JP2015517816A5 - - Google Patents
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- Publication number
- JP2015517816A5 JP2015517816A5 JP2015514483A JP2015514483A JP2015517816A5 JP 2015517816 A5 JP2015517816 A5 JP 2015517816A5 JP 2015514483 A JP2015514483 A JP 2015514483A JP 2015514483 A JP2015514483 A JP 2015514483A JP 2015517816 A5 JP2015517816 A5 JP 2015517816A5
- Authority
- JP
- Japan
- Prior art keywords
- seq
- polynucleotide
- host cell
- fungal host
- intron
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091033319 polynucleotide Proteins 0.000 claims 34
- 239000002157 polynucleotide Substances 0.000 claims 34
- 102000040430 polynucleotide Human genes 0.000 claims 34
- 239000002773 nucleotide Substances 0.000 claims 24
- 125000003729 nucleotide group Chemical group 0.000 claims 24
- 210000004027 cell Anatomy 0.000 claims 23
- 230000002538 fungal effect Effects 0.000 claims 16
- 210000001324 spliceosome Anatomy 0.000 claims 14
- 108020004422 Riboswitch Proteins 0.000 claims 13
- 238000000034 method Methods 0.000 claims 8
- 229920001184 polypeptide Polymers 0.000 claims 8
- 108090000765 processed proteins & peptides Proteins 0.000 claims 8
- 102000004196 processed proteins & peptides Human genes 0.000 claims 8
- 240000006439 Aspergillus oryzae Species 0.000 claims 6
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims 6
- 108010059892 Cellulase Proteins 0.000 claims 6
- 102000004190 Enzymes Human genes 0.000 claims 6
- 108090000790 Enzymes Proteins 0.000 claims 6
- 102000004316 Oxidoreductases Human genes 0.000 claims 6
- 108090000854 Oxidoreductases Proteins 0.000 claims 6
- 229940088598 enzyme Drugs 0.000 claims 6
- 108090000623 proteins and genes Proteins 0.000 claims 6
- 241000534944 Thia Species 0.000 claims 5
- 101150026857 ctpJ gene Proteins 0.000 claims 5
- 241001494489 Thielavia Species 0.000 claims 4
- 241001149964 Tolypocladium Species 0.000 claims 4
- 241000222354 Trametes Species 0.000 claims 4
- 241000223259 Trichoderma Species 0.000 claims 4
- 239000003550 marker Substances 0.000 claims 4
- 108010011619 6-Phytase Proteins 0.000 claims 3
- 108090000915 Aminopeptidases Proteins 0.000 claims 3
- 102000004400 Aminopeptidases Human genes 0.000 claims 3
- 239000004382 Amylase Substances 0.000 claims 3
- 108010065511 Amylases Proteins 0.000 claims 3
- 102000013142 Amylases Human genes 0.000 claims 3
- 108010006303 Carboxypeptidases Proteins 0.000 claims 3
- 102000005367 Carboxypeptidases Human genes 0.000 claims 3
- 102000016938 Catalase Human genes 0.000 claims 3
- 108010053835 Catalase Proteins 0.000 claims 3
- 108010031396 Catechol oxidase Proteins 0.000 claims 3
- 102000030523 Catechol oxidase Human genes 0.000 claims 3
- 108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 claims 3
- 108010022172 Chitinases Proteins 0.000 claims 3
- 102000012286 Chitinases Human genes 0.000 claims 3
- 108010025880 Cyclomaltodextrin glucanotransferase Proteins 0.000 claims 3
- 108010053770 Deoxyribonucleases Proteins 0.000 claims 3
- 102000016911 Deoxyribonucleases Human genes 0.000 claims 3
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims 3
- 108090000371 Esterases Proteins 0.000 claims 3
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims 3
