JP2015518169A - ｉｎ−ｓｉｔｕキャリブレーションを用いるゲル電気泳動用の組成物及び方法 - Google Patents

ｉｎ−ｓｉｔｕキャリブレーションを用いるゲル電気泳動用の組成物及び方法 Download PDF

Info

Publication number
JP2015518169A
JP2015518169A JP2015515158A JP2015515158A JP2015518169A JP 2015518169 A JP2015518169 A JP 2015518169A JP 2015515158 A JP2015515158 A JP 2015515158A JP 2015515158 A JP2015515158 A JP 2015515158A JP 2015518169 A JP2015518169 A JP 2015518169A
Authority
JP
Japan
Prior art keywords
calibrator
test sample
sample
lipoprotein
electrophoresis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2015515158A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015518169A5 (https=
Inventor
グアダーニョ，フィリップ
サマーズ，エリン
Original Assignee
ヘルス・ダイアグノスティック・ラボラトリー，インコーポレーテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ヘルス・ダイアグノスティック・ラボラトリー，インコーポレーテッド filed Critical ヘルス・ダイアグノスティック・ラボラトリー，インコーポレーテッド
Publication of JP2015518169A publication Critical patent/JP2015518169A/ja
Publication of JP2015518169A5 publication Critical patent/JP2015518169A5/ja
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/417—Systems using cells, i.e. more than one cell and probes with solid electrolytes
    • G01N27/4175—Calibrating or checking the analyser
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2015515158A 2012-05-29 2013-05-29 ｉｎ−ｓｉｔｕキャリブレーションを用いるゲル電気泳動用の組成物及び方法 Pending JP2015518169A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261652608P 2012-05-29 2012-05-29
US61/652,608 2012-05-29
US201361779567P 2013-03-13 2013-03-13
US61/779,567 2013-03-13
PCT/US2013/043140 WO2013181267A1 (en) 2012-05-29 2013-05-29 Compsition and method for gel electrophoresis with in-situ clalibration

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2017201797A Division JP7082467B2 (ja) 2012-05-29 2017-10-18 ｉｎ－ｓｉｔｕキャリブレーションを用いるゲル電気泳動用の組成物及び方法

Publications (2)

Publication Number Publication Date
JP2015518169A true JP2015518169A (ja) 2015-06-25
JP2015518169A5 JP2015518169A5 (https=) 2016-07-14

Family

ID=48699256

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2015515158A Pending JP2015518169A (ja) 2012-05-29 2013-05-29 ｉｎ−ｓｉｔｕキャリブレーションを用いるゲル電気泳動用の組成物及び方法
JP2017201797A Active JP7082467B2 (ja) 2012-05-29 2017-10-18 ｉｎ－ｓｉｔｕキャリブレーションを用いるゲル電気泳動用の組成物及び方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2017201797A Active JP7082467B2 (ja) 2012-05-29 2017-10-18 ｉｎ－ｓｉｔｕキャリブレーションを用いるゲル電気泳動用の組成物及び方法

Country Status (8)

Country Link
US (2) US9005419B2 (https=)
EP (1) EP2877846B1 (https=)
JP (2) JP2015518169A (https=)
CN (1) CN104508473B (https=)
AU (1) AU2013267450B2 (https=)
BR (1) BR112014029679A2 (https=)
CA (1) CA2874753C (https=)
WO (1) WO2013181267A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020528145A (ja) * 2017-07-21 2020-09-17 ヘレナ ラボラトリーズ コーポレーション 免疫固定法において抗血清を載置するためのシステム及び方法

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2686740T3 (es) * 2013-02-28 2018-10-19 Helena Laboratories Corporation Detección fluorescente in situ de apolipoproteínas de partículas lipídicas en la primera matraz electroforética
US9759682B2 (en) 2013-08-09 2017-09-12 True Health Ip Llc Applicator comb for gel electrophoresis
CA2933605A1 (en) 2013-12-18 2015-06-25 Philip Guadagno Lp(a) subform size identification using zonal gel immuno-fixation electrophoresis
CA2965172A1 (en) * 2014-10-21 2016-04-28 Philip Guadagno Quantitative molar concentration detection of specific apolipoprotein-containing particles present in bodily fluids by using capillary electrophoresis
WO2016065021A1 (en) * 2014-10-21 2016-04-28 True Health Diagnostics, Llc Lipoprotein particle number from measurements of lipoprotein particle phospholipid concentration in lipoprotein particle membrane bilayer
WO2016069639A1 (en) * 2014-10-27 2016-05-06 True Health Diagnostics, Llc Lp(a) subform size identification by capillary isoteachophoresis electrophoresis with laser-induced-fluorescence
EP3454964B1 (en) * 2016-05-13 2021-06-23 Agilent Technologies, Inc. Automated alignment, calibration and standardization of electrophoresis data
CN113227754B (zh) * 2018-08-16 2025-01-17 上海宜晟生物科技有限公司 使用智能监测结构的基于图像的测定
US20220099625A1 (en) 2019-03-02 2022-03-31 Helena Laboratories Corporation Applicator comb with serrated teeth for gel electrophoresis
US20220260596A1 (en) * 2019-06-27 2022-08-18 Case Western Reserve University Compositions and methods for blood anemia detection
EP3892989A1 (en) * 2020-04-07 2021-10-13 Ares Trading S.A. Capillary gel electrophoresis and its use with complex biological molecules
WO2023003444A1 (ko) * 2021-07-23 2023-01-26 주식회사 씨젠 핵산 반응 검출 장치용 캘리브레이션 플레이트를 준비하는 방법
CN113674367B (zh) * 2021-08-20 2024-03-26 上海宝藤生物医药科技股份有限公司 一种电泳后脂蛋白胆固醇试剂扫描图的预处理方法

