CA2765154A1 - Capture purification processes for proteins expressed in a non-mammalian system - Google Patents
Capture purification processes for proteins expressed in a non-mammalian system Download PDFInfo
- Publication number
- CA2765154A1 CA2765154A1 CA2765154A CA2765154A CA2765154A1 CA 2765154 A1 CA2765154 A1 CA 2765154A1 CA 2765154 A CA2765154 A CA 2765154A CA 2765154 A CA2765154 A CA 2765154A CA 2765154 A1 CA2765154 A1 CA 2765154A1
- Authority
- CA
- Canada
- Prior art keywords
- protein
- separation matrix
- native
- solution
- expressed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- 239000000243 solution Substances 0.000 claims description 68
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- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 20
- 239000003398 denaturant Substances 0.000 claims description 20
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 20
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/32—Extraction; Separation; Purification by precipitation as complexes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (3)
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| US22047709P | 2009-06-25 | 2009-06-25 | |
| US61/220,477 | 2009-06-25 | ||
| PCT/US2010/039854 WO2010151688A2 (en) | 2009-06-25 | 2010-06-24 | Capture purification processes for proteins expressed in a non-mammalian system |
Publications (1)
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|---|---|
| CA2765154A1 true CA2765154A1 (en) | 2010-12-29 |
Family
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| CA2765154A Abandoned CA2765154A1 (en) | 2009-06-25 | 2010-06-24 | Capture purification processes for proteins expressed in a non-mammalian system |
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| WO (1) | WO2010151688A2 (enExample) |
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| EP3660032B1 (en) | 2009-06-25 | 2025-10-22 | Amgen Inc. | Capture purification processes for proteins expressed in a non-mammalian system |
| PE20130557A1 (es) | 2010-03-04 | 2013-05-19 | Pfenex Inc | Metodo para producir proteinas de interferon recombinantes solubles sin desnaturalizacion |
| GB201506870D0 (en) | 2015-04-22 | 2015-06-03 | Ucb Biopharma Sprl | Method |
| US20170101435A1 (en) * | 2015-10-13 | 2017-04-13 | Therapeutic Proteins International, LLC | Harvesting and perfusion apparatus |
| IL285146B (en) * | 2016-07-22 | 2022-09-01 | Amgen Inc | Methods for purifying proteins involving fc |
| US11697670B2 (en) | 2017-08-22 | 2023-07-11 | Biogen Ma Inc. | Methods for purifying antibodies having reduced high molecular weight aggregates |
| US11051532B2 (en) | 2017-09-22 | 2021-07-06 | Impossible Foods Inc. | Methods for purifying protein |
| WO2020061478A2 (en) * | 2018-09-21 | 2020-03-26 | Teneobio, Inc. | Methods for purifying heterodimeric, multispecific antibodies |
| US12011016B2 (en) | 2020-09-14 | 2024-06-18 | Impossible Foods Inc. | Protein methods and compositions |
| AU2021399935A1 (en) | 2020-12-18 | 2023-06-29 | Richter Gedeon Nyrt. | Methods for the purification of refolded fc-peptide fusion protein |
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- 2010-06-24 MX MX2011013417A patent/MX2011013417A/es active IP Right Grant
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2015
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2019
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2022
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Also Published As
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| US20200361986A1 (en) | 2020-11-19 |
| EP3660032A1 (en) | 2020-06-03 |
| US11407784B2 (en) | 2022-08-09 |
| AU2010266093A1 (en) | 2012-01-19 |
| US12312381B2 (en) | 2025-05-27 |
| WO2010151688A2 (en) | 2010-12-29 |
| JP2012531428A (ja) | 2012-12-10 |
| MX2011013417A (es) | 2012-03-29 |
| AU2010266093B2 (en) | 2013-06-27 |
| EP2445924B1 (en) | 2021-03-03 |
| US10577392B2 (en) | 2020-03-03 |
| ES2862580T3 (es) | 2021-10-07 |
| US20100331526A1 (en) | 2010-12-30 |
| US20150361130A1 (en) | 2015-12-17 |
| EP2445924B2 (en) | 2023-12-13 |
| US9643997B2 (en) | 2017-05-09 |
| US8940878B2 (en) | 2015-01-27 |
| ES2862580T5 (es) | 2024-06-13 |
| EP3660032B1 (en) | 2025-10-22 |
| US20180037603A1 (en) | 2018-02-08 |
| EP2445924A2 (en) | 2012-05-02 |
| WO2010151688A3 (en) | 2011-05-19 |
| US20230016430A1 (en) | 2023-01-19 |
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