EP2594636A4 - Culture medium composition for culturing amnion-derived mesenchymal stem cell, and method for culturing amnion-derived mesenchymal stem cell by using same - Google Patents

Culture medium composition for culturing amnion-derived mesenchymal stem cell, and method for culturing amnion-derived mesenchymal stem cell by using same

Info

Publication number
EP2594636A4
EP2594636A4 EP11807097.8A EP11807097A EP2594636A4 EP 2594636 A4 EP2594636 A4 EP 2594636A4 EP 11807097 A EP11807097 A EP 11807097A EP 2594636 A4 EP2594636 A4 EP 2594636A4
Authority
EP
European Patent Office
Prior art keywords
stem cell
mesenchymal stem
derived mesenchymal
amnion
culturing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP11807097.8A
Other languages
German (de)
French (fr)
Other versions
EP2594636A2 (en
EP2594636B1 (en
Inventor
Jeong Chan Ra
Sung Keun Kang
Ju Yeon Seo
Hyoeun Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RNL Bio Co Ltd
Original Assignee
RNL Bio Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RNL Bio Co Ltd filed Critical RNL Bio Co Ltd
Publication of EP2594636A2 publication Critical patent/EP2594636A2/en
Publication of EP2594636A4 publication Critical patent/EP2594636A4/en
Application granted granted Critical
Publication of EP2594636B1 publication Critical patent/EP2594636B1/en
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
EP11807097.8A 2010-07-16 2011-07-18 Culture medium composition for culturing amnion-derived mesenchymal stem cell, and method for culturing amnion-derived mesenchymal stem cell by using same Not-in-force EP2594636B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020100068959A KR101211913B1 (en) 2010-07-16 2010-07-16 Medium for Culturing Mesenchymal Stem Cells Derived from Amnion and Method for Culturing Mesenchymal Stem Cells Derived from Amnion Using thereof
PCT/KR2011/005258 WO2012008813A2 (en) 2010-07-16 2011-07-18 Culture medium composition for culturing amnion-derived mesenchymal stem cell, and method for culturing amnion-derived mesenchymal stem cell by using same

Publications (3)

Publication Number Publication Date
EP2594636A2 EP2594636A2 (en) 2013-05-22
EP2594636A4 true EP2594636A4 (en) 2014-01-22
EP2594636B1 EP2594636B1 (en) 2014-11-26

Family

ID=45469969

Family Applications (1)

Application Number Title Priority Date Filing Date
EP11807097.8A Not-in-force EP2594636B1 (en) 2010-07-16 2011-07-18 Culture medium composition for culturing amnion-derived mesenchymal stem cell, and method for culturing amnion-derived mesenchymal stem cell by using same

Country Status (10)

