IL225070B - New methods and mediums for growing pluripotent stem cells - Google Patents

New methods and mediums for growing pluripotent stem cells

Info

Publication number
IL225070B
IL225070B IL225070A IL22507013A IL225070B IL 225070 B IL225070 B IL 225070B IL 225070 A IL225070 A IL 225070A IL 22507013 A IL22507013 A IL 22507013A IL 225070 B IL225070 B IL 225070B
Authority
IL
Israel
Prior art keywords
stem cells
culture media
pluripotent stem
novel methods
culturing pluripotent
Prior art date
Application number
IL225070A
Other languages
English (en)
Hebrew (he)
Inventor
Amit Michal
Itskovitz-Eldor Joseph
Original Assignee
Technion Res & Dev Foundation
Amit Michal
Joseph Itskovitz Eldor
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technion Res & Dev Foundation, Amit Michal, Joseph Itskovitz Eldor filed Critical Technion Res & Dev Foundation
Publication of IL225070B publication Critical patent/IL225070B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2306—Interleukin-6 (IL-6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2311—Interleukin-11 (IL-11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Rheumatology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
IL225070A 2010-09-07 2013-03-06 New methods and mediums for growing pluripotent stem cells IL225070B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38038810P 2010-09-07 2010-09-07
PCT/IL2011/000722 WO2012032521A2 (en) 2010-09-07 2011-09-07 Novel methods and culture media for culturing pluripotent stem cells

Publications (1)

Publication Number Publication Date
IL225070B true IL225070B (en) 2019-03-31

Family

ID=44936324

Family Applications (4)

Application Number Title Priority Date Filing Date
IL225070A IL225070B (en) 2010-09-07 2013-03-06 New methods and mediums for growing pluripotent stem cells
IL265107A IL265107B (en) 2010-09-07 2019-02-28 New methods and mediums for growing pluripotent stem cells.
IL274282A IL274282B (en) 2010-09-07 2020-04-27 New methods and mediums for growing pluripotent stem cells
IL288185A IL288185B2 (en) 2010-09-07 2021-11-17 New methods and mediums for growing pluripotent stem cells

Family Applications After (3)

Application Number Title Priority Date Filing Date
IL265107A IL265107B (en) 2010-09-07 2019-02-28 New methods and mediums for growing pluripotent stem cells.
IL274282A IL274282B (en) 2010-09-07 2020-04-27 New methods and mediums for growing pluripotent stem cells
IL288185A IL288185B2 (en) 2010-09-07 2021-11-17 New methods and mediums for growing pluripotent stem cells

Country Status (9)

