JP2013530689A5 - - Google Patents

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Publication number
JP2013530689A5
JP2013530689A5 JP2013513806A JP2013513806A JP2013530689A5 JP 2013530689 A5 JP2013530689 A5 JP 2013530689A5 JP 2013513806 A JP2013513806 A JP 2013513806A JP 2013513806 A JP2013513806 A JP 2013513806A JP 2013530689 A5 JP2013530689 A5 JP 2013530689A5
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JP
Japan
Prior art keywords
nucleic acid
acid molecule
sharing
sequence
exonuclease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2013513806A
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English (en)
Japanese (ja)
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JP2013530689A (ja
JP5934196B2 (ja
Filing date
Publication date
Priority claimed from GBGB1009732.7A external-priority patent/GB201009732D0/en
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Publication of JP2013530689A publication Critical patent/JP2013530689A/ja
Publication of JP2013530689A5 publication Critical patent/JP2013530689A5/ja
Application granted granted Critical
Publication of JP5934196B2 publication Critical patent/JP5934196B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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JP2013513806A 2010-06-10 2011-06-10 直接クローニング Active JP5934196B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1009732.7 2010-06-10
GBGB1009732.7A GB201009732D0 (en) 2010-06-10 2010-06-10 Direct cloning
PCT/IB2011/052549 WO2011154927A2 (en) 2010-06-10 2011-06-10 Direct cloning

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2016093169A Division JP2016135158A (ja) 2010-06-10 2016-05-06 直接クローニング

Publications (3)

Publication Number Publication Date
JP2013530689A JP2013530689A (ja) 2013-08-01
JP2013530689A5 true JP2013530689A5 (enExample) 2014-07-10
JP5934196B2 JP5934196B2 (ja) 2016-06-15

Family

ID=42471456

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2013513806A Active JP5934196B2 (ja) 2010-06-10 2011-06-10 直接クローニング
JP2016093169A Pending JP2016135158A (ja) 2010-06-10 2016-05-06 直接クローニング

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2016093169A Pending JP2016135158A (ja) 2010-06-10 2016-05-06 直接クローニング

Country Status (8)

