BR112014029679A2 - método para realizar a eletroforese com calibração in situ - Google Patents

método para realizar a eletroforese com calibração in situ

Info

Publication number
BR112014029679A2
BR112014029679A2 BR112014029679A BR112014029679A BR112014029679A2 BR 112014029679 A2 BR112014029679 A2 BR 112014029679A2 BR 112014029679 A BR112014029679 A BR 112014029679A BR 112014029679 A BR112014029679 A BR 112014029679A BR 112014029679 A2 BR112014029679 A2 BR 112014029679A2
Authority
BR
Brazil
Prior art keywords
calibration
test sample
calibrator
fraction
volume
Prior art date
Application number
BR112014029679A
Other languages
English (en)
Inventor
Summers Erin
Guadagno Philip
Original Assignee
Health Diagnostic Laboratory Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Health Diagnostic Laboratory Inc filed Critical Health Diagnostic Laboratory Inc
Publication of BR112014029679A2 publication Critical patent/BR112014029679A2/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/417—Systems using cells, i.e. more than one cell and probes with solid electrolytes
    • G01N27/4175—Calibrating or checking the analyser
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1 / 1 resumo “mã‰todo para realizar a eletroforese com calibraã‡ãƒo in situ” a invenã§ã£o refere-se, entre outras coisas, a um mã©todo para realizar a eletroforese com calibraã§ã£o in situ. o mã©todo inclui combinar um volume de uma amostra de teste com um volume ou quantidade de uma amostra de calibraã§ã£o para formar um volume final, onde o volume da amostra de calibraã§ã£o inclui uma concentraã§ã£o conhecida de um calibrador e o volume final inclui uma razã£o conhecida da amostra de teste para a amostra de calibraã§ã£o. o mã©todo tambã©m inclui depositar uma fraã§ã£o de carga em um reservatã³rio de recepã§ã£o de um gel eletroforã©tico, em que a fraã§ã£o de carga ã© uma fraã§ã£o do volume final, e separar a fraã§ã£o de carga ao longo de uma linha de separaã§ã£o comum do gel eletroforã©tico tal que os componentes da amostra de teste e o calibrador estejam separados um do outro ao longo da linha de separaã§ã£o comum. o mã©todo tambã©m inclui detectar o calibrador e componentes separados da amostra de teste dentro da linha de separaã§ã£o comum e medir o nã­vel do calibrador e componentes separados da amostra de teste com base na detecã§ã£o, realizando deste modo a eletroforese com a calibraã§ã£o in situ.
BR112014029679A 2012-05-29 2013-05-29 método para realizar a eletroforese com calibração in situ BR112014029679A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261652608P 2012-05-29 2012-05-29
US201361779567P 2013-03-13 2013-03-13
PCT/US2013/043140 WO2013181267A1 (en) 2012-05-29 2013-05-29 Compsition and method for gel electrophoresis with in-situ clalibration

Publications (1)

Publication Number Publication Date
BR112014029679A2 true BR112014029679A2 (pt) 2017-06-27

Family

ID=48699256

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014029679A BR112014029679A2 (pt) 2012-05-29 2013-05-29 método para realizar a eletroforese com calibração in situ

Country Status (8)

Country Link
US (2) US9005419B2 (pt)
EP (1) EP2877846A1 (pt)
JP (2) JP2015518169A (pt)
CN (1) CN104508473B (pt)
AU (1) AU2013267450B2 (pt)
BR (1) BR112014029679A2 (pt)
CA (1) CA2874753C (pt)
WO (1) WO2013181267A1 (pt)

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EP3213088A1 (en) * 2014-10-27 2017-09-06 True Health IP LLC Lp(a) subform size identification by capillary isoteachophoresis electrophoresis with laser-induced-fluorescence
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Also Published As

Publication number Publication date
CA2874753A1 (en) 2013-12-05
CA2874753C (en) 2023-02-28
CN104508473A (zh) 2015-04-08
JP7082467B2 (ja) 2022-06-08
JP2015518169A (ja) 2015-06-25
US20130319864A1 (en) 2013-12-05
WO2013181267A8 (en) 2014-12-18
CN104508473B (zh) 2017-10-13
AU2013267450B2 (en) 2017-07-06
WO2013181267A1 (en) 2013-12-05
EP2877846A1 (en) 2015-06-03
US10823698B2 (en) 2020-11-03
US9005419B2 (en) 2015-04-14
JP2018028549A (ja) 2018-02-22
AU2013267450A1 (en) 2014-12-18
US20150268193A1 (en) 2015-09-24

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]