JP2015505062A5 - - Google Patents
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- JP2015505062A5 JP2015505062A5 JP2014554873A JP2014554873A JP2015505062A5 JP 2015505062 A5 JP2015505062 A5 JP 2015505062A5 JP 2014554873 A JP2014554873 A JP 2014554873A JP 2014554873 A JP2014554873 A JP 2014554873A JP 2015505062 A5 JP2015505062 A5 JP 2015505062A5
- Authority
- JP
- Japan
- Prior art keywords
- buffer
- staining
- acid
- solution
- specimen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 claims description 16
- 238000000034 method Methods 0.000 claims 18
- 239000007853 buffer solution Substances 0.000 claims 7
- 239000006174 pH buffer Substances 0.000 claims 7
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims 6
- 150000007524 organic acids Chemical class 0.000 claims 5
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims 4
- 238000010186 staining Methods 0.000 claims 4
- 239000011975 tartaric acid Substances 0.000 claims 4
- 235000002906 tartaric acid Nutrition 0.000 claims 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims 3
- 239000012192 staining solution Substances 0.000 claims 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 3
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims 2
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 2
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims 2
- 238000004043 dyeing Methods 0.000 claims 2
- 239000001630 malic acid Substances 0.000 claims 2
- 235000011090 malic acid Nutrition 0.000 claims 2
- 239000000243 solution Substances 0.000 claims 2
- 150000005846 sugar alcohols Polymers 0.000 claims 2
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims 1
- 239000005977 Ethylene Substances 0.000 claims 1
- 239000002202 Polyethylene glycol Substances 0.000 claims 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims 1
- 230000002411 adverse Effects 0.000 claims 1
- 239000004599 antimicrobial Substances 0.000 claims 1
- 235000015165 citric acid Nutrition 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 235000011187 glycerol Nutrition 0.000 claims 1
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 claims 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 claims 1
- 239000011976 maleic acid Substances 0.000 claims 1
- 229920001223 polyethylene glycol Polymers 0.000 claims 1
- 239000012128 staining reagent Substances 0.000 claims 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000000872 buffer Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261591181P | 2012-01-26 | 2012-01-26 | |
| US61/591,181 | 2012-01-26 | ||
| PCT/US2013/023226 WO2013112891A1 (en) | 2012-01-26 | 2013-01-25 | Methods and compositions for hematoxylin and eosin staining |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016163326A Division JP6351670B2 (ja) | 2012-01-26 | 2016-08-24 | ヘマトキシリン及びエオシン染色のための方法及び組成物 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015505062A JP2015505062A (ja) | 2015-02-16 |
| JP2015505062A5 true JP2015505062A5 (https=) | 2016-08-18 |
| JP5996675B2 JP5996675B2 (ja) | 2016-09-21 |
Family
ID=47664460
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014554873A Active JP5996675B2 (ja) | 2012-01-26 | 2013-01-25 | ヘマトキシリン及びエオシン染色のための方法及び組成物 |
| JP2016163326A Active JP6351670B2 (ja) | 2012-01-26 | 2016-08-24 | ヘマトキシリン及びエオシン染色のための方法及び組成物 |
| JP2018025085A Pending JP2018109637A (ja) | 2012-01-26 | 2018-02-15 | ヘマトキシリン及びエオシン染色のための方法及び組成物 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016163326A Active JP6351670B2 (ja) | 2012-01-26 | 2016-08-24 | ヘマトキシリン及びエオシン染色のための方法及び組成物 |
| JP2018025085A Pending JP2018109637A (ja) | 2012-01-26 | 2018-02-15 | ヘマトキシリン及びエオシン染色のための方法及び組成物 |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US20130203109A1 (https=) |
| EP (2) | EP3264095B1 (https=) |
| JP (3) | JP5996675B2 (https=) |
| CN (2) | CN106872715B (https=) |
