JP2013536696A5 - - Google Patents
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- Publication number
- JP2013536696A5 JP2013536696A5 JP2013527735A JP2013527735A JP2013536696A5 JP 2013536696 A5 JP2013536696 A5 JP 2013536696A5 JP 2013527735 A JP2013527735 A JP 2013527735A JP 2013527735 A JP2013527735 A JP 2013527735A JP 2013536696 A5 JP2013536696 A5 JP 2013536696A5
- Authority
- JP
- Japan
- Prior art keywords
- pluripotent stem
- stem cells
- culture
- cells
- aggregates
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 35
- 238000000034 method Methods 0.000 claims 22
- 210000001671 embryonic stem cell Anatomy 0.000 claims 11
- 210000004027 cell Anatomy 0.000 claims 10
- 239000001963 growth medium Substances 0.000 claims 10
- 238000004114 suspension culture Methods 0.000 claims 10
- 238000012258 culturing Methods 0.000 claims 7
- 102000003815 Interleukin-11 Human genes 0.000 claims 5
- 108090000177 Interleukin-11 Proteins 0.000 claims 5
- 238000010494 dissociation reaction Methods 0.000 claims 5
- 230000005593 dissociations Effects 0.000 claims 5
- 210000002242 embryoid body Anatomy 0.000 claims 5
- 229940074383 interleukin-11 Drugs 0.000 claims 5
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 claims 4
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims 4
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims 2
- 108010005939 Ciliary Neurotrophic Factor Proteins 0.000 claims 2
- 102100031614 Ciliary neurotrophic factor Human genes 0.000 claims 2
- 210000002459 blastocyst Anatomy 0.000 claims 2
- 238000010367 cloning Methods 0.000 claims 2
- 230000004069 differentiation Effects 0.000 claims 2
- 210000003981 ectoderm Anatomy 0.000 claims 2
- 210000001654 germ layer Anatomy 0.000 claims 2
- 210000003716 mesoderm Anatomy 0.000 claims 2
- 102000000568 rho-Associated Kinases Human genes 0.000 claims 2
- 108010041788 rho-Associated Kinases Proteins 0.000 claims 2
- 241001465754 Metazoa Species 0.000 claims 1
- 102000004887 Transforming Growth Factor beta Human genes 0.000 claims 1
- 108090001012 Transforming Growth Factor beta Proteins 0.000 claims 1
- 229960005070 ascorbic acid Drugs 0.000 claims 1
- 235000010323 ascorbic acid Nutrition 0.000 claims 1
- 239000011668 ascorbic acid Substances 0.000 claims 1
- 238000004113 cell culture Methods 0.000 claims 1
- 239000000356 contaminant Substances 0.000 claims 1
- 210000001900 endoderm Anatomy 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 230000001605 fetal effect Effects 0.000 claims 1
- 210000002950 fibroblast Anatomy 0.000 claims 1
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims 1
- 239000003112 inhibitor Substances 0.000 claims 1
- 210000001161 mammalian embryo Anatomy 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 230000001850 reproductive effect Effects 0.000 claims 1
- 210000002966 serum Anatomy 0.000 claims 1
- 210000000130 stem cell Anatomy 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US38038810P | 2010-09-07 | 2010-09-07 | |
| US61/380,388 | 2010-09-07 | ||
| PCT/IL2011/000722 WO2012032521A2 (en) | 2010-09-07 | 2011-09-07 | Novel methods and culture media for culturing pluripotent stem cells |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017113227A Division JP6574458B2 (ja) | 2010-09-07 | 2017-06-08 | 多能性幹細胞を培養するための新規な方法および培養培地 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013536696A JP2013536696A (ja) | 2013-09-26 |
| JP2013536696A5 true JP2013536696A5 (enExample) | 2014-10-23 |
| JP6283223B2 JP6283223B2 (ja) | 2018-02-21 |
Family
ID=44936324
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013527735A Active JP6283223B2 (ja) | 2010-09-07 | 2011-09-07 | 多能性幹細胞を培養するための新規な方法および培養培地 |
| JP2017113227A Active JP6574458B2 (ja) | 2010-09-07 | 2017-06-08 | 多能性幹細胞を培養するための新規な方法および培養培地 |
| JP2019149488A Pending JP2020000241A (ja) | 2010-09-07 | 2019-08-16 | 多能性幹細胞を培養するための新規な方法および培養培地 |
| JP2022007129A Active JP7357086B2 (ja) | 2010-09-07 | 2022-01-20 | 多能性幹細胞を培養するための新規な方法および培養培地 |
