JP5996675B2 - ヘマトキシリン及びエオシン染色のための方法及び組成物 - Google Patents

ヘマトキシリン及びエオシン染色のための方法及び組成物 Download PDF

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JP5996675B2
JP5996675B2 JP2014554873A JP2014554873A JP5996675B2 JP 5996675 B2 JP5996675 B2 JP 5996675B2 JP 2014554873 A JP2014554873 A JP 2014554873A JP 2014554873 A JP2014554873 A JP 2014554873A JP 5996675 B2 JP5996675 B2 JP 5996675B2
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solution
staining
hematoxylin
buffer
acid
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JP2015505062A (ja
JP2015505062A5 (https=
Inventor
イー． ハンセン スタンリー
イー． ハンセン スタンリー
マイアーズ ラッセル
マイアーズ ラッセル
ポール ロナルド
ポール ロナルド
ラスムッセン オードラ
ラスムッセン オードラ
テイラー ケイト
テイラー ケイト
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Leica Biosystems Richmond Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • G01N33/6839—Total protein determination, e.g. albumin in urine involving dyes, e.g. Coomassie blue, bromcresol green
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/302—Stain compositions

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2014554873A 2012-01-26 2013-01-25 ヘマトキシリン及びエオシン染色のための方法及び組成物 Active JP5996675B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261591181P 2012-01-26 2012-01-26
US61/591,181 2012-01-26
PCT/US2013/023226 WO2013112891A1 (en) 2012-01-26 2013-01-25 Methods and compositions for hematoxylin and eosin staining

Related Child Applications (1)

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JP2016163326A Division JP6351670B2 (ja) 2012-01-26 2016-08-24 ヘマトキシリン及びエオシン染色のための方法及び組成物

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JP2015505062A JP2015505062A (ja) 2015-02-16
JP2015505062A5 JP2015505062A5 (https=) 2016-08-18
JP5996675B2 true JP5996675B2 (ja) 2016-09-21

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JP2014554873A Active JP5996675B2 (ja) 2012-01-26 2013-01-25 ヘマトキシリン及びエオシン染色のための方法及び組成物
JP2016163326A Active JP6351670B2 (ja) 2012-01-26 2016-08-24 ヘマトキシリン及びエオシン染色のための方法及び組成物
JP2018025085A Pending JP2018109637A (ja) 2012-01-26 2018-02-15 ヘマトキシリン及びエオシン染色のための方法及び組成物

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JP2016163326A Active JP6351670B2 (ja) 2012-01-26 2016-08-24 ヘマトキシリン及びエオシン染色のための方法及び組成物
JP2018025085A Pending JP2018109637A (ja) 2012-01-26 2018-02-15 ヘマトキシリン及びエオシン染色のための方法及び組成物