| ES (1) | ES2827785T3 (https=) |
| WO (1) | WO2013112891A1 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2827785T3 (es) | 2012-01-26 | 2021-05-24 | Leica Biosystems Richmond Inc | Kit para tinción con hematoxilina y eosina |
| WO2015086485A1 (en) | 2013-12-13 | 2015-06-18 | Ventana Medical Systems, Inc. | Thermal management in the context of automated histological processing of biological specimens and associated technology |
| CN105980828B (zh) | 2013-12-13 | 2021-12-17 | 文塔纳医疗系统公司 | 用于生物标本组织处理的染色试剂和其它液体及关联技术 |
| EP3298381B1 (en) | 2015-05-22 | 2023-01-25 | Ventana Medical Systems, Inc. | METHOD AND APPARATUS FOR THE REMOVAL OR REDUCTION OF 
PRECIPITATES FORMED IN HEMATOXYLIN SOLUTIONS |
| CN105651580B (zh) * | 2015-12-28 | 2019-01-29 | 北京雷根生物技术有限公司 | 一种苏木素伊红混合染色液 |
| CN105987841B (zh) * | 2016-06-30 | 2018-10-09 | 赖保思 | 伊红染色液 |
| CN106644656B (zh) * | 2017-01-06 | 2019-01-22 | 杨海玉 | 苏木素-伊红一步染色法 |
| EP3622266B1 (en) | 2017-05-10 | 2022-08-17 | Ventana Medical Systems, Inc. | Stabilized two-part hematoxylin solution utilizing ph adjustment, method for staining and kit |
| EP3729047A2 (en) | 2017-12-24 | 2020-10-28 | Ventana Medical Systems, Inc. | Eosin staining techniques |
| CN109813588B (zh) * | 2019-02-19 | 2021-03-02 | 湖北泰康医疗设备有限公司 | 一种无需固定的寄生虫染色液 |
| CN110823666A (zh) * | 2019-11-27 | 2020-02-21 | 李雄 | 苏木素在制备减少病理常规切片染色后褪色的染色剂中的应用 |
| CN113790945B (zh) * | 2021-09-23 | 2024-02-20 | 南昌雨露实验器材有限公司 | 一种改良的苏木精染液及其制备方法 |
| US20250044295A1 (en) * | 2021-12-16 | 2025-02-06 | Merck Sharp & Dohme Llc | Biomarkers for predicting eligibility for an anti-ilt4 and anti-pd-1 combination therapy |
| CN114624084A (zh) * | 2022-03-18 | 2022-06-14 | 深圳市贝安特医疗技术有限公司 | 一种病理组织切片染色试剂盒的制备、染色方法 |
Family Cites Families (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54134694A (en) * | 1978-04-12 | 1979-10-19 | Chemo Sero Therapeut Res Inst | Method and kit for measuring uric acid |
| JPS58179499A (ja) | 1982-04-13 | 1983-10-20 | Wako Pure Chem Ind Ltd | 細菌感受性試験ディスク |
| JPH0659231B2 (ja) * | 1983-02-08 | 1994-08-10 | ヤマサ醤油株式会社 | 単一クローン抗体 |
| CN88103080A (zh) * | 1987-05-25 | 1988-12-14 | 奥里尔股份有限公司 | 成色剂和/或“快速”氧化染色剂与碘化物并用进行角质纤维染色的方法及其中所用染色组合物 |
| JP2702211B2 (ja) * | 1989-02-03 | 1998-01-21 | サクラ精機株式会社 | ヘマトキシリン染色液 |
| WO1992019952A1 (en) | 1991-05-07 | 1992-11-12 | Wescor, Inc. | Improved staining method for histology and cytology specimens |
| JPH05252954A (ja) * | 1991-08-06 | 1993-10-05 | Chemo Sero Therapeut Res Inst | 新規な組織因子インヒビターをコードする遺伝子断片 |
| US5296090A (en) * | 1991-12-03 | 1994-03-22 | New England Medical Center Hospitals, Inc. | High resolution track etch autoradiography |
| US5295998A (en) | 1993-02-02 | 1994-03-22 | Liquid Carbonic Industries Corporation | Adjusting pH in dyeing processes using CO2 |
| US5432056A (en) * | 1993-11-15 | 1995-07-11 | Ventana Medical Systems, Inc. | Bisulfite-based tissue fixative |
| US6735531B2 (en) | 1996-10-07 | 2004-05-11 | Lab Vision Corporation | Method and apparatus for automatic tissue staining |
| JP3847559B2 (ja) | 1998-02-27 | 2006-11-22 | ベンタナ・メデイカル・システムズ・インコーポレーテツド | 独立スライド加熱器を有する自動化分子病理学装置 |
| US7410753B2 (en) | 1998-09-03 | 2008-08-12 | Ventana Medical Systems, Inc. | Removal of embedding media from biological samples and cell conditioning on automated staining instruments |
| AU766614B2 (en) * | 1998-09-03 | 2003-10-23 | Ventana Medical Systems, Inc. | Removal of embedding media from biological samples and cell conditioning on automated staining instruments |
| US6855552B2 (en) * | 1998-09-03 | 2005-02-15 | Ventana Medical Systems | Automated immunohistochemical and in situ hybridization assay formulations |
| JP2000187033A (ja) * | 1998-12-21 | 2000-07-04 | Wako Pure Chem Ind Ltd | 細胞染色液 |
| US6544798B1 (en) * | 1999-02-26 | 2003-04-08 | Ventana Medical Systems, Inc. | Removal of embedding media from biological samples and cell conditioning on automated staining instruments |