- 102100022624 Glucoamylase Human genes 0.000 claims 3
- 102000004157 Hydrolases Human genes 0.000 claims 3
- 108090000604 Hydrolases Proteins 0.000 claims 3
- 102000004195 Isomerases Human genes 0.000 claims 3
- 108090000769 Isomerases Proteins 0.000 claims 3
- 108010029541 Laccase Proteins 0.000 claims 3
- 108091026898 Leader sequence (mRNA) Proteins 0.000 claims 3
- 102000003960 Ligases Human genes 0.000 claims 3
- 108090000364 Ligases Proteins 0.000 claims 3
- 108090001060 Lipase Proteins 0.000 claims 3
- 239000004367 Lipase Substances 0.000 claims 3
- 102000004882 Lipase Human genes 0.000 claims 3
- 102000004317 Lyases Human genes 0.000 claims 3
- 108090000856 Lyases Proteins 0.000 claims 3
- 102100024295 Maltase-glucoamylase Human genes 0.000 claims 3
- 108010054377 Mannosidases Proteins 0.000 claims 3
- 102000001696 Mannosidases Human genes 0.000 claims 3
- 241000235395 Mucor Species 0.000 claims 3
- 241000226677 Myceliophthora Species 0.000 claims 3
- 241000233892 Neocallimastix Species 0.000 claims 3
- 108090000913 Nitrate Reductases Proteins 0.000 claims 3
- 108010025915 Nitrite Reductases Proteins 0.000 claims 3
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 3
- 108091005804 Peptidases Proteins 0.000 claims 3
- 102000035195 Peptidases Human genes 0.000 claims 3
- 102000003992 Peroxidases Human genes 0.000 claims 3
- 102000012288 Phosphopyruvate Hydratase Human genes 0.000 claims 3
- 108010022181 Phosphopyruvate Hydratase Proteins 0.000 claims 3
- 102000006382 Ribonucleases Human genes 0.000 claims 3
- 108010083644 Ribonucleases Proteins 0.000 claims 3
- 241000228341 Talaromyces Species 0.000 claims 3
- 241000228178 Thermoascus Species 0.000 claims 3
- 102000004357 Transferases Human genes 0.000 claims 3
- 108090000992 Transferases Proteins 0.000 claims 3
- 108060008539 Transglutaminase Proteins 0.000 claims 3
- 108010030291 alpha-Galactosidase Proteins 0.000 claims 3
- 102000005840 alpha-Galactosidase Human genes 0.000 claims 3
- 108010028144 alpha-Glucosidases Proteins 0.000 claims 3
- 235000019418 amylase Nutrition 0.000 claims 3
- 108010051210 beta-Fructofuranosidase Proteins 0.000 claims 3
- 108010005774 beta-Galactosidase Proteins 0.000 claims 3
- 102000005936 beta-Galactosidase Human genes 0.000 claims 3
- 108010047754 beta-Glucosidase Proteins 0.000 claims 3
- 102000006995 beta-Glucosidase Human genes 0.000 claims 3
- 108010089934 carbohydrase Proteins 0.000 claims 3
- 229940106157 cellulase Drugs 0.000 claims 3
- 108010005400 cutinase Proteins 0.000 claims 3
- 108010000165 exo-1,3-alpha-glucanase Proteins 0.000 claims 3
- 239000001573 invertase Substances 0.000 claims 3
- 235000011073 invertase Nutrition 0.000 claims 3
- 235000019421 lipase Nutrition 0.000 claims 3
- 229940072417 peroxidase Drugs 0.000 claims 3
- 108040007629 peroxidase activity proteins Proteins 0.000 claims 3
- 229940085127 phytase Drugs 0.000 claims 3
- 102000003601 transglutaminase Human genes 0.000 claims 3
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims 2
- 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 claims 2
- 241000228212 Aspergillus Species 0.000 claims 2
- 241000223651 Aureobasidium Species 0.000 claims 2
- 241000222490 Bjerkandera Species 0.000 claims 2