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US (3) US20130203109A1 (https=)
EP (2) EP3264095B1 (https=)
JP (3) JP5996675B2 (https=)
CN (2) CN106872715B (https=)
ES (1) ES2827785T3 (https=)
WO (1) WO2013112891A1 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2827785T3 (es) 2012-01-26 2021-05-24 Leica Biosystems Richmond Inc Kit para tinción con hematoxilina y eosina
WO2015086485A1 (en) 2013-12-13 2015-06-18 Ventana Medical Systems, Inc. Thermal management in the context of automated histological processing of biological specimens and associated technology
CN105980828B (zh) 2013-12-13 2021-12-17 文塔纳医疗系统公司 用于生物标本组织处理的染色试剂和其它液体及关联技术
EP3298381B1 (en) 2015-05-22 2023-01-25 Ventana Medical Systems, Inc. METHOD AND APPARATUS FOR THE REMOVAL OR REDUCTION OF 
PRECIPITATES FORMED IN HEMATOXYLIN SOLUTIONS
CN105651580B (zh) * 2015-12-28 2019-01-29 北京雷根生物技术有限公司 一种苏木素伊红混合染色液
CN105987841B (zh) * 2016-06-30 2018-10-09 赖保思 伊红染色液
CN106644656B (zh) * 2017-01-06 2019-01-22 杨海玉 苏木素-伊红一步染色法
EP3622266B1 (en) 2017-05-10 2022-08-17 Ventana Medical Systems, Inc. Stabilized two-part hematoxylin solution utilizing ph adjustment, method for staining and kit
EP3729047A2 (en) 2017-12-24 2020-10-28 Ventana Medical Systems, Inc. Eosin staining techniques
CN109813588B (zh) * 2019-02-19 2021-03-02 湖北泰康医疗设备有限公司 一种无需固定的寄生虫染色液
CN110823666A (zh) * 2019-11-27 2020-02-21 李雄 苏木素在制备减少病理常规切片染色后褪色的染色剂中的应用
CN113790945B (zh) * 2021-09-23 2024-02-20 南昌雨露实验器材有限公司 一种改良的苏木精染液及其制备方法
US20250044295A1 (en) * 2021-12-16 2025-02-06 Merck Sharp & Dohme Llc Biomarkers for predicting eligibility for an anti-ilt4 and anti-pd-1 combination therapy
CN114624084A (zh) * 2022-03-18 2022-06-14 深圳市贝安特医疗技术有限公司 一种病理组织切片染色试剂盒的制备、染色方法

