CN102203123A - 在宿主细胞中制造糖基化蛋白的新方法 - Google Patents

在宿主细胞中制造糖基化蛋白的新方法 Download PDF

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Publication number
CN102203123A
CN102203123A CN2009801439193A CN200980143919A CN102203123A CN 102203123 A CN102203123 A CN 102203123A CN 2009801439193 A CN2009801439193 A CN 2009801439193A CN 200980143919 A CN200980143919 A CN 200980143919A CN 102203123 A CN102203123 A CN 102203123A
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cell
glycoprotein
activity
type
cells
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Chinese (zh)
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乔尼·赫勒纽斯
克里斯廷·纽珀特
马库斯·艾比
法劳西·帕尔赛-纳赛比
亚历山大·丹尼尔·弗雷
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Lonza AG
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Lonza AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99018—Dolichyl-diphosphooligosaccharide—protein glycotransferase (2.4.99.18)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
CN2009801439193A 2008-10-31 2009-10-31 在宿主细胞中制造糖基化蛋白的新方法 Pending CN102203123A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US19802308P 2008-10-31 2008-10-31
US61/198,023 2008-10-31
EP08400044.7 2008-10-31
EP08400044 2008-10-31
PCT/EP2009/007816 WO2010049177A1 (en) 2008-10-31 2009-10-31 Novel tools for the production of glycosylated proteins in host cells

Publications (1)

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CN102203123A true CN102203123A (zh) 2011-09-28

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CN2009801439193A Pending CN102203123A (zh) 2008-10-31 2009-10-31 在宿主细胞中制造糖基化蛋白的新方法

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US (1) US20110207214A1 (https=)
EP (1) EP2350123A1 (https=)
JP (1) JP2012506710A (https=)
CN (1) CN102203123A (https=)
AU (1) AU2009309925A1 (https=)
CA (1) CA2741964A1 (https=)
IL (1) IL212580A0 (https=)
TW (1) TW201028431A (https=)
WO (1) WO2010049177A1 (https=)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102586316A (zh) * 2012-02-16 2012-07-18 中国科学院微生物研究所 人源糖基化改造的汉逊酵母
CN102839157A (zh) * 2012-07-12 2012-12-26 扬州大学 稳定共表达hST3GalIV和β1-4GalT1的MDCK细胞系及构建方法和应用
CN104784754A (zh) * 2015-03-04 2015-07-22 重庆市畜牧科学院 一种蚕丝人工血管及制备方法
CN105372214A (zh) * 2014-08-18 2016-03-02 中国科学院上海有机化学研究所 一种鉴定新红细胞生成刺激蛋白的n-连接寡糖结构的方法
CN106867966A (zh) * 2015-12-11 2017-06-20 复旦大学 稳定表达核心α(1,3)岩藻糖基转移酶的脊椎动物细胞系及其制备方法
CN108179160A (zh) * 2018-01-29 2018-06-19 江南大学 一种植烷醇连接的高甘露糖型寡糖的制备方法
CN110687035A (zh) * 2019-10-10 2020-01-14 沈阳万类生物科技有限公司 Annexin V-Light 650凋亡检测试剂盒
CN112074608A (zh) * 2018-05-01 2020-12-11 科纳根公司 用于产生具有高聚糖占有率的糖分子的重组生物体和方法