Country Link
US (5) US10214722B2 (enExample)
EP (2) EP3572501B1 (enExample)
JP (5) JP6283223B2 (enExample)
CA (2) CA3150342A1 (enExample)
DK (1) DK2614141T3 (enExample)
ES (1) ES2746482T3 (enExample)
IL (4) IL225070B (enExample)
SG (4) SG188411A1 (enExample)
WO (1) WO2012032521A2 (enExample)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3712258B1 (en) 2006-04-14 2025-01-01 Astellas Institute for Regenerative Medicine Hemangio-colony forming cells
EP2499236B1 (en) 2009-11-12 2020-01-01 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
US10214722B2 (en) 2010-09-07 2019-02-26 Technion Research & Development Foundation Limited Methods for expanding and maintaining human pluripotent stem cells (PSCs) in an undifferentiated state in a single cell suspension culture
JP5745919B2 (ja) * 2011-04-28 2015-07-08 浜松ホトニクス株式会社 細胞解析方法、細胞解析装置、および細胞解析プログラム
US9175263B2 (en) * 2012-08-22 2015-11-03 Biotime, Inc. Methods and compositions for targeting progenitor cell lines
EP3003290B1 (en) 2013-06-05 2021-03-10 AgeX Therapeutics, Inc. Compositions for use in the treatment of wounds in mammalian species
US11078462B2 (en) 2014-02-18 2021-08-03 ReCyte Therapeutics, Inc. Perivascular stromal cells from primate pluripotent stem cells
WO2015181253A1 (en) * 2014-05-27 2015-12-03 Fundación Pública Andaluza Progreso Y Salud Neural progenitor cell population
US10240127B2 (en) 2014-07-03 2019-03-26 ReCyte Therapeutics, Inc. Exosomes from clonal progenitor cells
US9944894B2 (en) 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
CA2981277A1 (en) * 2015-03-30 2016-10-06 Ajinomoto Co., Inc. Human serum albumin-containing culture medium for growth of neural stem cells
WO2016183593A2 (en) * 2015-05-14 2016-11-17 The Regents Of The University Of California Prenatal therapy
CN105002134B (zh) * 2015-07-24 2018-08-17 北京大学 制备多潜能干细胞的组合物、方法、试剂盒及其用途
CA2996582A1 (en) * 2015-08-31 2017-03-09 I Peace, Inc. Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells
JP2018536438A (ja) 2015-12-07 2018-12-13 バイオタイム，インコーポレーテッド 多様な多能性幹細胞から誘導された褐色脂肪細胞の再誘導のための方法
WO2017124022A2 (en) * 2016-01-13 2017-07-20 Felix Pets, Llc Methods for producing hypo-allergenic cats using gene editing technology
KR101855967B1 (ko) 2016-03-11 2018-05-10 가톨릭관동대학교산학협력단 물리적 자극에 의한 환경유입을 이용한 세포 리프로그래밍 방법
EP3436035B1 (en) * 2016-03-29 2024-09-04 SmsBiotech, Inc. Compositions and methods for using small mobile stem cells
KR102711870B1 (ko) * 2016-07-19 2024-10-02 아셀라타 리미티드 현탁 내 만능줄기세포를 배양하기 위한 배양 배지
JP7588806B2 (ja) * 2017-04-20 2024-11-25 国立大学法人東北大学 振盪浮遊培養を用いた間葉系幹細胞の未分化性維持方法
WO2019181999A1 (ja) * 2018-03-20 2019-09-26 富士フイルム株式会社 多能性幹細胞を三次元培養する方法
KR102175792B1 (ko) * 2018-11-20 2020-11-06 사회복지법인 삼성생명공익재단 줄기세포의 증식능을 탐지하기 위한 마커 및 이를 이용한 줄기세포의 고효율 증식방법
WO2020206187A1 (en) * 2019-04-02 2020-10-08 Centagen, Inc Engineered system of stem cell rejuvenation to treat aging and disease
WO2020206409A1 (en) 2019-04-04 2020-10-08 Synaptec Network, Inc. Systems and methods for using low intensity ultrasonic transducer on the brain
JP2022534295A (ja) * 2019-05-30 2022-07-28 イミュノルクス インターナショナル コーポレーション ワクチン接種及び疾患治療法のための自己人工多能性幹細胞とのポックスウイルスの使用
JP7742836B2 (ja) * 2019-12-09 2025-09-22 デウン ファーマシューティカル カンパニー リミテッド ヒト多能性幹細胞から間葉系幹細胞の製造方法およびこれによって製造された間葉系幹細胞
JP7753883B2 (ja) * 2020-01-14 2025-10-15 味の素株式会社 細胞培養用培地組成物
KR20220128648A (ko) * 2020-01-14 2022-09-21 아지노모토 가부시키가이샤 세포의 배양 방법
CN115135755A (zh) * 2020-02-12 2022-09-30 株式会社钟化 多能干细胞分化的抑制方法
CN116096860A (zh) * 2020-07-02 2023-05-09 阿塞尔塔有限公司 来自延迟胚胎的哺乳动物牲畜多能干细胞系
US20220033771A1 (en) * 2020-08-03 2022-02-03 So Young Life Sciences Corporation Combination Treatment Of Induced Pluripotent Stem Cells Using Interleukins
CA3221435A1 (en) * 2021-06-09 2022-12-15 Michal Amit Serum free media for suspension culture of mammalian livestock pluripotent stem cells
CN114350599B (zh) * 2022-01-14 2024-01-19 内蒙古大学 一种增强牛胚胎干细胞的多能性和代谢活力的培养体系和培养方法
EP4532686A1 (en) * 2022-05-24 2025-04-09 Bluerock Therapeutics LP Methods of making oligodendrocyte progenitor cells
WO2024102954A1 (en) 2022-11-10 2024-05-16 Massachusetts Institute Of Technology Activation induced clipping system (aics)
JPWO2024247915A1 (enExample) * 2023-05-26 2024-12-05
WO2025006477A2 (en) * 2023-06-27 2025-01-02 The Board Of Regents Of The University Of Texas System System and methods for generation of human fetal progenitors, tissues, and organs from cultured stem cells
WO2025106665A1 (en) * 2023-11-15 2025-05-22 Immorta Bio, Inc. Generation of personalized mesenchymal stem cells
WO2025235862A1 (en) 2024-05-10 2025-11-13 Inndura Therapeutics Inc. A modified immune cell receptor comprising a target-binding domain and the extracellular domain of cd16a