Country Link
US (1) US8871513B2 (en)
EP (1) EP2594636B1 (en)
JP (1) JP5750159B2 (en)
KR (1) KR101211913B1 (en)
CN (1) CN103154241B (en)
AU (1) AU2011277156B2 (en)
BR (1) BR112013001089A2 (en)
ES (1) ES2527253T3 (en)
SG (1) SG187114A1 (en)
WO (1) WO2012008813A2 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101382566B1 (en) * 2012-02-01 2014-04-10 동아대학교 산학협력단 Methods for Culturing Mesenchymal Stem Cell Derived from Placenta Amniotic Membrane and Compositions Comprising Mesenchymal Stem Cell for Repairing Peripheral arterial disease
JP5926447B2 (en) 2012-04-18 2016-05-25 アール バイオ カンパニー リミテッドＲ Ｂｉｏ Ｃｏ．， Ｌｔｄ． Method for producing stem cells having a size suitable for intravascular administration
KR101523040B1 (en) * 2012-06-26 2015-05-26 라정찬 Method for Preparing High Concentration of Stem Cells
KR20140038236A (en) * 2012-09-20 2014-03-28 메디포스트(주) Method for preparing mesenchymal stem cell aggregates
CN103333856B (en) * 2013-07-25 2015-08-26 张潇潇 Human umbilical cord mesenchymal stem cell culture medium
CN104371970B (en) * 2013-08-15 2017-08-04 协和华东干细胞基因工程有限公司 A kind of culture medium for being used to cultivate human amnion membrane
CN104450608B (en) * 2013-09-12 2017-10-24 佛教慈济医疗财团法人 Promote the adjuvant of Human Meta's stem cell proliferation, expand Human Meta's stem cell methods, medical composition, growth factor and application thereof
CN103966159B (en) * 2014-02-13 2015-08-05 天津和泽干细胞科技有限公司 Human plactnta Subaerial blue green algae and stem cell bank construction process thereof
WO2015170347A2 (en) 2014-05-09 2015-11-12 Reelabs Private Limited Foetal polymix of mesenchymal stem cells under hypoxic conditions for the treatment of clinical disorders
CA2958533C (en) 2014-08-21 2023-03-14 Ajinomoto Co., Inc. Culture medium for mesenchymal stem cells
EP3252146B1 (en) * 2014-10-29 2021-09-15 R Bio Co., Ltd Medium composition for culturing stem cells
CN106032527B (en) * 2015-03-17 2019-08-13 广州市搏克肿瘤研究所 It is a kind of tolerance low-density without feeder layer human pluripotent stem cells culture medium
CN105176912B (en) * 2015-08-28 2019-02-22 广州赛莱拉干细胞科技股份有限公司 A kind of culture medium and its application, preparation method
KR101779932B1 (en) * 2015-09-15 2017-09-21 주식회사 스템랩 Method for producing of composition for promoting hair growth from human amniotic fluid-derived mesenchymal stem cells with nanog
CN105420184A (en) * 2015-12-11 2016-03-23 郭镭 Method for culturing umbilical cord mesenchymal stem cells in separated mode from umbilical cord outer layer amnion tissue
CN105754936B (en) * 2016-02-25 2018-06-08 付清玲 Method of people's inductive pluripotent stem cells induction for mescenchymal stem cell
US10894947B1 (en) * 2016-04-29 2021-01-19 Hope Biosciences, Llc Method for generating protein rich conditioned medium
US11111480B2 (en) * 2016-04-29 2021-09-07 Hope Biosctences, Llc Culture media for multipotent stem cells
US10959425B2 (en) * 2016-04-29 2021-03-30 Hope Biosciences, Llc Method of banking stem cells
CN110484500A (en) * 2019-09-23 2019-11-22 上海亚载生物科技有限公司 A method of separation and amplification human urine source mescenchymal stem cell
JP7348892B2 (en) * 2019-12-30 2023-09-21 佛教慈濟醫療財團法人 Compositions and methods for preventing or treating eye diseases
GB2596216A (en) * 2021-05-09 2021-12-22 Zhejiang Legou Tech Co Ltd An elliptical trainer with protective function

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005085422A1 (en) * 2004-02-27 2005-09-15 Michigan State University Adult stem cells and uses thereof
WO2009061024A1 (en) * 2007-11-09 2009-05-14 Rnl Bio Co., Ltd Method for isolating and culturing adult stem cells derived from human amniotic epithelium

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080152629A1 (en) * 2000-12-06 2008-06-26 James Edinger Placental stem cell populations
ES2527293T3 (en) 2004-08-16 2015-01-22 Cellresearch Corporation Pte Ltd Isolation of stem / progenitor cells of amniotic membrane of the umbilical cord
KR100679642B1 (en) * 2005-11-16 2007-02-06 주식회사 알앤엘바이오 Multipotent stem cell derived from human adipose tissue and cellular therapeutic agents comprising the same
ES2711278T3 (en) * 2005-12-29 2019-04-30 Celularity Inc Populations of placental stem cells
KR100818214B1 (en) 2006-09-29 2008-04-01 재단법인서울대학교산학협력재단 Multipotent stem cell derived from amnion or decidua isolated from human term placenta and method for preparing the same
US20080159999A1 (en) * 2006-10-23 2008-07-03 Konstantinos Stefanidis Compositions and methods for identifying, isolating and enriching germline-like stem cells from amniotic fluid
KR100795708B1 (en) 2006-12-26 2008-01-17 주식회사 알앤엘바이오 Method for isolating and culturing adult stem cells derived from human amniotic epithelium

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005085422A1 (en) * 2004-02-27 2005-09-15 Michigan State University Adult stem cells and uses thereof
WO2009061024A1 (en) * 2007-11-09 2009-05-14 Rnl Bio Co., Ltd Method for isolating and culturing adult stem cells derived from human amniotic epithelium

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2012008813A2 *

Also Published As

Publication number Publication date
BR112013001089A2 (en) 2019-09-24
WO2012008813A3 (en) 2012-05-03
EP2594636A2 (en) 2013-05-22
CN103154241B (en) 2015-05-20
AU2011277156A1 (en) 2013-02-07
SG187114A1 (en) 2013-02-28
KR101211913B1 (en) 2012-12-13
JP5750159B2 (en) 2015-07-15
WO2012008813A2 (en) 2012-01-19
US8871513B2 (en) 2014-10-28
EP2594636B1 (en) 2014-11-26
ES2527253T3 (en) 2015-01-21
US20130164849A1 (en) 2013-06-27
CN103154241A (en) 2013-06-12
JP2013530721A (en) 2013-08-01
AU2011277156B2 (en) 2014-06-05
KR20120008223A (en) 2012-01-30

