KR101850178B1 - 글리코실화된 수식된 플라빈 아데닌 디뉴클레오티드 의존성 글루코스 탈수소효소 - Google Patents

글리코실화된 수식된 플라빈 아데닌 디뉴클레오티드 의존성 글루코스 탈수소효소 Download PDF

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KR101850178B1
KR101850178B1 KR1020147030170A KR20147030170A KR101850178B1 KR 101850178 B1 KR101850178 B1 KR 101850178B1 KR 1020147030170 A KR1020147030170 A KR 1020147030170A KR 20147030170 A KR20147030170 A KR 20147030170A KR 101850178 B1 KR101850178 B1 KR 101850178B1
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adenine dinucleotide
ala
gly
glucose dehydrogenase
leu
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KR20150001775A (ko
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하르트무트 뒤펠
토마스 마이어
미하엘 타케
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에프. 호프만-라 로슈 아게
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/99—Oxidoreductases acting on the CH-OH group of donors (1.1) with other acceptors (1.1.99)
    • C12Y101/9901—Glucose dehydrogenase (acceptor) (1.1.99.10)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/05—Oxidoreductases acting on the CH-OH group of donors (1.1) with a quinone or similar compound as acceptor (1.1.5)

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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020147030170A 2012-05-03 2013-05-03 글리코실화된 수식된 플라빈 아데닌 디뉴클레오티드 의존성 글루코스 탈수소효소 Active KR101850178B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12166703.4 2012-05-03
EP12166703 2012-05-03
PCT/EP2013/059313 WO2013164477A1 (en) 2012-05-03 2013-05-03 A glycosylated modified flavin adenine dinucleotide dependent glucose dehydrogenase

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KR20150001775A KR20150001775A (ko) 2015-01-06
KR101850178B1 true KR101850178B1 (ko) 2018-05-31

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KR1020147030170A Active KR101850178B1 (ko) 2012-05-03 2013-05-03 글리코실화된 수식된 플라빈 아데닌 디뉴클레오티드 의존성 글루코스 탈수소효소

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US (1) US9637774B2 (enExample)
EP (1) EP2844747B1 (enExample)
JP (1) JP6258298B2 (enExample)
KR (1) KR101850178B1 (enExample)
CN (1) CN104271736B (enExample)
CA (1) CA2869527C (enExample)
WO (1) WO2013164477A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2647705B1 (en) 2010-12-02 2016-08-03 Kikkoman Corporation Flavin-bound glucose dehyrogenase, production method for flavin-bound glucose dehyrogenase, and yeast transformant used in same
JP6526572B2 (ja) * 2013-12-27 2019-06-05 キッコーマン株式会社 熱安定性が向上したフラビン結合型グルコースデヒドロゲナーゼ
KR20180040595A (ko) * 2015-07-23 2018-04-20 스마트자임 바이오파마 리미티드 혈당치를 측정하기 위한 조성물 및 방법
JP6901717B2 (ja) * 2016-08-29 2021-07-14 国立研究開発法人産業技術総合研究所 グルコースセンサ用試薬、グルコースセンサ、グルコースセンサの製造方法、および、グルコース測定装置
KR102728752B1 (ko) * 2017-03-31 2024-11-11 기꼬만 가부시키가이샤 Fad-의존형 글루코오스 탈수소효소를 이용하는 연속적인 글루코오스 모니터링 방법
JP2021078354A (ja) 2018-03-08 2021-05-27 有限会社アルティザイム・インターナショナル フラビンアデニンジヌクレオチドグルコース脱水素酵素とシトクロム分子との融合タンパク質
CN108486027A (zh) * 2018-04-04 2018-09-04 江南大学 一种fad为辅基的葡萄糖脱氢酶的生产、纯化方法
CN112851821B (zh) * 2021-01-12 2022-07-05 山东省科学院生物研究所 一种融合酶及所述融合酶在纸基生物传感器中的应用
CN113552198B (zh) * 2021-05-31 2024-03-26 武汉维尔博生物科技有限公司 一种基于高响应葡萄糖适配体的电化学传感器及其制备方法
EP4512395A1 (en) 2023-08-21 2025-02-26 Bio Even Composition comprising flavin adenine dinucleotide (fad), l-gsh, atp and myristic acid, alone or with a drug

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080220460A1 (en) * 2006-11-14 2008-09-11 Toyo Boseki Kabushiki Kaisha Modified flavin adenine dinucleotide dependent glucose dehydrogenase
US20110045513A1 (en) * 2009-04-30 2011-02-24 Takenaka Ryo Protein electron mediator
JP2012029677A (ja) 2010-07-08 2012-02-16 Toyobo Co Ltd フラビンアデニンジヌクレオチド依存性グルコースデヒドロゲナーゼの基質特異性を改善するための方法
JP2012055229A (ja) 2010-09-09 2012-03-22 Toyobo Co Ltd フラビンアデニンジヌクレオチド依存性グルコースデヒドロゲナーゼの比活性を向上するための方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602007012130D1 (de) * 2006-11-14 2011-03-03 Toyo Boseki Modifizierte flavin-adenin-dinucleotid-abhängige glucose-dehydrogenase
JPWO2008102639A1 (ja) * 2007-02-20 2010-05-27 東洋紡績株式会社 グルコースの電気化学的定量方法、グルコース脱水素酵素組成物、及び、グルコース測定用電気化学センサー
CN101970656A (zh) * 2007-12-28 2011-02-09 池田食研株式会社 经修饰葡萄糖脱氢酶基因
JP5408125B2 (ja) * 2008-03-27 2014-02-05 東洋紡株式会社 糸状菌由来フラビンアデニンジヌクレオチド依存性グルコースデヒドロゲナーゼ（ｆａｄｇｄｈ）
JP2010054503A (ja) * 2008-08-01 2010-03-11 Toyobo Co Ltd グルコース脱水素酵素を用いたグルコースの電気化学測定法
JP2010057427A (ja) * 2008-09-04 2010-03-18 Toyobo Co Ltd グルコース脱水素酵素およびグルコースの電気化学測定法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080220460A1 (en) * 2006-11-14 2008-09-11 Toyo Boseki Kabushiki Kaisha Modified flavin adenine dinucleotide dependent glucose dehydrogenase
US20110045513A1 (en) * 2009-04-30 2011-02-24 Takenaka Ryo Protein electron mediator
JP2012029677A (ja) 2010-07-08 2012-02-16 Toyobo Co Ltd フラビンアデニンジヌクレオチド依存性グルコースデヒドロゲナーゼの基質特異性を改善するための方法
JP2012055229A (ja) 2010-09-09 2012-03-22 Toyobo Co Ltd フラビンアデニンジヌクレオチド依存性グルコースデヒドロゲナーゼの比活性を向上するための方法

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CN104271736B (zh) 2020-06-05
EP2844747B1 (en) 2019-03-20
JP2015519892A (ja) 2015-07-16
JP6258298B2 (ja) 2018-01-10
US20150064733A1 (en) 2015-03-05
WO2013164477A1 (en) 2013-11-07
CA2869527A1 (en) 2013-11-07
CN104271736A (zh) 2015-01-07
EP2844747A1 (en) 2015-03-11
US9637774B2 (en) 2017-05-02
KR20150001775A (ko) 2015-01-06
HK1205757A1 (en) 2015-12-24
CA2869527C (en) 2021-05-04

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