JP2012506710A5 - - Google Patents
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- JP2012506710A5 JP2012506710A5 JP2011533616A JP2011533616A JP2012506710A5 JP 2012506710 A5 JP2012506710 A5 JP 2012506710A5 JP 2011533616 A JP2011533616 A JP 2011533616A JP 2011533616 A JP2011533616 A JP 2011533616A JP 2012506710 A5 JP2012506710 A5 JP 2012506710A5
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- 230000000694 effects Effects 0.000 claims 44
- 210000004027 cell Anatomy 0.000 claims 31
- 102000039446 nucleic acids Human genes 0.000 claims 13
- 108020004707 nucleic acids Proteins 0.000 claims 13
- 150000007523 nucleic acids Chemical class 0.000 claims 13
- 102000003886 Glycoproteins Human genes 0.000 claims 11
- 108090000288 Glycoproteins Proteins 0.000 claims 11
- 101150104887 ALG11 gene Proteins 0.000 claims 9
- 108010089072 Dolichyl-diphosphooligosaccharide-protein glycotransferase Proteins 0.000 claims 9
- 101001010097 Shigella phage SfV Bactoprenol-linked glucose translocase Proteins 0.000 claims 8
- 125000000311 mannosyl group Chemical group C1([C@@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims 8
- 108090000623 proteins and genes Proteins 0.000 claims 8
- 150000002632 lipids Chemical class 0.000 claims 5
- 150000002772 monosaccharides Chemical class 0.000 claims 4
- 229920001542 oligosaccharide Polymers 0.000 claims 4
- 150000002482 oligosaccharides Chemical class 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 4
- 101100322982 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) alg-11 gene Proteins 0.000 claims 3
- 239000001963 growth medium Substances 0.000 claims 3
- 238000004519 manufacturing process Methods 0.000 claims 3
- 101150103561 ALG2 gene Proteins 0.000 claims 2
- 101150039297 Alg3 gene Proteins 0.000 claims 2
- 102100022622 Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase Human genes 0.000 claims 2
- 241000222722 Leishmania <genus> Species 0.000 claims 2
- 108010087568 Mannosyltransferases Proteins 0.000 claims 2
- 102000006722 Mannosyltransferases Human genes 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 101150089325 dpm1 gene Proteins 0.000 claims 2
- 210000003527 eukaryotic cell Anatomy 0.000 claims 2
- 210000004962 mammalian cell Anatomy 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 102100021266 Alpha-(1,6)-fucosyltransferase Human genes 0.000 claims 1
- 101710146120 Alpha-(1,6)-fucosyltransferase Proteins 0.000 claims 1
- 108010002020 Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase Proteins 0.000 claims 1
- 102100024296 Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase Human genes 0.000 claims 1
- 101710122990 Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase Proteins 0.000 claims 1
- 102100037982 Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A Human genes 0.000 claims 1
- 101710170638 Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A Proteins 0.000 claims 1
- 102100026349 Beta-1,4-galactosyltransferase 1 Human genes 0.000 claims 1
- 102100024775 Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase Human genes 0.000 claims 1
- 108030001771 Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferases Proteins 0.000 claims 1
- TXCIAUNLDRJGJZ-UHFFFAOYSA-N CMP-N-acetyl neuraminic acid Natural products O1C(C(O)C(O)CO)C(NC(=O)C)C(O)CC1(C(O)=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(N=C(N)C=C2)=O)O1 TXCIAUNLDRJGJZ-UHFFFAOYSA-N 0.000 claims 1
- TXCIAUNLDRJGJZ-BILDWYJOSA-N CMP-N-acetyl-beta-neuraminic acid Chemical compound O1[C@@H]([C@H](O)[C@H](O)CO)[C@H](NC(=O)C)[C@@H](O)C[C@]1(C(O)=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(N=C(N)C=C2)=O)O1 TXCIAUNLDRJGJZ-BILDWYJOSA-N 0.000 claims 1
