JP2013503639A5 - - Google Patents
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- JP2013503639A5 JP2013503639A5 JP2012528040A JP2012528040A JP2013503639A5 JP 2013503639 A5 JP2013503639 A5 JP 2013503639A5 JP 2012528040 A JP2012528040 A JP 2012528040A JP 2012528040 A JP2012528040 A JP 2012528040A JP 2013503639 A5 JP2013503639 A5 JP 2013503639A5
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- Prior art keywords
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- direct chemical
- cells
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- Prior art date
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- 239000000203 mixture Substances 0.000 claims 30
- 238000000034 method Methods 0.000 claims 15
- 239000000126 substance Substances 0.000 claims 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 10
- 238000003556 assay Methods 0.000 claims 10
- 230000009089 cytolysis Effects 0.000 claims 9
- 239000000872 buffer Substances 0.000 claims 8
- 238000004090 dissolution Methods 0.000 claims 7
- 239000000523 sample Substances 0.000 claims 6
- 239000012472 biological sample Substances 0.000 claims 5
- 239000002736 nonionic surfactant Substances 0.000 claims 5
- 150000003839 salts Chemical class 0.000 claims 5
- 239000011780 sodium chloride Substances 0.000 claims 5
- 229910052751 metal Inorganic materials 0.000 claims 4
- 239000002184 metal Substances 0.000 claims 4
- 239000004094 surface-active agent Substances 0.000 claims 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims 2
- 239000002202 Polyethylene glycol Substances 0.000 claims 2
- 239000012491 analyte Substances 0.000 claims 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 229920001223 polyethylene glycol Polymers 0.000 claims 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims 1
- 238000009007 Diagnostic Kit Methods 0.000 claims 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims 1
- 235000011130 ammonium sulphate Nutrition 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 claims 1
- 230000002380 cytological effect Effects 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 235000019253 formic acid Nutrition 0.000 claims 1
- 150000004676 glycans Chemical class 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- ZPIRTVJRHUMMOI-UHFFFAOYSA-N octoxybenzene Chemical compound CCCCCCCCOC1=CC=CC=C1 ZPIRTVJRHUMMOI-UHFFFAOYSA-N 0.000 claims 1
- 229920001308 poly(aminoacid) Polymers 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 229920001282 polysaccharide Polymers 0.000 claims 1
- 239000005017 polysaccharide Substances 0.000 claims 1
- 235000011164 potassium chloride Nutrition 0.000 claims 1
- 239000001103 potassium chloride Substances 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 239000001632 sodium acetate Substances 0.000 claims 1
- 235000017281 sodium acetate Nutrition 0.000 claims 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23955309P | 2009-09-03 | 2009-09-03 | |
| US61/239,553 | 2009-09-03 | ||
| PCT/US2010/047653 WO2011028887A2 (en) | 2009-09-03 | 2010-09-02 | Methods and compositions for direct chemical lysis |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013503639A JP2013503639A (ja) | 2013-02-04 |
| JP2013503639A5 true JP2013503639A5 (enExample) | 2013-10-24 |
| JP5916610B2 JP5916610B2 (ja) | 2016-05-11 |
Family
ID=43648071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012528040A Active JP5916610B2 (ja) | 2009-09-03 | 2010-09-02 | 直接的な化学的溶解のための方法および組成物 |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US10190152B2 (enExample) |
| EP (1) | EP2473596B1 (enExample) |
| JP (1) | JP5916610B2 (enExample) |
| CN (1) | CN102575220B (enExample) |
| AU (1) | AU2010289430B2 (enExample) |
| BR (1) | BR112012004699B1 (enExample) |
| CA (1) | CA2772621C (enExample) |
| DK (1) | DK2473596T3 (enExample) |
| ES (1) | ES2660762T3 (enExample) |
| WO (1) | WO2011028887A2 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2473596T3 (en) * | 2009-09-03 | 2018-01-22 | Becton Dickinson Co | METHODS AND COMPOSITIONS FOR DIRECT CHEMICAL LYSIS |
