JP2011520466A5 - - Google Patents
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- JP2011520466A5 JP2011520466A5 JP2011510512A JP2011510512A JP2011520466A5 JP 2011520466 A5 JP2011520466 A5 JP 2011520466A5 JP 2011510512 A JP2011510512 A JP 2011510512A JP 2011510512 A JP2011510512 A JP 2011510512A JP 2011520466 A5 JP2011520466 A5 JP 2011520466A5
- Authority
- JP
- Japan
- Prior art keywords
- seq
- residues
- luciferase
- amino acid
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108060001084 Luciferase Proteins 0.000 claims description 61
- 239000005089 Luciferase Substances 0.000 claims description 61
- 108091033319 polynucleotide Proteins 0.000 claims description 30
- 102000040430 polynucleotide Human genes 0.000 claims description 30
- 239000002157 polynucleotide Substances 0.000 claims description 30
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 27
- 229920001184 polypeptide Polymers 0.000 claims description 20
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 20
- 238000006467 substitution reaction Methods 0.000 claims description 17
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 16
- 238000004020 luminiscence type Methods 0.000 claims description 13
- 108090000331 Firefly luciferases Proteins 0.000 claims description 9
- 239000003153 chemical reaction reagent Substances 0.000 claims description 9
- 108020001507 fusion proteins Proteins 0.000 claims description 9
- 102000037865 fusion proteins Human genes 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 125000004122 cyclic group Chemical group 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 3
- 125000000539 amino acid group Chemical group 0.000 claims 2
- 238000012217 deletion Methods 0.000 claims 1
- 230000037430 deletion Effects 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 5
- 241000059559 Agriotes sordidus Species 0.000 description 4
- 241000963438 Gaussia <copepod> Species 0.000 description 4
- 108010052090 Renilla Luciferases Proteins 0.000 description 4
- 241000411414 Brachypterolus vestitus Species 0.000 description 3
- 102000003688 G-Protein-Coupled Receptors Human genes 0.000 description 3
- 108090000045 G-Protein-Coupled Receptors Proteins 0.000 description 3
- 241000270322 Lepidosauria Species 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 108700026244 Open Reading Frames Proteins 0.000 description 3
- 239000008186 active pharmaceutical agent Substances 0.000 description 3
- 150000007523 nucleic acids Chemical group 0.000 description 3
- 241000254158 Lampyridae Species 0.000 description 2
- 241001443978 Oplophorus Species 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US5437408P | 2008-05-19 | 2008-05-19 | |
| US61/054,374 | 2008-05-19 | ||
| US11998308P | 2008-12-04 | 2008-12-04 | |
| US61/119,983 | 2008-12-04 | ||
| PCT/US2009/003132 WO2009142735A2 (en) | 2008-05-19 | 2009-05-19 | LUCIFERASE BIOSENSORS FOR cAMP |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014266438A Division JP6275635B2 (ja) | 2008-05-19 | 2014-12-26 | ｃＡＭＰのためのルシフェラーゼバイオセンサー |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2011520466A JP2011520466A (ja) | 2011-07-21 |
| JP2011520466A5 true JP2011520466A5 (https=) | 2016-10-27 |
| JP6110591B2 JP6110591B2 (ja) | 2017-04-05 |
Family
ID=41202867
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011510512A Active JP6110591B2 (ja) | 2008-05-19 | 2009-05-19 | ｃＡＭＰのためのルシフェラーゼバイオセンサー |
| JP2014266438A Active JP6275635B2 (ja) | 2008-05-19 | 2014-12-26 | ｃＡＭＰのためのルシフェラーゼバイオセンサー |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014266438A Active JP6275635B2 (ja) | 2008-05-19 | 2014-12-26 | ｃＡＭＰのためのルシフェラーゼバイオセンサー |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US9045730B2 (https=) |