| JP2023161645A Active JP7663644B2 (ja) | 2010-09-07 | 2023-09-25 | 多能性幹細胞を培養するための新規な方法および培養培地 |
Family Applications After (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017113227A Active JP6574458B2 (ja) | 2010-09-07 | 2017-06-08 | 多能性幹細胞を培養するための新規な方法および培養培地 |
| JP2019149488A Pending JP2020000241A (ja) | 2010-09-07 | 2019-08-16 | 多能性幹細胞を培養するための新規な方法および培養培地 |
| JP2022007129A Active JP7357086B2 (ja) | 2010-09-07 | 2022-01-20 | 多能性幹細胞を培養するための新規な方法および培養培地 |
| JP2023161645A Active JP7663644B2 (ja) | 2010-09-07 | 2023-09-25 | 多能性幹細胞を培養するための新規な方法および培養培地 |
Country Status (9)
| Country | Link |
|---|---|
| US (5) | US10214722B2 (enExample) |
| EP (2) | EP3572501B1 (enExample) |
| JP (5) | JP6283223B2 (enExample) |
| CA (2) | CA3150342A1 (enExample) |
| DK (1) | DK2614141T3 (enExample) |
| ES (1) | ES2746482T3 (enExample) |
| IL (4) | IL225070B (enExample) |
| SG (4) | SG188411A1 (enExample) |
| WO (1) | WO2012032521A2 (enExample) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3712258B1 (en) | 2006-04-14 | 2025-01-01 | Astellas Institute for Regenerative Medicine | Hemangio-colony forming cells |
| EP2499236B1 (en) | 2009-11-12 | 2020-01-01 | Technion Research & Development Foundation Ltd. | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
| US10214722B2 (en) | 2010-09-07 | 2019-02-26 | Technion Research & Development Foundation Limited | Methods for expanding and maintaining human pluripotent stem cells (PSCs) in an undifferentiated state in a single cell suspension culture |
| JP5745919B2 (ja) * | 2011-04-28 | 2015-07-08 | 浜松ホトニクス株式会社 | 細胞解析方法、細胞解析装置、および細胞解析プログラム |
| US9175263B2 (en) * | 2012-08-22 | 2015-11-03 | Biotime, Inc. | Methods and compositions for targeting progenitor cell lines |
| EP3003290B1 (en) | 2013-06-05 | 2021-03-10 | AgeX Therapeutics, Inc. | Compositions for use in the treatment of wounds in mammalian species |
| US11078462B2 (en) | 2014-02-18 | 2021-08-03 | ReCyte Therapeutics, Inc. | Perivascular stromal cells from primate pluripotent stem cells |
| WO2015181253A1 (en) * | 2014-05-27 | 2015-12-03 | Fundación Pública Andaluza Progreso Y Salud | Neural progenitor cell population |
| US10240127B2 (en) | 2014-07-03 | 2019-03-26 | ReCyte Therapeutics, Inc. | Exosomes from clonal progenitor cells |
| US9944894B2 (en) | 2015-01-16 | 2018-04-17 | General Electric Company | Pluripotent stem cell expansion and passage using a rocking platform bioreactor |
| CA2981277A1 (en) * | 2015-03-30 | 2016-10-06 | Ajinomoto Co., Inc. | Human serum albumin-containing culture medium for growth of neural stem cells |
| WO2016183593A2 (en) * | 2015-05-14 | 2016-11-17 | The Regents Of The University Of California | Prenatal therapy |
| CN105002134B (zh) * | 2015-07-24 | 2018-08-17 | 北京大学 | 制备多潜能干细胞的组合物、方法、试剂盒及其用途 |
| CA2996582A1 (en) * | 2015-08-31 | 2017-03-09 | I Peace, Inc. | Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells |
| JP2018536438A (ja) | 2015-12-07 | 2018-12-13 | バイオタイム,インコーポレーテッド | 多様な多能性幹細胞から誘導された褐色脂肪細胞の再誘導のための方法 |
| WO2017124022A2 (en) * | 2016-01-13 | 2017-07-20 | Felix Pets, Llc | Methods for producing hypo-allergenic cats using gene editing technology |
| KR101855967B1 (ko) | 2016-03-11 | 2018-05-10 | 가톨릭관동대학교산학협력단 | 물리적 자극에 의한 환경유입을 이용한 세포 리프로그래밍 방법 |
| EP3436035B1 (en) * | 2016-03-29 | 2024-09-04 | SmsBiotech, Inc. | Compositions and methods for using small mobile stem cells |
| KR102711870B1 (ko) * | 2016-07-19 | 2024-10-02 | 아셀라타 리미티드 | 현탁 내 만능줄기세포를 배양하기 위한 배양 배지 |
| JP7588806B2 (ja) * | 2017-04-20 | 2024-11-25 | 国立大学法人東北大学 | 振盪浮遊培養を用いた間葉系幹細胞の未分化性維持方法 |
| WO2019181999A1 (ja) * | 2018-03-20 | 2019-09-26 | 富士フイルム株式会社 | 多能性幹細胞を三次元培養する方法 |
| KR102175792B1 (ko) * | 2018-11-20 | 2020-11-06 | 사회복지법인 삼성생명공익재단 | 줄기세포의 증식능을 탐지하기 위한 마커 및 이를 이용한 줄기세포의 고효율 증식방법 |
| WO2020206187A1 (en) * | 2019-04-02 | 2020-10-08 | Centagen, Inc | Engineered system of stem cell rejuvenation to treat aging and disease |