- 102100020746 Dolichol-phosphate mannosyltransferase subunit 1 Human genes 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 108010019236 Fucosyltransferases Proteins 0.000 claims 1
- 102000006471 Fucosyltransferases Human genes 0.000 claims 1
- LQEBEXMHBLQMDB-UHFFFAOYSA-N GDP-L-fucose Natural products OC1C(O)C(O)C(C)OC1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C3=C(C(N=C(N)N3)=O)N=C2)O1 LQEBEXMHBLQMDB-UHFFFAOYSA-N 0.000 claims 1
- 102100024515 GDP-L-fucose synthase Human genes 0.000 claims 1
- 101710119007 GDP-L-fucose synthase Proteins 0.000 claims 1
- LQEBEXMHBLQMDB-JGQUBWHWSA-N GDP-beta-L-fucose Chemical compound O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C3=C(C(NC(N)=N3)=O)N=C2)O1 LQEBEXMHBLQMDB-JGQUBWHWSA-N 0.000 claims 1
- 108010062427 GDP-mannose 4,6-dehydratase Proteins 0.000 claims 1
- 102000002312 GDPmannose 4,6-dehydratase Human genes 0.000 claims 1
- KUYCTNQKTFGPMI-SXHURMOUSA-N Glc(a1-2)Glc(a1-3)Glc(a1-3)Man(a1-2)Man(a1-2)Man(a1-3)[Man(a1-2)Man(a1-3)[Man(a1-2)Man(a1-6)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc Chemical compound O[C@@H]1[C@@H](NC(=O)C)C(O)O[C@H](CO)[C@H]1O[C@H]1[C@H](NC(C)=O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O[C@@H]3[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O[C@@H]3[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O[C@@H]3[C@H]([C@@H](O[C@@H]4[C@@H]([C@@H](O[C@@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O[C@@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)[C@H](O)[C@@H](CO)O4)O)[C@H](O)[C@@H](CO)O3)O)[C@H](O)[C@@H](CO[C@@H]3[C@H]([C@@H](O[C@@H]4[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O4)O[C@@H]4[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O4)O)[C@H](O)[C@@H](CO[C@@H]4[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O4)O[C@@H]4[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O4)O)O3)O)O2)O)[C@@H](CO)O1 KUYCTNQKTFGPMI-SXHURMOUSA-N 0.000 claims 1
- 108010055629 Glucosyltransferases Proteins 0.000 claims 1
- 102000000340 Glucosyltransferases Human genes 0.000 claims 1
- 108700023372 Glycosyltransferases Proteins 0.000 claims 1
- 102000051366 Glycosyltransferases Human genes 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 claims 1
- 101000972916 Homo sapiens Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase Proteins 0.000 claims 1
- 101000932202 Homo sapiens Dolichol-phosphate mannosyltransferase subunit 1 Proteins 0.000 claims 1
- 101000588377 Homo sapiens N-acylneuraminate cytidylyltransferase Proteins 0.000 claims 1
- 241000222740 Leishmania braziliensis Species 0.000 claims 1
- 241000222727 Leishmania donovani Species 0.000 claims 1
- 241000222696 Leishmania guyanensis Species 0.000 claims 1
- 241000222697 Leishmania infantum Species 0.000 claims 1
- 241000222732 Leishmania major Species 0.000 claims 1
- 241000222734 Leishmania mexicana Species 0.000 claims 1
- 241000222736 Leishmania tropica Species 0.000 claims 1
- ZTOKCBJDEGPICW-UHFFFAOYSA-N Man3GlcNAc2 Natural products OC1C(NC(=O)C)C(O)OC(CO)C1OC1C(NC(C)=O)C(O)C(OC2C(C(OC3C(C(O)C(O)C(CO)O3)O)C(O)C(COC3C(C(O)C(O)C(CO)O3)O)O2)O)C(CO)O1 ZTOKCBJDEGPICW-UHFFFAOYSA-N 0.000 claims 1
- 101100192157 Mus musculus Psen2 gene Proteins 0.000 claims 1
- 108010035265 N-acetylneuraminate synthase Proteins 0.000 claims 1
- 102100031349 N-acylneuraminate cytidylyltransferase Human genes 0.000 claims 1
- 102100039306 Nucleotide pyrophosphatase Human genes 0.000 claims 1
- 229910019142 PO4 Inorganic materials 0.000 claims 1
