JP2012508562A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2012508562A5 JP2012508562A5 JP2011535656A JP2011535656A JP2012508562A5 JP 2012508562 A5 JP2012508562 A5 JP 2012508562A5 JP 2011535656 A JP2011535656 A JP 2011535656A JP 2011535656 A JP2011535656 A JP 2011535656A JP 2012508562 A5 JP2012508562 A5 JP 2012508562A5
- Authority
- JP
- Japan
- Prior art keywords
- insulin
- free
- medium
- fvii
- serum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 198
- 238000000034 method Methods 0.000 claims description 134
- 239000002609 medium Substances 0.000 claims description 108
- 102000004877 Insulin Human genes 0.000 claims description 99
- 229940125396 insulin Drugs 0.000 claims description 99
- 108090001061 Insulin Proteins 0.000 claims description 98
- 210000004027 cell Anatomy 0.000 claims description 89
- 102100023804 Coagulation factor VII Human genes 0.000 claims description 77
- 108010023321 Factor VII Proteins 0.000 claims description 77
- 229940012413 factor vii Drugs 0.000 claims description 77
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims description 36
- 229920001184 polypeptide Polymers 0.000 claims description 26
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 26
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 26
- 239000012679 serum free medium Substances 0.000 claims description 15
- 210000004962 mammalian cell Anatomy 0.000 claims description 13
- 238000004113 cell culture Methods 0.000 claims description 11
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 230000003247 decreasing effect Effects 0.000 claims description 7
- 238000010899 nucleation Methods 0.000 claims description 6
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 claims description 4
- 241001465754 Metazoa Species 0.000 claims description 4
- 238000012136 culture method Methods 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 239000012737 fresh medium Substances 0.000 claims description 4
- 230000010412 perfusion Effects 0.000 claims description 4
- KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 claims description 4
- 238000011218 seed culture Methods 0.000 claims description 4
- ABSPRNADVQNDOU-UHFFFAOYSA-N Menaquinone 1 Natural products C1=CC=C2C(=O)C(CC=C(C)C)=C(C)C(=O)C2=C1 ABSPRNADVQNDOU-UHFFFAOYSA-N 0.000 claims description 3
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 claims description 3
- MBWXNTAXLNYFJB-NKFFZRIASA-N phylloquinone Chemical compound C1=CC=C2C(=O)C(C/C=C(C)/CCC[C@H](C)CCC[C@H](C)CCCC(C)C)=C(C)C(=O)C2=C1 MBWXNTAXLNYFJB-NKFFZRIASA-N 0.000 claims description 3
- 235000019175 phylloquinone Nutrition 0.000 claims description 3
- 239000011772 phylloquinone Substances 0.000 claims description 3
- 229960001898 phytomenadione Drugs 0.000 claims description 3
- 238000004062 sedimentation Methods 0.000 claims description 3
- 210000002966 serum Anatomy 0.000 claims description 3
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 claims description 2
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims description 2
- 241000699802 Cricetulus griseus Species 0.000 claims description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 2
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 claims description 2
- 239000005700 Putrescine Substances 0.000 claims description 2
- 239000000654 additive Substances 0.000 claims description 2
- 239000006143 cell culture medium Substances 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims description 2
- 239000012228 culture supernatant Substances 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 claims description 2
- 229940099816 human factor vii Drugs 0.000 claims description 2
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims description 2
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims description 2
- 238000011031 large-scale manufacturing process Methods 0.000 claims description 2
- 230000007774 longterm Effects 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 210000001672 ovary Anatomy 0.000 claims description 2
