JP2012531428A - 哺乳類以外の系で発現されるタンパク質の捕獲精製プロセス - Google Patents

哺乳類以外の系で発現されるタンパク質の捕獲精製プロセス Download PDF

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JP2012531428A
JP2012531428A JP2012517737A JP2012517737A JP2012531428A JP 2012531428 A JP2012531428 A JP 2012531428A JP 2012517737 A JP2012517737 A JP 2012517737A JP 2012517737 A JP2012517737 A JP 2012517737A JP 2012531428 A JP2012531428 A JP 2012531428A
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protein
separation matrix
proteins
refolding
solution
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JP2012531428A5 (enExample
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シユルツ，ジヨゼフ・エドワード
ハート，ロジヤー
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アムジエン・インコーポレーテツド
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
JP2012517737A 2009-06-25 2010-06-24 哺乳類以外の系で発現されるタンパク質の捕獲精製プロセス Pending JP2012531428A (ja)

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US22047709P 2009-06-25 2009-06-25
US61/220,477 2009-06-25
PCT/US2010/039854 WO2010151688A2 (en) 2009-06-25 2010-06-24 Capture purification processes for proteins expressed in a non-mammalian system

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JP2012531428A true JP2012531428A (ja) 2012-12-10
JP2012531428A5 JP2012531428A5 (enExample) 2013-08-08

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US (5) US8940878B2 (enExample)
EP (2) EP3660032B1 (enExample)
JP (1) JP2012531428A (enExample)
AU (1) AU2010266093B2 (enExample)
CA (1) CA2765154A1 (enExample)
ES (1) ES2862580T5 (enExample)
MX (1) MX2011013417A (enExample)
WO (1) WO2010151688A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190037247A (ko) * 2016-07-22 2019-04-05 암젠 인크 Fc-함유 단백질의 정제 방법
JP2022501357A (ja) * 2018-09-21 2022-01-06 テネオバイオ， インコーポレイテッド ヘテロ二量体多重特異性抗体を精製するための方法

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JP5808323B2 (ja) * 2009-06-22 2015-11-10 アムジエン・インコーポレーテツド 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング
EP3660032B1 (en) 2009-06-25 2025-10-22 Amgen Inc. Capture purification processes for proteins expressed in a non-mammalian system
PE20130557A1 (es) 2010-03-04 2013-05-19 Pfenex Inc Metodo para producir proteinas de interferon recombinantes solubles sin desnaturalizacion
GB201506870D0 (en) 2015-04-22 2015-06-03 Ucb Biopharma Sprl Method
US20170101435A1 (en) * 2015-10-13 2017-04-13 Therapeutic Proteins International, LLC Harvesting and perfusion apparatus
US11697670B2 (en) 2017-08-22 2023-07-11 Biogen Ma Inc. Methods for purifying antibodies having reduced high molecular weight aggregates
US11051532B2 (en) 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
US12011016B2 (en) 2020-09-14 2024-06-18 Impossible Foods Inc. Protein methods and compositions
AU2021399935A1 (en) 2020-12-18 2023-06-29 Richter Gedeon Nyrt. Methods for the purification of refolded fc-peptide fusion protein

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190037247A (ko) * 2016-07-22 2019-04-05 암젠 인크 Fc-함유 단백질의 정제 방법
JP2019528255A (ja) * 2016-07-22 2019-10-10 アムジエン・インコーポレーテツド Ｆｃ含有タンパク質を精製する方法
JP7092744B2 (ja) 2016-07-22 2022-06-28 アムジエン・インコーポレーテツド Ｆｃ含有タンパク質を精製する方法
KR102435801B1 (ko) * 2016-07-22 2022-08-25 암젠 인크 Fc-함유 단백질의 정제 방법
KR20220120716A (ko) * 2016-07-22 2022-08-30 암젠 인크 Fc-함유 단백질의 정제 방법
KR102579850B1 (ko) * 2016-07-22 2023-09-18 암젠 인크 Fc-함유 단백질의 정제 방법
JP2022501357A (ja) * 2018-09-21 2022-01-06 テネオバイオ， インコーポレイテッド ヘテロ二量体多重特異性抗体を精製するための方法

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US20200361986A1 (en) 2020-11-19
EP3660032A1 (en) 2020-06-03
CA2765154A1 (en) 2010-12-29
US11407784B2 (en) 2022-08-09
AU2010266093A1 (en) 2012-01-19
US12312381B2 (en) 2025-05-27
WO2010151688A2 (en) 2010-12-29
MX2011013417A (es) 2012-03-29
AU2010266093B2 (en) 2013-06-27
EP2445924B1 (en) 2021-03-03
US10577392B2 (en) 2020-03-03
ES2862580T3 (es) 2021-10-07
US20100331526A1 (en) 2010-12-30
US20150361130A1 (en) 2015-12-17
EP2445924B2 (en) 2023-12-13
US9643997B2 (en) 2017-05-09
US8940878B2 (en) 2015-01-27
ES2862580T5 (es) 2024-06-13
EP3660032B1 (en) 2025-10-22
US20180037603A1 (en) 2018-02-08
EP2445924A2 (en) 2012-05-02
WO2010151688A3 (en) 2011-05-19
US20230016430A1 (en) 2023-01-19

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