| WO2020206409A1 (en) | 2019-04-04 | 2020-10-08 | Synaptec Network, Inc. | Systems and methods for using low intensity ultrasonic transducer on the brain |
| JP2022534295A (ja) * | 2019-05-30 | 2022-07-28 | イミュノルクス インターナショナル コーポレーション | ワクチン接種及び疾患治療法のための自己人工多能性幹細胞とのポックスウイルスの使用 |
| JP7742836B2 (ja) * | 2019-12-09 | 2025-09-22 | デウン ファーマシューティカル カンパニー リミテッド | ヒト多能性幹細胞から間葉系幹細胞の製造方法およびこれによって製造された間葉系幹細胞 |
| JP7753883B2 (ja) * | 2020-01-14 | 2025-10-15 | 味の素株式会社 | 細胞培養用培地組成物 |
| KR20220128648A (ko) * | 2020-01-14 | 2022-09-21 | 아지노모토 가부시키가이샤 | 세포의 배양 방법 |
| CN115135755A (zh) * | 2020-02-12 | 2022-09-30 | 株式会社钟化 | 多能干细胞分化的抑制方法 |
| CN116096860A (zh) * | 2020-07-02 | 2023-05-09 | 阿塞尔塔有限公司 | 来自延迟胚胎的哺乳动物牲畜多能干细胞系 |
| US20220033771A1 (en) * | 2020-08-03 | 2022-02-03 | So Young Life Sciences Corporation | Combination Treatment Of Induced Pluripotent Stem Cells Using Interleukins |
| CA3221435A1 (en) * | 2021-06-09 | 2022-12-15 | Michal Amit | Serum free media for suspension culture of mammalian livestock pluripotent stem cells |
| CN114350599B (zh) * | 2022-01-14 | 2024-01-19 | 内蒙古大学 | 一种增强牛胚胎干细胞的多能性和代谢活力的培养体系和培养方法 |
| EP4532686A1 (en) * | 2022-05-24 | 2025-04-09 | Bluerock Therapeutics LP | Methods of making oligodendrocyte progenitor cells |
| WO2024102954A1 (en) | 2022-11-10 | 2024-05-16 | Massachusetts Institute Of Technology | Activation induced clipping system (aics) |
| JPWO2024247915A1 (enExample) * | 2023-05-26 | 2024-12-05 | ||
| WO2025006477A2 (en) * | 2023-06-27 | 2025-01-02 | The Board Of Regents Of The University Of Texas System | System and methods for generation of human fetal progenitors, tissues, and organs from cultured stem cells |
| WO2025106665A1 (en) * | 2023-11-15 | 2025-05-22 | Immorta Bio, Inc. | Generation of personalized mesenchymal stem cells |
| WO2025235862A1 (en) | 2024-05-10 | 2025-11-13 | Inndura Therapeutics Inc. | A modified immune cell receptor comprising a target-binding domain and the extracellular domain of cd16a |
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-
2011
- 2011-09-07 US US13/821,244 patent/US10214722B2/en active Active
- 2011-09-07 CA CA3150342A patent/CA3150342A1/en active Pending
- 2011-09-07 SG SG2013016365A patent/SG188411A1/en unknown
- 2011-09-07 JP JP2013527735A patent/JP6283223B2/ja active Active
- 2011-09-07 EP EP19181517.4A patent/EP3572501B1/en active Active
- 2011-09-07 CA CA2810488A patent/CA2810488C/en active Active
- 2011-09-07 DK DK11770876.8T patent/DK2614141T3/da active
- 2011-09-07 WO PCT/IL2011/000722 patent/WO2012032521A2/en not_active Ceased
- 2011-09-07 SG SG10202109393R patent/SG10202109393RA/en unknown
- 2011-09-07 SG SG10201800628QA patent/SG10201800628QA/en unknown
- 2011-09-07 SG SG10201509953VA patent/SG10201509953VA/en unknown
- 2011-09-07 ES ES11770876T patent/ES2746482T3/es active Active
- 2011-09-07 EP EP11770876.8A patent/EP2614141B1/en active Active
-
2013
- 2013-03-06 IL IL225070A patent/IL225070B/en active IP Right Grant
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2017
- 2017-01-11 US US15/403,303 patent/US10597635B2/en active Active
- 2017-06-08 JP JP2017113227A patent/JP6574458B2/ja active Active
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2018
- 2018-08-05 US US16/055,110 patent/US11193108B2/en active Active
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2019
- 2019-02-28 IL IL265107A patent/IL265107B/en active IP Right Grant
- 2019-08-16 JP JP2019149488A patent/JP2020000241A/ja active Pending
-
2020
- 2020-03-05 US US16/809,771 patent/US11959098B2/en active Active
- 2020-04-27 IL IL274282A patent/IL274282B/en unknown
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2021
- 2021-11-10 US US17/522,988 patent/US20220056406A1/en active Pending
- 2021-11-17 IL IL288185A patent/IL288185B2/en unknown
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2022
- 2022-01-20 JP JP2022007129A patent/JP7357086B2/ja active Active
-
2023
- 2023-09-25 JP JP2023161645A patent/JP7663644B2/ja active Active
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