JP2012508591A - 細胞を操作するための組成物および方法 - Google Patents
細胞を操作するための組成物および方法 Download PDFInfo
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| JP2018521643A (ja) * | 2015-06-10 | 2018-08-09 | アメリカン ジーン テクノロジーズ インターナショナル インコーポレイテッド | 非組み込みウイルス送達システムおよびその使用の方法 |
| JP2019520814A (ja) * | 2016-06-08 | 2019-07-25 | アメリカン ジーン テクノロジーズ インターナショナル インコーポレイテッド | 非組み込みウイルス送達系およびその関連方法 |
| US10767183B2 (en) | 2016-03-09 | 2020-09-08 | American Gene Technologies International Inc. | Combination vectors and methods for treating cancer |
| US10772905B2 (en) | 2016-01-15 | 2020-09-15 | American Gene Technologies International Inc. | Methods and compositions for the activation of gamma-delta T-cells |
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| US11519006B2 (en) | 2016-01-15 | 2022-12-06 | American Gene Technologies International Inc. | Methods and compositions for the activation of gamma-delta T-cells |
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| US11980663B2 (en) | 2015-07-08 | 2024-05-14 | American Gene Technologies International Inc. | HIV pre-immunization and immunotherapy |
| US12559769B2 (en) | 2017-06-16 | 2026-02-24 | American Gene Technologies International Inc. | Methods and compositions for the activation of tumor cytotoxicity via human gamma-delta t-cells |
| JP7855208B2 (ja) | 2016-06-08 | 2026-05-08 | アメリカン ジーン テクノロジーズ インターナショナル インコーポレイテッド | 非組み込みウイルス送達系およびその関連方法 |
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| US9487756B2 (en) * | 2009-03-20 | 2016-11-08 | Mesoblast, Inc. | Production of reprogrammed pluripotent cells |
| WO2011119942A1 (en) * | 2010-03-25 | 2011-09-29 | Vistagen Therapeutics, Inc. | Induction of ips cells using transient episomal vectors |
| AU2013212068B2 (en) | 2012-01-26 | 2018-02-15 | Life Technologies Corporation | Methods for increasing the infectivity of viruses |
| KR20140128966A (ko) * | 2012-01-26 | 2014-11-06 | 라이프 테크놀로지스 코포레이션 | 바이러스의 감염성을 증가시키는 방법 |
| SG11201500799YA (en) * | 2012-07-31 | 2015-03-30 | Basil M Hantash | Hla g-modified cells and methods |
| WO2016014794A1 (en) * | 2014-07-25 | 2016-01-28 | Sangamo Biosciences, Inc. | Methods and compositions for modulating nuclease-mediated genome engineering in hematopoietic stem cells |
| RU2716991C2 (ru) | 2014-11-05 | 2020-03-17 | Вояджер Терапьютикс, Инк. | Полинуклеотиды aadc для лечения болезни паркинсона |
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Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| US5175385A (en) | 1987-09-03 | 1992-12-29 | Ohio University/Edison Animal Biotechnolgy Center | Virus-resistant transgenic mice |
| US5175384A (en) | 1988-12-05 | 1992-12-29 | Genpharm International | Transgenic mice depleted in mature t-cells and methods for making transgenic mice |
| US5453357A (en) | 1992-10-08 | 1995-09-26 | Vanderbilt University | Pluripotential embryonic stem cells and methods of making same |
| US5871986A (en) | 1994-09-23 | 1999-02-16 | The General Hospital Corporation | Use of a baculovirus to express and exogenous gene in a mammalian cell |
| EP2322614A1 (en) | 1995-06-07 | 2011-05-18 | Life Technologies Corporation | Recombinational cloning using engineered recombination sites |
| US6143557A (en) | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
| FR2749857B1 (fr) * | 1996-06-12 | 1998-08-14 | Centre Nat Rech Scient | Generation de molecules replicatives in vivo |
| US5851808A (en) | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
| US5998204A (en) | 1997-03-14 | 1999-12-07 | The Regents Of The University Of California | Fluorescent protein sensors for detection of analytes |
| US7351578B2 (en) | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
| WO2000011155A1 (en) * | 1998-08-19 | 2000-03-02 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for genomic modification |
| US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| US8972161B1 (en) | 2005-11-17 | 2015-03-03 | Invent.Ly, Llc | Power management systems and devices |
| EP2103685A1 (en) * | 2008-03-20 | 2009-09-23 | Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | Vectors and methods for generating vector-free induced pluripotent stem (iPS) cells using site-specific recombination |
| EP3279314A1 (en) * | 2008-06-04 | 2018-02-07 | Cellular Dynamics International, Inc. | Methods for the production of ips cells using non-viral approach |
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2015
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| US10975374B2 (en) | 2016-03-09 | 2021-04-13 | American Gene Technologies International Inc. | Combination vectors and methods for treating cancer |
| JP7173548B2 (ja) | 2016-06-08 | 2022-11-16 | アメリカン ジーン テクノロジーズ インターナショナル インコーポレイテッド | 非組み込みウイルス送達系およびその関連方法 |
| US11976292B2 (en) | 2016-06-08 | 2024-05-07 | American Gene Technologies International Inc. | Non-integrating viral delivery system and methods related thereto |
| JP2019523767A (ja) * | 2016-06-08 | 2019-08-29 | アメリカン ジーン テクノロジーズ インターナショナル インコーポレイテッド | 非組み込みウイルス送達系およびその関連方法 |
| JP2019520814A (ja) * | 2016-06-08 | 2019-07-25 | アメリカン ジーン テクノロジーズ インターナショナル インコーポレイテッド | 非組み込みウイルス送達系およびその関連方法 |
| JP7855208B2 (ja) | 2016-06-08 | 2026-05-08 | アメリカン ジーン テクノロジーズ インターナショナル インコーポレイテッド | 非組み込みウイルス送達系およびその関連方法 |
| US11911458B2 (en) | 2016-07-08 | 2024-02-27 | American Gene Technologies International Inc. | HIV pre-immunization and immunotherapy |
| US11090379B2 (en) | 2016-07-08 | 2021-08-17 | American Gene Technologies International Inc. | HIV pre-immunization and immunotherapy |
| US12370253B2 (en) | 2016-07-08 | 2025-07-29 | American Gene Technologies International Inc. | Pre-immunization and immunotherapy |
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| US12559769B2 (en) | 2017-06-16 | 2026-02-24 | American Gene Technologies International Inc. | Methods and compositions for the activation of tumor cytotoxicity via human gamma-delta t-cells |
Also Published As
| Publication number | Publication date |
|---|---|
| US20160010113A1 (en) | 2016-01-14 |
| WO2011071476A3 (en) | 2011-12-08 |
| CN102369288A (zh) | 2012-03-07 |
| EP2362912A1 (en) | 2011-09-07 |
| WO2011071476A2 (en) | 2011-06-16 |
| US20130040304A1 (en) | 2013-02-14 |
| US20110263001A1 (en) | 2011-10-27 |
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