- 101150011873 Pdcd6 gene Proteins 0.000 claims 1
- 102100029954 Sialic acid synthase Human genes 0.000 claims 1
- 241000223997 Toxoplasma gondii Species 0.000 claims 1
- 241000223105 Trypanosoma brucei Species 0.000 claims 1
- 241000223109 Trypanosoma cruzi Species 0.000 claims 1
- LFTYTUAZOPRMMI-CFRASDGPSA-N UDP-N-acetyl-alpha-D-glucosamine Chemical compound O1[C@H](CO)[C@@H](O)[C@H](O)[C@@H](NC(=O)C)[C@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(=O)C=C2)=O)O1 LFTYTUAZOPRMMI-CFRASDGPSA-N 0.000 claims 1
- 101710091363 UDP-N-acetylglucosamine 2-epimerase Proteins 0.000 claims 1
- LFTYTUAZOPRMMI-UHFFFAOYSA-N UNPD164450 Natural products O1C(CO)C(O)C(O)C(NC(=O)C)C1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(NC(=O)C=C2)=O)O1 LFTYTUAZOPRMMI-UHFFFAOYSA-N 0.000 claims 1
- HSCJRCZFDFQWRP-UHFFFAOYSA-N Uridindiphosphoglukose Natural products OC1C(O)C(O)C(CO)OC1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-UHFFFAOYSA-N 0.000 claims 1
- USAZACJQJDHAJH-KDEXOMDGSA-N [[(2r,3s,4r,5s)-5-(2,4-dioxo-1h-pyrimidin-6-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] hydrogen phosphate Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](C=2NC(=O)NC(=O)C=2)O1 USAZACJQJDHAJH-KDEXOMDGSA-N 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- ZTOKCBJDEGPICW-GWPISINRSA-N alpha-D-Manp-(1->3)-[alpha-D-Manp-(1->6)]-beta-D-Manp-(1->4)-beta-D-GlcpNAc-(1->4)-beta-D-GlcpNAc Chemical compound O[C@@H]1[C@@H](NC(=O)C)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](NC(C)=O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O[C@@H]3[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)[C@H](O)[C@@H](CO[C@@H]3[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)O2)O)[C@@H](CO)O1 ZTOKCBJDEGPICW-GWPISINRSA-N 0.000 claims 1
- 108010064886 beta-D-galactoside alpha 2-6-sialyltransferase Proteins 0.000 claims 1
- 108010044698 beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase Proteins 0.000 claims 1
- 230000003197 catalytic effect Effects 0.000 claims 1
- 230000001086 cytosolic effect Effects 0.000 claims 1
- 230000003292 diminished effect Effects 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 230000002538 fungal effect Effects 0.000 claims 1
- 230000003834 intracellular effect Effects 0.000 claims 1
- 230000004576 lipid-binding Effects 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- KSEVLWYNDHVCJB-DMZKWVAKSA-N n-[(2r,3r,4r,5s,6r)-2-[(2r,3r,4s,5r)-2-acetamido-6-hydroxy-1-oxo-4-[(2s,3s,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-5-[(2r,3s,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyhexan-3-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl Chemical compound O([C@H]([C@H](C=O)NC(=O)C)[C@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H](CO)O[C@@H]1[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1NC(C)=O KSEVLWYNDHVCJB-DMZKWVAKSA-N 0.000 claims 1
- 108010067588 nucleotide pyrophosphatase Proteins 0.000 claims 1
- 210000000056 organ Anatomy 0.000 claims 1
- 239000010452 phosphate Substances 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US19802308P | 2008-10-31 | 2008-10-31 | |
| US61/198,023 | 2008-10-31 | ||
| EP08400044.7 | 2008-10-31 | ||
| EP08400044 | 2008-10-31 | ||
| PCT/EP2009/007816 WO2010049177A1 (en) | 2008-10-31 | 2009-10-31 | Novel tools for the production of glycosylated proteins in host cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2012506710A JP2012506710A (ja) | 2012-03-22 |
| JP2012506710A5 true JP2012506710A5 (https=) | 2012-11-08 |
Family
