JP2015519892A5 - - Google Patents

Info

Publication number

JP2015519892A5

JP2015519892A5 JP2015509459A JP2015509459A JP2015519892A5 JP 2015519892 A5 JP2015519892 A5 JP 2015519892A5 JP 2015509459 A JP2015509459 A JP 2015509459A JP 2015509459 A JP2015509459 A JP 2015509459A JP 2015519892 A5 JP2015519892 A5 JP 2015519892A5

Authority

JP

Japan

Prior art keywords

adenine dinucleotide

flavin adenine

glucose dehydrogenase

dependent glucose

modified flavin

Prior art date

2013-05-03

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 230000001419 dependent effect Effects 0.000 claims 29
• VWWQXMAJTJZDQX-UYBVJOGSSA-N flavin adenine dinucleotide Chemical class C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1CO[P@](O)(=O)O[P@@](O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C2=NC(=O)NC(=O)C2=NC2=C1C=C(C)C(C)=C2 VWWQXMAJTJZDQX-UYBVJOGSSA-N 0.000 claims 29
• 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 claims 26
• 239000012634 fragment Substances 0.000 claims 20
• 230000013595 glycosylation Effects 0.000 claims 10
• 238000006206 glycosylation reaction Methods 0.000 claims 10
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical class C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 8
• 240000006439 Aspergillus oryzae Species 0.000 claims 7
• 235000002247 Aspergillus oryzae Nutrition 0.000 claims 7
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 5
• 239000008103 glucose Substances 0.000 claims 5
• 239000002157 polynucleotide Substances 0.000 claims 5
• 102000040430 polynucleotide Human genes 0.000 claims 5
• 108091033319 polynucleotide Proteins 0.000 claims 5
• 238000006467 substitution reaction Methods 0.000 claims 5
• 108090000790 Enzymes Proteins 0.000 claims 4
• 102000004190 Enzymes Human genes 0.000 claims 4
• 229940024606 amino acid Drugs 0.000 claims 3
• 235000001014 amino acid Nutrition 0.000 claims 3
• 150000001413 amino acids Chemical class 0.000 claims 3
• 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 claims 3
• 238000001514 detection method Methods 0.000 claims 3
• 235000019162 flavin adenine dinucleotide Nutrition 0.000 claims 3
• 239000011714 flavin adenine dinucleotide Substances 0.000 claims 3
• 229940093632 flavin-adenine dinucleotide Drugs 0.000 claims 3
• 125000003147 glycosyl group Chemical group 0.000 claims 3
• 238000005259 measurement Methods 0.000 claims 3
• 230000000694 effects Effects 0.000 claims 2
• 239000013604 expression vector Substances 0.000 claims 2
• 238000000034 method Methods 0.000 claims 2
• 101710088194 Dehydrogenase Proteins 0.000 claims 1
• 241000588724 Escherichia coli Species 0.000 claims 1
• 230000004988 N-glycosylation Effects 0.000 claims 1
• 125000003275 alpha amino acid group Chemical group 0.000 claims 1
• 238000012258 culturing Methods 0.000 claims 1
• 230000018044 dehydration Effects 0.000 claims 1
• 238000006297 dehydration reaction Methods 0.000 claims 1
• 238000006356 dehydrogenation reaction Methods 0.000 claims 1
• 230000008030 elimination Effects 0.000 claims 1
• 238000003379 elimination reaction Methods 0.000 claims 1
• 230000002255 enzymatic effect Effects 0.000 claims 1
• 230000001747 exhibiting effect Effects 0.000 claims 1
• 238000004108 freeze drying Methods 0.000 claims 1
• 238000011534 incubation Methods 0.000 claims 1
• 238000002955 isolation Methods 0.000 claims 1
• 238000004519 manufacturing process Methods 0.000 claims 1
• 239000002808 molecular sieve Substances 0.000 claims 1
• 239000002773 nucleotide Substances 0.000 claims 1
• 125000003729 nucleotide group Chemical group 0.000 claims 1
• URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 claims 1
• 125000003944 tolyl group Chemical group 0.000 claims 1