- 241000146399 Ceriporiopsis Species 0.000 claims 2
- 241000123346 Chrysosporium Species 0.000 claims 2
- 241000222511 Coprinus Species 0.000 claims 2
- 241000222356 Coriolus Species 0.000 claims 2
- 241001337994 Cryptococcus <scale insect> Species 0.000 claims 2
- 241000233866 Fungi Species 0.000 claims 2
- 241000223218 Fusarium Species 0.000 claims 2
- 241000223198 Humicola Species 0.000 claims 2
- 241001344133 Magnaporthe Species 0.000 claims 2
- 241000221960 Neurospora Species 0.000 claims 2
- 241000228143 Penicillium Species 0.000 claims 2
- 241000222385 Phanerochaete Species 0.000 claims 2
- 241000235379 Piromyces Species 0.000 claims 2
- 241000222350 Pleurotus Species 0.000 claims 2
- 241000222480 Schizophyllum Species 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 206010016165 failure to thrive Diseases 0.000 claims 2
- 101150095344 niaD gene Proteins 0.000 claims 2
- 241000222395 Phlebia Species 0.000 claims 1
- 230000010354 integration Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- -1 respectively Proteins 0.000 claims 1
- 230000009466 transformation Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12170260.9 | 2012-05-31 | ||
| EP12170260 | 2012-05-31 | ||
| PCT/EP2013/061052 WO2013178674A1 (en) | 2012-05-31 | 2013-05-29 | Improved selection in fungi |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015517816A JP2015517816A (ja) | 2015-06-25 |
| JP2015517816A5 true JP2015517816A5 (https=) | 2016-07-21 |
| JP6449763B2 JP6449763B2 (ja) | 2019-01-09 |
Family
ID=48539153
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015514483A Active JP6449763B2 (ja) | 2012-05-31 | 2013-05-29 | 真菌における改善された選択 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US9487767B2 (https=) |
| EP (2) | EP2855659A1 (https=) |
| JP (1) | JP6449763B2 (https=) |
| CN (1) | CN104395454B (https=) |
| BR (1) | BR112014029252B8 (https=) |
| DK (1) | DK3327113T3 (https=) |
| WO (1) | WO2013178674A1 (https=) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106068077B (zh) | 2014-03-05 | 2019-06-07 | 诺维信公司 | 用于改进农作物的收获后特性的组合物和方法 |
| WO2015134773A1 (en) | 2014-03-05 | 2015-09-11 | Novozymes A/S | Compositions and methods for functionalizing and linking materials |
| CN106062271A (zh) | 2014-03-05 | 2016-10-26 | 诺维信公司 | 用于改进具有木葡聚糖内糖基转移酶的纤维素纺织材料的性质的组合物和方法 |
| WO2015134729A1 (en) | 2014-03-05 | 2015-09-11 | Novozymes A/S | Compositions and methods for improving properties of non-cellulosic textile materials with xyloglucan endotransglycosylase |
| US20170027167A1 (en) | 2014-03-05 | 2017-02-02 | Novozymes Bioag A/S | Formulations comprising polymeric xyloglucan as a carrier for agriculturally beneficial agents |
| EP3183339A1 (en) | 2014-08-20 | 2017-06-28 | Novozymes A/S | Xyloglucan endotransglycosylase variants and polynucleotides encoding same |
| WO2016090059A1 (en) | 2014-12-02 | 2016-06-09 | Novozymes A/S | Laccase variants and polynucleotides encoding same |
| KR102699584B1 (ko) | 2015-02-02 | 2024-08-28 | 메이라지티엑스 유케이 Ii 리미티드 | 대안적 스플라이싱의 압타머 매개 조절에 의한 유전자 발현 제어 |
| EP3274461A1 (en) * | 2015-03-27 | 2018-01-31 | Cargill, Incorporated | Glucoamylase-modified yeast strains and methods for bioproduct production |
