JP2014500019A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2014500019A5 JP2014500019A5 JP2013540368A JP2013540368A JP2014500019A5 JP 2014500019 A5 JP2014500019 A5 JP 2014500019A5 JP 2013540368 A JP2013540368 A JP 2013540368A JP 2013540368 A JP2013540368 A JP 2013540368A JP 2014500019 A5 JP2014500019 A5 JP 2014500019A5
- Authority
- JP
- Japan
- Prior art keywords
- acetylglucosaminyltransferase
- catalytic domain
- recombinant protein
- domain
- chrysosporium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000003197 catalytic effect Effects 0.000 claims 25
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims 21
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 21
- 241000123346 Chrysosporium Species 0.000 claims 14
- 239000002157 polynucleotide Substances 0.000 claims 12
- 102000040430 polynucleotide Human genes 0.000 claims 12
- 108091033319 polynucleotide Proteins 0.000 claims 12
- 108010070113 alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I Proteins 0.000 claims 10
- 108010073219 beta-1,3-galactosyl-0-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase Proteins 0.000 claims 10
- 230000002538 fungal effect Effects 0.000 claims 10
- 238000000034 method Methods 0.000 claims 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims 8
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 claims 7
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 claims 7
- 230000000694 effects Effects 0.000 claims 7
- 125000006850 spacer group Chemical group 0.000 claims 7
- 230000008685 targeting Effects 0.000 claims 7
- 241000223218 Fusarium Species 0.000 claims 6
- 101000829958 Homo sapiens N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase Proteins 0.000 claims 6
- 241001149964 Tolypocladium Species 0.000 claims 5
- 241001674013 Chrysosporium lucknowense Species 0.000 claims 4
- 241000226677 Myceliophthora Species 0.000 claims 4
- OVRNDRQMDRJTHS-RTRLPJTCSA-N N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-RTRLPJTCSA-N 0.000 claims 4
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 claims 4
- 241000228341 Talaromyces Species 0.000 claims 4
- 241000223259 Trichoderma Species 0.000 claims 4
- 230000001086 cytosolic effect Effects 0.000 claims 4
- 229950006780 n-acetylglucosamine Drugs 0.000 claims 4
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims 3
- 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 claims 3
- 101150039297 Alg3 gene Proteins 0.000 claims 3
- 241000228212 Aspergillus Species 0.000 claims 3
- 241000223651 Aureobasidium Species 0.000 claims 3
- 241001337994 Cryptococcus <scale insect> Species 0.000 claims 3
- 241001344133 Magnaporthe Species 0.000 claims 3
- 241000235395 Mucor Species 0.000 claims 3
- 241000223251 Myrothecium Species 0.000 claims 3
- 241000233892 Neocallimastix Species 0.000 claims 3
- 241000221960 Neurospora Species 0.000 claims 3
- 241000228143 Penicillium Species 0.000 claims 3
- 241000235379 Piromyces Species 0.000 claims 3
- 241000222480 Schizophyllum Species 0.000 claims 3
- 241001494489 Thielavia Species 0.000 claims 3
- 108020001778 catalytic domains Proteins 0.000 claims 3
- 102000037865 fusion proteins Human genes 0.000 claims 3
- 108020001507 fusion proteins Proteins 0.000 claims 3
- 150000004676 glycans Chemical class 0.000 claims 3
- 101150061302 och1 gene Proteins 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 102100021771 Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108010046068 N-Acetyllactosamine Synthase Proteins 0.000 claims 2
- 102000003838 Sialyltransferases Human genes 0.000 claims 2
- 108090000141 Sialyltransferases Proteins 0.000 claims 2
- 241001557886 Trichoderma sp. Species 0.000 claims 2
- 108010086873 UDP-N-acetylglucosamine transporter Proteins 0.000 claims 2