Family Cites Families (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
IL68218A (en) 1983-03-23 1985-12-31 Univ Ramot Compositions for cartilage repair comprising embryonal chondrocytes
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US4866042A (en) 1987-11-18 1989-09-12 Neuwelt Edward A Method for the delivery of genetic material across the blood brain barrier
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
US5489304A (en) 1994-04-19 1996-02-06 Brigham & Women's Hospital Method of skin regeneration using a collagen-glycosaminoglycan matrix and cultured epithelial autograft
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
AU3392697A (en) * 1996-06-14 1998-01-07 Regents Of The University Of California, The (in vitro) derivation and culture of primate pluripotent stem cells and therapeutic uses thereof
EP0986635A4 (en) 1997-01-10 2001-11-07 Life Technologies Inc SERUM SUBSTITUTE FOR EMBRYONIC STEM CELLS
US6090622A (en) 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
IL122818A0 (en) 1997-07-10 1998-08-16 Yeda Res & Dev Chimeric interleukin-6 soluble receptor/ligand protein analogs thereof and uses thereof
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US7455983B2 (en) * 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
IL159580A0 (en) 2001-07-12 2004-06-01 Geron Corp Cells of the cardiomyocyte lineage produced from human pluripotent stem cells
JPWO2003014336A1 (ja) 2001-08-07 2004-11-25 麒麟麦酒株式会社 造血幹細胞の製造法
WO2003046141A2 (en) * 2001-11-26 2003-06-05 Advanced Cell Technology, Inc. Methods for making and using reprogrammed human somatic cell nuclei and autologous and isogenic human stem cells
FR2836924B1 (fr) 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
US20070053890A1 (en) * 2003-03-28 2007-03-08 Sunnybrook And Women's College Health Sciences Centre Canine embryonic stem cells
JP4787156B2 (ja) 2003-07-22 2011-10-05 ビバリス 付着または非付着の鳥類細胞系を用いた、ポックスウィルスの作製法
WO2005065354A2 (en) 2003-12-31 2005-07-21 The Burnham Institute Defined media for pluripotent stem cell culture
JP4688793B2 (ja) * 2004-03-23 2011-05-25 敏宏 赤池 多能性幹細胞の増殖方法
RU2252252C1 (ru) * 2004-04-09 2005-05-20 Тепляшин Александр Сергеевич Способ выделения мезенхимальных стволовых клеток
DE102004043256B4 (de) 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
EP1799810A2 (en) 2004-10-12 2007-06-27 Technion Research & Development Foundation Ltd. Isolated primate embryonic cells and methods of generating and using same
JP2008538276A (ja) 2005-01-28 2008-10-23 ノヴァセラ・リミテッド 胚幹細胞培養のための方法
WO2006084314A1 (en) 2005-02-09 2006-08-17 Australian Stem Cell Centre Limited Stem cell populations and classification system
GB0505970D0 (en) 2005-03-23 2005-04-27 Univ Edinburgh Culture medium containing kinase inhibitor, and uses thereof
EP1962719A4 (en) 2005-08-29 2011-05-04 Technion Res And Dev Of Foundation Ltd MEDIA FOR BREEDING STEM CELLS
GB0615327D0 (en) * 2006-03-30 2006-09-13 Univ Edinburgh Culture medium containing kinase inhibitors and uses thereof
DK3441459T3 (da) 2006-08-02 2021-06-07 Technion Res & Dev Foundation Fremgangsmåder til ekspansion af embryonale stamceller i en suspensionskultur
WO2008129563A2 (en) * 2007-04-23 2008-10-30 Stempeutics Research Private Limited, Human mesenchymal stem cells and preparation thereof
KR20100059789A (ko) 2007-06-29 2010-06-04 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 배아줄기세포 배양을 위한 자동화 방법 및 장치
WO2010108126A2 (en) 2009-03-19 2010-09-23 Fate Therapeutics, Inc. Reprogramming compositions and methods of using the same
US8524498B2 (en) * 2009-05-29 2013-09-03 The General Hospital Corporation Methods and compositions for homologous recombination in human cells
EP2499236B1 (en) 2009-11-12 2020-01-01 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
US10214722B2 (en) 2010-09-07 2019-02-26 Technion Research & Development Foundation Limited Methods for expanding and maintaining human pluripotent stem cells (PSCs) in an undifferentiated state in a single cell suspension culture

