JP2011509073A5 - - Google Patents

Info

Publication number

JP2011509073A5

JP2011509073A5 JP2010538789A JP2010538789A JP2011509073A5 JP 2011509073 A5 JP2011509073 A5 JP 2011509073A5 JP 2010538789 A JP2010538789 A JP 2010538789A JP 2010538789 A JP2010538789 A JP 2010538789A JP 2011509073 A5 JP2011509073 A5 JP 2011509073A5

Authority

JP

Japan

Prior art keywords

polypeptide

population

amino acid

scaffold

affinity

Prior art date

2008-12-22

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
• Discuss

• 229920001184 polypeptide Polymers 0.000 claims 60
• 102000004196 processed proteins & peptides Human genes 0.000 claims 60
• 108090000765 processed proteins & peptides Proteins 0.000 claims 60
• 238000000034 method Methods 0.000 claims 16
• 102000040430 polynucleotide Human genes 0.000 claims 13
• 108091033319 polynucleotide Proteins 0.000 claims 13
• 239000002157 polynucleotide Substances 0.000 claims 13
• 125000003275 alpha amino acid group Chemical group 0.000 claims 9
• 125000000539 amino acid group Chemical group 0.000 claims 7
• 239000011324 bead Substances 0.000 claims 4
• 230000003993 interaction Effects 0.000 claims 4
• 238000004519 manufacturing process Methods 0.000 claims 4
• 150000001413 amino acids Chemical class 0.000 claims 3
• 239000003153 chemical reaction reagent Substances 0.000 claims 3
• 238000000926 separation method Methods 0.000 claims 3
• 230000008878 coupling Effects 0.000 claims 2
• 238000010168 coupling process Methods 0.000 claims 2
• 238000005859 coupling reaction Methods 0.000 claims 2
• NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 2
• 238000012514 protein characterization Methods 0.000 claims 2
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
• 102000004877 Insulin Human genes 0.000 claims 1
• 108090001061 Insulin Proteins 0.000 claims 1
• 108010006785 Taq Polymerase Proteins 0.000 claims 1
• 238000001514 detection method Methods 0.000 claims 1
• 229940039227 diagnostic agent Drugs 0.000 claims 1
• 239000000032 diagnostic agent Substances 0.000 claims 1
• 229940125396 insulin Drugs 0.000 claims 1
• 230000035772 mutation Effects 0.000 claims 1
• 108090000623 proteins and genes Proteins 0.000 claims 1
• 102220002405 rs121908660 Human genes 0.000 claims 1
• 238000012163 sequencing technique Methods 0.000 claims 1
• 230000002194 synthesizing effect Effects 0.000 claims 1
• 238000002560 therapeutic procedure Methods 0.000 claims 1