- 125000000539 amino acid group Chemical group 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 108010009689 mannosyl-oligosaccharide 1,2-alpha-mannosidase Proteins 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 101100184475 Candida albicans (strain SC5314 / ATCC MYA-2876) MNN24 gene Proteins 0.000 claims 1
- 101100130886 Candida albicans (strain SC5314 / ATCC MYA-2876) MNT1 gene Proteins 0.000 claims 1
- 108700023372 Glycosyltransferases Proteins 0.000 claims 1
- 102000051366 Glycosyltransferases Human genes 0.000 claims 1
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 claims 1
- 101001040734 Homo sapiens Golgi phosphoprotein 3 Proteins 0.000 claims 1
- 101001051674 Homo sapiens Meiosis-specific nuclear structural protein 1 Proteins 0.000 claims 1
- 241000223198 Humicola Species 0.000 claims 1
- 101100488151 Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) YEA4 gene Proteins 0.000 claims 1
- 101150093457 MNN2 gene Proteins 0.000 claims 1
- 101150075484 MNN9 gene Proteins 0.000 claims 1
- 102000001696 Mannosidases Human genes 0.000 claims 1
- 108010054377 Mannosidases Proteins 0.000 claims 1
- 108010087568 Mannosyltransferases Proteins 0.000 claims 1
- 102000006722 Mannosyltransferases Human genes 0.000 claims 1
- 102100024962 Meiosis-specific nuclear structural protein 1 Human genes 0.000 claims 1
- 102100023315 N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase Human genes 0.000 claims 1
- 108010056664 N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase Proteins 0.000 claims 1
- 101100454113 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) KRE2 gene Proteins 0.000 claims 1
- 101100181264 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) KTR6 gene Proteins 0.000 claims 1
- 101100457678 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) MNN10 gene Proteins 0.000 claims 1
- 101100184479 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) MNN4 gene Proteins 0.000 claims 1
- 101100406646 Schizosaccharomyces pombe (strain 972 / ATCC 24843) arg3 gene Proteins 0.000 claims 1
- 101150111043 VAN1 gene Proteins 0.000 claims 1
- 108010039255 alpha 1,6-mannosyltransferase Proteins 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 229910052705 radium Inorganic materials 0.000 claims 1
- HCWPIIXVSYCSAN-UHFFFAOYSA-N radium atom Chemical compound [Ra] HCWPIIXVSYCSAN-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41714410P | 2010-11-24 | 2010-11-24 | |
| US61/417,144 | 2010-11-24 | ||
| PCT/EP2011/070956 WO2012069593A2 (en) | 2010-11-24 | 2011-11-24 | Fusion enzymes |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014500019A JP2014500019A (ja) | 2014-01-09 |
| JP2014500019A5 true JP2014500019A5 (https=) | 2015-01-08 |
| JP6017439B2 JP6017439B2 (ja) | 2016-11-02 |
Family
ID=45093734
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013540368A Expired - Fee Related JP6017439B2 (ja) | 2010-11-24 | 2011-11-24 | Ｎ−アセチルグルコサミニルトランスフェラーゼ活性を有する融合酵素 |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US9399764B2 (https=) |
| EP (1) | EP2643456B1 (https=) |
| JP (1) | JP6017439B2 (https=) |
| KR (1) | KR20140018851A (https=) |
| CN (1) | CN103348003B (https=) |
| AU (1) | AU2011333685A1 (https=) |
| BR (1) | BR112013013045A2 (https=) |
| CA (1) | CA2819071A1 (https=) |
| DK (1) | DK2643456T3 (https=) |
| IL (1) | IL226481A0 (https=) |
| MX (1) | MX338560B (https=) |
| RU (1) | RU2013128619A (https=) |
| SG (1) | SG190321A1 (https=) |
| WO (1) | WO2012069593A2 (https=) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2643456B1 (en) | 2010-11-24 | 2016-05-11 | Novartis International Pharmaceutical Ltd. | Fusion enzymes having n-acetylglucosaminyltransferase activity |
| EP2852610B1 (en) | 2012-05-23 | 2018-07-11 | Glykos Finland Oy | Production of fucosylated glycoproteins |
| SG11201508347YA (en) * | 2013-03-14 | 2015-11-27 | Glycobia Inc | Oligosaccharide compositions, glycoproteins and methods to produce the same in prokaryotes |
| WO2015001049A1 (en) | 2013-07-04 | 2015-01-08 | Novartis Ag | O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof |