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54134694A (en) * 1978-04-12 1979-10-19 Chemo Sero Therapeut Res Inst Method and kit for measuring uric acid
JPS58179499A (ja) 1982-04-13 1983-10-20 Wako Pure Chem Ind Ltd 細菌感受性試験ディスク
JPH0659231B2 (ja) * 1983-02-08 1994-08-10 ヤマサ醤油株式会社 単一クローン抗体
CN88103080A (zh) * 1987-05-25 1988-12-14 奥里尔股份有限公司 成色剂和/或“快速”氧化染色剂与碘化物并用进行角质纤维染色的方法及其中所用染色组合物
JP2702211B2 (ja) * 1989-02-03 1998-01-21 サクラ精機株式会社 ヘマトキシリン染色液
WO1992019952A1 (en) 1991-05-07 1992-11-12 Wescor, Inc. Improved staining method for histology and cytology specimens
JPH05252954A (ja) * 1991-08-06 1993-10-05 Chemo Sero Therapeut Res Inst 新規な組織因子インヒビターをコードする遺伝子断片
US5296090A (en) * 1991-12-03 1994-03-22 New England Medical Center Hospitals, Inc. High resolution track etch autoradiography
US5295998A (en) 1993-02-02 1994-03-22 Liquid Carbonic Industries Corporation Adjusting pH in dyeing processes using CO2
US5432056A (en) * 1993-11-15 1995-07-11 Ventana Medical Systems, Inc. Bisulfite-based tissue fixative
US6735531B2 (en) 1996-10-07 2004-05-11 Lab Vision Corporation Method and apparatus for automatic tissue staining
JP3847559B2 (ja) 1998-02-27 2006-11-22 ベンタナ・メデイカル・システムズ・インコーポレーテツド 独立スライド加熱器を有する自動化分子病理学装置
US7410753B2 (en) 1998-09-03 2008-08-12 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
AU766614B2 (en) * 1998-09-03 2003-10-23 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6855552B2 (en) * 1998-09-03 2005-02-15 Ventana Medical Systems Automated immunohistochemical and in situ hybridization assay formulations
JP2000187033A (ja) * 1998-12-21 2000-07-04 Wako Pure Chem Ind Ltd 細胞染色液
US6544798B1 (en) * 1999-02-26 2003-04-08 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6348325B1 (en) 1999-10-29 2002-02-19 Cytyc Corporation Cytological stain composition
US6593102B2 (en) 1999-10-29 2003-07-15 Cytyc Corporation Cytological stain composition
US20030100043A1 (en) 2000-03-24 2003-05-29 Biogenex Laboratories, Inc. Device and methods for automated specimen processing
EP1417632A1 (en) 2001-05-29 2004-05-12 Tissueinformatics, Inc. Robust stain detection and quantification for histological specimens based on a physical model for stain absorption
US7468161B2 (en) 2002-04-15 2008-12-23 Ventana Medical Systems, Inc. Automated high volume slide processing system
WO2003089140A1 (en) 2002-04-15 2003-10-30 Ventana Medical Systems, Inc. Automated high volume slide staining system
KR20050083623A (ko) 2002-12-10 2005-08-26 마쯔시다덴기산교 가부시키가이샤 면역반응 측정방법
US7875245B2 (en) 2003-05-14 2011-01-25 Dako Denmark A/S Method and apparatus for automated pre-treatment and processing of biological samples
EP1573298B1 (en) 2002-12-20 2012-08-08 Dako Denmark A/S An automated sample processing apparatus and a method of automated treating of samples and use of such apparatus
US7186522B2 (en) * 2003-03-31 2007-03-06 Cytyc Corporation Papanicolau staining process
ES2381644T3 (es) * 2004-03-24 2012-05-30 Tripath Imaging, Inc. Métodos y composiciones para la detección de enfermedades de cuello uterino
WO2007029437A1 (ja) * 2005-09-06 2007-03-15 Pola Chemical Industries Inc. 角層細胞の染色液及びそれを用いた角層細胞の染色方法
JP2007114076A (ja) * 2005-10-21 2007-05-10 Pola Chem Ind Inc 角層細胞の画像の調整方法
US8189737B2 (en) * 2006-01-26 2012-05-29 The Board Of Regents, The University Of Texas System, A Texas Institution Of Higher Learning Process and apparatus for microCT imaging of ex vivo specimens
US7915432B2 (en) * 2006-12-12 2011-03-29 Cytyc Corporation Method for improving the shelf-life of hematoxylin staining solutions
WO2008112993A1 (en) * 2007-03-15 2008-09-18 Ventana Medical Systems, Inc. Stabilized hematoxylin
KR101244501B1 (ko) * 2007-09-27 2013-03-18 시스멕스 가부시키가이샤 시료 분석용 시약 키트 및 시료 분석 방법
US8158381B2 (en) 2008-03-27 2012-04-17 Richard-Allan Scientific Company Methods for integrated tissue processing and staining
WO2009148885A2 (en) * 2008-05-30 2009-12-10 Ventana Medical Systems, Inc. Hematoxylin staining method to address gradient staining
JP5683501B2 (ja) 2010-02-05 2015-03-11 株式会社ニチレイバイオサイエンス 免疫組織化学染色用前処理液及びその濃縮液
WO2011157678A1 (en) * 2010-06-14 2011-12-22 Qiagen Gmbh Method for determination of target cells or tissue for extraction of biomolecules from fixed biological samples
ES2827785T3 (es) 2012-01-26 2021-05-24 Leica Biosystems Richmond Inc Kit para tinción con hematoxilina y eosina

Also Published As

Publication number Publication date
US20130203109A1 (en) 2013-08-08
JP6351670B2 (ja) 2018-07-04
CN104471405A (zh) 2015-03-25
WO2013112891A1 (en) 2013-08-01
CN104471405B (zh) 2017-03-29
US20160327458A1 (en) 2016-11-10
JP2018109637A (ja) 2018-07-12
JP2016218081A (ja) 2016-12-22
CN106872715A (zh) 2017-06-20
US9423322B2 (en) 2016-08-23
US20140242634A1 (en) 2014-08-28
US10234365B2 (en) 2019-03-19
ES2827785T3 (es) 2021-05-24
JP2015505062A (ja) 2015-02-16
CN106872715B (zh) 2020-06-16
EP2807490A1 (en) 2014-12-03
EP2807490B1 (en) 2017-09-13
EP3264095A1 (en) 2018-01-03
EP3264095B1 (en) 2020-08-12

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