CN108570454A - Mdbk驯化悬浮方法和二阶病毒生产工艺 - Google Patents
Mdbk驯化悬浮方法和二阶病毒生产工艺 Download PDFInfo
- Publication number
- CN108570454A CN108570454A CN201711098901.9A CN201711098901A CN108570454A CN 108570454 A CN108570454 A CN 108570454A CN 201711098901 A CN201711098901 A CN 201711098901A CN 108570454 A CN108570454 A CN 108570454A
- Authority
- CN
- China
- Prior art keywords
- cell
- mdbk
- suspension
- culture
- serum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000725 suspension Substances 0.000 title claims abstract description 131
- 238000000034 method Methods 0.000 title claims abstract description 69
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 56
- 241000700605 Viruses Species 0.000 title claims abstract description 47
- 230000008569 process Effects 0.000 title abstract description 16
- 239000001963 growth medium Substances 0.000 claims abstract description 88
- 210000002966 serum Anatomy 0.000 claims abstract description 78
- 230000001464 adherent effect Effects 0.000 claims abstract description 64
- 230000012010 growth Effects 0.000 claims abstract description 63
- 239000002574 poison Substances 0.000 claims abstract description 36
- 231100000614 poison Toxicity 0.000 claims abstract description 35
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 239000013587 production medium Substances 0.000 claims abstract description 20
- 238000010790 dilution Methods 0.000 claims abstract description 11
- 239000012895 dilution Substances 0.000 claims abstract description 11
- 230000003612 virological effect Effects 0.000 claims abstract description 10
- 230000010261 cell growth Effects 0.000 claims description 30
- 229960005486 vaccine Drugs 0.000 claims description 24
- 238000012549 training Methods 0.000 claims description 19
- 239000002054 inoculum Substances 0.000 claims description 15
- 229940030156 cell vaccine Drugs 0.000 claims description 11
- 238000003306 harvesting Methods 0.000 claims description 6
- 208000005562 infectious bovine rhinotracheitis Diseases 0.000 claims description 5
- 210000001015 abdomen Anatomy 0.000 claims 1
- 230000003834 intracellular effect Effects 0.000 claims 1
- 239000000203 mixture Substances 0.000 abstract description 16
- 239000000126 substance Substances 0.000 abstract description 16
- 238000004114 suspension culture Methods 0.000 abstract description 9
- 230000003902 lesion Effects 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 369
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 22
- 239000012888 bovine serum Substances 0.000 description 22
- 230000001079 digestive effect Effects 0.000 description 21
- 239000012679 serum free medium Substances 0.000 description 20
- 238000005516 engineering process Methods 0.000 description 19
- 230000004899 motility Effects 0.000 description 19
- 238000012546 transfer Methods 0.000 description 16
- 238000005119 centrifugation Methods 0.000 description 15
- 239000002609 medium Substances 0.000 description 15
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 14
- 241001465754 Metazoa Species 0.000 description 14
- 230000008859 change Effects 0.000 description 14
- 239000008103 glucose Substances 0.000 description 14
- 230000029087 digestion Effects 0.000 description 13
- 238000011084 recovery Methods 0.000 description 13
- 239000004615 ingredient Substances 0.000 description 12
- 229910052757 nitrogen Inorganic materials 0.000 description 11