| SG11201600115SA (en) * | 2013-07-10 | 2016-02-26 | Novartis Ag | Multiple proteases deficient filamentous fungal cells and methods of use thereof |
| CA2916899C (en) | 2013-07-10 | 2020-03-10 | Novartis Ag | Production of glycoproteins having increased n-glycosylation site occupancy |
| EP3027741B1 (en) | 2013-07-29 | 2019-10-23 | Danisco US Inc. | Gh61 enzyme variants |
| SG11201700446XA (en) | 2014-07-21 | 2017-02-27 | Glykos Finland Oy | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
| CN105695439B (zh) * | 2014-11-27 | 2023-08-01 | 丰益(上海)生物技术研发中心有限公司 | 一种β-葡萄糖苷酶基因的重组表达方法 |
| JP2021534763A (ja) * | 2018-08-21 | 2021-12-16 | クララ フーズ カンパニー | 微生物におけるタンパク質のグリコシル化の修飾 |
| KR102202606B1 (ko) * | 2018-11-30 | 2021-01-15 | (주)바이오스플래시 | 바이오레티놀을 생산하는 미생물 및 이를 이용한 바이오레티놀의 생산방법 |
| CN111699252B (zh) * | 2019-01-16 | 2024-04-30 | 福尼亚生物处理股份有限公司 | 内切葡聚糖酶组合物和方法 |
| CN110448719B (zh) * | 2019-06-28 | 2020-05-22 | 浙江大学 | 一种促进凝血的丝素-多肽电纺膜及其制备方法 |
| CN116121094B (zh) * | 2023-03-03 | 2024-07-12 | 天津科技大学 | 一株高产神经酰胺的酿酒酵母工程菌株、构建方法及其应用 |
| CN119876300B (zh) * | 2025-03-28 | 2025-12-23 | 北京艾普希隆生物科技有限公司 | 一种制备唾液酸化普鲁兰多糖的方法 |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4500707A (en) | 1980-02-29 | 1985-02-19 | University Patents, Inc. | Nucleosides useful in the preparation of polynucleotides |
| US4475196A (en) | 1981-03-06 | 1984-10-02 | Zor Clair G | Instrument for locating faults in aircraft passenger reading light and attendant call control system |
| US4447233A (en) | 1981-04-10 | 1984-05-08 | Parker-Hannifin Corporation | Medication infusion pump |
| US4439196A (en) | 1982-03-18 | 1984-03-27 | Merck & Co., Inc. | Osmotic drug delivery system |
| US4447224A (en) | 1982-09-20 | 1984-05-08 | Infusaid Corporation | Variable flow implantable infusion apparatus |
| US4487603A (en) | 1982-11-26 | 1984-12-11 | Cordis Corporation | Implantable microinfusion pump system |
| US4486194A (en) | 1983-06-08 | 1984-12-04 | James Ferrara | Therapeutic device for administering medicaments through the skin |
| US4743546A (en) | 1985-02-13 | 1988-05-10 | Biotechnica International, Inc. | Controlled gene excision |
| US4596556A (en) | 1985-03-25 | 1986-06-24 | Bioject, Inc. | Hypodermic injection apparatus |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4790824A (en) | 1987-06-19 | 1988-12-13 | Bioject, Inc. | Non-invasive hypodermic injection device |
| US4941880A (en) | 1987-06-19 | 1990-07-17 | Bioject, Inc. | Pre-filled ampule and non-invasive hypodermic injection device assembly |
| US5700637A (en) | 1988-05-03 | 1997-12-23 | Isis Innovation Limited | Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays |
| GB8822228D0 (en) | 1988-09-21 | 1988-10-26 | Southern E M | Support-bound oligonucleotides |
| US5312335A (en) | 1989-11-09 | 1994-05-17 | Bioject Inc. | Needleless hypodermic injection device |
| US5064413A (en) | 1989-11-09 | 1991-11-12 | Bioject, Inc. | Needleless hypodermic injection device |
| US5383851A (en) | 1992-07-24 | 1995-01-24 | Bioject Inc. | Needleless hypodermic injection device |
| DE19754622A1 (de) | 1997-12-09 | 1999-06-10 | Antje Von Dr Schaewen | Pflanzliche GntI-Sequenzen und Verwendung derselben zur Gewinnung von Pflanzen mit verminderter oder fehlender N-Acetylglucosaminyltransferase I (GnTI)-Aktivität |
| US7244601B2 (en) * | 1997-12-15 | 2007-07-17 | National Research Council Of Canada | Fusion proteins for use in enzymatic synthesis of oligosaccharides |
| CN1197965C (zh) | 1998-10-26 | 2005-04-20 | 诺维信公司 | 在丝状真菌细胞内构建和筛选目的dna文库 |
| DE60032370T2 (de) * | 1999-08-19 | 2007-10-11 | Kirin Beer K.K. | Hefevarianten und verfahren zur herstellung von glykoprotein enthaltenden zuckerketten vom säugetiertyp |
| US7795002B2 (en) * | 2000-06-28 | 2010-09-14 | Glycofi, Inc. | Production of galactosylated glycoproteins in lower eukaryotes |