Similar Documents

Publication Publication Date Title
EP2594636A4 (en) Culture medium composition for culturing amnion-derived mesenchymal stem cell, and method for culturing amnion-derived mesenchymal stem cell by using same
IL273159A (en) Culture medium composition, and method for culturing cell or tissue using said composition
IL288185B1 (en) Novel methods and culture media for culturing pluripotent stem cells
ZA201606403B (en) Pluripotent stem cell culture on micro-carriers
EP2752484A4 (en) Method for preparing a basic culture medium for mesenchymal stem cells, basic culture medium for mesenchymal stem cells, and cell therapeutic agent cultured and differentiated using same
EP2545928A4 (en) Cell preparation containing mesenchymal stem cells, and method for producing same
HK1171790A1 (en) Cell culture medium for adamts protein expression adamts
EP2644694A4 (en) Method and culture medium for improving pluripotent stem cell differentiation inducing efficiency
HK1222198A1 (en) Culture medium composition for improving regenerative capacity of stem cells, and stem cell culturing method using same
EP2843041A4 (en) Stem cell culture medium and method for culturing stem cells using same
GB2497059B (en) Culture medium, method for culturing listeria,and method for dtecting listeria
GB201117469D0 (en) Culture media for pluripotent stem cells
EP2351833A4 (en) Peptide-containing culture medium for culturing animal cell
BR112014000042A2 (en) composition, cell culture medium, use of a composition, and method for culturing cells
EP2638158A4 (en) Compositions, cells, kits and methods for autologous stem cell therapy
GB2482612B (en) Cell culture support for culturing mesenchymal stem cells
EP2511364A4 (en) Method for culturing cells or microorganisms
EP2695934A4 (en) Animal cell culture kit, method for culturing animal cells, method for selective culture of animal cells and cell differentiation method
EP2476748A4 (en) Culture method for hematopoietic stem cells
GB201007861D0 (en) Dynamic bioreactor for cell culture
GB201105442D0 (en) 3D cell culture method, composition and use for lead candidate selection
GB201007863D0 (en) Bioreactor for cell culture

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20130115

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20131220

RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 5/02 20060101ALI20131216BHEP

Ipc: C12N 5/0775 20100101AFI20131216BHEP

17Q First examination report despatched

Effective date: 20140526

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTG Intention to grant announced

Effective date: 20140806

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 698227

Country of ref document: AT

Kind code of ref document: T

Effective date: 20141215

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602011011799

Country of ref document: DE

Effective date: 20150108

REG Reference to a national code

Ref country code: ES

Ref legal event code: FG2A

Ref document number: 2527253

Country of ref document: ES

Kind code of ref document: T3

Effective date: 20150121

REG Reference to a national code

Ref country code: NL

Ref legal event code: VDEP

Effective date: 20141126

REG Reference to a national code

Ref country code: AT

Ref legal event code: MK05

Ref document number: 698227

Country of ref document: AT

Kind code of ref document: T

Effective date: 20141126

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150326

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150326

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150226

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: RS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

Ref country code: AT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150227

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 602011011799

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed

Effective date: 20150827

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150718

REG Reference to a national code

Ref country code: IE

Ref legal event code: MM4A

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150731

Ref country code: LI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150731

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 6

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150718

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: ES

Payment date: 20160613

Year of fee payment: 6

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20110718

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 7

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 8

REG Reference to a national code

Ref country code: ES

Ref legal event code: FD2A

Effective date: 20181026

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141126

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20180720

Year of fee payment: 8

Ref country code: DE

Payment date: 20180719

Year of fee payment: 8

Ref country code: FR

Payment date: 20180726

Year of fee payment: 8

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: TR

Payment date: 20180718

Year of fee payment: 8

Ref country code: GB

Payment date: 20180718

Year of fee payment: 8

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: ES

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170719

REG Reference to a national code

Ref country code: DE

Ref legal event code: R119

Ref document number: 602011011799

Country of ref document: DE

GBPC Gb: european patent ceased through non-payment of renewal fee

Effective date: 20190718

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GB

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20190718

Ref country code: DE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20200201

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FR

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20190731

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20190718

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20190718