| US6348325B1 (en) | 1999-10-29 | 2002-02-19 | Cytyc Corporation | Cytological stain composition |
| US6593102B2 (en) | 1999-10-29 | 2003-07-15 | Cytyc Corporation | Cytological stain composition |
| US20030100043A1 (en) | 2000-03-24 | 2003-05-29 | Biogenex Laboratories, Inc. | Device and methods for automated specimen processing |
| EP1417632A1 (en) | 2001-05-29 | 2004-05-12 | Tissueinformatics, Inc. | Robust stain detection and quantification for histological specimens based on a physical model for stain absorption |
| US7468161B2 (en) | 2002-04-15 | 2008-12-23 | Ventana Medical Systems, Inc. | Automated high volume slide processing system |
| WO2003089140A1 (en) | 2002-04-15 | 2003-10-30 | Ventana Medical Systems, Inc. | Automated high volume slide staining system |
| KR20050083623A (ko) | 2002-12-10 | 2005-08-26 | 마쯔시다덴기산교 가부시키가이샤 | 면역반응 측정방법 |
| US7875245B2 (en) | 2003-05-14 | 2011-01-25 | Dako Denmark A/S | Method and apparatus for automated pre-treatment and processing of biological samples |
| EP1573298B1 (en) | 2002-12-20 | 2012-08-08 | Dako Denmark A/S | An automated sample processing apparatus and a method of automated treating of samples and use of such apparatus |
| US7186522B2 (en) * | 2003-03-31 | 2007-03-06 | Cytyc Corporation | Papanicolau staining process |
| ES2381644T3 (es) * | 2004-03-24 | 2012-05-30 | Tripath Imaging, Inc. | Métodos y composiciones para la detección de enfermedades de cuello uterino |
| WO2007029437A1 (ja) * | 2005-09-06 | 2007-03-15 | Pola Chemical Industries Inc. | 角層細胞の染色液及びそれを用いた角層細胞の染色方法 |
| JP2007114076A (ja) * | 2005-10-21 | 2007-05-10 | Pola Chem Ind Inc | 角層細胞の画像の調整方法 |
| US8189737B2 (en) * | 2006-01-26 | 2012-05-29 | The Board Of Regents, The University Of Texas System, A Texas Institution Of Higher Learning | Process and apparatus for microCT imaging of ex vivo specimens |
| US7915432B2 (en) * | 2006-12-12 | 2011-03-29 | Cytyc Corporation | Method for improving the shelf-life of hematoxylin staining solutions |
| WO2008112993A1 (en) * | 2007-03-15 | 2008-09-18 | Ventana Medical Systems, Inc. | Stabilized hematoxylin |
| KR101244501B1 (ko) * | 2007-09-27 | 2013-03-18 | 시스멕스 가부시키가이샤 | 시료 분석용 시약 키트 및 시료 분석 방법 |
| US8158381B2 (en) | 2008-03-27 | 2012-04-17 | Richard-Allan Scientific Company | Methods for integrated tissue processing and staining |
| WO2009148885A2 (en) * | 2008-05-30 | 2009-12-10 | Ventana Medical Systems, Inc. | Hematoxylin staining method to address gradient staining |
| JP5683501B2 (ja) | 2010-02-05 | 2015-03-11 | 株式会社ニチレイバイオサイエンス | 免疫組織化学染色用前処理液及びその濃縮液 |
| WO2011157678A1 (en) * | 2010-06-14 | 2011-12-22 | Qiagen Gmbh | Method for determination of target cells or tissue for extraction of biomolecules from fixed biological samples |
| ES2827785T3 (es) | 2012-01-26 | 2021-05-24 | Leica Biosystems Richmond Inc | Kit para tinción con hematoxilina y eosina |
-
2013
- 2013-01-25 ES ES17181088T patent/ES2827785T3/es active Active
- 2013-01-25 EP EP17181088.0A patent/EP3264095B1/en active Active
- 2013-01-25 US US13/750,686 patent/US20130203109A1/en not_active Abandoned
- 2013-01-25 CN CN201710117348.2A patent/CN106872715B/zh active Active
- 2013-01-25 JP JP2014554873A patent/JP5996675B2/ja active Active
- 2013-01-25 EP EP13702719.9A patent/EP2807490B1/en active Active
- 2013-01-25 WO PCT/US2013/023226 patent/WO2013112891A1/en not_active Ceased
- 2013-01-25 CN CN201380016665.5A patent/CN104471405B/zh active Active
-
2014
- 2014-05-02 US US14/268,274 patent/US9423322B2/en active Active
-
2016
- 2016-07-22 US US15/216,910 patent/US10234365B2/en active Active
- 2016-08-24 JP JP2016163326A patent/JP6351670B2/ja active Active
-
2018
- 2018-02-15 JP JP2018025085A patent/JP2018109637A/ja active Pending
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