- 239000006228 supernatant Substances 0.000 description 10
- 238000005070 sampling Methods 0.000 description 9
- 241000283690 Bos taurus Species 0.000 description 8
- 239000006143 cell culture medium Substances 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 238000001556 precipitation Methods 0.000 description 8
- 241000710780 Bovine viral diarrhea virus 1 Species 0.000 description 7
- 239000012530 fluid Substances 0.000 description 7
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 7
- 238000010899 nucleation Methods 0.000 description 7
- 230000003068 static effect Effects 0.000 description 7
- 206010012735 Diarrhoea Diseases 0.000 description 6
- 108010019160 Pancreatin Proteins 0.000 description 6
- 239000007789 gas Substances 0.000 description 6
- 238000011081 inoculation Methods 0.000 description 6
- 229940055695 pancreatin Drugs 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000004113 cell culture Methods 0.000 description 5
- 230000001413 cellular effect Effects 0.000 description 5
- 238000001802 infusion Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000012216 screening Methods 0.000 description 5
- 230000006978 adaptation Effects 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 230000003833 cell viability Effects 0.000 description 4
- 238000005138 cryopreservation Methods 0.000 description 4
- 238000012136 culture method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000003102 growth factor Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 230000035755 proliferation Effects 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 239000002356 single layer Substances 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 239000006185 dispersion Substances 0.000 description 3
- -1 element Substances 0.000 description 3
- 239000000413 hydrolysate Substances 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 230000001788 irregular Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 150000003722 vitamin derivatives Chemical class 0.000 description 3
- 206010013457 Dissociation Diseases 0.000 description 2
- 239000007640 basal medium Substances 0.000 description 2
- PPBOKXIGFIBOGK-BDTUAEFFSA-N bvdv Chemical compound C([C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)C(C)C)[C@@H](C)CC)C1=CN=CN1 PPBOKXIGFIBOGK-BDTUAEFFSA-N 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- 208000018459 dissociative disease Diseases 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000012239 gene modification Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 230000005017 genetic modification Effects 0.000 description 2
- 235000013617 genetically modified food Nutrition 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 238000010257 thawing Methods 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000001524 infective effect Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
- 239000004017 serum-free culture medium Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002435 venom Substances 0.000 description 1
- 231100000611 venom Toxicity 0.000 description 1
- 210000001048 venom Anatomy 0.000 description 1
- 229960004854 viral vaccine Drugs 0.000 description 1
- 239000005723 virus inoculator Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16051—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24311—Pestivirus, e.g. bovine viral diarrhea virus
