JP2012518390A5 - - Google Patents
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- JP2012518390A5 JP2012518390A5 JP2011550396A JP2011550396A JP2012518390A5 JP 2012518390 A5 JP2012518390 A5 JP 2012518390A5 JP 2011550396 A JP2011550396 A JP 2011550396A JP 2011550396 A JP2011550396 A JP 2011550396A JP 2012518390 A5 JP2012518390 A5 JP 2012518390A5
- Authority
- JP
- Japan
- Prior art keywords
- cell
- antigen
- immunobinder
- cells
- expressing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims description 82
- 210000004027 cell Anatomy 0.000 claims description 76
- 239000000427 antigen Substances 0.000 claims description 54
- 108091007433 antigens Proteins 0.000 claims description 54
- 102000036639 antigens Human genes 0.000 claims description 54
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 32
- 241001465754 Metazoa Species 0.000 claims description 9
- 101710160107 Outer membrane protein A Proteins 0.000 claims description 8
- 150000007523 nucleic acids Chemical group 0.000 claims description 8
- 102000018697 Membrane Proteins Human genes 0.000 claims description 6
- 108010052285 Membrane Proteins Proteins 0.000 claims description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 4
- 102000011011 Sphingosine 1-phosphate receptors Human genes 0.000 claims description 4
- 108050001083 Sphingosine 1-phosphate receptors Proteins 0.000 claims description 4
- 238000000423 cell based assay Methods 0.000 claims description 4
- 239000013604 expression vector Substances 0.000 claims description 4
- 239000007850 fluorescent dye Substances 0.000 claims description 4
- 210000004962 mammalian cell Anatomy 0.000 claims description 4
- 210000005253 yeast cell Anatomy 0.000 claims description 4
- 108020004414 DNA Proteins 0.000 claims description 3
- 102000003688 G-Protein-Coupled Receptors Human genes 0.000 claims description 3
- 108090000045 G-Protein-Coupled Receptors Proteins 0.000 claims description 3
- 230000003053 immunization Effects 0.000 claims description 3
- 238000002372 labelling Methods 0.000 claims description 3
- 108020004707 nucleic acids Proteins 0.000 claims description 3
- 102000039446 nucleic acids Human genes 0.000 claims description 3
- AYMWCZFEDLLHMI-UHFFFAOYSA-N (2-Hydroxymethyl-cyclohexyl)-acetic acid lactone Chemical compound C1CCCC2COC(=O)CC21 AYMWCZFEDLLHMI-UHFFFAOYSA-N 0.000 claims description 2
- 108010006533 ATP-Binding Cassette Transporters Proteins 0.000 claims description 2
- 102000005416 ATP-Binding Cassette Transporters Human genes 0.000 claims description 2
- 102000003787 Anoctamin-1 Human genes 0.000 claims description 2
- 108090000160 Anoctamin-1 Proteins 0.000 claims description 2
- 108091008875 B cell receptors Proteins 0.000 claims description 2
- 102000012304 Bestrophin Human genes 0.000 claims description 2
- 108050002823 Bestrophin Proteins 0.000 claims description 2
- 102100031172 C-C chemokine receptor type 1 Human genes 0.000 claims description 2
- 101710149814 C-C chemokine receptor type 1 Proteins 0.000 claims description 2
- 102100031151 C-C chemokine receptor type 2 Human genes 0.000 claims description 2
- 101710149815 C-C chemokine receptor type 2 Proteins 0.000 claims description 2
- 102100024167 C-C chemokine receptor type 3 Human genes 0.000 claims description 2
- 101710149862 C-C chemokine receptor type 3 Proteins 0.000 claims description 2
- 102100037853 C-C chemokine receptor type 4 Human genes 0.000 claims description 2
- 101710149863 C-C chemokine receptor type 4 Proteins 0.000 claims description 2
- 102100035875 C-C chemokine receptor type 5 Human genes 0.000 claims description 2
- 101710149870 C-C chemokine receptor type 5 Proteins 0.000 claims description 2
- 102100036305 C-C chemokine receptor type 8 Human genes 0.000 claims description 2