| US7598055B2 (en) * | 2000-06-28 | 2009-10-06 | Glycofi, Inc. | N-acetylglucosaminyltransferase III expression in lower eukaryotes |
| CA2471551C (en) * | 2001-12-27 | 2014-09-30 | Glycofi, Inc. | Methods to engineer mammalian-type carbohydrate structures |
| US7569376B2 (en) * | 2002-05-03 | 2009-08-04 | Neose Technologies, Inc. | Fucosyltransferase fusion protein |
| WO2006026992A1 (en) * | 2004-09-07 | 2006-03-16 | Novozymes A/S | Altered structure of n-glycans in a fungus |
| US7501275B2 (en) | 2004-10-27 | 2009-03-10 | The United States Of America As Represented By The Secretary Of Agriculture | Yeast transformation system |
| US20080026376A1 (en) | 2006-07-11 | 2008-01-31 | Huaming Wang | KEX2 cleavage regions of recombinant fusion proteins |
| MX2009003605A (es) | 2006-10-03 | 2009-04-22 | Metabolic Explorer Sa | Procedimiento para integracion cromosomica y reemplazo de secuencia de adn en clostridia. |
| TW201028431A (en) * | 2008-10-31 | 2010-08-01 | Lonza Ag | Novel tools for the production of glycosylated proteins in host cells |
| EP2643456B1 (en) | 2010-11-24 | 2016-05-11 | Novartis International Pharmaceutical Ltd. | Fusion enzymes having n-acetylglucosaminyltransferase activity |
-
2011
- 2011-11-24 EP EP11790938.2A patent/EP2643456B1/en not_active Not-in-force
- 2011-11-24 CA CA2819071A patent/CA2819071A1/en not_active Abandoned
- 2011-11-24 SG SG2013038088A patent/SG190321A1/en unknown
- 2011-11-24 MX MX2013005853A patent/MX338560B/es active IP Right Grant
- 2011-11-24 DK DK11790938.2T patent/DK2643456T3/en active
- 2011-11-24 KR KR1020137016445A patent/KR20140018851A/ko not_active Withdrawn
- 2011-11-24 AU AU2011333685A patent/AU2011333685A1/en not_active Abandoned
- 2011-11-24 WO PCT/EP2011/070956 patent/WO2012069593A2/en not_active Ceased
- 2011-11-24 BR BR112013013045-8A patent/BR112013013045A2/pt not_active IP Right Cessation
- 2011-11-24 RU RU2013128619/10A patent/RU2013128619A/ru not_active Application Discontinuation
- 2011-11-24 CN CN201180064714.3A patent/CN103348003B/zh not_active Expired - Fee Related
- 2011-11-24 JP JP2013540368A patent/JP6017439B2/ja not_active Expired - Fee Related
- 2011-11-24 US US13/989,084 patent/US9399764B2/en active Active
-
2013
- 2013-05-21 IL IL226481A patent/IL226481A0/en unknown
-
2016
- 2016-07-08 US US15/205,577 patent/US20170002337A1/en not_active Abandoned
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2014500019A5 (https=) | ||
| RU2013128619A (ru) | Слитные ферменты | |
| US10724063B2 (en) | Multiple proteases deficient filamentous fungal cells and methods of use thereof | |
| US10513724B2 (en) | Production of glycoproteins with mammalian-like N-glycans in filamentous fungi | |
| US7029872B2 (en) | Methods for producing modified glycoproteins | |
| US20220213451A1 (en) | O-Mannosyltransferase Deficient Filamentous Fungal Cells and Methods of Use Thereof | |
| JP2010519930A5 (https=) | ||
| US9695454B2 (en) | Production of fucosylated glycoproteins | |
| JP2012506710A5 (https=) | ||
| CN101679934A (zh) | 具有经修饰的岩藻糖基化的糖蛋白的生产 | |
| CN102725396A (zh) | 在缺乏二肽基氨肽酶活性的巴斯德毕赤酵母中生产治疗性蛋白的方法 | |
| CN104797703A (zh) | 产生显著均质的聚糖结构的巴斯德毕赤酵母菌株 | |
| JP2014532406A (ja) | 組換えタンパク質発現のための操作された下等真核宿主株 | |
| CN105492620A (zh) | 具有增加的n糖基化占位的糖蛋白的生产 | |
| JP5885191B2 (ja) | 糖鎖改変酵母及びそれを用いた糖タンパク質の製造方法 | |
| CN113549560B (zh) | 一种用于糖蛋白制备的工程化酵母构建方法及其菌株 | |
| US8003349B2 (en) | YLMPO1 gene derived from yarrowia lipolytica and a process for preparing a glycoprotein not being mannosylphosphorylated by using a mutated yarrowia lipolytica in which YLMPO1 gene is disrupted | |
| CN1918297B (zh) | 编码α1,6-甘露糖基转移酶的新的多形汉逊氏酵母基因和用同一基因有缺陷的多形汉逊氏酵母突变株生产重组糖蛋白的方法 | |
| US20250346853A1 (en) | Genetically-modified filamentous fungi for production of exogenous proteins having reduced or no n-linked glycosylation | |
| WO2007123801A1 (en) | Filamentous fungi having reduced udp-galactofuranose content |