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04232850A (ja) * 1990-07-11 1992-08-21 Genetype Ag 電気泳動分離のための内部標準
JPH10507514A (ja) * 1994-06-30 1998-07-21 オクラホマ メディカル リサーチ ファウンデーション リポタンパク質およびアポリポタンパク質に対する抗体ならびにその使用方法
US20040106153A1 (en) * 2002-07-18 2004-06-03 Montana State University Novel zwitterionic fluorescent dyes for labeling in proteomic and other biological analyses
JP2006526137A (ja) * 2003-05-28 2006-11-16 ジーイー・ヘルスケア・ユーケイ・リミテッド 内部標準の存在下で色素を標識することによる閉じた膜構造上の細胞表面タンパク質の示差分析
JP2007024610A (ja) * 2005-07-14 2007-02-01 Shimadzu Corp キャピラリー電気泳動方法
JP2008232927A (ja) * 2007-03-22 2008-10-02 Sharp Corp タンパク質濃縮装置及びタンパク質測定装置、これらを用いた空気調整機
JP2009168819A (ja) * 2008-01-17 2009-07-30 Korea Univ Research & Business Foundation 糖尿病性網膜症診断用のバイオマーカー
JP2011039002A (ja) * 2009-08-18 2011-02-24 Nec Corp マイクロ電気泳動チップおよび分析方法

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4741900A (en) 1982-11-16 1988-05-03 Cytogen Corporation Antibody-metal ion complexes
JPS6110772A (ja) * 1984-06-26 1986-01-18 Kyowa Medetsukusu Kk 生体成分の分析法
US5943295A (en) 1997-02-06 1999-08-24 Automotive Technologies International Inc. Method for identifying the presence and orientation of an object in a vehicle
US5571680A (en) * 1994-01-21 1996-11-05 Beckman Instruments, Inc. Homogeneous immunoassays and enzyme based assays of analytes using capillary electrophoresis
US5863401A (en) * 1994-04-14 1999-01-26 Beckman Instruments, Inc. Simultaneous analysis of analytes by immunoassay using capillary electophoresis with laser induced fluorescence
AU2550395A (en) 1994-06-02 1996-01-04 Perkin-Elmer Corporation, The Method and apparatus for automated carbohydrate mapping and sequencing
US5759375A (en) * 1996-05-17 1998-06-02 Purdue Research Foundation Miniaturized disposable gels for DNA analysis
GB9624927D0 (en) * 1996-11-29 1997-01-15 Oxford Glycosciences Uk Ltd Gels and their use
US5843295A (en) 1996-12-19 1998-12-01 Pharmacia Biotech Gel electrophoresis well-forming and loading-guide comb
US6863791B1 (en) 2000-09-11 2005-03-08 Spectrumedix Llc Method for in-situ calibration of electrophoretic analysis systems
JP3822454B2 (ja) * 2001-04-10 2006-09-20 独立行政法人科学技術振興機構 リン酸化蛋白質の同定方法
US6982029B2 (en) 2001-05-07 2006-01-03 Spectramedix Llc Electrophoretic method and system having internal lane standards for color calibration
EP2184369A1 (en) 2001-07-11 2010-05-12 Applied Biosystems, LLC Method for normalisation in electrophoresis
JP2003279539A (ja) * 2002-03-20 2003-10-02 Atoo Kk 穀物タンパク質に対して最適化した電気泳動法及びこれに用いる電気泳動用ゲル、電極緩衝液、試料抽出液。
US7136437B2 (en) * 2002-07-17 2006-11-14 Lucent Technologies Inc. Method and apparatus for receiving digital wireless transmissions using multiple-antenna communication schemes
EP1413520B1 (en) 2002-10-24 2006-08-02 Tetra Laval Holdings & Finance S.A. Sealing jaw
FR2862134B1 (fr) 2003-11-12 2007-07-27 Sebia Sa Analyse et typage de proteines monoclonales par electrophorese capillaire et immunodeplacement
JP4822409B2 (ja) * 2005-10-19 2011-11-24 独立行政法人産業技術総合研究所 タンパク質の分析方法
JP4684906B2 (ja) * 2006-02-09 2011-05-18 日本メナード化粧品株式会社 タイトジャンクション形成促進剤
US20090263829A1 (en) * 2006-03-14 2009-10-22 Washington University In St. Louis Alzheimer's disease biomarkers and methods of use
US20080227209A1 (en) * 2007-03-14 2008-09-18 David Xing-Fei Deng Methods, Kits And Devices For Analysis Of Lipoprotein(a)
KR100857592B1 (ko) * 2007-06-05 2008-09-09 충남대학교산학협력단 ＣｙＤｙｅ를 이용한 새로운 단백질 분석 방법
DK2112506T3 (en) * 2008-04-24 2016-05-30 Max Planck Ges Zur Förderung Der Wissenschaften E V Method for the automatic high-throughput identification of carbohydrates and carbohydrate composition of the diet patterns and systems should
US20090311677A1 (en) * 2008-06-13 2009-12-17 Andrew Reeves Identification of contaminating bacteria in industrial ethanol fermentations
CN101418350B (zh) * 2008-10-28 2011-06-15 南京农业大学 利用超低温技术脱除草莓轻型黄边病毒的方法
US9488666B2 (en) 2010-08-24 2016-11-08 Helena Laboratories Corporation Assay for determination of levels of lipoprotein particles in bodily fluids
ES2686740T3 (es) 2013-02-28 2018-10-19 Helena Laboratories Corporation Detección fluorescente in situ de apolipoproteínas de partículas lipídicas en la primera matraz electroforética