- 102100025074 C-C chemokine receptor-like 2 Human genes 0.000 claims description 2
- 102100036166 C-X-C chemokine receptor type 1 Human genes 0.000 claims description 2
- 102100028989 C-X-C chemokine receptor type 2 Human genes 0.000 claims description 2
- 102100028990 C-X-C chemokine receptor type 3 Human genes 0.000 claims description 2
- 102100031650 C-X-C chemokine receptor type 4 Human genes 0.000 claims description 2
- 102100025618 C-X-C chemokine receptor type 6 Human genes 0.000 claims description 2
- 241000282836 Camelus dromedarius Species 0.000 claims description 2
- 108091006146 Channels Proteins 0.000 claims description 2
- 108010079245 Cystic Fibrosis Transmembrane Conductance Regulator Proteins 0.000 claims description 2
- 238000011238 DNA vaccination Methods 0.000 claims description 2
- 102000005915 GABA Receptors Human genes 0.000 claims description 2
- 108010005551 GABA Receptors Proteins 0.000 claims description 2
- 241000287828 Gallus gallus Species 0.000 claims description 2
- 102000011714 Glycine Receptors Human genes 0.000 claims description 2
- 108010076533 Glycine Receptors Proteins 0.000 claims description 2
- 101000716068 Homo sapiens C-C chemokine receptor type 6 Proteins 0.000 claims description 2
- 101000716063 Homo sapiens C-C chemokine receptor type 8 Proteins 0.000 claims description 2
- 101000947174 Homo sapiens C-X-C chemokine receptor type 1 Proteins 0.000 claims description 2
- 101000916050 Homo sapiens C-X-C chemokine receptor type 3 Proteins 0.000 claims description 2
- 101000922348 Homo sapiens C-X-C chemokine receptor type 4 Proteins 0.000 claims description 2
- 101000856683 Homo sapiens C-X-C chemokine receptor type 6 Proteins 0.000 claims description 2
- 101000631760 Homo sapiens Sodium channel protein type 1 subunit alpha Proteins 0.000 claims description 2
- 101000654356 Homo sapiens Sodium channel protein type 10 subunit alpha Proteins 0.000 claims description 2
- 101000640020 Homo sapiens Sodium channel protein type 11 subunit alpha Proteins 0.000 claims description 2
- 101000684826 Homo sapiens Sodium channel protein type 2 subunit alpha Proteins 0.000 claims description 2
- 101000684820 Homo sapiens Sodium channel protein type 3 subunit alpha Proteins 0.000 claims description 2
- 101000693993 Homo sapiens Sodium channel protein type 4 subunit alpha Proteins 0.000 claims description 2
- 101000694017 Homo sapiens Sodium channel protein type 5 subunit alpha Proteins 0.000 claims description 2
- 101000654381 Homo sapiens Sodium channel protein type 8 subunit alpha Proteins 0.000 claims description 2
- 108060003951 Immunoglobulin Proteins 0.000 claims description 2
- 108010018951 Interleukin-8B Receptors Proteins 0.000 claims description 2
- 108090000862 Ion Channels Proteins 0.000 claims description 2
- 102000004310 Ion Channels Human genes 0.000 claims description 2
- HOKKHZGPKSLGJE-GSVOUGTGSA-N N-Methyl-D-aspartic acid Chemical compound CN[C@@H](C(O)=O)CC(O)=O HOKKHZGPKSLGJE-GSVOUGTGSA-N 0.000 claims description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- 108010003723 Single-Domain Antibodies Proteins 0.000 claims description 2
- 102100028910 Sodium channel protein type 1 subunit alpha Human genes 0.000 claims description 2
- 102100031374 Sodium channel protein type 10 subunit alpha Human genes 0.000 claims description 2
- 102100033974 Sodium channel protein type 11 subunit alpha Human genes 0.000 claims description 2
- 102100023150 Sodium channel protein type 2 subunit alpha Human genes 0.000 claims description 2
- 102100023720 Sodium channel protein type 3 subunit alpha Human genes 0.000 claims description 2
- 102100027195 Sodium channel protein type 4 subunit alpha Human genes 0.000 claims description 2
- 102100027198 Sodium channel protein type 5 subunit alpha Human genes 0.000 claims description 2