- 229960003531 phenolsulfonphthalein Drugs 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims description 2
- 238000004114 suspension culture Methods 0.000 claims description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims 2
- 239000000969 carrier Substances 0.000 claims 2
- 238000010923 batch production Methods 0.000 claims 1
- 208000002352 blister Diseases 0.000 claims 1
- 230000007423 decrease Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 230000002062 proliferating effect Effects 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims 1
- 235000017557 sodium bicarbonate Nutrition 0.000 claims 1
- 238000006467 substitution reaction Methods 0.000 claims 1
- 229940088594 vitamin Drugs 0.000 claims 1
- 229930003231 vitamin Natural products 0.000 claims 1
- 235000013343 vitamin Nutrition 0.000 claims 1
- 239000011782 vitamin Substances 0.000 claims 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11379208P | 2008-11-12 | 2008-11-12 | |
| US61/113,792 | 2008-11-12 | ||
| PCT/US2009/063338 WO2010056584A1 (en) | 2008-11-12 | 2009-11-05 | Method of producing serum-free insulin-free factor vii |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012508562A JP2012508562A (ja) | 2012-04-12 |
| JP2012508562A5 true JP2012508562A5 (https=) | 2012-12-20 |
| JP5719301B2 JP5719301B2 (ja) | 2015-05-13 |
Family
ID=42165541
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011535656A Active JP5719301B2 (ja) | 2008-11-12 | 2009-11-05 | 血清不含インスリン不含で第ｖｉｉ因子を産生する方法 |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US20100120093A1 (https=) |
| EP (2) | EP2356247B2 (https=) |
| JP (1) | JP5719301B2 (https=) |
| AU (1) | AU2009314345B2 (https=) |
| CA (1) | CA2742107A1 (https=) |
| DK (1) | DK2356247T4 (https=) |
| ES (1) | ES2547881T5 (https=) |
| HK (1) | HK1219113A1 (https=) |
| HR (1) | HRP20151054T4 (https=) |
| HU (1) | HUE026210T2 (https=) |
| IL (1) | IL212355A (https=) |
| PL (1) | PL2356247T5 (https=) |
| PT (1) | PT2356247E (https=) |
| SI (1) | SI2356247T2 (https=) |
| WO (1) | WO2010056584A1 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013535981A (ja) * | 2010-08-20 | 2013-09-19 | ワイス・エルエルシー | 成長因子不含適合細胞の細胞培養 |
| US20120076779A1 (en) | 2010-09-17 | 2012-03-29 | Baxter Healthcare S.A. | STABILIZATION OF IMMUNOGLOBULINS AND OTHER PROTEINS THROUGH AQUEOUS FORMULATIONS WITH SODIUM CHLORIDE AT WEAK ACIDIC TO NEUTRAL ph |
| US9217168B2 (en) | 2013-03-14 | 2015-12-22 | Momenta Pharmaceuticals, Inc. | Methods of cell culture |
| AR095196A1 (es) | 2013-03-15 | 2015-09-30 | Regeneron Pharma | Medio de cultivo celular libre de suero |
| KR20150026024A (ko) * | 2013-08-30 | 2015-03-11 | 한미약품 주식회사 | 인간 혈액응고 7인자 유도체의 대량 생산 방법 |
| US11286292B2 (en) | 2014-02-07 | 2022-03-29 | Cmc Biologics A/S | Bioreactor arrangement and continuous process for producing and capturing a biopolymer |
| NZ724826A (en) * | 2014-04-16 | 2022-07-29 | Cmc Biologics As | A high cell density fill and draw fermentation process |
| AU2015269365A1 (en) | 2014-06-04 | 2016-12-15 | Amgen Inc. | Methods for harvesting mammalian cell cultures |
| US12297451B1 (en) | 2019-10-25 | 2025-05-13 | Regeneron Pharmaceuticals, Inc. | Cell culture medium |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4456591A (en) | 1981-06-25 | 1984-06-26 | Baxter Travenol Laboratories, Inc. | Therapeutic method for activating factor VII |
| GR860984B (en) | 1985-04-17 | 1986-08-18 | Zymogenetics Inc | Expression of factor vii and ix activities in mammalian cells |
| GB9022545D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| US5997864A (en) | 1995-06-07 | 1999-12-07 | Novo Nordisk A/S | Modified factor VII |
| US5804420A (en) * | 1997-04-18 | 1998-09-08 | Bayer Corporation | Preparation of recombinant Factor VIII in a protein free medium |
| AT407255B (de) * | 1997-06-20 | 2001-02-26 | Immuno Ag | Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons |
| AT409379B (de) † | 1999-06-02 | 2002-07-25 | Baxter Ag | Medium zur protein- und serumfreien kultivierung von zellen |
| DK1200561T3 (da) † | 1999-08-05 | 2006-10-16 | Baxter Ag | Rekombinant stabil celleklon, dens fremstilling og anvendelse |