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04232850A (ja) * 1990-07-11 1992-08-21 Genetype Ag 電気泳動分離のための内部標準
JPH10507514A (ja) * 1994-06-30 1998-07-21 オクラホマ メディカル リサーチ ファウンデーション リポタンパク質およびアポリポタンパク質に対する抗体ならびにその使用方法
US20040106153A1 (en) * 2002-07-18 2004-06-03 Montana State University Novel zwitterionic fluorescent dyes for labeling in proteomic and other biological analyses
JP2006526137A (ja) * 2003-05-28 2006-11-16 ジーイー・ヘルスケア・ユーケイ・リミテッド 内部標準の存在下で色素を標識することによる閉じた膜構造上の細胞表面タンパク質の示差分析
JP2007024610A (ja) * 2005-07-14 2007-02-01 Shimadzu Corp キャピラリー電気泳動方法
JP2008232927A (ja) * 2007-03-22 2008-10-02 Sharp Corp タンパク質濃縮装置及びタンパク質測定装置、これらを用いた空気調整機
JP2009168819A (ja) * 2008-01-17 2009-07-30 Korea Univ Research & Business Foundation 糖尿病性網膜症診断用のバイオマーカー
JP2011039002A (ja) * 2009-08-18 2011-02-24 Nec Corp マイクロ電気泳動チップおよび分析方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020528145A (ja) * 2017-07-21 2020-09-17 ヘレナ ラボラトリーズ コーポレーション 免疫固定法において抗血清を載置するためのシステム及び方法
JP7366000B2 (ja) 2017-07-21 2023-10-20 ヘレナ ラボラトリーズ コーポレーション 免疫固定法において抗血清を載置するためのシステム及び方法

Also Published As

Publication number Publication date
CN104508473B (zh) 2017-10-13
US20130319864A1 (en) 2013-12-05
WO2013181267A8 (en) 2014-12-18
JP7082467B2 (ja) 2022-06-08
US10823698B2 (en) 2020-11-03
EP2877846A1 (en) 2015-06-03
WO2013181267A1 (en) 2013-12-05
US20150268193A1 (en) 2015-09-24
CA2874753A1 (en) 2013-12-05
CN104508473A (zh) 2015-04-08
JP2018028549A (ja) 2018-02-22
AU2013267450B2 (en) 2017-07-06
AU2013267450A1 (en) 2014-12-18
CA2874753C (en) 2023-02-28
BR112014029679A2 (pt) 2017-06-27
US9005419B2 (en) 2015-04-14
EP2877846B1 (en) 2026-04-15

Similar Documents

Publication Publication Date Title
JP7082467B2 (ja) ｉｎ－ｓｉｔｕキャリブレーションを用いるゲル電気泳動用の組成物及び方法
US20160252536A1 (en) Fluorescent in-situ detection of lipid particle apolipoproteins within primary electrophoretic matrix
JP5981433B2 (ja) 体液内のリポ蛋白粒子レベルを決定する分析
US10401370B2 (en) Lp(a) subform size identification using elisa
US10330690B2 (en) Lp(a) subform size identification by capillary isotachophoresis electrophoresis with laser-induced-fluorescence
WO2016065021A1 (en) Lipoprotein particle number from measurements of lipoprotein particle phospholipid concentration in lipoprotein particle membrane bilayer
CA2933605A1 (en) Lp(a) subform size identification using zonal gel immuno-fixation electrophoresis
US20160377618A1 (en) Pcsk9 quantification by immunodetection

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160527

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160527

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20170310

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170418

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170718

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20180129