- C12N2770/24334—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24311—Pestivirus, e.g. bovine viral diarrhea virus
- C12N2770/24351—Methods of production or purification of viral material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711098901.9A CN108570454A (zh) | 2017-11-09 | 2017-11-09 | Mdbk驯化悬浮方法和二阶病毒生产工艺 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711098901.9A CN108570454A (zh) | 2017-11-09 | 2017-11-09 | Mdbk驯化悬浮方法和二阶病毒生产工艺 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN108570454A true CN108570454A (zh) | 2018-09-25 |
Family
ID=63576500
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201711098901.9A Pending CN108570454A (zh) | 2017-11-09 | 2017-11-09 | Mdbk驯化悬浮方法和二阶病毒生产工艺 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108570454A (zh) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109207439A (zh) * | 2018-10-09 | 2019-01-15 | 华农(肇庆)生物产业技术研究院有限公司 | 一种鸭瘟病毒的全悬浮培养方法 |
CN110241090A (zh) * | 2019-05-07 | 2019-09-17 | 江苏南农高科技股份有限公司 | 一种全悬浮细胞培养生产猪伪狂犬病毒抗原的方法 |
CN110713971A (zh) * | 2019-11-06 | 2020-01-21 | 深圳市菲鹏生物治疗股份有限公司 | 一种无血清悬浮培养型293t细胞、其制备方法以及病毒的包装方法 |
CN111671893A (zh) * | 2020-07-31 | 2020-09-18 | 中国农业大学 | 牛传染性鼻气管炎病毒与牛支原体二联灭活疫苗及其制备方法与所用悬浮mdbk细胞 |
CN116355857A (zh) * | 2023-05-10 | 2023-06-30 | 北京华夏兴洋生物科技有限公司 | 悬浮培养的牛肾细胞及其制备方法与应用 |
CN116970547A (zh) * | 2023-02-24 | 2023-10-31 | 广州恩宝生物医药科技有限公司 | 一种驯化无血清全悬浮hek293细胞的方法及其应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050118698A1 (en) * | 2001-09-12 | 2005-06-02 | Chiron Behring Gmbh & Co. | Multiplication of viruses in a cell culture |
CN104436184A (zh) * | 2014-11-25 | 2015-03-25 | 成都威尔诺生物科技有限公司 | 流感病毒疫苗的细胞生产方法 |
CN107201333A (zh) * | 2016-03-16 | 2017-09-26 | 金宇保灵生物药品有限公司 | 可悬浮培养的牛肾细胞在病毒培养及疫苗生产的应用 |
-
2017
- 2017-11-09 CN CN201711098901.9A patent/CN108570454A/zh active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050118698A1 (en) * | 2001-09-12 | 2005-06-02 | Chiron Behring Gmbh & Co. | Multiplication of viruses in a cell culture |
CN104436184A (zh) * | 2014-11-25 | 2015-03-25 | 成都威尔诺生物科技有限公司 | 流感病毒疫苗的细胞生产方法 |
CN107201333A (zh) * | 2016-03-16 | 2017-09-26 | 金宇保灵生物药品有限公司 | 可悬浮培养的牛肾细胞在病毒培养及疫苗生产的应用 |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109207439A (zh) * | 2018-10-09 | 2019-01-15 | 华农(肇庆)生物产业技术研究院有限公司 | 一种鸭瘟病毒的全悬浮培养方法 |
CN110241090A (zh) * | 2019-05-07 | 2019-09-17 | 江苏南农高科技股份有限公司 | 一种全悬浮细胞培养生产猪伪狂犬病毒抗原的方法 |
CN110241090B (zh) * | 2019-05-07 | 2023-10-13 | 江苏南农高科技股份有限公司 | 一种全悬浮细胞培养生产猪伪狂犬病毒抗原的方法 |
CN110713971A (zh) * | 2019-11-06 | 2020-01-21 | 深圳市菲鹏生物治疗股份有限公司 | 一种无血清悬浮培养型293t细胞、其制备方法以及病毒的包装方法 |
CN111671893A (zh) * | 2020-07-31 | 2020-09-18 | 中国农业大学 | 牛传染性鼻气管炎病毒与牛支原体二联灭活疫苗及其制备方法与所用悬浮mdbk细胞 |
CN111671893B (zh) * | 2020-07-31 | 2022-07-19 | 中国农业大学 | 牛传染性鼻气管炎病毒与牛支原体二联灭活疫苗及其制备方法与所用悬浮mdbk细胞 |
CN116970547A (zh) * | 2023-02-24 | 2023-10-31 | 广州恩宝生物医药科技有限公司 | 一种驯化无血清全悬浮hek293细胞的方法及其应用 |
CN116970547B (zh) * | 2023-02-24 | 2024-04-09 | 广州恩宝生物医药科技有限公司 | 一种驯化无血清全悬浮hek293细胞的方法及其应用 |
CN116355857A (zh) * | 2023-05-10 | 2023-06-30 | 北京华夏兴洋生物科技有限公司 | 悬浮培养的牛肾细胞及其制备方法与应用 |
CN116355857B (zh) * | 2023-05-10 | 2023-09-12 | 北京华夏兴洋生物科技有限公司 | 悬浮培养的牛肾细胞及其制备方法与应用 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108570454A (zh) | Mdbk驯化悬浮方法和二阶病毒生产工艺 | |
US11629338B2 (en) | Method for acclimating and suspending Vero and second order production process for virus | |
CN103205396B (zh) | 一种hek-293t细胞的悬浮驯化和无血清驯化方法 | |