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2252261T3 (es) * 2000-06-28 2006-05-16 Glycofi, Inc. Metodos para producir glicoproteinas modificadas.
RU2012140429A (ru) 2010-02-24 2014-03-27 Мерк Шарп Энд Домэ Корп. Способ увеличения занятости участков n-ликозилирования на терапевтических гликопротеинах, продуцируемых в p pastoris
EP2643456B1 (en) * 2010-11-24 2016-05-11 Novartis International Pharmaceutical Ltd. Fusion enzymes having n-acetylglucosaminyltransferase activity
CN104105789A (zh) * 2012-02-16 2014-10-15 默沙东公司 降低酵母中甘露糖基转移酶活性的方法
JP6356229B2 (ja) * 2013-06-04 2018-07-11 アイコン ジェネティクス ゲーエムベーハー 植物産生糖タンパク質及び組換え糖タンパク質のｎ−グリコシル化部位占有率を調節する方法
CA2916899C (en) * 2013-07-10 2020-03-10 Novartis Ag Production of glycoproteins having increased n-glycosylation site occupancy
WO2015116315A1 (en) * 2014-01-29 2015-08-06 Amgen Inc. Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
JP6757341B2 (ja) * 2018-01-26 2020-09-16 東洋紡株式会社 アスペルギルス属微生物が生産するタンパク質のｎ型糖鎖構造を改変する方法
WO2022190022A1 (en) * 2021-03-11 2022-09-15 Dyadic International (Usa), Inc. Genetically-modified filamentous fungi for production of exogenous proteins having reduced or no n-linked glycosylation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7507573B2 (en) * 2003-11-14 2009-03-24 Vib, Vzw Modification of protein glycosylation in methylotrophic yeast
AU2005269759A1 (en) * 2004-07-21 2006-02-09 Glycofi, Inc. Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FARNOUSH PARSAIE NASAB ET AL.: "All in One: Leishmania major STT3 Proteins Substitute for the Whole Oligosaccharyltransferase Complex in Saccharomyces cerevisiae", 《MOLECULAR BIOLOGY OF THE CELL》 *
JONNE HELENIUS ET AL.: "Translocation of lipid-linked oligosaccharides across the ER membrane requires Rft1 protein", 《NATURE》 *
MICHA A. HAEUPTLE ET AL.: "Human RFT1 Deficiency Leads to a Disorder of N-Linked Glycosylation", 《THE AMERICAN JOURNAL OF HUMAN GENETICS》 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102586316A (zh) * 2012-02-16 2012-07-18 中国科学院微生物研究所 人源糖基化改造的汉逊酵母
CN102839157A (zh) * 2012-07-12 2012-12-26 扬州大学 稳定共表达hST3GalIV和β1-4GalT1的MDCK细胞系及构建方法和应用
CN105372214A (zh) * 2014-08-18 2016-03-02 中国科学院上海有机化学研究所 一种鉴定新红细胞生成刺激蛋白的n-连接寡糖结构的方法
CN105372214B (zh) * 2014-08-18 2019-06-28 中国科学院上海有机化学研究所 一种鉴定新红细胞生成刺激蛋白的n-连接寡糖结构的方法
CN104784754A (zh) * 2015-03-04 2015-07-22 重庆市畜牧科学院 一种蚕丝人工血管及制备方法
CN104784754B (zh) * 2015-03-04 2017-05-10 重庆市畜牧科学院 一种蚕丝人工血管及制备方法
CN106867966A (zh) * 2015-12-11 2017-06-20 复旦大学 稳定表达核心α(1,3)岩藻糖基转移酶的脊椎动物细胞系及其制备方法
CN106867966B (zh) * 2015-12-11 2020-11-24 复旦大学 稳定表达核心α(1,3)岩藻糖基转移酶的脊椎动物细胞系及其制备方法
CN108179160A (zh) * 2018-01-29 2018-06-19 江南大学 一种植烷醇连接的高甘露糖型寡糖的制备方法
CN108179160B (zh) * 2018-01-29 2021-05-11 江南大学 一种植烷醇连接的高甘露糖型寡糖的制备方法
CN112074608A (zh) * 2018-05-01 2020-12-11 科纳根公司 用于产生具有高聚糖占有率的糖分子的重组生物体和方法
CN110687035A (zh) * 2019-10-10 2020-01-14 沈阳万类生物科技有限公司 Annexin V-Light 650凋亡检测试剂盒

Also Published As

Publication number Publication date
WO2010049177A1 (en) 2010-05-06
US20110207214A1 (en) 2011-08-25
CA2741964A1 (en) 2010-05-06
EP2350123A1 (en) 2011-08-03
JP2012506710A (ja) 2012-03-22
IL212580A0 (en) 2011-07-31
AU2009309925A1 (en) 2010-05-06
TW201028431A (en) 2010-08-01

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