- 102100031371 Sodium channel protein type 8 subunit alpha Human genes 0.000 claims description 2
- 101150052500 cic-1 gene Proteins 0.000 claims description 2
- 102000034287 fluorescent proteins Human genes 0.000 claims description 2
- 108091006047 fluorescent proteins Proteins 0.000 claims description 2
- 210000005260 human cell Anatomy 0.000 claims description 2
- 102000018358 immunoglobulin Human genes 0.000 claims description 2
- 102000008371 intracellularly ATP-gated chloride channel activity proteins Human genes 0.000 claims description 2
- 238000002955 isolation Methods 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 238000007860 single-cell PCR Methods 0.000 claims description 2
- 101001128969 Homo sapiens Neuron navigator 1 Proteins 0.000 claims 1
- 102100031225 Neuron navigator 1 Human genes 0.000 claims 1
- 101000654386 Homo sapiens Sodium channel protein type 9 subunit alpha Proteins 0.000 description 1
- 102100031367 Sodium channel protein type 9 subunit alpha Human genes 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15504109P | 2009-02-24 | 2009-02-24 | |
| US15510509P | 2009-02-24 | 2009-02-24 | |
| US61/155,105 | 2009-02-24 | ||
| US61/155,041 | 2009-02-24 | ||
| CH8322009 | 2009-06-02 | ||
| CH832/09 | 2009-06-02 | ||
| PCT/CH2009/000222 WO2009155726A2 (en) | 2008-06-25 | 2009-06-25 | Humanization of rabbit antibodies using a universal antibody framework |
| CHPCT/CH2009/000222 | 2009-06-25 | ||
| PCT/CH2010/000044 WO2010096941A1 (en) | 2009-02-24 | 2010-02-22 | Methods for identifying immunobinders of cell-surface antigens |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015101581A Division JP6161655B2 (ja) | 2009-02-24 | 2015-05-19 | 細胞表面抗原のイムノバインダーを同定するための方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012518390A JP2012518390A (ja) | 2012-08-16 |
| JP2012518390A5 true JP2012518390A5 (https=) | 2013-02-14 |
| JP5764071B2 JP5764071B2 (ja) | 2015-08-12 |
Family
ID=42631310
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011550396A Active JP5764071B2 (ja) | 2009-02-24 | 2010-02-22 | 細胞表面抗原のイムノバインダーを同定するための方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (7) | US8227199B2 (https=) |
| EP (2) | EP3181583A1 (https=) |
| JP (1) | JP5764071B2 (https=) |
| KR (1) | KR101595808B1 (https=) |
| CN (1) | CN102239181A (https=) |
| AU (1) | AU2010217120B2 (https=) |
| CA (1) | CA2744103C (https=) |
| IL (1) | IL212738A (https=) |
| MX (1) | MX2011007049A (https=) |
| WO (1) | WO2010096941A1 (https=) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX345039B (es) * | 2008-06-25 | 2017-01-16 | Esbatech Alcon Biomed Res Unit | Anticuerpos estables y solubles que inhiben el tnfa. |
| CN104004094B (zh) * | 2008-06-25 | 2017-09-22 | 艾斯巴技术-诺华有限责任公司 | 使用通用抗体构架进行的兔抗体的人源化 |
| EP3181583A1 (en) | 2009-02-24 | 2017-06-21 | ESBATech, an Alcon Biomedical Research Unit LLC | Methods for identifying immunobinders of antigens |
| TWI386645B (zh) * | 2010-07-19 | 2013-02-21 | Univ Kaohsiung Medical | 可定量任何聚乙二醇分子與其修飾物之抗聚乙二醇表現細胞 |
| US9329180B2 (en) * | 2010-07-19 | 2016-05-03 | Kaohsiung Medical University | Preparation of anti-PEG antibody expressing cell and application thereof |
| EA202092589A3 (ru) | 2010-11-08 | 2021-06-30 | Аблинкс Н.В. | Cxcr2-связывающие полипептиды |
| US8748587B2 (en) | 2011-06-02 | 2014-06-10 | Novartis Ag | Molecules and methods for modulating TMEM16A activities |
| CN103946236B (zh) * | 2011-11-23 | 2018-02-16 | 弗·哈夫曼-拉罗切有限公司 | 表达cd40l的哺乳动物细胞及其用途 |
| US9328174B2 (en) | 2012-05-09 | 2016-05-03 | Novartis Ag | Chemokine receptor binding polypeptides |
| US9045546B2 (en) | 2012-05-31 | 2015-06-02 | Novartis Ag | Molecules and methods for modulating TMEM16A activities |