| CN103421891B (zh) * | 2013-03-26 | 2016-03-02 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | 一种用于PCR检测的血浆miRNA制备方法 |
| CA2961613A1 (en) * | 2014-09-17 | 2016-03-24 | Hologic, Inc. | Method of partial lysis and assay |
| CN104862305A (zh) * | 2015-06-17 | 2015-08-26 | 河南省农业科学院畜牧兽医研究所 | 一种用于动物组织基因组dna和rna快速提取试剂盒及提取方法和应用 |
| EP3222719B8 (en) * | 2016-03-24 | 2021-03-17 | Allflex Europe SAS | Use of an aqueous composition for dissolving biomolecules from a tissue sample |
| CN107723240A (zh) * | 2016-08-12 | 2018-02-23 | 微基生物科技(上海)有限公司 | 一种皮肤、口腔、生殖道样品中的微生物保存试剂及其制备方法和应用 |
| US10792416B2 (en) | 2017-05-30 | 2020-10-06 | Haemonetics Corporation | System and method for collecting plasma |
| CN118853827A (zh) | 2018-10-01 | 2024-10-29 | 贝克顿迪金森公司 | 确定5’转录物序列 |
| CN110272898B (zh) * | 2019-07-11 | 2023-06-09 | 上海市浦东新区疾病预防控制中心 | 一种通用型微生物病原体裂解液及其应用 |
| CN111057704B (zh) * | 2019-12-31 | 2022-07-05 | 广州市金圻睿生物科技有限责任公司 | 一种石蜡切片样本dna提取的脱蜡裂解结合液、试剂盒及方法 |
| CN115379793A (zh) * | 2020-04-21 | 2022-11-22 | 美国西门子医学诊断股份有限公司 | 用于临床样本采集和分子诊断应用的运送培养基及其生产和使用方法 |
| CN113957122B (zh) * | 2020-07-21 | 2024-01-26 | 厦门赛尔吉亚医学检验所有限公司 | 一种在宫颈细胞样本中提取dna的方法 |
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| DE2532183C3 (de) | 1975-07-18 | 1982-03-04 | Behringwerke Ag, 3550 Marburg | Polyionische isotonische Salzlösung zur Konservierung von Erythrozyten oder zur Perfusion und zur Konservierung von zur Transplantion bestimmter Organen |
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| US5248506A (en) | 1986-03-19 | 1993-09-28 | American National Red Cross | Synthetic, plasma-free, transfusible storage medium for red blood cells and platelets |
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| US5231015A (en) * | 1989-10-18 | 1993-07-27 | Eastman Kodak Company | Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme |
| US4997932A (en) | 1989-11-13 | 1991-03-05 | Boehringer Mannheim Corporation | Method and kit for purifying nucleic acids |
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| CA2218875C (en) | 1991-07-23 | 2000-11-07 | The Research Foundation Of State University Of New York | Improvements in the in situ pcr |
| AU664050B2 (en) | 1991-12-18 | 1995-11-02 | Becton Dickinson & Company | Process for lysing mycobacteria |
| DE69329353T2 (de) * | 1992-03-05 | 2001-01-04 | Becton Dickinson And Co., Franklin Lakes | Verfahren zur Herstellung und Analyse von Leukozyten in Vollblut unter Verwendung der Durchflusszytometrie |
| WO1994026867A1 (en) * | 1993-05-06 | 1994-11-24 | Abbott Laboratories | Direct lysis buffer and the detection of hiv-1 plasma viremia |
| CA2129773C (en) | 1993-09-02 | 2007-05-01 | Michael Klaus | Aromatic carboxylic acid derivatives |
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| WO1995014768A2 (en) | 1993-11-29 | 1995-06-01 | Gen-Probe Incorporated | Method for extracting nucleic acids from a wide range of organisms |
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-
2010
- 2010-09-02 DK DK10814484.1T patent/DK2473596T3/en active
- 2010-09-02 WO PCT/US2010/047653 patent/WO2011028887A2/en not_active Ceased
- 2010-09-02 BR BR112012004699-3A patent/BR112012004699B1/pt active IP Right Grant
- 2010-09-02 CA CA2772621A patent/CA2772621C/en active Active
- 2010-09-02 US US12/874,602 patent/US10190152B2/en active Active
- 2010-09-02 ES ES10814484.1T patent/ES2660762T3/es active Active
- 2010-09-02 JP JP2012528040A patent/JP5916610B2/ja active Active
- 2010-09-02 CN CN201080046134.7A patent/CN102575220B/zh active Active
- 2010-09-02 EP EP10814484.1A patent/EP2473596B1/en active Active
- 2010-09-02 AU AU2010289430A patent/AU2010289430B2/en active Active
-
2016
- 2016-03-11 US US15/067,428 patent/US10323267B2/en active Active
-
2019
- 2019-04-30 US US16/399,305 patent/US11434519B2/en active Active
-
2022
- 2022-08-08 US US17/883,403 patent/US20220372554A1/en active Pending
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