Also Published As

Publication number Publication date
US10214722B2 (en) 2019-02-26
US20130236961A1 (en) 2013-09-12
CA2810488C (en) 2025-05-06
JP2013536696A (ja) 2013-09-26
US20180334650A1 (en) 2018-11-22
SG188411A1 (en) 2013-04-30
IL288185B1 (en) 2023-03-01
WO2012032521A2 (en) 2012-03-15
US20220056406A1 (en) 2022-02-24
JP7357086B2 (ja) 2023-10-05
JP2023179560A (ja) 2023-12-19
WO2012032521A3 (en) 2012-07-19
CA2810488A1 (en) 2012-03-15
US11959098B2 (en) 2024-04-16
IL274282B (en) 2021-12-01
IL288185B2 (en) 2023-07-01
US11193108B2 (en) 2021-12-07
ES2746482T3 (es) 2020-03-06
JP6283223B2 (ja) 2018-02-21
JP7663644B2 (ja) 2025-04-16
US10597635B2 (en) 2020-03-24
SG10201800628QA (en) 2018-02-27
EP2614141A2 (en) 2013-07-17
EP2614141B1 (en) 2019-07-31
IL288185A (en) 2022-01-01
DK2614141T3 (da) 2019-09-09
SG10201509953VA (en) 2016-01-28
US20200224157A1 (en) 2020-07-16
US20170183628A1 (en) 2017-06-29
JP6574458B2 (ja) 2019-09-11
CA3150342A1 (en) 2012-03-15
WO2012032521A8 (en) 2012-05-31
JP2022058686A (ja) 2022-04-12
SG10202109393RA (en) 2021-10-28
IL274282A (en) 2020-06-30
EP3572501B1 (en) 2025-07-16
IL265107B (en) 2020-05-31
JP2020000241A (ja) 2020-01-09
JP2017195892A (ja) 2017-11-02
EP3572501A1 (en) 2019-11-27

Similar Documents

Publication Publication Date Title
IL288185A (en) New methods and mediums for growing pluripotent stem cells
IL290485A (en) Substrate for the culture of stem cells
IL252607A0 (en) Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
IL214529A0 (en) Bioreactor for the cultivation of mammalian cells and methods of use thereof
EP2398896A4 (en) CELL CULTURE MEDIUM CONTAINING COMBINATIONS OF PROTEINS
IL216902A0 (en) Methods for culturing stem and progentitor cells
IL214381A0 (en) Culture medium for epithelial stem cells and organoids comprising said stem cells
SG10201502242VA (en) Culture method for causing differentiation of pluripotent mammalian cells
EP2594636A4 (en) CULTURE MEDIUM COMPOSITION FOR CULTURING MESENCHYMAL STEM CELLS FROM AMNIOTIC MEMBRANE, AND METHOD FOR CULTURING THE SAME USING THE SAME
GB201117469D0 (en) Culture media for pluripotent stem cells
GB2460552B (en) Stem cell culture media and methods
EP2768940A4 (en) SYSTEM AND METHOD FOR PREPARING MEDIA FOR CELL CULTURE BOXES
IL243634A0 (en) Method and medium for in vitro growth of stem cells
GB2482612B (en) Cell culture support for culturing mesenchymal stem cells
ZA201504388B (en) Method for culturing pluripotent stem cells
HK40121311A (zh) 用於干细胞的培养基
AU2010901922A0 (en) Media and methods for cell culture
GB201105503D0 (en) Microcarrier for the culture if stem cells, method of culturing stem cells and method of using said microcarrier
IL219736A0 (en) Colture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
AU2009901852A0 (en) Media and methods for cell culture
GB201007855D0 (en) Bioreactor for the culture of cells
GB0821363D0 (en) Stem cell culture media and methods
GB0810304D0 (en) Stem cell culture media and methods
GB201007863D0 (en) Bioreactor for cell culture
GB201007861D0 (en) Dynamic bioreactor for cell culture

Legal Events

Date Code Title Description
FF Patent granted
KB Patent renewed
KB Patent renewed