| ES2920601T3 (es) | 2013-06-28 | 2022-08-05 | X Body Inc | Descubrimiento de antígenos objetivo, cribados fenotípicos y uso de los mismos para la identificación de epítopos objetivo específicos de células objetivo |
| CN105793705A (zh) * | 2013-09-30 | 2016-07-20 | X博迪公司 | 抗原受体的筛选测试 |
| CN111413298A (zh) * | 2020-05-27 | 2020-07-14 | 深圳市天安至远传感科技有限公司 | 一种光纤表面等离子体共振传感器、制备方法及检测系统 |
| US20230406919A1 (en) * | 2020-12-03 | 2023-12-21 | The Johns Hopkins University | Nanobodies with specific affinity for voltage gated sodium channels |
| WO2025257154A1 (en) | 2024-06-10 | 2025-12-18 | Charité - Universitätsmedizin Berlin Körperschaft des öffentlichen Rechts | Flow cytometry method for determining physically interacting cells |
Family Cites Families (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5225539A (en) | 1986-03-27 | 1993-07-06 | Medical Research Council | Recombinant altered antibodies and methods of making altered antibodies |
| US20030036092A1 (en) * | 1991-11-15 | 2003-02-20 | Board Of Regents, The University Of Texas System | Directed evolution of enzymes and antibodies |
| US5675063A (en) * | 1995-02-28 | 1997-10-07 | Loyola University Of Chicago | Immortalized rabbit hybridoma fusion partner |
| CA2219361C (en) | 1995-04-27 | 2012-02-28 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
| AU725609C (en) | 1995-08-18 | 2002-01-03 | Morphosys Ag | Protein/(poly)peptide libraries |
| CA2267620A1 (en) | 1996-07-16 | 1998-01-22 | Andreas Pluckthun | Immunoglobulin superfamily domains and fragments with increased solubility |
| WO1998049286A2 (en) * | 1997-05-01 | 1998-11-05 | Board Of Regents, The University Of Texas System | Directed evolution of enzymes and antibodies |
| US6060272A (en) * | 1997-05-07 | 2000-05-09 | Human Genome Sciences, Inc. | Human G-protein coupled receptors |
| FR2777007B1 (fr) * | 1998-04-01 | 2000-05-26 | Orstom | Mutants de glycoproteines d'enveloppes de retrovirus et leurs applications biologiques |
| US7157418B1 (en) * | 1998-07-22 | 2007-01-02 | Osprey Pharmaceuticals, Ltd. | Methods and compositions for treating secondary tissue damage and other inflammatory conditions and disorders |
| US6905668B1 (en) | 1998-09-25 | 2005-06-14 | Tokyo Gas Company Limited | Diagnostic agents for pancreatic exocrine function |
| DE19852800C1 (de) * | 1998-11-16 | 2000-04-13 | Univ Albert Ludwigs Freiburg | Verfahren zur Herstellung von Antikörpern gegen ein Polypeptid, von dem die kodierende Nukleinsäure bekannt ist |
| PL201533B1 (pl) * | 1999-01-13 | 2009-04-30 | Igeneon Krebs Immuntherapie | Zastosowanie przeciwciała skierowanego przeciwko antygenowi błony komórkowej Ep-CAM |
| DE60140474D1 (de) | 2000-09-08 | 2009-12-24 | Univ Zuerich | Sammlung von proteinen mit sich wiederholenden sequenzen (repeat proteins), die repetitive sequenzmodule enthalten |
| CN1487996B (zh) | 2000-11-30 | 2010-06-16 | 米德列斯公司 | 用于生产人类抗体的转基因转染色体啮齿动物 |
| SI2332989T1 (sl) | 2002-05-22 | 2015-06-30 | Esbatech, An Alcon Biomedical Research Unit Llc | Imunoglobulinski bralni okvirji, ki kaĺ˝ejo izboljĺ ano stabilnost v intraceliäśnem okolju, in postopki za njihovo identifikacijo |
| US20040067496A1 (en) * | 2002-10-07 | 2004-04-08 | Robert Pytela | System for production and screening of monoclonal antibodies |
| WO2004044584A1 (en) | 2002-11-13 | 2004-05-27 | Micromet Ag | Method for identifying antigen specific b cells |
| US7993864B2 (en) | 2002-12-03 | 2011-08-09 | Ucb Pharma S.A. | Assay for identifying antibody producing cells |
| JP4336812B2 (ja) * | 2003-03-07 | 2009-09-30 | 博夫 岩田 | 細胞に発現する表面抗原を迅速に同定するための分析方法 |
| DE10314412A1 (de) | 2003-03-28 | 2004-10-14 | Genovac Ag | Genetische Immunisierung mit multiplen Expressionskonstrukten zur Herstellung von monoklonalen Antikörpern |
| JP4317548B2 (ja) | 2003-05-13 | 2009-08-19 | 日清オイリオグループ株式会社 | 油性化粧料 |