| EP (1) | EP2281046B1 (https=) |
| JP (2) | JP6110591B2 (https=) |
| AT (1) | ATE542903T1 (https=) |
| WO (1) | WO2009142735A2 (https=) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2541765A1 (en) | 2003-10-10 | 2004-10-01 | Promega Corporation | Luciferase biosensor |
| EP2327768B1 (en) | 2006-04-03 | 2015-09-09 | Promega Corporation | Permuted and nonpermuted luciferase biosensors |
| WO2009142735A2 (en) | 2008-05-19 | 2009-11-26 | Promega Corporation | LUCIFERASE BIOSENSORS FOR cAMP |
| LT3409764T (lt) * | 2009-05-01 | 2020-06-10 | Promega Corporation | Sintetinės oplophorus liuciferazės su sustiprinta šviesos išeiga |
| US8735559B2 (en) | 2010-05-11 | 2014-05-27 | Promega Corporation | Mutant protease biosensors with enhanced detection characteristics |
| US9290794B2 (en) | 2010-05-11 | 2016-03-22 | Promega Corporation | Mutant protease biosensors with enhanced detection characteristics |
| BR112013010487B1 (pt) | 2010-11-02 | 2021-02-02 | Promega Corporation | compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro |
| WO2012061477A1 (en) | 2010-11-02 | 2012-05-10 | Promega Corporation | Coelenterazine derivatives and methods of using same |
| WO2012071631A1 (en) | 2010-12-03 | 2012-06-07 | Gene Stream Pty Ltd | Improved light-emitting molecules |
| EP2825887A4 (en) | 2012-03-15 | 2015-08-19 | Montana Molecular Llc | GENETICALLY-CODED, FLUORESCENT SENSORS FOR THE DETECTION OF INTRA-CELLULAR SIGNALS BY DIACYLGLYCEROL SIGNAL PATHS |
| JP2015000021A (ja) * | 2013-06-14 | 2015-01-05 | 浜松ホトニクス株式会社 | 細胞内ｃＡＭＰ濃度を制御する方法 |
| JP6484987B2 (ja) | 2013-10-21 | 2019-03-20 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 |
| US11366114B2 (en) | 2013-11-04 | 2022-06-21 | Montana Molecular Llc | Genetically encoded fluorescent sensors for detecting ligand bias and intracellular signaling through cAMP pathways |
| US9927430B2 (en) | 2014-01-29 | 2018-03-27 | Promega Corporation | Pro-substrates for live cell applications |
| US9790537B2 (en) | 2014-01-29 | 2017-10-17 | Promega Corporation | Quinone-masked probes as labeling reagents for cell uptake measurements |
| JP6265020B2 (ja) | 2014-04-16 | 2018-01-24 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 |
| WO2018049241A1 (en) | 2016-09-09 | 2018-03-15 | Promega Corporation | Dual protected pro-coelenterazine substrates |
| WO2020050208A1 (ja) * | 2018-09-05 | 2020-03-12 | ヤマサ醤油株式会社 | Ｔｓｈ受容体に対する自己抗体活性の迅速測定方法及びキット |
| US20200292543A1 (en) * | 2019-03-12 | 2020-09-17 | Quidel Corporation | Compositions, kits, and methods for detecting autoantibodies |
| US20210318292A1 (en) * | 2020-04-13 | 2021-10-14 | Sensorium Bio, Inc. | System and Method to Detect Small Molecules |
| US20240060059A1 (en) | 2022-05-04 | 2024-02-22 | Promega Corporation | Circularly permuted dehalogenase variants |
| EP4519426A2 (en) | 2022-05-04 | 2025-03-12 | Promega Corporation | Split modified dehalogenase variants |
| WO2025100444A1 (ja) * | 2023-11-07 | 2025-05-15 | 株式会社コスミックコーポレーション | Ｔｓｈ活性測定用のレポーター細胞 |
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| NO864301L (no) | 1986-10-28 | 1988-04-29 | Tore Jahnsen | Dna-prober for human regulatorisk enhet av camp-avhengige proteinkinaser. |
| AU4809290A (en) | 1988-11-18 | 1990-06-12 | General Hospital Corporation, The | A camp-responsive transcriptional enhancer binding protein |
| US5683888A (en) | 1989-07-22 | 1997-11-04 | University Of Wales College Of Medicine | Modified bioluminescent proteins and their use |
| GB8916806D0 (en) | 1989-07-22 | 1989-09-06 | Univ Wales Medicine | Modified proteins |