ID=40386055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011533616A Withdrawn JP2012506710A (ja) | 2008-10-31 | 2009-10-31 | 宿主細胞中での糖鎖付加タンパク質の産生のための新規ツール |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20110207214A1 (https=) |
| EP (1) | EP2350123A1 (https=) |
| JP (1) | JP2012506710A (https=) |
| CN (1) | CN102203123A (https=) |
| AU (1) | AU2009309925A1 (https=) |
| CA (1) | CA2741964A1 (https=) |
| IL (1) | IL212580A0 (https=) |
| TW (1) | TW201028431A (https=) |
| WO (1) | WO2010049177A1 (https=) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2252261T3 (es) * | 2000-06-28 | 2006-05-16 | Glycofi, Inc. | Metodos para producir glicoproteinas modificadas. |
| RU2012140429A (ru) | 2010-02-24 | 2014-03-27 | Мерк Шарп Энд Домэ Корп. | Способ увеличения занятости участков n-ликозилирования на терапевтических гликопротеинах, продуцируемых в p pastoris |
| EP2643456B1 (en) * | 2010-11-24 | 2016-05-11 | Novartis International Pharmaceutical Ltd. | Fusion enzymes having n-acetylglucosaminyltransferase activity |
| CN102586316B (zh) * | 2012-02-16 | 2013-08-07 | 中国科学院微生物研究所 | 人源糖基化改造的汉逊酵母 |
| CN104105789A (zh) * | 2012-02-16 | 2014-10-15 | 默沙东公司 | 降低酵母中甘露糖基转移酶活性的方法 |
| CN102839157A (zh) * | 2012-07-12 | 2012-12-26 | 扬州大学 | 稳定共表达hST3GalIV和β1-4GalT1的MDCK细胞系及构建方法和应用 |
| JP6356229B2 (ja) * | 2013-06-04 | 2018-07-11 | アイコン ジェネティクス ゲーエムベーハー | 植物産生糖タンパク質及び組換え糖タンパク質のｎ−グリコシル化部位占有率を調節する方法 |
| CA2916899C (en) * | 2013-07-10 | 2020-03-10 | Novartis Ag | Production of glycoproteins having increased n-glycosylation site occupancy |
| WO2015116315A1 (en) * | 2014-01-29 | 2015-08-06 | Amgen Inc. | Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins |
| SG11201700446XA (en) | 2014-07-21 | 2017-02-27 | Glykos Finland Oy | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
| CN105372214B (zh) * | 2014-08-18 | 2019-06-28 | 中国科学院上海有机化学研究所 | 一种鉴定新红细胞生成刺激蛋白的n-连接寡糖结构的方法 |
| CN104784754B (zh) * | 2015-03-04 | 2017-05-10 | 重庆市畜牧科学院 | 一种蚕丝人工血管及制备方法 |
| CN106867966B (zh) * | 2015-12-11 | 2020-11-24 | 复旦大学 | 稳定表达核心α(1,3)岩藻糖基转移酶的脊椎动物细胞系及其制备方法 |
| JP6757341B2 (ja) * | 2018-01-26 | 2020-09-16 | 東洋紡株式会社 | アスペルギルス属微生物が生産するタンパク質のｎ型糖鎖構造を改変する方法 |
| CN108179160B (zh) * | 2018-01-29 | 2021-05-11 | 江南大学 | 一种植烷醇连接的高甘露糖型寡糖的制备方法 |
| EP3788164A4 (en) * | 2018-05-01 | 2022-01-26 | Conagen Inc. | RECOMBINANT ORGANISMS AND METHODS FOR PRODUCTION OF HIGH GLYCAN OCCUPANCY GLYCOMOLECULES |
| CN110687035A (zh) * | 2019-10-10 | 2020-01-14 | 沈阳万类生物科技有限公司 | Annexin V-Light 650凋亡检测试剂盒 |
| WO2022190022A1 (en) * | 2021-03-11 | 2022-09-15 | Dyadic International (Usa), Inc. | Genetically-modified filamentous fungi for production of exogenous proteins having reduced or no n-linked glycosylation |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7507573B2 (en) * | 2003-11-14 | 2009-03-24 | Vib, Vzw | Modification of protein glycosylation in methylotrophic yeast |
| AU2005269759A1 (en) * | 2004-07-21 | 2006-02-09 | Glycofi, Inc. | Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform |
-
2009
- 2009-10-30 TW TW098136974A patent/TW201028431A/zh unknown
- 2009-10-31 WO PCT/EP2009/007816 patent/WO2010049177A1/en not_active Ceased
- 2009-10-31 CA CA2741964A patent/CA2741964A1/en not_active Abandoned
- 2009-10-31 US US13/126,856 patent/US20110207214A1/en not_active Abandoned
- 2009-10-31 CN CN2009801439193A patent/CN102203123A/zh active Pending
- 2009-10-31 AU AU2009309925A patent/AU2009309925A1/en not_active Abandoned
- 2009-10-31 EP EP09744637A patent/EP2350123A1/en not_active Withdrawn
- 2009-10-31 JP JP2011533616A patent/JP2012506710A/ja not_active Withdrawn
-
2011
- 2011-04-28 IL IL212580A patent/IL212580A0/en unknown
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