CN107201334B (zh) | 能悬浮培养的牛肾细胞及其悬浮培养方法与应用 | |
CN101979518B (zh) | 一种制备伪狂犬病病毒的方法 | |
CN108570445A (zh) | Pk15细胞驯化悬浮方法和二阶病毒生产工艺 | |
CN102559617A (zh) | 生物反应器微载体培养人二倍体细胞生产病毒疫苗的方法 | |
CN108396006A (zh) | 一种bhk-21细胞株及其培养方法和用途 | |
CN113249308B (zh) | 花鲈动脉球细胞系及其应用和培养方法 | |
CN105316279A (zh) | 一种高效分离纯化乳腺上皮细胞的方法 | |
CN110373379A (zh) | 一种Marc-145全悬浮细胞直接培养猪蓝耳病病毒疫苗的方法 | |
CN117025551A (zh) | 一种牛结节性皮肤病病毒毒株、以及由其制备的灭活疫苗和疫苗的制备方法 | |
CN109913404A (zh) | 鸡传染性法氏囊病毒活疫苗的制备方法 | |
CN114276981B (zh) | 一种适应猪流行性腹泻病毒的Vero-E6悬浮细胞株sVero-E6及其应用 | |
CN104894054B (zh) | 一种猴胚胎肾上皮细胞Marc‑145悬浮适应株及其在培养蓝耳病病毒、生产蓝耳病毒疫苗中的应用 | |
CN104593335B (zh) | 低血清培养Marc‑145细胞生产猪繁殖与呼吸道综合征CH‑1R株病毒的方法 | |
CN110241090B (zh) | 一种全悬浮细胞培养生产猪伪狂犬病毒抗原的方法 | |
CN109679925A (zh) | 鸭坦布苏病毒液的制备方法及其产品 | |
CN109609436A (zh) | 一种无co2环境的mdck细胞全悬浮无血清细胞系的制备方法 | |
CN116218763A (zh) | 一种猪流行性腹泻病毒株的全悬浮细胞培养方法 | |
CN114276982B (zh) | 一种适应猪流行性腹泻病毒的st悬浮细胞株st-j及其应用 | |
CN104740627B (zh) | 一种兽用伪狂犬弱毒活疫苗的规模化生产方法 | |
CN105176915A (zh) | 一种适于Marc-145细胞生长的低血清无蛋白培养基及其制备方法 | |
CN108570444A (zh) | St细胞驯化悬浮方法和二阶病毒生产工艺 | |
CN104630159A (zh) | 低血清培养st细胞生产猪瘟c株病毒的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20190911 Address after: 730070 No. 70, Jiuzhou West Road, Anning District, Lanzhou, Gansu Applicant after: JIANSHUN BIOSCIENCES Co.,Ltd. Applicant after: Aoskang Biopharmaceutical (Haimen) Co.,Ltd. Address before: 730070 No. 70, Jiuzhou West Road, Anning District, Lanzhou, Gansu Applicant before: JIANSHUN BIOSCIENCES Co.,Ltd. |
|
TA01 | Transfer of patent application right | ||
CB02 | Change of applicant information |
Address after: 730070 No. 70, Jiuzhou West Road, Anning District, Lanzhou, Gansu Applicant after: JIANSHUN BIOSCIENCES Co.,Ltd. Applicant after: Auscon biopharmaceutical (Nantong) Co.,Ltd. Address before: 730070 No. 70, Jiuzhou West Road, Anning District, Lanzhou, Gansu Applicant before: JIANSHUN BIOSCIENCES Co.,Ltd. Applicant before: Aoskang Biopharmaceutical (Haimen) Co.,Ltd. |
|
CB02 | Change of applicant information | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20210812 Address after: 201203 units F, G, h, I, J and K, floor 1, building 3, Lane 100, Zuchongzhi Road, China (Shanghai) pilot Free Trade Zone, Pudong New Area, Shanghai Applicant after: Shanghai jianshibai Biotechnology Co.,Ltd. Applicant after: JIANSHUN BIOSCIENCES Co.,Ltd. Applicant after: Auscon biopharmaceutical (Nantong) Co.,Ltd. Address before: 730070 No. 70, Jiuzhou Tongxi Road, Anning District, Lanzhou City, Gansu Province Applicant before: JIANSHUN BIOSCIENCES Co.,Ltd. Applicant before: Auscon biopharmaceutical (Nantong) Co.,Ltd. |
|
TA01 | Transfer of patent application right | ||
CB02 | Change of applicant information |
Address after: 201203 units F, G, h, I, J and K, floor 1, building 3, Lane 100, Zuchongzhi Road, China (Shanghai) pilot Free Trade Zone, Pudong New Area, Shanghai Applicant after: Shanghai jianshibai Biotechnology Co.,Ltd. Applicant after: JIANSHUN BIOSCIENCES Co.,Ltd. Applicant after: Aosikang Biology (Nantong) Co.,Ltd. Address before: 201203 units F, G, h, I, J and K, floor 1, building 3, Lane 100, Zuchongzhi Road, China (Shanghai) pilot Free Trade Zone, Pudong New Area, Shanghai Applicant before: Shanghai jianshibai Biotechnology Co.,Ltd. Applicant before: JIANSHUN BIOSCIENCES Co.,Ltd. Applicant before: Auscon biopharmaceutical (Nantong) Co.,Ltd. |
|
CB02 | Change of applicant information |