| FR2682122B1 (fr) | 1991-10-03 | 1995-06-09 | Pasteur Institut | Nouveaux genes d'helicobacter pylori. leur utilisation pour la preparation de souches recombinantes de h. pylori. |
| EP1978091A1 (en) | 1994-01-03 | 2008-10-08 | Promega Corporation | Mutant luciferases |
| US6294330B1 (en) | 1997-01-31 | 2001-09-25 | Odyssey Pharmaceuticals Inc. | Protein fragment complementation assays for the detection of biological or drug interactions |
| CA2196496A1 (en) | 1997-01-31 | 1998-07-31 | Stephen William Watson Michnick | Protein fragment complementation assay for the detection of protein-protein interactions |
| US7312302B2 (en) | 1997-02-20 | 2007-12-25 | Oncolmmunin, Inc. | Compositions for the detection of enzyme activity in biological samples and methods of use thereof |
| US6037137A (en) | 1997-02-20 | 2000-03-14 | Oncoimmunin, Inc. | Fluorogenic peptides for the detection of protease activity |
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| CA2301828A1 (en) | 1997-09-19 | 1999-03-25 | Promega Corporation | Thermostable luciferases and methods of production |
| US6342611B1 (en) | 1997-10-10 | 2002-01-29 | Cytovia, Inc. | Fluorogenic or fluorescent reporter molecules and their applications for whole-cell fluorescence screening assays for capsases and other enzymes and the use thereof |
| US6884870B2 (en) | 1998-03-20 | 2005-04-26 | California Institute Of Technology | Fusion proteins for identifying proteases, protease target sites and regulators of protease activity in living cells |
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| AU1223200A (en) | 1998-10-23 | 2000-05-15 | Massachusetts Institute Of Technology | Genes integrating signal transduction pathways |
| GB9823468D0 (en) | 1998-10-28 | 1998-12-23 | Secr Defence | Novel enzyme |
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| JP3059435B1 (ja) | 1999-03-18 | 2000-07-04 | 扶桑薬品工業株式会社 | ｃＡＭＰおよびアデニル酸シクラ―ゼの酵素的蛍光定量アッセイ |
| US6573059B1 (en) | 1999-06-04 | 2003-06-03 | Rmf Dictagene S.A. | Use of the regulatory subunit of the camp dependent protein kinase (PKA) from dictyostelium for camp measurements |
| US7109315B2 (en) | 2000-03-15 | 2006-09-19 | Bruce J. Bryan | Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items |
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| KR20080050584A (ko) | 2005-08-19 | 2008-06-09 | 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 | 아라크노캄파 루시페라아제 |
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| WO2009140662A1 (en) | 2008-05-15 | 2009-11-19 | Illumina, Inc. | Engineered luciferases |
| WO2009142735A2 (en) | 2008-05-19 | 2009-11-26 | Promega Corporation | LUCIFERASE BIOSENSORS FOR cAMP |
| US8735559B2 (en) | 2010-05-11 | 2014-05-27 | Promega Corporation | Mutant protease biosensors with enhanced detection characteristics |
| JP5501862B2 (ja) | 2010-05-24 | 2014-05-28 | 株式会社サンユー | 金属被膜見本キット |
| BR112013010487B1 (pt) | 2010-11-02 | 2021-02-02 | Promega Corporation | compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro |
| EP3467119B1 (en) | 2011-11-11 | 2022-02-23 | Promega Corporation | Mutant protease biosensors with enhanced detection characteristics |
-
2009
- 2009-05-19 WO PCT/US2009/003132 patent/WO2009142735A2/en not_active Ceased
- 2009-05-19 JP JP2011510512A patent/JP6110591B2/ja active Active
- 2009-05-19 EP EP09750966A patent/EP2281046B1/en active Active
- 2009-05-19 AT AT09750966T patent/ATE542903T1/de active
- 2009-05-19 US US12/454,643 patent/US9045730B2/en active Active
-
2014
- 2014-12-26 JP JP2014266438A patent/JP6275635B2/ja active Active
-
2015
- 2015-04-24 US US14/695,944 patent/US9879306B2/en active Active
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