipid nanoparticle composition
WO2024261724A1 (en) * 2023-06-23 2024-12-26 Lupin Limited Ion-exchange chromatography for separation and analysis of charge variants
WO2025038736A1 (en) * 2023-08-15 2025-02-20 Gigagen, Inc. Methods of analyzing recombinant polyclonal proteins

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Cited By (5)

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Publication number Priority date Publication date Assignee Title
JP2022547203A (ja) * 2019-09-25 2022-11-10 エフ．ホフマン－ラ ロシュ アーゲー 予測される溶出緩衝液の塩濃度を使用するカチオンクロマトグラフィー
JP7598929B2 (ja) 2019-09-25 2024-12-12 エフ． ホフマン－ラ ロシュ アーゲー 予測される溶出緩衝液の塩濃度を使用するカチオンクロマトグラフィー
WO2021210662A1 (ja) * 2020-04-17 2021-10-21 小野薬品工業株式会社 タンパク質製剤原薬の着色除去方法
JPWO2021210662A1 (enExample) * 2020-04-17 2021-10-21
JP7686180B2 (ja) 2020-04-17 2025-06-02 小野薬品工業株式会社 タンパク質製剤原薬の着色除去方法

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HK1217232A1 (zh) 2016-12-30
CA2890979A1 (en) 2014-05-22
US20150285771A1 (en) 2015-10-08
RU2015122726A (ru) 2017-01-10
CN105051528A (zh) 2015-11-11
BR112015011011A2 (pt) 2019-12-17
MX2015006149A (es) 2015-08-05
WO2014078729A1 (en) 2014-05-22
EP2920586A1 (en) 2015-09-23
EP2920586A4 (en) 2017-01-04
US10712322B2 (en) 2020-07-14
KR20150084046A (ko) 2015-07-21
US20180120273A1 (en) 2018-05-03
US9810670B2 (en) 2017-11-07

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