CA2772621C - Methods and compositions for direct chemical lysis - Google Patents

Methods and compositions for direct chemical lysis Download PDF

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Publication number
CA2772621C
CA2772621C CA2772621A CA2772621A CA2772621C CA 2772621 C CA2772621 C CA 2772621C CA 2772621 A CA2772621 A CA 2772621A CA 2772621 A CA2772621 A CA 2772621A CA 2772621 C CA2772621 C CA 2772621C
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Canada
Prior art keywords
composition
sample
direct chemical
chemical lysis
cells
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CA2772621A
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English (en)
French (fr)
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CA2772621A1 (en
Inventor
Feng Yang
Sha-Sha Wang
Laurence Michael Vaughan
Michael Porter
Elaine Rose
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Becton Dickinson and Co
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Becton Dickinson and Co
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Publication of CA2772621A1 publication Critical patent/CA2772621A1/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/708Specific hybridization probes for papilloma
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983Viruses
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01DNA viruses
    • G01N2333/025Papovaviridae, e.g. papillomavirus, polyomavirus, SV40, BK virus, JC virus

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2772621A 2009-09-03 2010-09-02 Methods and compositions for direct chemical lysis Active CA2772621C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23955309P 2009-09-03 2009-09-03
US61/239,553 2009-09-03
PCT/US2010/047653 WO2011028887A2 (en) 2009-09-03 2010-09-02 Methods and compositions for direct chemical lysis

Publications (2)

Publication Number Publication Date
CA2772621A1 CA2772621A1 (en) 2011-03-10
CA2772621C true CA2772621C (en) 2019-11-12

Family

ID=43648071

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2772621A Active CA2772621C (en) 2009-09-03 2010-09-02 Methods and compositions for direct chemical lysis

Country Status (10)

Country Link
US (4) US10190152B2 (enExample)
EP (1) EP2473596B1 (enExample)
JP (1) JP5916610B2 (enExample)
CN (1) CN102575220B (enExample)
AU (1) AU2010289430B2 (enExample)
BR (1) BR112012004699B1 (enExample)
CA (1) CA2772621C (enExample)
DK (1) DK2473596T3 (enExample)
ES (1) ES2660762T3 (enExample)
WO (1) WO2011028887A2 (enExample)

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CN107723240A (zh) * 2016-08-12 2018-02-23 微基生物科技(上海)有限公司 一种皮肤、口腔、生殖道样品中的微生物保存试剂及其制备方法和应用
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CN118853827A (zh) 2018-10-01 2024-10-29 贝克顿迪金森公司 确定5’转录物序列
CN110272898B (zh) * 2019-07-11 2023-06-09 上海市浦东新区疾病预防控制中心 一种通用型微生物病原体裂解液及其应用
CN111057704B (zh) * 2019-12-31 2022-07-05 广州市金圻睿生物科技有限责任公司 一种石蜡切片样本dna提取的脱蜡裂解结合液、试剂盒及方法
CN115379793A (zh) * 2020-04-21 2022-11-22 美国西门子医学诊断股份有限公司 用于临床样本采集和分子诊断应用的运送培养基及其生产和使用方法
CN113957122B (zh) * 2020-07-21 2024-01-26 厦门赛尔吉亚医学检验所有限公司 一种在宫颈细胞样本中提取dna的方法

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Also Published As

Publication number Publication date
US10190152B2 (en) 2019-01-29
JP5916610B2 (ja) 2016-05-11
BR112012004699A2 (pt) 2015-09-01
CA2772621A1 (en) 2011-03-10
US11434519B2 (en) 2022-09-06
US20110059455A1 (en) 2011-03-10
EP2473596A4 (en) 2013-01-16
WO2011028887A2 (en) 2011-03-10
BR112012004699B1 (pt) 2019-10-15
AU2010289430B2 (en) 2015-06-25
AU2010289430A1 (en) 2012-03-22
DK2473596T3 (en) 2018-01-22
US10323267B2 (en) 2019-06-18
JP2013503639A (ja) 2013-02-04
US20220372554A1 (en) 2022-11-24
ES2660762T3 (es) 2018-03-26
US20190256893A1 (en) 2019-08-22
EP2473596A2 (en) 2012-07-11
WO2011028887A3 (en) 2011-07-28
EP2473596B1 (en) 2017-12-13
US20160194687A1 (en) 2016-07-07
CN102575220A (zh) 2012-07-11
CN102575220B (zh) 2015-09-16

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