Country Link
US (2) US10443051B2 (enExample)
EP (2) EP2580338B1 (enExample)
JP (2) JP5934196B2 (enExample)
CN (2) CN103068995B (enExample)
CA (1) CA2802167C (enExample)
GB (1) GB201009732D0 (enExample)
SG (1) SG186219A1 (enExample)
WO (1) WO2011154927A2 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
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GB201009732D0 (en) 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
EP2612918A1 (en) * 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta recombination
CN102558309B (zh) * 2012-02-10 2014-09-17 浙江大学 一对转录激活子样效应因子核酸酶及其编码基因与应用
CN102627691B (zh) * 2012-04-05 2014-09-17 浙江大学 一对转录激活子样效应因子核酸酶及其编码基因与应用
CN104364380B (zh) 2012-04-25 2018-10-09 瑞泽恩制药公司 核酸酶介导的使用大靶向载体的靶向
CN102702335B (zh) * 2012-05-23 2014-07-09 上海斯丹赛生物技术有限公司 重组转录激活子样效应因子、转录激活子样效应因子核酸酶及其编码基因及应用
CN105209601A (zh) 2012-10-16 2015-12-30 基因桥有限责任公司 电感受态细胞及其制备
CA2908697C (en) 2013-04-16 2023-12-12 Regeneron Pharmaceuticals, Inc. Targeted modification of rat genome
SG10201700961TA (en) 2013-12-11 2017-04-27 Regeneron Pharma Methods and compositions for the targeted modification of a genome
EP3083958B1 (en) * 2013-12-19 2019-04-17 Amyris, Inc. Methods for genomic integration
HUE049776T2 (hu) 2014-06-06 2020-10-28 Regeneron Pharma Módszerek és készítmények egy célzott lókusz módosítására
MX384887B (es) 2014-06-23 2025-03-14 Regeneron Pharma Ensamblaje de adn mediado por nucleasa.
EP3161128B1 (en) 2014-06-26 2018-09-26 Regeneron Pharmaceuticals, Inc. Methods and compositions for targeted genetic modifications and methods of use
EP3221457B1 (en) 2014-11-21 2019-03-20 Regeneron Pharmaceuticals, Inc. Methods and compositions for targeted genetic modification using paired guide rnas
CN105713885B (zh) * 2014-12-04 2019-06-11 清华大学 特异识别并修复β地中海贫血症beta-globin基因的嵌合核酸酶
JP6840077B2 (ja) 2014-12-19 2021-03-10 リジェネロン・ファーマシューティカルズ・インコーポレイテッドRegeneron Pharmaceuticals, Inc. 単一ステップの複数標的化を通じた標的化された遺伝子修飾のための方法及び組成物
CN105734061B (zh) * 2016-03-25 2019-07-23 中国农业科学院生物技术研究所 从毕赤酵母中分离的鼠李糖诱导型启动子及其应用
CN109715813A (zh) * 2016-04-06 2019-05-03 基因桥有限公司 腺病毒载体
CN106995813B (zh) * 2017-03-23 2020-06-16 山东大学 基因组大片段直接克隆和dna多分子组装新技术
CN109385417A (zh) * 2017-08-03 2019-02-26 华东理工大学 体内dna无缝组装方法
CA3136114A1 (en) * 2019-04-05 2020-10-08 Danisco Us Inc. Methods for integrating a donor dna sequence into the genome of bacillus using linear recombinant dna constructs and compositions thereof
CN110184287B (zh) * 2019-05-24 2024-01-30 华南农业大学 一种制备重组病毒的方法及其应用
MX2021014861A (es) 2019-06-25 2022-06-22 Inari Agriculture Tech Inc Edicion genomica de reparacion dependiente de homologia mejorada.
CN115717152A (zh) * 2022-11-29 2023-02-28 江南大学 一种控制枯草芽孢杆菌基因组同源重组概率的方法

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US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5776744A (en) 1995-06-07 1998-07-07 Yale University Methods and compositions for effecting homologous recombination
AU752105B2 (en) * 1997-12-05 2002-09-05 Europaisches Laboratorium Fur Molekularbiologie (Embl) Novel dna cloning method
AUPP684998A0 (en) 1998-10-30 1998-11-26 Murdoch Institute for Research into Birth Defects Limited, The A method of recombination and agents useful for same
US6355412B1 (en) * 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
US6569681B1 (en) * 2000-03-14 2003-05-27 Transkaryotic Therapies, Inc. Methods of improving homologous recombination
US20040101520A1 (en) 2001-02-09 2004-05-27 Muijrers Joep P P Recombination method
EP1409702A1 (en) 2001-07-20 2004-04-21 Youming Zhang Improved rect or recet cloning and subcloning method
WO2004067753A2 (en) * 2003-01-28 2004-08-12 Cellectis Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof.
WO2007013979A2 (en) 2005-07-20 2007-02-01 Invitrogen Corporation Methods for increasing efficiency of homologous recombination
KR100681815B1 (ko) * 2005-12-02 2007-02-12 한국생명공학연구원 리컴비네이즈 매개 선택표지 유전자 발현유도에 의한 목표유전자 간편 클로닝 및 목표 단백질 신속 발현 방법.
US10407672B2 (en) 2006-03-27 2019-09-10 Seattle Children's Hospital Compositions and methods comprising the use of cell surface displayed homing endonucleases
GB0803109D0 (en) 2008-02-20 2008-03-26 Gene Bridges Gmbh Method of nucleic acid recombination
JP2011217736A (ja) * 2010-03-26 2011-11-04 Aichi Medical Univ 組換えクラミジア及びその製造方法
GB201009732D0 (en) * 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning

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