| US20030204338A1 (en) | 2002-04-22 | 2003-10-30 | Peter Martinek | Method and measurement probe for the performance of measurements in water supply systems |
| US20040185535A1 (en) † | 2003-03-21 | 2004-09-23 | Giles Wilson | Industrial-scale serum-free production of recombinant FVII in mammalian cells |
| BR0114373A (pt) * | 2000-10-02 | 2004-02-17 | Novo Nordisk As | Célula hospedeira eucariótica, métodos para a produção de fvii e de fviia ou de suas variantes, de uma proteìna dependente de vitamina k e de uma célula hospedeira eucariótica produtora de fvii ou de seu análogo, vetor recombinante, fvii e fviia recombinante ou suas variantes, e, proteìna dependente de vitamina k recombinante |
| EP1434857B1 (en) * | 2001-10-02 | 2007-08-01 | Novo Nordisk Health Care AG | Method for production of recombinant proteins in eukaryote cells |
| US20050214289A1 (en) * | 2004-03-26 | 2005-09-29 | Human Genome Sciences, Inc. | Antibodies against Nogo receptor |
| US20060094104A1 (en) | 2004-10-29 | 2006-05-04 | Leopold Grillberger | Animal protein-free media for cultivation of cells |
| EP1851305B1 (en) * | 2005-02-11 | 2012-01-18 | Novo Nordisk Health Care AG | Production of a polypeptide in a serum-free cell culture liquid containing plant protein hydrolysate |
| CN101300342A (zh) † | 2005-11-02 | 2008-11-05 | 惠氏公司 | 用于使哺乳动物细胞具备适应性的方法 |
| ES2474573T3 (es) † | 2006-01-04 | 2014-07-09 | Baxter International Inc | Medio de cultivo celular sin oligop�ptidos |
-
2009
- 2009-11-05 PT PT98265770T patent/PT2356247E/pt unknown
- 2009-11-05 WO PCT/US2009/063338 patent/WO2010056584A1/en not_active Ceased
- 2009-11-05 EP EP09826577.0A patent/EP2356247B2/en not_active Not-in-force
- 2009-11-05 JP JP2011535656A patent/JP5719301B2/ja active Active
- 2009-11-05 CA CA2742107A patent/CA2742107A1/en not_active Abandoned
- 2009-11-05 EP EP15169975.8A patent/EP2977461A1/en not_active Withdrawn
- 2009-11-05 ES ES09826577T patent/ES2547881T5/es active Active
- 2009-11-05 HU HUE09826577A patent/HUE026210T2/en unknown
- 2009-11-05 SI SI200931283T patent/SI2356247T2/sl unknown
- 2009-11-05 PL PL09826577T patent/PL2356247T5/pl unknown
- 2009-11-05 HR HRP20151054T patent/HRP20151054T4/hr unknown
- 2009-11-05 US US12/612,752 patent/US20100120093A1/en not_active Abandoned
- 2009-11-05 AU AU2009314345A patent/AU2009314345B2/en not_active Ceased
- 2009-11-05 DK DK09826577.0T patent/DK2356247T4/da active
-
2011
- 2011-04-14 IL IL212355A patent/IL212355A/en not_active IP Right Cessation
-
2016
- 2016-06-14 HK HK16106839.4A patent/HK1219113A1/en unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2012508562A5 (https=) | ||
| CN103205396B (zh) | 一种hek-293t细胞的悬浮驯化和无血清驯化方法 | |
| CN106987554B (zh) | 悬浮细胞株及其驯化方法 | |
| US11629338B2 (en) | Method for acclimating and suspending Vero and second order production process for virus | |
| CN105861422B (zh) | 一种适应无血清全悬浮培养的mdck细胞系的制备方法及通过该制备方法获得的mdck细胞系 | |
| CN105112352A (zh) | 一株适应全悬浮培养的st细胞及其应用和培养疫苗病毒的方法 | |
| JP5719301B2 (ja) | 血清不含インスリン不含で第ｖｉｉ因子を産生する方法 | |
| WO2024153174A1 (zh) | 一种适应无载体无血清悬浮培养的细胞驯化方法、干细胞系和应用 | |
| CN108570454A (zh) | Mdbk驯化悬浮方法和二阶病毒生产工艺 | |
| CN101831403B (zh) | 体外扩增人脐带胎盘间充质干细胞的方法 | |
| US20250129341A1 (en) | Establishment and suspension acclimation of crfk adherent cell line, and its application | |
| CN108570445A (zh) | Pk15细胞驯化悬浮方法和二阶病毒生产工艺 | |
| CN106282090B (zh) | 一种驯化后的cho-s细胞系及其培养方法与应用 | |
| CN114717182A (zh) | 一种山羊结肠类器官3d培养的分子培养基 | |
| CN104894054A (zh) | 一种猴胚胎肾上皮细胞Marc-145悬浮适应株及其在培养蓝耳病病毒、生产蓝耳病毒疫苗中的应用 | |
| KR100683429B1 (ko) | 생물학적 제제를 생산하기 위한 세포의 제조 | |
| CN119979470A (zh) | 一种可体外长期培养的猪肌肉干细胞细胞系及其应用 | |
| JP2859679B2 (ja) | 新規細胞株 | |
| CN112251397B (zh) | 一种新生猪胰岛细胞培养方法 | |
| CN108034634B (zh) | 一种从经血中分离宫内膜间充质干细胞的方法 | |
| CN117757722A (zh) | 使细胞驯化为无血清悬浮培养的方法 | |
| Huang et al. | Tissue culture investigations of bamboo V, Recovery of callus from protoplasts of suspension-cultured | |
| CN113106066A (zh) | 一种肉瘤细胞培养基以及采用该培养基体外生产Matrigel原液的方法 | |
| CN106479976A (zh) | 一种脐带血巨核祖细胞体外培养的方法 | |
| CN106834213B (zh) | 一种诱导多能干细胞形成毛乳头细胞的诱导培养基及诱导方法 |