JP6449763B2 - 真菌における改善された選択 - Google Patents

真菌における改善された選択 Download PDF

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JP6449763B2
JP6449763B2 JP2015514483A JP2015514483A JP6449763B2 JP 6449763 B2 JP6449763 B2 JP 6449763B2 JP 2015514483 A JP2015514483 A JP 2015514483A JP 2015514483 A JP2015514483 A JP 2015514483A JP 6449763 B2 JP6449763 B2 JP 6449763B2
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seq
polynucleotide
host cell
intron
fungal host
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JP2015517816A (ja
JP2015517816A5 (https=
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リレルンド オルセン カーステン
リレルンド オルセン カーステン
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ノボザイムス アクティーゼルスカブ
ノボザイムス アクティーゼルスカブ
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01003—Nitrate reductase (NADPH) (1.7.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01004—Nitrite reductase [NAD(P)H] (1.7.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01011—Phosphopyruvate hydratase (4.2.1.11), i.e. enolase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2015514483A 2012-05-31 2013-05-29 真菌における改善された選択 Active JP6449763B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12170260.9 2012-05-31
EP12170260 2012-05-31
PCT/EP2013/061052 WO2013178674A1 (en) 2012-05-31 2013-05-29 Improved selection in fungi

Publications (3)

Publication Number Publication Date
JP2015517816A JP2015517816A (ja) 2015-06-25
JP2015517816A5 JP2015517816A5 (https=) 2016-07-21
JP6449763B2 true JP6449763B2 (ja) 2019-01-09

Family

ID=48539153

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JP2015514483A Active JP6449763B2 (ja) 2012-05-31 2013-05-29 真菌における改善された選択

Country Status (7)

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US (3) US9487767B2 (https=)
EP (2) EP3327113B1 (https=)
JP (1) JP6449763B2 (https=)
CN (1) CN104395454B (https=)
BR (1) BR112014029252B8 (https=)
DK (1) DK3327113T3 (https=)
WO (1) WO2013178674A1 (https=)

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EP3113609B1 (en) 2014-03-05 2019-07-31 Novozymes A/S Methods for improving post-harvest properties of agricultural crops
US20170073890A1 (en) 2014-03-05 2017-03-16 Novozymes A/S Compositions and Methods for Functionalizing and Linking Materials
EP3114272A1 (en) 2014-03-05 2017-01-11 Novozymes A/S Compositions and methods for improving properties of cellulosic textile materials with xyloglucan endotransglycosylase
US20160348035A1 (en) 2014-03-05 2016-12-01 Novozymes A/S Compositions and Methods for Improving Properties of Non-Cellulosic Textile Materials with Xyloglucan Endotransglycosylase
BR112016020374A2 (pt) 2014-03-05 2018-01-23 Novozymes As formulação, e, métodos para potenciar o crescimento de plantas e para formular um ou mais agentes agricolamente benéficos
EP3183339A1 (en) 2014-08-20 2017-06-28 Novozymes A/S Xyloglucan endotransglycosylase variants and polynucleotides encoding same
EP3227438B1 (en) 2014-12-02 2024-03-27 Novozymes A/S Laccase variants and polynucleotides encoding same
PL3265563T3 (pl) 2015-02-02 2021-09-13 Meiragtx Uk Ii Limited Regulacja ekspresji genów poprzez modulację alternatywnego splicingu za pośrednictwem aptamerów
CN107429220A (zh) * 2015-03-27 2017-12-01 嘉吉公司 经葡糖淀粉酶修饰的酵母菌株和用于产生生物产物的方法
WO2016174258A1 (en) 2015-04-29 2016-11-03 Boehringer Ingelheim Rcv Gmbh & Co Kg Means and methods for protein production
BR112018011902A2 (pt) 2015-12-17 2018-12-04 Cargill Inc método de fermentação, levedura geneticamente modificada, construto de ácido nucleico, vetor, célula hospedeira, meio de fermentação e utilização da levedura geneticamente modificada
SMT202400091T1 (it) * 2016-08-03 2024-05-14 Meiragtx Uk Ii Ltd Screening ad alta produzione basato su cellule per 5 aptameri
WO2018027131A1 (en) 2016-08-05 2018-02-08 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production
CN108949579B (zh) * 2018-04-19 2021-10-01 中国科学技术大学 嗜热子囊菌基因表达系统
JP7210577B2 (ja) * 2018-06-15 2023-01-23 キッコーマン株式会社 セレノネインの製造方法
US20210301285A1 (en) 2018-08-02 2021-09-30 Novozymes A/S Preparation of Combinatorial Libraries of DNA Constructs
DK3898985T5 (da) 2018-12-21 2024-08-05 Novozymes As Proteinekspression i tandem
CN110592149B (zh) * 2019-07-16 2020-07-14 甘肃省科学院生物研究所 一种梨囊鞭菌发酵秸秆生产乳酸的新方法和应用
EP4004211A1 (en) 2019-07-25 2022-06-01 Novozymes A/S Filamentous fungal expression system
US11479779B2 (en) * 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
CN114292761B (zh) * 2021-12-02 2023-11-03 南京工业大学 一株黑曲霉基因工程菌及构建方法与应用
WO2025133003A2 (en) 2023-12-19 2025-06-26 21St.Bio A/S Non-animal bovine beta lactoglobulin
CN119752977A (zh) * 2023-12-28 2025-04-04 南京百斯杰生物工程有限公司 可改善目标多肽表达效果的多核苷酸构建体及其应用
WO2025226596A1 (en) 2024-04-24 2025-10-30 Novozymes A/S Methods for producing secreted polypeptides

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DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
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ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
AU4424597A (en) 1996-09-13 1998-04-02 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide
DK1124949T3 (da) 1998-10-26 2006-11-06 Novozymes As Konstruktion og screening af et DNA-bibliotek af interesse i trådformede svampeceller
CN100482801C (zh) 1999-03-22 2009-04-29 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
WO2001090393A1 (en) 2000-05-24 2001-11-29 Novozymes A/S Method for increasing gene copy number in a host cell and resulting host cell
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Also Published As

Publication number Publication date
US20180298394A1 (en) 2018-10-18
BR112014029252B1 (pt) 2021-10-19
US20170029830A1 (en) 2017-02-02
US10351863B2 (en) 2019-07-16
CN104395454A (zh) 2015-03-04
BR112014029252B8 (pt) 2022-01-25
BR112014029252A2 (pt) 2017-07-18
WO2013178674A1 (en) 2013-12-05
EP3327113B1 (en) 2021-08-18
EP3327113A3 (en) 2018-08-08
US20150175981A1 (en) 2015-06-25
JP2015517816A (ja) 2015-06-25
US9487767B2 (en) 2016-11-08
EP3327113A2 (en) 2018-05-30
CN104395454B (zh) 2021-11-30
EP2855659A1 (en) 2015-04-08
DK3327113T3 (da) 2021-09-13
US10351861B2 (en) 2019-07-16

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