| US20070087331A1 (en) * | 2003-05-15 | 2007-04-19 | Cytos Biotechnology Ag | Selection of b cells with specificity if interest: method of preparation and use |
| EP1651659A4 (en) * | 2003-08-07 | 2008-09-17 | Epitomics Inc | METHOD FOR HUMANIZATION OF MONOCLONAL RABBIT ANTIBODIES |
| JP2004107355A (ja) | 2003-10-20 | 2004-04-08 | Kokyu Alcohol Kogyo Co Ltd | 油性固形化粧料 |
| EP1686121B1 (en) | 2003-11-20 | 2010-02-10 | Mitsubishi Gas Chemical Company, Inc. | Liquid cyclohexane-tricarboxylic acid anhydride |
| WO2005103074A2 (en) * | 2004-03-18 | 2005-11-03 | Board Of Regents, The University Of Texas System | Combinatorial protein library screening by periplasmic expression |
| CA2600929A1 (en) * | 2005-03-04 | 2006-09-14 | Biogen Idec Ma Inc. | Methods of humanizing immunoglobulin variable regions through rational modification of complementarity determining residues |
| CA2655511C (en) * | 2005-07-01 | 2017-03-21 | John Schrader | Methods of isolating cells and generating monoclonal antibodies |
| US20070134249A1 (en) * | 2005-12-08 | 2007-06-14 | Genitope Corporation | Combination therapy and antibody panels |
| US8017119B2 (en) | 2005-12-28 | 2011-09-13 | Daiichi Sankyo Company, Ltd. | Antibody against periostin, and a pharmaceutical composition comprising it for preventing or treating a disease in which periostin is involved |
| EP1918302A3 (en) | 2006-05-18 | 2009-11-18 | AvantGen, Inc. | Methods for the identification and the isolation of epitope specific antibodies |
| ES2398076T3 (es) * | 2006-06-02 | 2013-03-13 | Regeneron Pharmaceuticals, Inc. | Anticuerpos de alta afinidad contra el receptor de IL-6 humano |
| JP2009542232A (ja) | 2006-07-10 | 2009-12-03 | エスバテック・アーゲー | 上皮および／または内皮層を通過するｓｃＦｖ抗体 |
| BRPI0720565A2 (pt) * | 2006-12-20 | 2013-09-17 | Mmr Information Systems Inc | anticorpos e mÉtodos para a sua preparaÇço e seu uso |
| JP2010222255A (ja) * | 2007-06-18 | 2010-10-07 | Mitsubishi Tanabe Pharma Corp | 抗体のスクリーニング方法およびそれにより得られる抗体 |
| JP5022818B2 (ja) | 2007-08-10 | 2012-09-12 | 株式会社 資生堂 | 毛髪化粧料 |
| LT3216803T (lt) | 2008-06-25 | 2020-06-10 | Novartis Ag | Stabilūs ir tirpūs antikūnai, slopinantys vegf |
| MX345039B (es) | 2008-06-25 | 2017-01-16 | Esbatech Alcon Biomed Res Unit | Anticuerpos estables y solubles que inhiben el tnfa. |
| CN104004094B (zh) | 2008-06-25 | 2017-09-22 | 艾斯巴技术-诺华有限责任公司 | 使用通用抗体构架进行的兔抗体的人源化 |
| EP3181583A1 (en) | 2009-02-24 | 2017-06-21 | ESBATech, an Alcon Biomedical Research Unit LLC | Methods for identifying immunobinders of antigens |
| JP5926896B2 (ja) | 2010-09-17 | 2016-05-25 | 株式会社 資生堂 | 唇用固形化粧料 |
-
2010
- 2010-02-22 EP EP16202099.4A patent/EP3181583A1/en not_active Withdrawn
- 2010-02-22 WO PCT/CH2010/000044 patent/WO2010096941A1/en not_active Ceased
- 2010-02-22 JP JP2011550396A patent/JP5764071B2/ja active Active
- 2010-02-22 CN CN2010800034510A patent/CN102239181A/zh active Pending
- 2010-02-22 CA CA2744103A patent/CA2744103C/en active Active
- 2010-02-22 EP EP10705086.6A patent/EP2321354B2/en active Active
- 2010-02-22 AU AU2010217120A patent/AU2010217120B2/en active Active
- 2010-02-22 MX MX2011007049A patent/MX2011007049A/es unknown
- 2010-02-22 KR KR1020117014643A patent/KR101595808B1/ko active Active
- 2010-02-23 US US12/710,579 patent/US8227199B2/en active Active
-
2011
- 2011-05-05 IL IL212738A patent/IL212738A/en active IP Right Grant
-
2012
- 2012-06-22 US US13/530,241 patent/US20120288878A1/en not_active Abandoned
- 2012-09-14 US US13/616,267 patent/US8465937B2/en active Active
-
2014
- 2014-05-08 US US14/273,231 patent/US9221905B2/en active Active
-
2015
- 2015-11-20 US US14/947,133 patent/US9908940B2/en active Active
-
2018
- 2018-01-17 US US15/873,022 patent/US20180142028A1/en not_active Abandoned
-
2019
- 2019-08-29 US US16/555,674 patent/US20190382498A1/en not_active Abandoned
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