| WO2016174258A1 (en) | 2015-04-29 | 2016-11-03 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Means and methods for protein production |
| WO2017106739A1 (en) | 2015-12-17 | 2017-06-22 | Cargill, Incorporated | Sugar transporter-modified yeast strains and methods for bioproduct production |
| EP3494229B1 (en) | 2016-08-03 | 2023-10-25 | Meiragtx UK II Limited | High throughput cell-based screening for aptamers |
| WO2018027131A1 (en) | 2016-08-05 | 2018-02-08 | Cargill, Incorporated | Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production |
| CN108949579B (zh) * | 2018-04-19 | 2021-10-01 | 中国科学技术大学 | 嗜热子囊菌基因表达系统 |
| CN112513283B (zh) | 2018-06-15 | 2024-02-06 | 龟甲万株式会社 | 麦角硒因的制造方法 |
| EP3830261A1 (en) | 2018-08-02 | 2021-06-09 | Novozymes A/S | Preparation of combinatorial libraries of dna constructs using introns |
| CN113316641B (zh) | 2018-12-21 | 2025-01-28 | 诺维信公司 | 串联蛋白表达 |
| CN110592149B (zh) * | 2019-07-16 | 2020-07-14 | 甘肃省科学院生物研究所 | 一种梨囊鞭菌发酵秸秆生产乳酸的新方法和应用 |
| CN114391038A (zh) | 2019-07-25 | 2022-04-22 | 诺维信公司 | 丝状真菌表达系统 |
| US11479779B2 (en) * | 2020-07-31 | 2022-10-25 | Zymergen Inc. | Systems and methods for high-throughput automated strain generation for non-sporulating fungi |
| CN114292761B (zh) * | 2021-12-02 | 2023-11-03 | 南京工业大学 | 一株黑曲霉基因工程菌及构建方法与应用 |
| WO2025133003A2 (en) | 2023-12-19 | 2025-06-26 | 21St.Bio A/S | Non-animal bovine beta lactoglobulin |
| CN119752977A (zh) * | 2023-12-28 | 2025-04-04 | 南京百斯杰生物工程有限公司 | 可改善目标多肽表达效果的多核苷酸构建体及其应用 |
| WO2025226596A1 (en) | 2024-04-24 | 2025-10-30 | Novozymes A/S | Methods for producing secreted polypeptides |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
| FI964808A0 (fi) | 1994-06-03 | 1996-12-02 | Novo Nordisk Biotech Inc | Puhdistetut Myceliophthora lakkaasit ja niitä koodaavat nukleiinihapot |
| CN101659926A (zh) | 1994-06-30 | 2010-03-03 | 诺沃奇梅兹有限公司 | 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子 |
| JP4436934B2 (ja) | 1996-09-13 | 2010-03-24 | ノボザイムス，インコーポレイティド | 変更された量のポリペプチドを生成するｄｎａ挿入突然変異を有する細胞 |
| CN1197965C (zh) | 1998-10-26 | 2005-04-20 | 诺维信公司 | 在丝状真菌细胞内构建和筛选目的dna文库 |
| CN100510096C (zh) | 1999-03-22 | 2009-07-08 | 诺沃奇梅兹有限公司 | 用于在真菌细胞中表达基因的启动子 |
| US6762040B2 (en) | 2000-05-24 | 2004-07-13 | Novozymes A/S | Method for increasing gene copy number in a host cell and resulting host cell |
| JP2007259787A (ja) * | 2006-03-29 | 2007-10-11 | Hakutsuru Shuzo Kk | 遺伝子発現制御ポリヌクレオチド |
| GB201107768D0 (en) | 2011-05-10 | 2011-06-22 | Univ Manchester | Riboswitches |
-
2013
- 2013-05-29 JP JP2015514483A patent/JP6449763B2/ja active Active
- 2013-05-29 DK DK17200169.5T patent/DK3327113T3/da active
- 2013-05-29 CN CN201380028411.5A patent/CN104395454B/zh active Active
- 2013-05-29 EP EP13726188.9A patent/EP2855659A1/en not_active Withdrawn
- 2013-05-29 EP EP17200169.5A patent/EP3327113B1/en active Active
- 2013-05-29 BR BR112014029252A patent/BR112014029252B8/pt active Search and Examination
- 2013-05-29 WO PCT/EP2013/061052 patent/WO2013178674A1/en not_active Ceased
- 2013-05-29 US US14/403,355 patent/US9487767B2/en active Active
-
2016
- 2016-10-18 US US15/296,433 patent/US10351861B2/en active Active
-
2018
- 2018-06-26 US US16/018,726 patent/US10351863B2/en active Active
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